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| 1 | Transplantation of collagen scaffold with autologous bone marrow mononuclear cells promotes functional endometrium reconstruction via downregulating ΔNp63 expression in Asherman's syndrome显示文摘Asherman's syndrome(AS) is a common disease that presents endometrial regeneration disorder. However, little is known about its molecular features of this aregenerative endometrium in AS and how to reconstruct the functioning endometrium for the patients with AS. Here, we report that ΔNp63 is significantly upregulated in residual epithelial cells of the impaired endometrium in AS; the upregulated-ΔNp63 induces endometrial quiescence and alteration of stemness. Importantly, we demonstrate that engrafting high density of autologous bone marrow mononuclear cells(BMNCs) loaded in collagen scaffold onto the uterine lining of patients with AS downregulates ΔNp63 expression, reverses ΔNp63-induced pathological changes, normalizes the stemness alterations and restores endometrial regeneration. Finally, five patients achieved successful pregnancies and live births. Therefore, we conclude that ΔNp63 is a crucial therapeutic target for AS. This novel treatment significantly improves the outcome for the patients with severe AS. | Guangfeng Zhao Yun Cao Xianghong Zhu Xiaoqiu Tang Lijun Ding Haixiang Sun Juan Li Xinan Li Chenyan Dai Tong Ru Hui Zhu Jingjie Lu Caimei Lin Jingmei Wang Guijun Yan Huiyan Wang Lei Wang Yimin Dai Bin Wang Ruotian Li Jianwu Dai Yan Zhou Yali Hu | 2017 | Science China(Life Sciences)2017，60，4： | 39 |
| 2 | Pre-treatment with IL-1β enhances the efficacy of MSC transplantation in DSS-induced colitis显示文摘间充质的干细胞(MSC ) 试验性地被使用了对待煽动性的混乱,部分由于他们的抑制免疫力的性质。尽管 interleukin-1β(IL-1β) 是最重要的煽动性的调停人之一,成长证据显示那 IL-1β发信号得到 MSC 的抑制免疫力的性质。然而,它仍然保持不清楚怎么 IL-1β发信号完成这项活动。这里,我们集中于 IL-1β 的治疗学的功效;在葡聚糖硫酸盐钠(决策支持系统) 的告知的 MSC 劝诱了大肠炎模型,除了加重的机制。我们首先发现了那 IL-1β没有在 vitro 的任何看得见的显型变化,告知的 MSC 显著地稀释了导致决策支持系统的鼠科的大肠炎的发展。而且, IL-1β告知的 MSC 通过提高 cyclooxygenase-2 (COX-2 ) 在怒气和 mesenteric 淋巴节点(MLN ) 调制了有免疫力的房间的平衡, IL-6 和 IL-8 表示并且影响腹巨噬细胞的极化。重要地, IL-1β告知的 MSC 拥有了一个提高的能力经由 chemokine 受体类型 4 的 upregulation (CXCR4 ) 移居到内脏的煽动性的地点表示。在摘要, IL-1β告知的 MSC 在对待导致决策支持系统的大肠炎改进了功效,它部分至少取决于他们的增加的抑制免疫力的能力和提高的迁居能力。 | Hongye Fan Guangfeng Zhao Liu Liu Fei Liu Wei Gong Xianqin Liu Liu Yang Jianjun Wang Yayi Hou | 2012 | Cellular & Molecular Immunology2012，9，6： | 21 |
| 3 | Upregulation of CD81 in trophoblasts induces an imbalance of Treg/Th17 cells by promoting IL-6 expression in preeclampsia显示文摘The disturbance of maternal immune tolerance to a semiallogeneic fetus is recognized as one of the key pathologies of preeclampsia(PE),in which an imbalance between the inflammation-limiting regulatory T cells(Tregs)and the inflammationmediating Th17 cells plays an essential role.Previously,we reported that the abnormal upregulation of tetraspannin CD81 in trophoblast cells(fetal component)participated in the pathogenesis of PE.However,as one of the potential immune regulatory molecules,whether CD81 induces PE by interfering with the balance of the maternal immune system has not yet been clarified.Thus,we investigated the relationship between the upregulation of CD81 in trophoblast cells and the imbalance of Treg and Th17 cells in mothers.Here,we demonstrated that upregulation of CD81 in trophoblast cells was accompanied by a decrease in Treg cells and an increase in Th17 cells in both the basal plate(placental maternal side)and peripheral blood of patients with PE.In vitro culture of naïve T cells with medium from the CD81-overexpressing trophoblast cell line HTR-8 resulted in enhanced differentiation of T cells into Th17 cells and decreased the formation of Tregs,which was dependent on the paracrine signaling of IL-6 in trophocytes,induced by CD81.In a CD81-induced PE rat model,we found a significant shift of T cell differentiation towards Th17 cells,and administration of IL-6 antibody mitigated the PE phenotype and the imbalance of the Treg/Th17 cells.These results define a vital regulatory cascade involving trophocyte-derived CD81,IL-6,and maternal Treg/Th17 cells in the pathogenesis of PE and suggests new therapeutic approaches based on CD81 and IL-6 downregulation to prevent human PE. | Hailin Ding Yimin Dai Yi Lei Zhiyin Wang Dan Liu Ruotian Li Li Shen Ning Gu Mingming Zheng Xiangyu Zhu Guangfeng Zhao Yali Hu | 2019 | Cellular & Molecular Immunology2019，16，3： | 15 |
