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| 1 | Luciferase reporter phage phAE85 for rapid detection of rifampicin resistance in clinical isolates of Mycobacterium tuberculosis显示文摘Objective:To evaluate luciferase reporter phage(LRP)phAE85 in rapid detection of rifampicin resistance in a region where TB is endemic.Methods:One hundred and ninety primary isolates on Lowenstein-Jensen medium were tested.Middlebrook 7H9 complete medium with and without rifampicin at 2μg/mL was inoculated with standard inoculum from suspensions of the clinical isolate.After incubation for 72 h,LRP was added.Following 4 h of further incubation,light output from both control and test was measured as relative light units.Strains exhibiting a reduction of less than 50%relative light units in the drug containing vial compared to control were classified as resistant.Results were compared with the conventional minimum inhibitory concentration method(MIC)of drug susceptibility testing.Results:The two methods showed high level of agreement of 97%(CI 0.94,0.99)and P value was 0.000 1.The sensitivity and specificity of LRP assay for detection of rifampicin resistance were 91%h(CI 0.75,0.98)and 99ct(CI0.95,1.00)respectively.Time to detection of resistance by LRP assay was 3 d in comparison with 28 d by the minimum inhibitory concentration method.Conclusions:LRP assay with phAE85 is 99%specific,91%sensitive and is highly reproducible.Thus the assay offers a simple procedure for drug sensitivity testing,within die scope of semi-automation. | Gomathi Sivaramakrishnan Balaji Subramanyam Ponnuraja C Vanaja Kumar | 2013 | Asian Pacific Journal of Tropical Medicine2013，6，9： | 4 |
| 2 | Potential applications of lactic acid bacteria and bacteriocins in antimycobacterial therapy显示文摘Tuberculosis(TB) is a communicable disease caused by Mycobacterium tuberculosis(M. tuberculosis). WHO estimated that 10.4 million new(incident) TB cases worldwide in year 2016. The increased prevalence of drug resistant strains and side effects associated with the current anti-tubercular drugs make the treatment options more complicated. Hence, there are necessities to identify new drug candidates to fight against various sub-populations of M. tuberculosis with less or no toxicity/side effects and shorter treatment duration. Bacteriocins produced by lactic acid bacteria(LAB) attract attention of researchers because of its 'Generally recognized as safe' status. LAB and its bacteriocins possess an effective antimicrobial activity against various bacteria and fungi. Interestingly bacteriocins such as nisin and lacticin 3147 have shown antimycobacterial activity in vitro. As probiotics, LAB plays a vital role in promoting various health benefits including ability to modulate immune response against various infectious diseases. LAB and its metabolic products activate immune system and thereby limiting the M. tuberculosis pathogenesis. The protein and peptide engineering techniques paved the ways to obtain hybrid bacteriocin derivatives from the known peptide sequence of existing bacteriocin. In this review, we focus on the antimycobacterial property and immunomodulatory role of LAB and its metabolic products. Techniques for large scale synthesis of potential bacteriocin with multifunctional activity and enhanced stability are also discussed. | Anbarasu Sivaraj Revathy Sundar Radhakrishnan Manikkam Krupakar Parthasarathy Uma Rani Vanaja Kumar | 2018 | Asian Pacific Journal of Tropical Medicine2018，11，8： | 2 |
