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| 1 | Expansion and differentiation of human hepatocyte-derived liver progenitor-like cells and their use for the study of hepatotropic pathogens显示文摘The study of pathophysiological mecha nisms in huma n liver disease has bee n constrained by the in ability to expa nd primary hepatocytes in vitro while maintaining proliferative capacity and metabolic function. We and others have previously shown that mouse mature hepatocytes can be con verted to liver progenitor-like cells in vitro with defined chemical factors. Here we describe a protocol achieving efficient conversion of human primary hepatocytes into liver progenitor-like cells (HepLPCs) through delivery of developmentally relevant cues, including NAD +-dependent deacetylase SIRT1 signaling. These HepLPCs could be expanded sign ifica ntly duri ng in vitro passage. The expa nded cells can readily be con verted back into metabolically functional hepatocytes in vitro and upon tran splantati on in vivo. Under three-dime nsional culture conditions, differentiated cells gen erated from HepLPCs regai ned the ability to support infect io n or reactivatio n of hepatitis B virus (HBV). Our work dem on strates the utility of the con versi on betwee n hepatocyte and liver proge nitor-like cells for studying HBV biology and an tiviral therapies. These fin dings will facilitate the study of liver diseases and regenerative medicine. | Gong-Bo Fu Wei-Jian Huang Min Zeng Xu Zhou Hong-Ping Wu Chang-Cheng Liu Han Wu Jun Weng Hong-Dan Zhang Yong-Chao Cai Charles Ashton Min Ding Dan Tang Bao-Hua Zhang Yi Gao Wei-Feng Yu Bo Zhai Zhi-Ying He Hong-Yang Wang He-Xin Yan | 2019 | Cell Research2019，29，1： | 18 |
| 2 | Effects of Shuanghuanglian oral liquids on patients with COVID-19:a randomized,open-label,parallel-controlled,multicenter clinical trial显示文摘We conducted a randomized,open-label,parallel-controlled,multicenter trial on the use of Shuanghuanglian(SHL),a traditional Chinese patent medicine,in treating cases of COVID-19.A total of 176 patients received SHL by three doses(56 in low dose,61 in middle dose,and 59 in high dose)in addition to standard care.The control group was composed of 59 patients who received standard therapy alone.Treatment with SHL was not associated with a difference from standard care in the time to disease recovery.Patients with 14-day SHL treatment had significantly higher rate in negative conversion of SARS-CoV-2 in nucleic acid swab tests than the patients from the control group(93.4%vs.73.9%,P=0.006).Analysis of chest computed tomography images showed that treatment with high-dose SHL significantly promoted absorption of inflammatory focus of pneumonia,which was evaluated by density reduction of inflammatory focus from baseline,at day 7(mean difference(95%CI),−46.39(−86.83 to−5.94)HU;P=0.025)and day 14(mean difference(95%CI),−74.21(−133.35 to−15.08)HU;P=0.014).No serious adverse events occurred in the SHL groups.This study illustrated that SHL in combination with standard care was safe and partially effective for the treatment of COVID-19. | Li Ni Zheng Wen Xiaowen Hu Wei Tang Haisheng Wang Ling Zhou Lujin Wu Hong Wang Chang Xu Xizhen Xu Zhichao Xiao Zongzhe Li Chene Li Yujian Liu Jialin Duan Chen Chen Dan Li Runhua Zhang Jinliang Li Yongxiang Yi Wei Huang Yanyan Chen Jianping Zhao Jianping Zuo Jianping Weng Hualiang Jiang Dao Wen Wang | 2021 | Frontiers of Medicine2021，15，5： | 7 |
