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| 1 | Real applications of quantum communications in China显示文摘The first metropolitan quantum cryptography network for government administration, which is named 'Q-Government', has recently been field tested in Wuhu, Anhui Province by researchers of Key Laboratory | LI ChengZu | 2009 | Chinese Science Bulletin2009，54，17： | 16 |
| 2 | Generation of a Hutchinson-Gilford progeria syndrome monkey model by base editing显示文摘Many human genetic diseases,including Hutchinson-Gilford progeria syndrome(HGPS),are caused by single point mutations.HGPS is a rare disorder that causes premature aging and is usually caused by a de novo point mutation in the LMNA gene.Base editors(BEs)composed of a cytidine deaminase fused to CRISPR/Cas9 nickase are highly efficient at inducing C to T base conversions in a programmable manner and can be used to generate animal disease models with single amino-acid substitutions.Here,we generated the first HGPS monkey model by delivering a BE mRNA and guide RNA(gRNA)targeting the LMNA gene via microinjection into monkey zygotes.Five out of six newborn monkeys carried the mutation specifically at the target site.HGPS monkeys expressed the toxic form of lamin A,progerin,and recapitulated the typical HGPS phenotypes including growth retardation,bone alterations,and vascular abnormalities.Thus,this monkey model genetically and clinically mimics HGPS in humans,demonstrating that the BE system can efficiently and accurately generate patient-specific disease models in non-human primates. | Fang Wang Weiqi Zhang Qiaoyan Yang Yu Kang Yanling Fan Jingkuan Wei Zunpeng Liu Shaoxing Dai Hao Li Zifan Li Lizhu Xu Chu Chu Jing Qu Chenyang Si Weizhi Ji Guang-Hui Liu Chengzu Long Yuyu Niu | 2020 | Protein & Cell2020，11，11： | 11 |
| 3 | Micro/nano-structured TiO2 surface with dual-functional antibacterial effects for biomedical applications显示文摘Implant-associated infections are generally difficult to cure owing to the bacterial antibiotic resistance which is attributed to the widespread usage of antibiotics.Given the global threat and increasing influence of antibiotic resistance,there is an urgent demand to explore novel antibacterial strategies other than using antibiotics.Recently,using a certain surface topography to provide a more persistent antibacterial solution attracts more and more attention.However,the clinical application of biomimetic nano-pillar array is not satisfactory,mainly because its antibacterial ability against Gram-positive strain is not good enough.Thus,the pillar array should be equipped with other antibacterial agents to fulfill the bacteriostatic and bactericidal requirements of clinical application.Here,we designed a novel model substrate which was a combination of periodic micro/nano-pillar array and TiO2 for basically understanding the topographical bacteriostatic effects of periodic micro/nano-pillar array and the photocatalytic bactericidal activity of TiO2.Such innovation may potentially exert the synergistic effects by integrating the persistent topographical antibacterial activity and the non-invasive X-ray induced photocatalytic antibacterial property of TiO2 to combat against antibiotic-resistant implant-associated infections.First,to separately verify the topographical antibacterial activity of TiO2 periodic micro/nano-pillar array,we systematically investigated its effects on bacterial adhesion,growth,proliferation,and viability in the dark without involving the photocatalysis of TiO2.The pillar array with sub-micron motif size can significantly inhibit the adhesion,growth,and proliferation of Staphylococcus aureus(S.aureus)and Escherichia coli(E.coli).Such antibacterial ability is mainly attributed to a spatial confinement size-effect and limited contact area availability generated by the special topography of pillar array.Moreover,the pillar array is not lethal to S.aureus and E.coli in 24 h.Then,the X-ray induced photocatalytic antibacterial property of TiO2 periodic micro/nano-pillar array in vitro and in vivo will be systematically studied in a future work.This study could shed light on the direction of surface topography design for future medical implants to combat against antibiotic-resistant implant-associated infections without using antibiotics. | Xiang Ge Chengzu Ren Yonghui Ding Guang Chen Xiong Lu Kefeng Wang Fuzeng Ren Meng Yang Zhuochen Wang Junlan Li Xinxin An Bao Qian Yang Leng | 2019 | Bioactive Materials2019，4，1： | 8 |