| 3 | A brief review on experimental fluorosis显示文摘 | Ekambaram Perumal Vanaja Paul Vimal Govindarajan Lakshmikanthan Panneerselvam | 2013 | Toxicology Letters2013，，： | 2 |
| 4 | Synthesis and characterization of epoxy-novolac/bismaleimide networks显示文摘 | Vanaja A Rao R M V G K | 2002 | European Polymer Journal2002，38，1： | 1 |
| 5 | Bacterial Outer Membrane Vesicles Mediate Cytosolic Localization of LPS and Caspase-11 Activation 显示文摘 | Vanaja SK Russo AJ Behl B | 2016 | Ce112016，165，5： | 1 |
| 6 | Synthesis,antimyeobaeterial activity evaluation, and QSAR studies of chalcone derivatives 显示文摘 | P M Sivakumar S Prabu Seenivasan Vanaja Kumar Mukesh Doble | 2007 | Bioorganic & Medicinal Chemistry Letters2007，17，6： | 1 |
| 7 | Phenethyl Isothiocyanate Induces Cell Cycle Arrest and Reduction of Alpha-and Beta-Tubnlin Isotypes in Human Prostate Cancer Cells显示文摘 | Yin P Kawamura T He M Vanaja D K Young C Y | 2009 | Cell Biol Int2009，33，： | 1 |
| 8 | Mechanisms of inflammasome activation: recent advances and novel insights 显示文摘 | Vanaja SK Rathinam VA Fitzgerald KA | 2015 | Trends Cell Biol2015，25，5： | 1 |
| 9 | Synthesis and characterisation of epoxy-novolac/bismaleimide networks显示文摘 | Vanaja A Rao R M V | 2002 | European polymer journal2002，38，1： | 1 |
| 10 | Development in therapeutic importance of most ught marine algal Polysaccharide Fucoidans 显示文摘 | Rajeshkumar S Vanaja M Malarkodi C | 2013 | International Journal of Research in Biomedicine and Biotechnology2013，3，2： | 1 |
| 11 | Tanscriptional silencing of zinc finger protein 185 identified by expression profiling is associated with prostate cancer progression显示文摘 | Vanaja DK Cheville JC Iturria SJ | 2003 | Cancer Res2003，63，： | 1 |
| 12 | Synthesis,antimycobacterial activity evaluation,and QSAR studies of chalcone derivatives显示文摘 | SIVAKUMAR P M PRABU SEENIVASAN S VANAJA KUMAR | 2007 | Bioorganic & Medicinal Chemistry Letters2007，17，： | 1 |
| 13 | Mechanisms of inflammasome activation: recent advances and novel insights显示文摘 | Vanaja SK Rathinam VAK Fitzgerald KA | 2015 | Trends in Cell Biology2015，25，5： | 1 |
| 14 | Effect of geldanamycin on androgen receptor function and stability显示文摘 | Vanaja DK Mitchell SH Toft DO | 2002 | Cell Stress Chaperones2002，7，1： | 1 |
| 15 | Optimization of protein precipitation based upon effectiveness of protein removal and ionization effect in liquid chromatography–tandem mass spectrometry显示文摘 | Cara Polson Pratibha Sarkar Bev Incledon Vanaja Raguvaran Russell Grant | 2002 | Journal of Chromatography B2002，，2： | 1 |
| 16 | Tumor prevention and antitumor immunity with heat shock protein 70 induced by 15-deoxy-delta 12,14-prostaglandin j2 in transgenic adenomcarcinoma of mouse prostate cells显示文摘 | Vanaja KD Grossmann ME Celis E | | 0，，： | 1 |
| 17 | Regulation of inilammasomesignaling显示文摘 | Rathinam VA Vanaja SK Fitzgerald KA | 2012 | Nat Immunol2012，13，4： | 1 |
| 18 | Effect of oxygen intercalation on properties of sputtered CuYO2 for potential use as p-type transparent conducting films显示文摘 | MANOJ R NISHA M VANAJA K A | 2008 | Bulletin of Materials Science2008，31，1： | 1 |
| 19 | Molecular cloning and charac- terization of a novel transglutaminase cDNA from a hu- man prostate cDNA library显示文摘 | Grant F J Taylor D A Sheppard P O Mathewes S L Lint W Vanaja E | 1994 | Biochem Biophys Res Commun1994，203，： | 1 |
| 20 | Effect of geldanamycin on androgen receptor function and stability显示文摘 | Vanaja DK Mitchell SH Toft DO | 2002 | Cell Stress Chaperones2002，7，1： | 1 |
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