| 3 | The first power generation test of hot dry rock resources exploration and production demonstration project in the Gonghe Basin,Qinghai Province,China显示文摘Hot dry rock(HDR)is a kind of clean energy with significant potential.Since the 1970s,the United States,Japan,France,Australia,and other countries have attempted to conduct several HDR development research projects to extract thermal energy by breaking through key technologies.However,up to now,the development of HDR is still in the research,development,and demonstration stage.An HDR exploration borehole(with 236℃ at a depth of 3705 m)was drilled into Triassic granite in the Gonghe Basin in northwest China in 2017.Subsequently,China Geological Survey(CGS)launched the HDR resources exploration and production demonstration project in 2019.After three years of efforts,a sequence of significant technological breakthroughs have been made,including the genetic model of deep heat sources,directional drilling and well completion in high-temperature hard rock,large-scale reservoir stimulation,reservoir characterization,and productivity evaluation,reservoir connectivity and flow circulation,efficient thermoelectric conversion,monitoring,and geological risk assessment,etc.Then the whole-process technological system for HDR exploration and production has been preliminarily established accordingly.The first power generation test was completed in November 2021.The results of this project will provide scientific support for HDR development and utilization in the future. | Er-yong Zhang Dong-guang Wen Gui-ling Wang Wei-de Yan Wen-shi Wang Cheng-ming Ye Xu-feng Li Huang Wang Xian-chun Tang Wei Weng Kuan Li Chong-yuan Zhang Ming-xing Liang Hong-bao Luo Han-yue Hu Wei Zhang Sen-qi Zhang Xian-peng Jin Hai-dong Wu Lin-you Zhang Qing-da Feng Jing-yu Xie Dan Wang Yun-chao He Yue-wei Wang Zu-bin Chen Zheng-pu Cheng Wei-feng Luo Yi Yang Hao Zhang En-lai Zha Yu-lie Gong Yu Zheng Chang-sheng Jiang Sheng-sheng Zhang Xue Niu Hui Zhang Li-sha Hu Gui-lin Zhu Wen-hao Xu Zhao-xuan Niu Li Yang | 2022 | China Geology2022，5，3： | 4 |
| 4 | Cis-2-dodecenoic Acid Mediates Its Synergistic Effect with Triazoles by Interfering with Efflux Pumps in Fluconazole-resistant Candida albicans显示文摘Objective To evaluate the synergy of the Burkholderia signaling molecule cis-2-dodecenoic acid(BDSF) and fluconazole(FLU) or itraconazole(ITRA) against two azole-resistant C. albicans clinical isolates in vitro and in vivo. Methods Minimum inhibitory concentrations(MICs) of antibiotics against two azole-resistant C. albicans were measured by the checkerboard technique, E-test, and time-kill assay. In vivo antifungal synergy testing was performed on mice. Analysis of the relative gene expression levels of the strains was conducted by quantitative reverse-transcription polymerase chain reaction(qR T-PCR). Results BDSF showed highly synergistic effects in combination with FLU or ITRA with a fractional inhibitory concentration index of ≤ 0.08. BDSF was not cytotoxic to normal human foreskin fibroblast cells at concentrations of up to 300 μg/mL. The qR T-PCR results showed that the combination of BDSF and FLU/ITRA significantly inhibits the expression of the efflux pump genes CDR1 and MDR1 via suppression of the transcription factors TAC1 and MRR1, respectively, when compared with FLU or ITRA alone. No dramatic difference in the mR NA expression levels of ERG1, ERG11, and UPC2 was found, which indicates that the drug combinations do not significantly interfere with UPC2-mediated ergosterol levels. In vivo experiments revealed that combination therapy can be an effective therapeutic approach to treat candidiasis. Conclusion The synergistic effects of BDSF and azoles may be useful as an alternative approach to control azole-resistant Candida infections. | YANG Dong Liang HU Yan Ling YIN Zi Xin ZENG Gui Sheng LI Dan ZHANG Yu Qian XU Zhen Hua GUAN Xiao Ming WENG Li Xing WANG Lian Hui | 2019 | Biomedical and Environmental Sciences2019，32，3： | 2 |
