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| 1 | Antimicrobial susceptibility testing for Helicobacter pylori in times of increasing antibiotic resistance显示文摘The gram-negative bacterium Helicobacter pylori(H.pylori)causes chronic gastritis,gastric and duodenal ulcers,gastric cancer and mucosa-associated lymphoid tissue lymphoma.Treatment is recommended in all symptomatic patients.The current treatment options for H.pylori infection are outlined in this review in light of the recent challenges in eradication success,largely due to the rapid emergence of antibiotic resistant strains of H.pylori.Antibiotic resistance is a constantly evolving process and numerous studies have shown that the prevalence of H.pylori antibiotic resistance varies significantly from country to country,and even between regions within the same country.In addition,recent data has shown that previous antibiotic use is associated with harbouring antibiotic resistant H.pylori.Local surveillance of antibiotic resistance is warranted to guide clinicians in their choice of therapy.Antimicrobial resistance is assessed by H.pylori culture and antimicrobial susceptibility testing.Recently developed molecular tests offer an attractive alternative to culture and allow for the rapid molecular genetic identification of H.pylori and resistance-associated mutations directly from biopsy samples or bacterial culture material.Accumulating evidence indicates that surveillance of antimicrobial resistance by susceptibility testing is feasible and necessary to inform clinicians in their choice of therapy for management of H.pylori infection. | Sinéad M Smith Colm O’Morain Deirdre McNamara | 2014 | World Journal of Gastroenterology2014，20，29： | 40 |
| 2 | Colorectal cancer screening显示文摘Colorectal cancer is a major public health burden worldwide. There is clear-cut evidence that screening will reduce colorectal cancer mortality and the only contentious issue is which screening tool to use. Mos evidence points towards screening with fecal occul blood testing. The immunochemical fecal occult blood tests have a higher sensitivity than the guaiac-based tests. In addition, their automation and haemoglobin quantification allows a threshold for colonoscopy to be selected that can be accommodated within individua health care systems. | Ramona M McLoughlin Colm A O'Morain | 2006 | World Journal of Gastroenterology2006，12，42： | 8 |
| 3 | Molecular detection of Helicobacter pylori antibiotic resistance in stool vs biopsy samples显示文摘AIM To compare(1) demographics in urea breath test(UBT) vs endoscopy patients; and(2) the molecular detection of antibiotic resistance in stool vs biopsy samples.METHODS Six hundred and sixteen adult patients undergoing endoscopy or a UBT were prospectively recruited to the study. The Geno Type Helico DR assay was used to detect Helicobacter pylori(H. pylori) and antibiotic resistance using biopsy and/or stool samples from CLOpositive endoscopy patients and stool samples from UBT-positive patients. RESULTS Infection rates were significantly higher in patients referred for a UBT than endoscopy(overall rates: 33% vs 19%; treatment-na?ve patients: 33% vs 14.7%, respectively). H. pylori-infected UBT patients were younger than H. pylori-infected endoscopy patients(41.4 vs 48.4 years, respectively, P < 0.005), with a higher percentage of H. pylori-infected males in the endoscopy-compared to the UBT-cohort(52.6% vs 33.3%, P = 0.03). The Geno Type Helico DR assay was more accurate at detecting H. pylori infection using biopsy samples than stool samples [98.2%(n = 54/55) vs 80.3%(n =53/66), P < 0.005]. Subset analysis using stool and biopsy samples from CLO-positive endoscopy patients revealed a higher detection rate ofresistance-associated mutations using stool samples compared to biopsies. The concordance rates between stool and biopsy samples for the detection of H. pylori DNA, clarithromycin and fluoroquinolone resistance were just 85%, 53% and 35%, respectively. CONCLUSION Differences between endoscopy and UBT patients provide a rationale for non-invasive detection of H. pylori antibiotic resistance. However, the Geno Type Helico DR assay is an unsuitable approach. | Denise E Brennan Joseph Omorogbe Mary Hussey Donal Tighe Grainne Holleran Colm O'Morain Sinéad M Smith Deirdre McNamara | 2016 | World Journal of Gastroenterology2016，22，41： | 6 |