| 4 | Pravastatin alleviates lipopolysaccharide-induced placental TLR4 over-activation and promotes uterine arteriole remodeling without impairing rat fetal development显示文摘Preeclampsia is associated with over-activation of the innate immune system in the placenta,in which toll-like receptor 4(TLR4) plays an essential part.With their potent anti-inflammatory effects,statins have been suggested as potential prevention or treatment of preeclampsia,although evidence remains inadequate.Herewith,we investigated whether pravastatin could ameliorate preeclampsia-like phenotypes in a previously established lipopolysaccharide(LPS)-induced rat preeclampsia model,through targeting the TLR4/NF-κB pathway.The results showed that pravastatin reduced the blood pressure [maximum decline on gestational day(GD) 12,(101.33±2.49) mmHg vs.(118.3±1.37) mmHg,P<0.05] and urine protein level [maximum decline on GD9,(3,726.23± 1,572.86) μg vs.(1,991.03 ±609.37)μg,P< 0.05],which were elevated following LPS administration.Pravastatin also significantly reduced the rate of fetal growth restriction in LPS-treated rats(34.10% vs.8.99%,P<0.05).Further pathological analyses suggested a restoration of normal spiral artery remodeling in preeclampsia rats by pravastatin treatment.These effects of pravastatin were associated with decreased TLR4/NF-κB protein levels in the placenta and IL-6/MCP-1 levels in serum.Additionally,no obvious abnormalities in fetal liver,brain,and kidney were found after administration of pravastatin.These results provide supportive evidence for use of pravastatin in preventing preeclampsia. | Muyi Yang Zhenyu Diao Zhiyin Wang Guijun Yan Guangfeng Zhao Mingming Zheng Anyi Dai Yimin Dai Yali Hu | 2018 | The Journal of Biomedical Research2018，32，4： | 5 |
| 5 | Cooperative effect from cation and anion of pyridine-containing anion-based ionic liquids for catalysing CO_2 transformation at ambient conditions显示文摘Pyridine-containing anion-based ionic liquids(PA-ILs) with two kinds of interaction sites to bind CO_2, e.g., [P4444][2-OP], were found to be highly efficient for catalysing the cycloaddition reactions of atmospheric CO_2 with epoxides at room temperature under metal-and halogen-free conditions, producing a series of cyclic carbonates in high yields. It was demonstrated that the cooperative interaction from two interaction sites in the anions of PA-ILs activated CO_2, while the cation activated the epoxides substrates via coordination to the central P+ unit, thus resulting in the high activity of the IL catalysts. | Guangfeng Yuan Yanfei Zhao Yunyan Wu Ruipeng Li Yu Chen Dongmei Xu Zhimin Liu | 2017 | Science China Chemistry2017，60，7： | 2 |
| 6 | Efficacy compared between entecavir and adefovir dipivoxil on HBeAg-positive nucleos(t)ide-naive patients with chronic hepatitis B at week 12 and week 48显示文摘Objective:To evaluate the efficacy of entecavir and adefovir dipivoxil on HBeAg-positive nucleos(t)ide-naive patients with chronic hepatitis B with the method of Meta analysis.Methods:We searched PUBMED,EMBASE,CNKI (China National Knowledge Infrastructure),the Cochrane Central Register of Controlled Trials and the Cochrane Database of Systematic Reviews with reference to all data documented before May 2010.The dosage of entecavir and adefovir dipivoxil was 0.5 mg/d and 10 mg/d,respectively.Heterogeneity was examined by Chi-square test,the relative risk calculated and forest plot drawn.Rates of undetected serum HBV DNA,serum alanine aminotransferase (ALT) normalization,HBeAg