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| 1 | 厚朴及其成分的生物活性和毒性(英文)显示文摘中草药已在中国药典中使用了数千年。木兰属的厚朴,主要用于治疗焦虑、哮喘、抑郁症、胃肠道疾病、头痛。其树皮提取物是目前市售的食品补充剂和化妆品的主要成分。厚朴及其主要成分具有多种药理活性,具有抗氧化、抗炎、抗生素和抗痉挛作用。然而,相关机制尚未明确,临床试验也鲜有报道。体外和体内毒性研究已经发现了一些有趣的特征。本文旨在总结关于厚朴的成分、利用、药理学和安全性的文献。 | Melanie POIVRE Pierre DUEZ | 2017 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2017，18，3： | 10 |
| 2 | Chemical constituents, cytotoxic, antifungal and antimicrobial properties of Centaurea diluta Ait. subsp. algeriensis(Coss. & Dur.) Maire显示文摘Objective:To investigate the chemical composition of a moderately polar extract(CHC1_3 soluble part of the MeOH-H_2O extract) obtained from the aerial parts(leaves and flowers) of Centaurea diluta Ait.subsp.algeriensis(Coss.& Dur.) Maire,a species endemic to Algeria and Morocco on which no reports are available to date.To evaluate in vitro the cytotoxic,antifungal and antimicrobial activities of this extract and the cytotoxic and antimicrobial activities of its isolated secondary metabolites.Methods:The cytotoxic effects of the extract were investigated on 3 human cancer cell lines i.e.the A549 non-small-cell lung carcinoma(NSCLC),the MCF7 breast adenocarcinoma and the U373 glioblastoma using a MTT colorimetric assay.Biological data allowed to guide the fractionation of the extract by separation and purification on silica gel 60(CC and TLC).The isolated compounds which were characterized by spectral analysis,mainly HR-ESIMS,HR-EIMS,UV and NMR experiments(~1H,^(13)C,COSY,ROESY,HSQC and HMBC) and comparison of their spectroscopic data with those reported in the literature,were evaluated for cytotoxic activities on six cancer cell lines(A549,MCF7,U373,Hs683 human glioma,PC3 human prostate and B16-F10 murine melanoma).The direct and indirect antibacterial and antifungal activities were determined using microdilution methods for the raw extract and TLC-bioautography and microdilution methods against standard and clinical strains for the isolated compounds.Results:The raw extract reduced cell viability with IC_(50)s of 27,25 and 21 μg/mL on A549,MCF7 and U373,respectively.Five secondary metabolites:two phenolic compounds(vanillin 1,paridol 3),a lignan[(-)-arctigenin 2]and two flavonoid aglycones(eupatilin 4 and jaceosidin 5),were then isolated from this extract.Moderate cytotoxic effects were observed for(-)-arctigenin 2(IC_(50)s:28 and 33μM on Hs683 and B16-F10,respectively),eupatilin 4(IC_(50)s:33 and 47 μM on B16-F10 and PC3,respectively) and jaceosidin 5(IC_(50)s:32 and 40 μM on PC3 and B16-F10,respectively).Conclusions:All the isolated compounds were described for the first time from this species.Although inactive against 7 tested microorganisms(fungi,bacteria and yeast,human or plant pathogens),the raw extract was able to potentiate the effect of beta-lactam antibiotics on methicillin-resistant Staphylococcus aureus(MRSA),reducing the minimal inhibitory concentrations(MICs) by a factor of 2-32-fold.No synergy was found between the extract and streptomycin.From the five isolated compounds only jaseosidin 5 showed a moderate antimicrobial activity. | Hanene Zater Joelle Huet Veronique Fontaine Samir Benayache Caroline Stevigny Pierre Duez Fadila Benayache | 2016 | Asian Pacific Journal of Tropical Medicine2016，9，6： | 2 |
| 3 | Investigation on the Mode of Action of the Traditional Chinese Medical Prescription-Yiqihuoxue Formula, an Effective Extravasation Treatment for Cerebral Vascular Microemboli in ApoE-/- mice显示文摘Objective: The objective of this study was to investigate the mechanisms underlying anti-embolism and extravasational effects of traditional Chinese medical prescription YiqiHuoxue(YQHX) formula in ApoE-/-mice with cerebral vascular microemboli. Materials and Methods: An ApoE-/-mice model with microemboli was developed by infusing fluorescently labeled heterologous fibrin-rich microparticles into the internal carotid artery of ApoE -/-gene knockout male mice through the common carotid artery. Before microemboli injection, the animals were randomly divided into four groups of 10 animals, treated daily for 6 weeks by intragastric administration: The ApoE-/-control group(physiological saline, 0.2 mL/10 g/d), YQHX group(0.2 ml/10 g/d), clopidogrel group(3 mg/kg/d), and atorvastatin group(3 mg/kg/d);a further group was constituted of normal male C57 BL/6 J mice(with the same genetic background as ApoE-/-mice;normal control group;no treatment;microemboli injection). The mice in each microemboli group were divided into three subgroups, the 2-h, 24-h, and 72-h subgroups, corresponding to the time after microemboli injection. Two hours(or 24 h or 72 h) after microemboli injection, the changes in aortic intima and brain tissue were analyzed by histopathology, the amounts of fluorescent emboli being measured by fluorescence microscopy image analysis. Comparison points included the microemboli induced loss of aorta functions and