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| 1 | 厚朴及其成分的生物活性和毒性(英文)显示文摘中草药已在中国药典中使用了数千年。木兰属的厚朴,主要用于治疗焦虑、哮喘、抑郁症、胃肠道疾病、头痛。其树皮提取物是目前市售的食品补充剂和化妆品的主要成分。厚朴及其主要成分具有多种药理活性,具有抗氧化、抗炎、抗生素和抗痉挛作用。然而,相关机制尚未明确,临床试验也鲜有报道。体外和体内毒性研究已经发现了一些有趣的特征。本文旨在总结关于厚朴的成分、利用、药理学和安全性的文献。 | Melanie POIVRE Pierre DUEZ | 2017 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2017，18，3： | 10 |
| 2 | Chemical constituents, cytotoxic, antifungal and antimicrobial properties of Centaurea diluta Ait. subsp. algeriensis(Coss. & Dur.) Maire显示文摘Objective:To investigate the chemical composition of a moderately polar extract(CHC1_3 soluble part of the MeOH-H_2O extract) obtained from the aerial parts(leaves and flowers) of Centaurea diluta Ait.subsp.algeriensis(Coss.& Dur.) Maire,a species endemic to Algeria and Morocco on which no reports are available to date.To evaluate in vitro the cytotoxic,antifungal and antimicrobial activities of this extract and the cytotoxic and antimicrobial activities of its isolated secondary metabolites.Methods:The cytotoxic effects of the extract were investigated on 3 human cancer cell lines i.e.the A549 non-small-cell lung carcinoma(NSCLC),the MCF7 breast adenocarcinoma and the U373 glioblastoma using a MTT colorimetric assay.Biological data allowed to guide the fractionation of the extract by separation and purification on silica gel 60(CC and TLC).The isolated compounds which were characterized by spectral analysis,mainly HR-ESIMS,HR-EIMS,UV and NMR experiments(~1H,^(13)C,COSY,ROESY,HSQC and HMBC) and comparison of their spectroscopic data with those reported in the literature,were evaluated for cytotoxic activities on six cancer cell lines(A549,MCF7,U373,Hs683 human glioma,PC3 human prostate and B16-F10 murine melanoma).The direct and indirect antibacterial and antifungal activities were determined using microdilution methods for the raw extract and TLC-bioautography and microdilution methods against standard and clinical strains for the isolated compounds.Results:The raw extract reduced cell viability with IC_(50)s of 27,25 and 21 μg/mL on A549,MCF7 and U373,respectively.Five secondary metabolites:two phenolic compounds(vanillin 1,paridol 3),a lignan[(-)-arctigenin 2]and two flavonoid aglycones(eupatilin 4 and jaceosidin 5),were then isolated from this extract.Moderate cytotoxic effects were observed for(-)-arctigenin 2(IC_(50)s:28 and 33μM on Hs683 and B16-F10,respectively),eupatilin 4(IC_(50)s:33 and 47 μM on B16-F10 and PC3,respectively) and jaceosidin 5(IC_(50)s:32 and 40 μM on PC3 and B16-F10,respectively).Conclusions:All the isolated compounds were described for the first time from this species.Although inactive against 7 tested microorganisms(fungi,bacteria and yeast,human or plant pathogens),the raw extract was able to potentiate the effect of beta-lactam antibiotics on methicillin-resistant Staphylococcus aureus(MRSA),reducing the minimal inhibitory concentrations(MICs) by a factor of 2-32-fold.No synergy was found between the extract and streptomycin.From the five isolated compounds only jaseosidin 5 showed a moderate antimicrobial activity. | Hanene Zater Joelle Huet Veronique Fontaine Samir Benayache Caroline Stevigny Pierre Duez Fadila Benayache | 2016 | Asian Pacific Journal of Tropical Medicine2016，9，6： | 2 |
| 3 | Investigation on the Mode of Action of the Traditional Chinese Medical Prescription-Yiqihuoxue Formula, an Effective Extravasation Treatment for Cerebral Vascular Microemboli in ApoE-/- mice显示文摘Objective: The objective of this study was to investigate the mechanisms underlying anti-embolism and extravasational effects of traditional Chinese medical prescription YiqiHuoxue(YQHX) formula in ApoE-/-mice with cerebral vascular microemboli. Materials and Methods: An ApoE-/-mice model with microemboli was developed by infusing fluorescently labeled heterologous fibrin-rich microparticles into the internal carotid artery of ApoE -/-gene knockout male mice through the common carotid artery. Before microemboli injection, the animals were randomly divided into four groups of 10 animals, treated daily for 6 weeks by intragastric administration: The