| 5 | B Cell-mediated Humoral Immunity in Chronic Hepatitis B Infection显示文摘B cell-mediated humoral immunity plays a vital role in viral infections,including chronic hepatitis B virus(HBV)infection,which remains a critical global public health issue.Despite hepatitis B surface antigen-specific antibodies are essential to eliminate viral infections,the reduced immune functional capacity of B cells was identified,which was also correlated with chronic hepatitis B(CHB)progression.In addition to B cells,T follicular helper(Tfh)cells,which assist B cells to produce antibodies,might also be involved in the process of anti-HBVspecific antibody production.Here,we provide a comprehensive review of the role of various subsets of B cells and Tfh cells during CHB progression and discuss current novel treatment strategies aimed at restoring humoral immunity.Understanding the mechanism of dysregulated B cells and Tfh cells will facilitate the ultimate functional cure of CHB patients. | Yang Li Shengxia Yin Rahma Issa Xin Tong Guiyang Wang Juan Xia Rui Huang Guangmei Chen Dan Weng Chen Chen Chao Wu Yuxin Chen | 2021 | Journal of Clinical and Translational Hepatology2021，9，4： | 2 |
| 6 | Calcipotriol abrogates cancer-associated fibroblast-derived IL-8-mediated oxaliplatin resistance in gastric cancer cells via blocking PI3K/Akt signaling显示文摘Activation of vitamin D receptor(VDR)in cancer-associated fibroblasts(CAFs)has been implicated in hesitating tumor progression and chemoresistance of several human malignancies.Yet,the role of VDR in CAF-induced chemotherapy resistance of gastric cancer(GC)cells remains elusive.In this study we first conducted immunohistochemistry analysis on tissue microarrays including 88 pairs of GC and normal mucosa samples,and provided clinical evidence that VDR was mainly expressed in gastric mucous cells but almost invisible in CAFs,and VDR expression was negatively correlated with malignant clinical phenotype and advanced stages,low VDR expression confers to poor overall survival rate of patients with GC.In a co-culture system of primary CAFs and cancer cells,we showed that treatment of HGC-27 and AGS GC cells with VDR ligand calcipotriol(Cal,500 nM)significantly inhibited CAF-induced oxaliplatin resistance.By using RNA-sequencing and Human Cytokine Antibody Array,we demonstrated that IL-8 secretion from CAFs induced oxaliplatin resistance via activating the PI3K/AKT pathway in GC,whereas Cal treatment greatly attenuated the tumor-supportive effect of CAF-derived IL-8 on GC cells.Taken together,this study verifies the specific localization of VDR in GC tissues and demonstrates that activation of VDR abrogates CAF-derived IL-8-mediated oxaliplatin resistance in GC via blocking PI3K/Akt signaling,suggesting vitamin D supplementation as a potential strategy of enhancing the anti-tumor effect of chemotherapy in GC. | Zhen-xiong Zhao Yan-qiu Zhang Hui Sun Zi-qi Chen Jin-jia Chang Xin Wang Xu Wang Cong Tan Shujuan Ni Wei-wei Weng Meng Zhang Lei Wang Dan Huang Yun Feng Wei-qi Shen Mi-die Xu | 2023 | Acta Pharmacologica Sinica2023，44，1： | 2 |
| 7 | Spectrum of toxic hepatitis following intentional paraquat ingestion: analysis of 187 cases显示文摘 | Chin‐Jung Yang Ja‐Liang Lin Dan‐Tzu Lin‐Tan Cheng‐Hao Weng Ching‐Wei Hsu Shen‐Yang Lee Shwu‐Hua Lee Chia‐Ming Chang Wey‐Ran Lin Tzung‐Hai Yen | 2012 | Liver Int2012，，9： | 1 |