clearance,HBeAg seroconversion and adverse effect occurrence were analyzed.Results:Six articles were included,which fit well in with this study.Meta analysis showed that the rate of undetected serum HBV DNA(P=0.000 2 at week 12,P=0.002 at week 48)and that of serum ALT normalization(P=0.04 at week 12,P=0.008 at week 48)in the entecavir group were higher than those in the adefovir dipivoxil group.However,no statistic significance existed between the two groups in the rate of HBeAg clearance (P=0.17),the rate of HBeAg seroconversion(P=0.53)or the rate of adverse effect occurrence(P=0.92)at week 48.Conclusion:Entecavir was superior to adefovir dipivoxil in decreasing serum HBV DNA and normalizing serum ALT in the HBeAg-positive nucleos(t)ide-naive patients with chronic hepatitis B. | Zhao Pan Xu Dongping Wang Xi Duan Guangfeng Huang Lili | 2010 | Journal of Medical Colleges of PLA(China)2010，25，5： | 2 |
| 7 | Correlation and Significance of Midkine and Estrogen Receptor Beta Protein Expression in Non-Small Cell Lung Cancer显示文摘OBJECTIVE Midkine (MK),a new member of the heparin-binding growth factor family,was found recently to have a highexpression level in many carcinoma specimens,including thoseof the esophagus,gall bladder,pancreas,colorectum,breast andlung.Estrogen receptor beta (ER-β),a recently cloned estrogenreceptor subtype,was also found to be highly expressed in lungtumor tissue,in contrast to a lower level of expression in normallung tissue.However,few relevant studies on these proteins havebeen published.The aims of our study were to investigate theexpression of midkine and ER-β proteins in non-small cell lungcancer (NSCLC) and to examine the relationship between theirexpression and the clinicopathologic data as well as to analyse thecorrelation of their expression in NSCLC.METHODS By immunohistochemistry,MK and ER-β were ex-amined in 24 surgically resected cases of NSCLC with their corre-sponding paraneoplastic and normal lung tissues.RESULTS MK and ER-β were overexpressed in NSCLC.Thelevels of MK and ER-β expression in NSCLC were found to be sig-nificantly negatively correlated with the pathological classification(P=0.042 and 0.021,respectively),and their expression decreasedwith a raise in the classification.Spearman's correlation analysisshowed that the correlation of their expression in NSCLC wasstrong (correlation coefficient[r_s]= 0.535,P=0.007<0.01).CONCLUSION The expression levels of MK and ER-β to someextent reflect the malignant degree of NSCLC,and their combineddetection may be of great value in early diagnosis,treatments ofpatients with NSCLC and can predict the prognoses. | Shihua Zhang Guangfeng Zhao Qingling Wang Kaihua Lu Yayi Hou | 2008 | Chinese Journal of Clinical Oncology2008，5，6： | 2 |
| 8 | Placenta-derived IL-32β activates neutrophils to promote preeclampsia development显示文摘Immune activation at the maternal-fetal interface is a main pathogenic factor of preeclampsia(PE).Neutrophils(PMNs)are activated in PE patients,but the mechanism and consequences of PMN activation need to be further explored.Here,we demonstrated that interleukin-32(IL-32)expression was significantly upregulated in syncytiotrophoblasts(STBs)and that IL-32β was the major isoform with increased expression in the placenta of severe PE(sPE)patients.Furthermore,the level of IL-32 expression in the placenta was correlated with its level in the serum of sPE patients,indicating that IL-32 in the serum is derived mainly from the placenta.Then,in vitro experiments showed that IL-32β could highly activate PMNs and that these IL-32β-activated PMNs were better able to adhere to endothelial cells(HUVECs)and enhance the expression of vascular cell adhesion molecule-1(VCAM-1)and intercellular cell adhesion molecule-1(ICAM-1)in HUVECs,which could be reversed by preincubation with the NADPH oxidase inhibitor VAS 2870.In addition,we showed that IL-32β mainly activated PMNs by binding to proteinase 3.Finally,IL-32β administration induced a PE-like phenotype in a pregnant mouse model.This study provides evidence of the involvement of IL-32β in the pathogenesis of PE. | Dan Liu Qiang Li Hailin Ding Guangfeng Zhao Zhiyin Wang Chenrui Cao Yimin Dai Mingming Zheng Xiangyu Zhu Qianwen Wu Ya Wang Honglei Duan Huirong Tang Xianyan Lu Yayi Hou Yali Hu | 2021 | Cellular & Molecular Immunology2021，18，4： | 2 |