| 4 | Can bacterial virulence factors predict antibiotic resistant Helicobacter pylori infection?显示文摘AIM To evaluate the association between virulence factor status and antibiotic resistance in Helicobacter pylori(H. pylori)-infected patients in Ireland. METHODS DNA was extracted from antral and corpus biopsies obtained from 165 H. pylori-infected patients. Genotyping for clarithromycin and fluoroquinolone-mediating mutations was performed using the Genotype Helico DR assay. cag A and vac A genotypes were investigated using PCR. RESULTS Primary, secondary and overall resistance rates for clarithromycin were 50.5%(n = 53/105), 78.3%(n = 47/60) and 60.6%(n = 100/165), respectively. Primary, secondary and overall resistance rates for fluoroquinolones were 15.2%(n = 16/105) and 28.3%(n = 17/60) and 20%(n = 33/165), respectively. Resistance to both antibiotics was 12.4%(n = 13/105) in treatment-na?ve patients, 25%(n = 15/60) in those previously treated and 17%(n = 28/165) overall. A cag A-positive genotype was detected in 22.4%(n = 37/165) of patient samples. The dominant vac A genotype was S1/M2 at 44.8%(n = 74/165), followed by S2/M2 at 26.7%(n = 44/165), S1/M1 at 23.6%(n = 39/165) and S2/M1 at 4.8%(n = 8/165). Primary clarithromycin resistance was significantly lower in cag A-positive strains than in cag A-negative strains [32%(n = 8/25) vs 56.3%(n = 45/80); P = 0.03]. Similarly, in patients infected with more virulent H. pylori strains bearing the vac A s1 genotype, primary clarithromycin resistance was significantly lower than in those infected with less virulent strains bearing the vac A s2 genotype, [41%(n = 32/78) vs 77.8%(n = 21/27); P = 0.0001]. No statistically significant association was found between primary fluoroquinolone resistance and virulence factor status.CONCLUSION Genotypic H. pylori clarithromycin resistance is high and cag A-negative strains are dominant in our population. Less virulent(cag A-negative and vac A S2-containing) strains of H. pylori are associated with primary clarithromycin resistance. | Denise E Brennan Colin Dowd Colm O'Morain Deirdre McNamara Sinéad M Smith | 2018 | World Journal of Gastroenterology2018，24，9： | 4 |
| 5 | Recent technological advances for the determination of food authentieity显示文摘 | Linda M Reid Colm P O Donnell Gerard Downey | 2006 | Trends in Food Science & Technology2006，17，7： | 1 |
| 6 | Preliminary studies for the differentiation of apple juice samples by chemometric analysis of solid-phase microextraction-gas chromatographic data显示文摘 | Reid L M Colm P 0 D Kelly J D | 2004 | Journal of Agricultural and Food Chemistry2004，52，23： | 1 |
| 7 | Maintaining Healthy Population Diversity Using Adaptive Crossover, Mutation, and Selection 显示文摘 | BRUAN M G JOHN M COLM O | 2011 | IEEE Transactions on Evolutionary Computation2011，15，5： | 1 |
| 8 | Photoemission study of onion like quantum dot quantum well and double quantum well nanocrystals of CdS and HgS显示文摘 | Holger B Dirk D Colm M | 2003 | J Phys Chem2003，107，： | 1 |
| 9 | Long-term (1243 days), low-temperature (4-15 C), anaerobic biotreatment of acidified wastewaters: Bioprocess performance and physiological characteristics显示文摘 | Rory M McKeown Colm S | 2009 | Water Research2009，43，6： | 1 |
| 10 | Recent technological advances for the determination of food authenticity显示文摘 | Linda M Reida Colm P O Donnellb Gerard Downey | 2006 | Trends in Food Science & Technology2006，17，： | 1 |
| 11 | Large pore diameter MCM-41 and its ap-plication for lead removal from aqueous media 显示文摘 | Salah A Idris? Christine M Davidson Colm McMana-mon | 2011 | Journal of Hazardous Materials2011，，185： | 1 |
| 12 | Sequential Therapy for Helicobacter pylori Eradication: A Critical Review显示文摘 | Javier P. Gisbert Xavier Calvet Anthony O?Connor Francis Mégraud Colm A. O?Morain | 2010 | Journal of Clinical Gastroenterology2010，，5： | 1 |