pathological changes, atherosclerotic plaque, brain ultrastructure and functions, and embolus extravasation. Results: Loss of aorta functions and adverse pathological changes, atherosclerotic plaque, serious damage in brain ultrastructure and functions, and reduced thrombus elimination were obviously serious in microemboli injected ApoE-/-mice. These symptoms were significantly relieved by the YQHX pretreatment:(i) the ratio of thrombus accumulation was increased with a significant decrease in thrombus extravasation in ApoE-/-mice, while YQHX induced an increased thrombus extravasation;(ii) the degree of aortic intimal thickening and brain tissue structural disorders were significantly increased in ApoE-/-mice, but overtly inhibited in the YQHX group;(iii) YQHX restored cell viability and homeostasis in the brain;(iv) YQHX regulated the expression of pro-and anti-inflammatory cytokines in the aorta;and(v) YQHX reduced cortical nerve nuclei pyknosis, edema, liquefaction, and necrosis induced by brain hypoxia, especially in the 24 h and 72 h groups. Conclusions: These findings indicate that the protective effects of YQHX on the brain against microemboli-induced injury may be attributed to the activation of extravasation mechanisms, which are involved in the cerebrovascular injury pathway and constitutively important in the progression of ischemic stroke. | Chao Jiang Ting Wang Zhong-Ju Xu Xu Chao Pierre Duez | 2020 | World Journal of Traditional Chinese Medicine2020，6，1： | 2 |
| 4 | Fibrates suppress bile acid synthesis via peroxisome proliferator -activated receptor -alpha -mediated downregulation of cholesterol 7alpha - hydroxylase and sterol 27 - hydroxylase expression显示文摘 | Post SM Duez H Gervois PP | 2001 | Arterioscler Thromb Vasc Biol2001，21，11： | 1 |
| 5 | Statin induction of liver fatty acid-binding protein(L-FABP)gene expression is peroxisome proliferator-activated receptor-alpha-dependent显示文摘 | Landrier JF Thomas C Grober J Duez H Percevault F Souidi M | 2004 | J Biol Chem2004，279，45： | 1 |
| 6 | Peroxisome proliferator activated receptors and atherogenesis:regulators of gene expression in vascular cells显示文摘 | Marx N Duez H Fruchart JC | 2004 | Circ Res2004，94，9： | 1 |
| 7 | Effects of adding fenofibrate(2 00mg/d) in patient with combined hyperlipidemia and metabolic syndrome显示文摘 | Vega GL Duez H Blanquart C | 2003 | Am J Cardiol2003，91，： | 1 |
| 8 | Bile acid-activated nuclear receptor FXR suppresses apolipoprotein A-I transcrip- tion via a negative FXR response element 显示文摘 | Claudel T Sturm E Duez H | 2002 | J Clin Invest2002，109，： | 1 |
| 9 | Making a splash with water repellency显示文摘 | Duez C Ybert C Clanet C | 2007 | Nat Phys2007，，3： | 1 |
| 10 | Rev-erb alpha gives a time cue to metabolism 显示文摘 | Duez H Staels B | 2008 | FEBSLetters2008，582，1： | 1 |
| 11 | Bile acid-activated nuclear receptor FXR suppresses apolipoprotein AI transcription via a negative FXR response element显示文摘 | Claudel T Sturm E Duez H Torra IP Sirvent A Kosykh V | | 0，，： | 1 |
| 12 | Silent and non-silent pauses in three speech styles 显示文摘 | Duez D | 1982 | Language and Speech1982，25，1： | 1 |
| 13 | Regulation of human apoA-I by gemfibrozil and fenofibrate through selective peroxisome proliferator-activated receptor alpha modulation显示文摘 | Duez H Lefebvre B Poulain P | | 0，，03： | 1 |
| 14 | Silent and non-silent pauses in three speech styles 显示文摘 | Duez D | 1982 | Language and Speech1982，25，： | 1 |
| 15 | Peroxisome proliferator-activated receptors and atherogenesis: regulators of gene expression in vascular cells显示文摘 | Marx N Duez H Fruchart JC | 2004 | Circ Res2004，94，9： | 1 |
| 16 | Preoperative Radiotherapy as Adjuvant Treatment in Rectal Cancer: Final Results of a Randomized Study of the European Organization for Research and Treatment of Cancer (EORTC)显示文摘 | ANDRé GéRARD MARC BUYSE BERNARD NORDLINGER JEAN LOYGUE FRAN?OISE PèNE PETER KEMPF JEAN-FRAN?OIS BOSSET MARC GIGNOUX JEAN-PIERRE ARNAUD CLAUDE DESAIVE NICOLE DUEZ | 1988 | Annals of Surgery1988，，5： | 1 |
| 17 | Statistics of the comet assay: a key to discriminate between genotoxic effects 显示文摘 | Duez P Dehon G Kumps A | 2003 | Mutagenesis2003，18，： | 1 |
| 18 | Fibrates suppress bile acid synthesis via peroxisome proliferator-activated receptor-α-mediated downregulation of cholesterol 7α-hydroxylase and sterol 27-hydroxylase expression显示文摘 | Post SM Duez H Gervois PP | 2001 | Arterioscler Thromb Vasc Biol2001，21，11： | 1 |
| 19 | Cyclostationarity of acoustic emissions(AE) for monitoring bearing defects显示文摘 | KILUNDU B CHIEMENTIN X DUEZ J | 2011 | Mechanical Systems and Signal Processing2011，25，6： | 1 |
| 20 | One new LEN enzyme and two new OKP enzymes in Klebsiella pneumoniae clinical isolates and proposed nomenclature for chromosomal β-lactamases of this species 显示文摘 | Siebor E Pechinot A Duez JM etal | 2005 | Antimicrob Agents Chemother2005，49，7： | 1 |
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