ApoE-/-control group(physiological saline, 0.2 mL/10 g/d), YQHX group(0.2 ml/10 g/d), clopidogrel group(3 mg/kg/d), and atorvastatin group(3 mg/kg/d);a further group was constituted of normal male C57 BL/6 J mice(with the same genetic background as ApoE-/-mice;normal control group;no treatment;microemboli injection). The mice in each microemboli group were divided into three subgroups, the 2-h, 24-h, and 72-h subgroups, corresponding to the time after microemboli injection. Two hours(or 24 h or 72 h) after microemboli injection, the changes in aortic intima and brain tissue were analyzed by histopathology, the amounts of fluorescent emboli being measured by fluorescence microscopy image analysis. Comparison points included the microemboli induced loss of aorta functions and pathological changes, atherosclerotic plaque, brain ultrastructure and functions, and embolus extravasation. Results: Loss of aorta functions and adverse pathological changes, atherosclerotic plaque, serious damage in brain ultrastructure and functions, and reduced thrombus elimination were obviously serious in microemboli injected ApoE-/-mice. These symptoms were significantly relieved by the YQHX pretreatment:(i) the ratio of thrombus accumulation was increased with a significant decrease in thrombus extravasation in ApoE-/-mice, while YQHX induced an increased thrombus extravasation;(ii) the degree of aortic intimal thickening and brain tissue structural disorders were significantly increased in ApoE-/-mice, but overtly inhibited in the YQHX group;(iii) YQHX restored cell viability and homeostasis in the brain;(iv) YQHX regulated the expression of pro-and anti-inflammatory cytokines in the aorta;and(v) YQHX reduced cortical nerve nuclei pyknosis, edema, liquefaction, and necrosis induced by brain hypoxia, especially in the 24 h and 72 h groups. Conclusions: These findings indicate that the protective effects of YQHX on the brain against microemboli-induced injury may be attributed to the activation of extravasation mechanisms, which are involved in the cerebrovascular injury pathway and constitutively important in the progression of ischemic stroke. | Chao Jiang Ting Wang Zhong-Ju Xu Xu Chao Pierre Duez | 2020 | World Journal of Traditional Chinese Medicine2020，6，1： | 2 |
| 4 | Good practice in reviewing and publishing studies on herbal medicine, with special emphasis on traditional Chinese medicine and Chinese materia medica显示文摘 | Kelvin Chan Debbie Shaw Monique S.J. Simmonds Christine J. Leon Qihe Xu Aiping Lu Ian Sutherland Svetlana Ignatova You-Ping Zhu Rob Verpoorte Elizabeth M. Williamson Pierre Duez | 2012 | Journal of Ethnopharmacology2012，，3： | 1 |
| 5 | Pharmacovigilance of herbal medicine显示文摘 | Debbie Shaw Ladds Graeme Duez Pierre Williamson Elizabeth Chan Kelvin | 2012 | Journal of Ethnopharmacology2012，，3： | 1 |
| 6 | ETHNOBOTANICAL STUDY OF PLANTS REPUTED ANTIMALARIAL IN THE TOWN OF BAGIRA, SOUTH-KIVU IN DR CONGO显示文摘In order to identify plants and recipes used in the treatment of malaria in Bagira,an ethnobotanical study was conducted from December 2013 to February 2014,by interviewing 85 traditional healers(46.9±12.0 average age;range:19-79 years).The direct interview using a questionnaire was used to collect ethnobotanical information.A specimen of each plant was collected | Bashige Chiribagula Manya Mboni Bakari Amuri Kahumba Byanga Pierre Duez Lumbu Simbi | 2015 | World Journal of Traditional Chinese Medicine2015，1，4： | 0 |
| 7 | POLYPHENOLS, CYTOTOXICITY AND ENZYMATIC ACTIVITIES OF THREE ERIACEAE SPECIES FROM ALGERIA显示文摘In Algerian traditional medicine,Erica arborea(EA),Erica multiflora(EM)and Arbutus unedo(AU)are reported as antiseptic,diuretic,astringent,depurative,and to treat scalds and wounds.The methanolic extracts of their leaves and aerial parts were screened for enzyme inhibitory and cytotoxic activities.TLC and HPLC chromatographic profiles based on flavonoids allowed to easily distinguish between the three investigated species.Inhibitory | Guendouze-Bouchefa Nama Brahmi Fatiha Madani Khodir Chibane Mohamed Kiendrebeogo Martin Stévigny Caroline Duez Pierre | 2015 | World Journal of Traditional Chinese Medicine2015，1，4： | 0 |