| 13 | An artificial neural network model for toughness properties in microalloyed steel in consideration of industrial production conditions显示文摘 | ColM Ertunc H M Yilmaz M | 2007 | Materials and Design2007，28，： | 1 |
| 14 | Use of low-dose gabapen-tin for aggressive behavior in vascular and mixed vascular/ Alzheimer dementia显示文摘 | Colm C Sinead M Hiberet T | 2013 | J Neuropsychiatry Clinical Neuroscienc-es2013，25，2： | 1 |
| 15 | Sequential Therapy for Helicobacter pylori Eradication: A Critical Review显示文摘 | Javier P. Gisbert Xavier Calvet Anthony O?Connor Francis Mégraud Colm A. O?Morain | 2010 | Journal of Clinical Gastroenterology2010，，5： | 1 |
| 16 | Lymphatic metastases from pelvic tumors: anatomic classification,characterization and staging 显示文摘 | McMahon Colm J Rofsky Neil M Pedrosa Ivan | 2010 | Radiology2010，254，1： | 1 |
| 17 | Earcy experience with the Nuss minimally invasive correction of pectus excavatum in adults显示文摘 | Colm D Cunning T Ramsay M | 2002 | World J Surg2002，26，10： | 1 |
| 18 | Econometric-model of television ownership显示文摘 | COLM M JUNE R | 1976 | Economic and social review1976，7，3： | 1 |
| 19 | Diagnosis of Helicobacter pylori infection with a new non-in vasive antigen-based assay显示文摘 | Dino Vaira Peter Malfertheiner Francis Megraud Anthony TR Axon Michel Deltenre Alexander M Hirschl Giovanni Gasbarrini Colm O’Morain Jose M Pajares Garcia Mario Quina Guido NJ Tytgat | 1999 | The Lancet1999，，9172： | 1 |
| 20 | Combined antrum and corpus biopsy protocol improves Helicobacter pylori culture success显示文摘BACKGROUND Helicobacter pylori(H.pylori)causes chronic gastritis,peptic ulcer disease,gastric adenocarcinoma and mucosa-associated lymphoid tissue lymphoma.Eradication rates have fallen,mainly due to antimicrobial resistance.Consensus guidelines recommend that first-line treatment is based on the local prevalence of antimicrobial resistance and that rescue therapies are guided by antimicrobial susceptibility testing(AST).However,H.pylori culture is challenging and culture-based AST is not routinely performed in the majority of hospitals.Optimisation of H.pylori culture from clinical specimens will enable more widespread AST to determine the most appropriate antimicrobials for H.pylori eradication.AIM To determine whether dual antrum and corpus biopsy sampling is superior to single antrum biopsy sampling for H.pylori culture.METHODS The study received ethical approval from the joint research ethics committee of Tallaght University Hospital and St.James’s Hospital.Patients referred for upper gastrointestinal endoscopy were invited to participate.Biopsies were collected in tubes containing Dent’s transport medium and patient demographics were recorded.Biopsies were used to inoculate Colombia blood agar plates.Plates were incubated under microaerobic conditions and evaluated for the presence of H.pylori.Statistical analyses were performed using Graphpad PRISM.Continuous variables were compared using the two-tailed independent t-test.Categorical variables were compared using the two-tailed Fisher exact test.In all cases,a P value less than 0.05 was considered significant.RESULTS In all,samples from 219 H.pylori-infected patients were analysed in the study.The mean age of recruited patients was 48±14.9 years and 50.7%(n=111)were male.The most common endoscopic finding was gastritis(58.9%;n=129).Gastric ulcer was diagnosed in 4.6%(n=10)of patients,while duodenal ulcer was diagnosed in 2.7%(n=6).Single antrum biopsies were collected from 73 patients,whereas combined antrum and corpus biopsies were collected from 146 patients.There was no significant difference in age,sex or endoscopic findings between the two groups.H.pylori was successfully cultured in a significantly higher number of cases when combined antrum and corpus biopsies were used compared to a single antrum biopsy[64.4%(n=94/146)vs 49.3%(36/73);P=0.04)].CONCLUSION Combined corpus and antrum biopsy sampling improves H.pylori culture success compared to single antrum biopsy sampling. | Denise E Brennan Colm O'Morain Deirdre McNamara Sinead M Smith | 2022 | World Journal of Gastrointestinal Pathophysiology2022，13，1： | 1 |
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