| 8 | Re-detectable positive SARS-CoV-2 RNA tests in patients who recovered from COVID-19 with intestinal infection显示文摘Dear Editor,Severe acute respiratory syndrome coronavirus 2(SARSCoV-2),a novel coronavirus that causes Coronavirus Disease 2019(COVID-19)(Yang and Wang,2020),has spread to more than 200 countries and infected more than 9,000,000 people as of Jun 2020.Tens of thousands of patients with COVID-19 have recovered and been discharged from hospital.However,there are reports of recovered patients who subsequently tested positive for SARSCoV-2 after discharge(re-detectable positive,RP)(An et al.,2020;Lan et al.,2020),and this has led to increasing focus on the mechanism(s)underlying RP. | Wanyin Tao Xiaofang Wang Guorong Zhang Meng Guo Huan Ma Dan Zhao Yong Sun Jun He Lianxin Liu Kaiguang Zhang Yucai Wang Jianping Weng Xiaoling Ma Tengchuan Jin Shu Zhu | 2021 | Protein & Cell2021，12，3： | 1 |
| 9 | Reciprocal repression between TUSC 7 and mi R ‐23b in gastric cancer显示文摘 | Peng Qi Mi‐die Xu Xiao‐Han Shen Shu‐Juan Ni Dan Huang Cong Tan Wei‐Wei Weng Wei‐Qi Sheng Xiao‐Yan Zhou Xiang Du | 2015 | Cancer2015，，6： | 1 |
| 10 | TLSmell: Direct Identification on Malicious HTTPs Encryption Traffic withSimple Connection-Specific Indicators显示文摘Internet traffic encryption is a very common traffic protection method.Most internet traffic is protected by the encryption protocol called transport layersecurity (TLS). Although traffic encryption can ensure the security of communication, it also enables malware to hide its information and avoid being detected.At present, most of the malicious traffic detection methods are aimed at the unencrypted ones. There are some problems in the detection of encrypted traffic, suchas high false positive rate, difficulty in feature extraction, and insufficient practicability. The accuracy and effectiveness of existing methods need to be improved.In this paper, we present TLSmell, a framework that conducts maliciousencrypted HTTPs traffic detection with simple connection-specific indicators byusing different classifiers based online training. We perform deep packet analysisof encrypted traffic through data pre-processing to extract effective features, andthen the online training algorithm is used for training and prediction. Withoutdecrypting the original traffic, high-precision malicious traffic detection and analysis are realized, which can guarantee user privacy and communication security.At the same time, since there is no need to decrypt the traffic in advance, the effi-ciency of detecting malicious HTTPs traffic will be greatly improved. Combinedwith the traditional detection and analysis methods, malicious HTTPs traffic isscreened, and suspicious traffic is further analyzed by the expert through the context of suspicious behaviors, thereby improving the overall performance of malicious encrypted traffic detection. | Zhengqiu Weng Timing Chen Tiantian Zhu Hang Dong Dan Zhou Osama Alfarraj | 2021 | Computer Systems Science & Engineering2021，37，4： | 1 |
| 11 | Novel Photo-polymerizations Initiated by Alkyl Radicals Generated fromPhotocataylzed Decarboxylation of Carboxylic Acids overOxide Semiconductor Nanoparticles: Extended Photo-KolbeReactios 显示文摘 | Weng Zhen Ni Xiuyuan Yang Dan | 2009 | J Photochem Photobiol A2009，201，23： | 1 |
| 12 | Long Non-Coding RNA LSINCT5 Predicts Negative Prognosis and Exhibits Oncogenic Activity in Gastric Cancer显示文摘 | Mi-Die Xu Peng Qi Wei-Wei Weng Xiao-Han Shen Shu-Juan Ni Lei Dong Dan Huang Cong Tan Wei-Qi Sheng Xiao-Yan Zhou Xiang Du | 2014 | Medicine2014，，： | 1 |
| 13 | Specific cleavage of Mcl-1 by caspase-3 in tumor necrosis factor-related apoptosisinducing ligand (TRAIL)-induced apoptosis in Jurkat leukemia T cells显示文摘 | Weng Chang-jiang Li Yuan Xu Dan | 2005 | J Biol Chem2005，280，10： | 1 |