| 4 | Effect of cooling strategies on performance and mechanism of helical milling of CFRP/Ti-6Al-4V stacks显示文摘Hole-making for Carbon Fiber Reinforced Plastics(CFRP)/Ti-6Al-4V stacks is crucial for the assembling strength of aircraft structure parts.This work carried out experimental work for helical milling(HM)of the stacks with sustainable cooling/lubrication(dry,MQL and cryogenic)conditions.Cutting forces and temperatures at the CFRP layer,Ti-6Al-4V layer and the interface of stacks were obtained by a developed measuring system.The temperatures in CFRP machining at cryogenic condition varied from-167℃to-94℃,which were much lower than those at dry and MQL conditions.The maximum temperature near the interface of stacks for the ninth hole was higher than 240℃due to heat conduction from Ti-6Al-4V layer.The hole quality,hole diameter and tool wear mechanism at different cooling/lubrication conditions were presented and discussed.MQL condition generated mainly extrusion fracture for the fibers,due to the reduced friction effect compared with dry condition.MQL was helpful to reduce the feed mark at the hole surface of Ti-6Al-4V alloy.The flank wear of cutting edge at MQL condition was better than those at dry and cryogenic conditions.Cryogenic cooling contributed to better CFRP surface with smaller delamination and hole entrance damage due to the increased resin strength and fiber brittleness.The damage near the entrance of CFRP were analyzed by the contact state of cutting edges and fibers.Additionally,hole diameters near the exit of CFPR layer were larger than other test positions.This work provided feasible processes for improving hole quality and tool life in hole-making of CFRP/Ti-6Al-4V stacks. | Jiaying GE Guang CHEN Yongxiang SU Yunhe ZOU Chengzu REN Xuda QIN Guofeng WANG | 2022 | Chinese Journal of Aeronautics2022，35，2： | 4 |
| 5 | Constitutive Modeling for Ti-6Al-4V Alloy Machining Based on the SHPB Tests and Simulation显示文摘A constitutive model is critical for the prediction accuracy of a metal cutting simulation. The highest strain rate involved in the cutting process can be in the range of 10~4–10~6 s^(–1). Flow stresses at high strain rates are close to that of cutting are difficult to test via experiments. Split Hopkinson compression bar(SHPB) technology is used to study the deformation behavior of Ti-6Al-4V alloy at strain rates of 10^(–4)–10~4s^(–1). The Johnson Cook(JC) model was applied to characterize the flow stresses of the SHPB tests at various conditions. The parameters of the JC model are optimized by using a genetic algorithm technology. The JC plastic model and the energy density-based ductile failure criteria are adopted in the proposed SHPB finite element simulation model. The simulated flow stresses and the failure characteristics, such as the cracks along the adiabatic shear bands agree well with the experimental results. Afterwards, the SHPB simulation is used to simulate higher strain rate(approximately 3×10~4 s^(–1)) conditions by minimizing the size of the specimen. The JC model parameters covering higher strain rate conditions which are close to the deformation condition in cutting were calculated based on the flow stresses obtained by using the SHPB tests(10^(–4)–10~4 s^(–1)) and simulation(up to 3×10~4 s^(–1)). The cutting simulation using the constitutive parameters is validated by the measured forces and chip morphology. The constitutive model and parameters for high strain rate conditions that are identical to those of cutting were obtained based on the SHPB tests and simulation. | CHEN Guang KE Zhihong REN Chengzu LI Jun | 2016 | Chinese Journal of Mechanical Engineering2016，29，5： | 3 |
| 6 | Increased production of recombinant prourokinase with porous microcarrier cell culture by periodic pressure oscillation in a stirred tank reactor显示文摘 | 胡显文 Gao Lihua Li Zuohu Xiao Chengzu Xu Zhaoping | 2006 | High Technology Letters2006，12，3： | 3 |
| 7 | Efficacy of Cigu Xiaozhi pill(慈菇消脂丸) on non-alcoholic steatohepatitis-associated lipoapoptosis through stress-activated c-Jun N-terminal kinase signalling pathway显示文摘OBJECTIVE: To investigate the efficacy of Cigu Xiaozhi pill(慈菇消脂丸, CGXZ) on non-alcoholic steatohepatitis(NASH)-associated lipoapoptosis through the stress-activated c-Jun N-terminal kinase(JNK)/stress-activated protein kinase signalling pathway.METHODS: Sixty male Sprague-Dawley rats were randomly divided into the following groups(10 rats each): blank control, model, low-dose CGXZ,medium-dose CGXZ, high-dose CGXZ, and positive control(treated with SP600125, a JNK inhibitor).The NASH model was established and the histomor-phological characteristics of haematoxylin and eosin-stained liver tissues were examined under a light microscope. Cell apoptosis in liver tissues was assessed via terminal deoxynucleotidyl transferase d UTP nick-end labelling assay. In addition, the