| 8 | Essential tremor:A three-dimensional neurosphere in vitro model to assess the neurotoxicity of harmane显示文摘Objectives: To use a novel in vitro model of three-dimensional(3D) neurosphere cultures to assess neurotoxic or neuroprotective effects with harmane as a model compound.Methods: A reproducible model of 3D spheroids was developed from embryonic mouse cortical neurons,using molded agarose micro-wells;this method seems particularly practical as it is customizable and widely available and does not require specific cell treatments or assay components different from 2D cultures, allowing for the easy transposition of routine protocols. To assess the neurotoxic effects of harmane, a resazurin assay was performed to measure cell viability, and a highly sensitive fluorometric method, based on the oxidation of dichlorodihydrofluorescein, was applied to measure eventually induced reactive oxygen species(ROS) after exposure to harmane at increasing concentrations of 50 100,and 250 μm.Results: Hydrogel microwells facilitated the assembly of spheroids containing neurons and glial cells into a complex 3D structure and prevented the agglomeration of spheroids. Exposure to harmane induced cytotoxicity in 3D neural spheroids, which was correlated with harmane concentrations, with a 27%reduction in viability at 250 μm. Harmane that did not induce significant levels of oxidative stress was detected for all tested concentrations.Conclusion: This 3D neurosphere model mimics a neuronal microenvironment, allowing a fine study of neurodegenerative disorders and the effects of chemicals on the brain. This model opens novel opportunities, not only from a pathogenetic point of view but also from a therapeutic perspective. | Rania Aro Amandine Nachtergael Laurence Ris Mario Manto Pierre Duez | 2023 | Journal of Traditional Chinese Medical Sciences2023，10，1： | 0 |
| 9 | SURVEY OF PLANTS USED IN TRADITIONAL MEDICINE AGAINST MALARIA IN BUKAVU AND UVIRA/DR CONGO显示文摘Malaria,for its high prevalence and morbidity,is one of the major tropical parasitic diseases,particularly in DR Congo.Its therapy not only uses modem antimalarials,for which many Resistance problems are encountered,but also medicinal plants which are promising sources for new and effective antimalarials.This study was conducted | Henry Manya Mboni Valentin Bashige Chiribagula Salvius Bakari Amuri Dede Mbangu Munahu Joseph Kahumba Byanga Caroline Stévigny Jean-Baptiste Lumbu Simbi Pierre Duez | 2015 | World Journal of Traditional Chinese Medicine2015，1，4： | 0 |
| 10 | SEARCH FOR TRANSLESION SYNTHESIS MODULATORS FROM NATURAL PRODUCTS:ANALYTICAL DEVELOPMENT, VALIDATION AND APPLICATIONS显示文摘DNA translesion synthesis(TLS)is a DNA damage tolerance mechanism that relies on a series of specialized DNA polymerases able to bypass a lesion on a DNA template strand during replication or post-repair synthesis.TLS DNA polymerases pursue replication by inserting a base opposite this lesion,correctly or incorrectly | Amandine Nachtergael Déborah Lanterbecq Martine Gadenne Alexandra Belayew Pierre Duez | 2015 | World Journal of Traditional Chinese Medicine2015，1，4： | 0 |
| 11 | ROLE OF MEDICINAL AND AROMATIC PLANTS IN MODERN URBAN COMMUNITIES OF BRUSSELS显示文摘Medicinal and aromatic plants(MAPs)have been used by humans for centuries.A big part of the knowledge and interest in MAPs was lost during periods of intensive industrialisation and urbanisation.According to the estimations of the United Nations,the world urban population is expected to increase by 84%by 2050. | Vitalija Povilaityte-Petri Pierre Duez | 2015 | World Journal of Traditional Chinese Medicine2015，1，4： | 0 |
| 12 | PRELIMINARY ASSESSMENT OF THE CYTOTOXICITY AND GENOTOXICITY OF AN ASSOCIATION ARISTOLOCHIA-MAGNOLIA显示文摘Herbal medicines are widely used around the world,either for primary health care or as complementary medicines.These herbal medicines are generally considered as safe and reliable.However,herbs can sometimes cause serious health troubles.For example,the aristolochic acid nephropathy(AAN),a progressive renal interstitial | Melanie Poivre Amandine Nachtergael Alexandra Belayew Pierre Duez | 2015 | World Journal of Traditional Chinese Medicine2015，1，4： | 0 |