| 14 | Integrated scanning spectrometer with a tunable micro-ring resonator and an arrayed waveguide grating显示文摘Integrated spectrometers with both wide optical bandwidths and high spectral resolutions are required in applications such as spectral domain optical coherence tomography(SD-OCT).Here we propose a compact integrated scanning spectrometer by using a tunable micro-ring resonator(MRR)integrated with a single arrayed waveguide grating for operation in the 1265-1335-nm range.The spectral resolution of the spectrometer is determined by the quality factor of the MRR,and the optical bandwidth is defined by the free spectral range of the arrayed waveguide grating.The spectrometer is integrated with on-chip germanium photodetectors,which enable direct electrical readout.A 70-nm optical bandwidth and a 0.2-nm channel spacing enabled by scanning the MRR across one free spectral range are demonstrated,which offer a total of350 wavelength channels with 31-kHz wavelength scanning speed.The integrated spectrometer is applied to measure different spectra and the interference signals from an SD-OCT system,which shows its great potential for future applications in sensing and imaging systems. | ZUNYUE ZHANG YI WANG JIARUI WANG DAN YI DAVID WENG U CHAN WU YUAN HON KI TSANG | 2022 | Photonics Research2022，10，5： | 1 |
| 15 | The NLRP12 Inflammasome Recognizes Yersinia pestis显示文摘 | Gregory I. Vladimer Dan Weng Sara W. Montminy Paquette Sivapriya Kailasan Vanaja Vijay A.K. Rathinam Marie Hjelmseth Aune Joseph E. Conlon Joseph J. Burbage Megan K. Proulx Qin Liu George Reed Joan C. Mecsas Yoichiro Iwakura John Bertin Jon D. Goguen Kath | 2012 | Immunity2012，，： | 1 |
| 16 | Specific cleavage of Mcl-1 by caspase-3 in tumor necrosis factor-related apoptosis in- ducing ligand (TRAIL)-induced apoptosis in Jurkat leukemia T cells 显示文摘 | Weng Chang-jiang Yuan Li Xu Dan | 2005 | J Biol Chem2005，280，10： | 1 |
| 17 | Reciprocal repression between TUSC 7 and mi R ‐23b in gastric cancer显示文摘 | Peng Qi Mi‐die Xu Xiao‐Han Shen Shu‐Juan Ni Dan Huang Cong Tan Wei‐Wei Weng Wei‐Qi Sheng Xiao‐Yan Zhou Xiang Du | 2015 | Int. J. Cancer2015，，6： | 1 |
| 18 | Hydrothermal synthesis of copper complexes of 4′-pyridyl terpyridine: From discrete monomer to zigzag chain polymer显示文摘 | Hua Feng Xiao-Ping Zhou Tao Wu Dan Li Ye-Gao Yin Seik Weng Ng | 2006 | Inorganica Chimica Acta2006，，12： | 1 |
| 19 | TiOz nanorod films grown on Si wafers by a nanodot-assisted hy- drothermalgrowth 显示文摘 | TANG Dan CHENG Kui WENG Wenjian | 2011 | Thin Solid Films2011，519，： | 1 |
| 20 | Epidemiology of pancreatic cancer:New version,new vision显示文摘Pancreatic cancer(PC)is a devastating malignancy with an extremely high mortality rate and poses significant challenges to healthcare systems worldwide.The prevalence of PC risk factors spiked over the years,leading to a global increase in PC incidence rates.The contribution of different risk factors,however,varied from region to region due to genetic predisposition,environmental,social,and political factors underlying disease prevalence in addition to public health strategies.This comprehensive review aims to provide a thorough analysis of the epidemiology of PC,discussing its incidence,risk factors,screening strategies and socioeconomic burden.We compiled a wide range of seminal studies as well as epidemiological investigations to serve this review as a comprehensive guide for researchers,healthcare professionals,and policymakers keen for a more profound understanding of PC epidemiology.This review highlights the essentiality of persistent research efforts,interdisciplinary collaboration,and public health initiatives to address the expanding burden of this malignancy. | Wenhao Luo Jun Wang Hao Chen Liyuan Ye Jiangdong Qiu Yueze Liu Ruobing Wang Guihu Weng Tao Liu Dan Su Jinxin Tao Chen Ding Lei You Taiping Zhang | 2023 | Chinese Journal of Cancer Research2023，35，5： | 0 |
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