m RNA and protein expression levels of p-JNK,p-c-Jun, caspase-8, Fas, and Fas-L were determined via fluorescence-based quantitative real-time PCR,immunohistochemical and Western blot assays.RESULTS: Histopathological examination of the liver showed that the model rats had moderate-to-severe steatosis with infiltration of inflammatory cells as well as significantly higher expression levels of the p-JNK, p-c-Jun, caspase-8, Fas, and Fas-L proteins, compared with those in the blank control group(P < 0.01). Hepatic lobules of the rats in the treatment groups showed significantly reduced vacuolar degeneration and steatosis as well as alleviated inflammatory cell infiltration. The high and medium-dose CGXZ groups exhibited significantly lower m RNA and protein expression levels of p-JNK, p-c-Jun, caspase-8, Fas, and Fas-L, compared with those in the model group(P < 0.05 or P <0.01).CONCLUSION: CGXZ pill inhibited the onset of hepatocyte apoptosis by regulating the expression of p-JNK, p-c-Jun, caspase-8, Fas, and Fas-L, thereby exerting therapeutic effects against NASH. | MA Yanhua YU Chengzu WU Yan HAN Tao YANG Shaojun SHI Xia | 2021 | Journal of Traditional Chinese Medicine2021，41，1： | 2 |
| 8 | God does not play dice, and neither does CRISPR/Cas9显示文摘The current dogma is that Cas9-generated double-strand breaks repaired in a template-free manner lead to imprecise insertion/deletion(indel)mutations;and that only template-mediated homology-directed repair(HDR)can facilitate precise modification.Hence,the repair products of non-homologous end joining(NHEJ)are considered heterogeneous in their outcome,and generally dismissed as random by nature.With this understanding of NHEJ it has long been used only for imprecise gene disruption;rather than deployed as a precise and predictable means for genome editing. | Chengzu Long | 2019 | National Science Review2019，6，3： | 2 |
| 9 | Prediction of cutting forces in helical milling process显示文摘 | Haiyan Wang Xuda Qin Chengzu Ren Qi Wang | 2012 | The International Journal of Advanced Manufacturing Technology2012，，9： | 2 |
| 10 | Application of genetic algorithms for optimizing the Johnson:Cook constitutive model parameters when simulating the titanium alloy Ti-6A1-4V machining process显示文摘 | CHEN Guang REN Chengzu YU Wei | 2012 | Proceedings of the Institution of Mechanical Engineers Part B: Journal of Engineering Manufacture2012，226，8： | 1 |
| 11 | Finite element simulation of high-speed machining of titanium alloy(Ti-6Al-4V) based on ductile failure model显示文摘 | Chen Guang Ren Chengzu Yang Xiaoyong | 2011 | The International Journal of Advanced Manufacturing Technology2011，225，6： | 1 |
| 12 | Measurement and finite element simulation of micro-cutting temperatures of tool tip and workpiece显示文摘 | CHEN Guang REN Chengzu ZHANG Pan | 2013 | International Journal of Machine Tools and Manufacture2013，75，： | 1 |
| 13 | An accurate method for calculating the contact subsurface stress field of hybrid ceramic ball bearing显示文摘 | WANG Cheng YU Wei REN Chengzu | 2011 | Application of Diamond and Related Materials2011，175，： | 1 |
| 14 | Pilot production of u-PA with porous microcarrier cell culture显示文摘 | Xianwen Hu Chengzu Xiao Zicai Huang Zhixia Guo Zhengguang Zhang Zuohu Li | 2000 | Cytotechnology (-)2000，，1： | 1 |
| 15 | High density and scale-up cultivation of recombinant CHO cell line and hybridomas with porous microcarrier Cytopore显示文摘 | Chengzu Xiao Zicai Huang Wengqing Li Xianwen Hu Wenlu Qu Lihua Gao Gaoyan Liu | 1999 | Cytotechnology (-)1999，，1： | 1 |
| 16 | Pilot production of u-PA with porous microcarrier cell culture显示文摘 | Hu Xianwen Xiao Chengzu Li Zuohu | 2000 | Cytotechnology2000，33，13： | 1 |
| 17 | Ratios of regulatory T cells/T‐helper 17 cells and transforming growth factor‐β1/interleukin‐17 to be associated with the development of hepatitis B virus‐associated liver cirrhosis显示文摘 | Xueping Yu Ruyi Guo Desong Ming Milong Su Chengzu Lin Yong Deng Zhenzhong Lin Zhijun Su | 2014 | J Gastroenterol Hepatol2014，，5： | 1 |
| 18 | Finite ele- ment simulation of high-speed machining of titanium alloy (Ti6A14V) based on ductile failure model 显示文摘 | Guang Chen Chengzu Ren Xiaoyong Yang | 2011 | Int J Adv Manuf Technol2011，56，： | 1 |
| 19 | Experimental Study of ELID Grinding Based on the Active Con- trol of Oxide Layer显示文摘 | Yang Lijian Ren Chengzu Jin Xinmin | 2010 | Journal of Materials Pro eessing Teehnology2010，210，： | 1 |
| 20 | Probabilistic teleporta- tion of an arbitrary two-particle state by a partially entangledthree-particle GHZ state and W state 显示文摘 | Dai Hongyi Chen Pingxing Li Chengzu | 2004 | Opt Commmun2004，231，： | 1 |
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