| 13 | Herbal Medicines for Acute Kidney Injury: Evidence, Gaps and Frontiers显示文摘Acute kidney injury(AKI) is a major health threat worldwide. The literature on herbal intervention in AKI was searched from English and Chinese databases and reports were critically analyzed in terms of preventing AKI, promoting repair and regeneration, enhancing extrarenal clearance of uremic toxins, and preventing progression to chronic kidney disease(CKD). Altogether, 16 herbal formulae and a few extracts derived from individual herbs were reported to prevent or mitigate AKI in animal models induced by renal ischemia/reperfusion, cisplastin,gentamicin, glycerol, adenine, sepsis or physical exhaustion. Four formulae and six individual herbs were reported to accelerate recovery and/or to prevent CKD in established AKI animal models. Intrarectal herbal medicines, with or without simultaneous oral administration, were reported in six clinical trials and in an animal model to increase extrarenal clearance of uremic toxins. Additional 13 clinical trials reported oral or intravenous herbal interventions in AKI of different etiologies. Despite recurring problems, notably poor compliance with good practice guidelines for clinical trials and for authentication, naming and quality control of herbal materials, accumulating experimental data on the preventive effects of herbal medicines in AKI look encouraging and urge for better, definitive trials to guide clinical practice. Herbal enemas promoting extrarenal clearance of uremic toxins seem cost-effective, but better clinical evidence is certainly needed before any affirmative recommendation be made for AKI patients without access to dialysis. New frontiers, however, lie in those herbal remedies that promote repair/regeneration and prevent chronicity after AKI. Recent experimental data suggest that this may be possible. | Valérian Bunel Fan Qu Pierre Duez Qi-he Xu | 2015 | World Journal of Traditional Chinese Medicine2015，1，3： | 0 |
| 14 | AN ORIGINAL DIAGNOSTIC SYSTEM FOR MALARIA TO SCREEN THE ANTIPLASMODIAL ACTIVITY OF AFRICAN HERBAL MEDICINES显示文摘Malaria,caused by protozoa of the genus Plasmodium,causes some 198 million of confirmed cases and about584,000 of deaths per year.Its management needs both accurate diagnostic and treatments availability.The current diagnostic techniques(rapid diagnostic tests and microscopy)appear either too expensive,lowly sensitive or difficult to implement.The hemozoin('malaria pigment') | Okusa Philippe N.Traoré Orokia Duez Pierre Avaert Richard Haddadi Zainab Blankert Bertrand Okusa Philippe | 2015 | World Journal of Traditional Chinese Medicine2015，1，4： | 0 |
| 15 | NEW STRATEGIES FOR THE CONTROL OF BACTERIAL INFECTIONS: MODULATORS OF QUORUM SENSING AND BIOFILM FORMATION FROM MALAGASY DALBERGIA SPECIES显示文摘Considering the WHO warning about the emergence of a’post-antibiotic’era during the 21st century in which common infections and minor injuries will have a dramatic impact on human death toll,search for new potential antibacterial drug targets became a necessary need.Targets that are extensively explored concern the modulation | Tsiry Rasamiravaka Pierre Duez Mondher El Jaziri | 2015 | World Journal of Traditional Chinese Medicine2015，1，4： | 0 |
| 16 | PHENOLIC PROFILE, ANTIBACTERIAL,NEUROPROTECTIVE AND CYTOTOXIC PROPERTIES OF THREE ALGERIAN MENTHA SPECIES显示文摘This study was designed to evaluate the antibacterial(direct effect and reversal of antibiotics-resistance),neuroprotective(acetylcholinesterase and carboxylesterase inhibition)and cytotoxic(MTT assay on 2 human cancer cell lines)properties of three Mentha species[M.pulegium L.(MP),M.rotundifolia(L.)Huds(MR),and M.spicata L.(MS)]harvested in Bejaia(Algeria).The phenolic | Brahmi Fatiha Guendouze Naima Madani Khodir PhillipeN.Okusa Martin Kiendrebeogo Kamagaju Léocadie Stévigny Caroline Chibane Mohamed Duez Pierre | 2015 | World Journal of Traditional Chinese Medicine2015，1，4： | 0 |
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