| 9 | Twenty-seven continental ancestry-informative SNP analysis of bone remains to resolve a forensic case显示文摘We employed our previously developed 27-plex ancestry-informative single nucleotide polymorphism(SNP)panel to infer the ancestral components of bone remains of a possible foreign pilot found in south-western China.For ancestry assignment of this unknown individual,we first obtained the 27-SNP genotype of the individual.Then,based on a reference database of 3081 individuals from 33 populations,we calculated the match probability and likelihood ratio using the self-developed software program Forensic Intelligence.Inferred ancestral components of this individual were calculated by structure at K=3.A complete profile was obtained for the individual using our multiplexed SNP assay.The European population was within one order of magnitude of the highest likelihood.The major ancestral component of this individual was 97.6%European. | Qifan Sun Li Jiang Guangfeng Zhang Jing Liu Lei Zhao Wenting Zhao Caixia Li | 2019 | Forensic Sciences Research2019，4，4： | 1 |
| 10 | Independence of the brittle-ductile transition from the rubber partice size for impact-modified poly (vinyl chloride) 显示文摘 | ZHAO Chengzhe WU Guangfeng ZHAO Chao | 2006 | J Polym Sci Part B: Polym Phys2006，44，： | 1 |
| 11 | The influence of core–shell structured modifiers on the toughness of poly (vinyl chloride)显示文摘 | Guangfeng Wu Junfeng Zhao Haitao Shi Huixuan Zhang | 2004 | European Polymer Journal2004，，11： | 1 |
| 12 | A randomized, multi-central, controlled study of patients with hepatitis B e antigen-positive chronic hepatitis B treated by adefovir dipivoxil or adefovir dipivoxil plus bicyclol显示文摘 | Wen Xie Guangfeng Shi Hongfei Zhang Guiming Zhao Zujiang Yu Zhenwei Lang Hong Zhao Jie Yan Jun Cheng | 2012 | Hepatology International2012，，2： | 1 |
| 13 | Independence of the brittle-ductile transition from the rubber particle size for impact-modified poly ( vinyl chloride) 显示文摘 | ZHAO Chengzhe WU Guangfeng ZHOU Chao | 2006 | J Polym Sci: Polym Sci2006，44，： | 1 |
| 14 | Investigation on properties of a novel ceria–zirconia–praseodymia solid solution and its application in Pd-only three-way catalyst for gasoline engine emission control显示文摘 | Qiuyan Wang Guangfeng Li Bo Zhao Renxian Zhou | 2011 | Fuel2011，，： | 1 |
| 15 | The influence of core–shell structured modifiers on the toughness of poly (vinyl chloride)显示文摘 | Guangfeng Wu Junfeng Zhao Haitao Shi Huixuan Zhang | 2004 | European Polymer Journal2004，，11： | 1 |
| 16 | A novel way to isolate MSC s from umbilical cords显示文摘 | Liu Liu Xiaoyin Zhao Pengfei Li Guangfeng Zhao Yaping Wang Yali Hu Yayi Hou | 2012 | Eur J Immunol2012，，8： | 1 |
| 17 | Independence of the brittle-ductile transition from the rubber particle size for impact-modified poly(vinyl chloride)显示文摘 | Zhao Chengzhe Wu Guangfeng | 2006 | Journal of Polymer Science2006，44，： | 1 |
| 18 | Independence of the brittle-ductile transition from the rubber particle size for impact-modified poly(vinyl chloride) 显示文摘 | Zhao Chengzhe Wu Guangfeng Zhou Chao | 2006 | Polymer Chemistry2006，44，4： | 1 |
| 19 | The influence of core-shell structured modifiers on the toughness of poly (vinyl chloride)显示文摘 | WU Guangfeng ZHAO Junfeng SHI Haitao | 2004 | Eur Polym J2004，40，11： | 1 |
| 20 | Detection of quantitative trait loci for heading date based on the doubled haploid progeny of two elite Chinese wheat cultivars显示文摘 | Kunpu Zhang Jichun Tian Liang Zhao Bin Liu Guangfeng Chen | 2009 | Genetica2009，，3： | 1 |
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