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| 1 | Real applications of quantum communications in China显示文摘The first metropolitan quantum cryptography network for government administration, which is named 'Q-Government', has recently been field tested in Wuhu, Anhui Province by researchers of Key Laboratory | LI ChengZu | 2009 | Chinese Science Bulletin2009，54，17： | 16 |
| 2 | Generation of a Hutchinson-Gilford progeria syndrome monkey model by base editing显示文摘Many human genetic diseases,including Hutchinson-Gilford progeria syndrome(HGPS),are caused by single point mutations.HGPS is a rare disorder that causes premature aging and is usually caused by a de novo point mutation in the LMNA gene.Base editors(BEs)composed of a cytidine deaminase fused to CRISPR/Cas9 nickase are highly efficient at inducing C to T base conversions in a programmable manner and can be used to generate animal disease models with single amino-acid substitutions.Here,we generated the first HGPS monkey model by delivering a BE mRNA and guide RNA(gRNA)targeting the LMNA gene via microinjection into monkey zygotes.Five out of six newborn monkeys carried the mutation specifically at the target site.HGPS monkeys expressed the toxic form of lamin A,progerin,and recapitulated the typical HGPS phenotypes including growth retardation,bone alterations,and vascular abnormalities.Thus,this monkey model genetically and clinically mimics HGPS in humans,demonstrating that the BE system can efficiently and accurately generate patient-specific disease models in non-human primates. | Fang Wang Weiqi Zhang Qiaoyan Yang Yu Kang Yanling Fan Jingkuan Wei Zunpeng Liu Shaoxing Dai Hao Li Zifan Li Lizhu Xu Chu Chu Jing Qu Chenyang Si Weizhi Ji Guang-Hui Liu Chengzu Long Yuyu Niu | 2020 | Protein & Cell2020，11，11： | 11 |
| 3 | Micro/nano-structured TiO2 surface with dual-functional antibacterial effects for biomedical applications显示文摘Implant-associated infections are generally difficult to cure owing to the bacterial antibiotic resistance which is attributed to the widespread usage of antibiotics.Given the global threat and increasing influence of antibiotic resistance,there is an urgent demand to explore novel antibacterial strategies other than using antibiotics.Recently,using a certain surface topography to provide a more persistent antibacterial solution attracts more and more attention.However,the clinical application of biomimetic nano-pillar array is not satisfactory,mainly because its antibacterial ability against Gram-positive strain is not good enough.Thus,the pillar array should be equipped with other antibacterial agents to fulfill the bacteriostatic and bactericidal requirements of clinical application.Here,we designed a novel model substrate which was a combination of periodic micro/nano-pillar array and TiO2 for basically understanding the topographical bacteriostatic effects of periodic micro/nano-pillar array and the photocatalytic bactericidal activity of TiO2.Such innovation may potentially exert the synergistic effects by integrating the persistent topographical antibacterial activity and the non-invasive X-ray induced photocatalytic antibacterial property of TiO2 to combat against antibiotic-resistant implant-associated infections.First,to separately verify the topographical antibacterial activity of TiO2 periodic micro/nano-pillar array,we systematically investigated its effects on bacterial adhesion,growth,proliferation,and viability in the dark without involving the photocatalysis of TiO2.The pillar array with sub-micron motif size can significantly inhibit the adhesion,growth,and proliferation of Staphylococcus aureus(S.aureus)and Escherichia coli(E.coli).Such antibacterial ability is mainly attributed to a spatial confinement size-effect and limited contact area availability generated by the special topography of pillar array.Moreover,the pillar array is not lethal to S.aureus and E.coli in 24 h.Then,the X-ray induced photocatalytic antibacterial property of TiO2 periodic micro/nano-pillar array in vitro and in vivo will be systematically studied in a future work.This study could shed light on the direction of surface topography design for future medical implants to combat against antibiotic-resistant implant-associated infections without using antibiotics. | Xiang Ge Chengzu Ren Yonghui Ding Guang Chen Xiong Lu Kefeng Wang Fuzeng Ren Meng Yang Zhuochen Wang Junlan Li Xinxin An Bao Qian Yang Leng | 2019 | Bioactive Materials2019，4，1： | 8 |
| 4 | Constitutive Modeling for Ti-6Al-4V Alloy Machining Based on the SHPB Tests and Simulation显示文摘A constitutive model is critical for the prediction accuracy of a metal cutting simulation. The highest strain rate involved in the cutting process can be in the range of 10~4–10~6 s^(–1). Flow stresses at high strain rates are close to that of cutting are difficult to test via experiments. Split Hopkinson compression bar(SHPB) technology is used to study the deformation behavior of Ti-6Al-4V alloy at strain rates of 10^(–4)–10~4s^(–1). The Johnson Cook(JC) model was applied to characterize the flow stresses of the SHPB tests at various conditions. The parameters of the JC model are optimized by using a genetic algorithm technology. The JC plastic model and the energy density-based ductile failure criteria are adopted in the proposed SHPB finite element simulation model. The simulated flow stresses and the failure characteristics, such as the cracks along the adiabatic shear bands agree well with the experimental results. Afterwards, the SHPB simulation is used to simulate higher strain rate(approximately 3×10~4 s^(–1)) conditions by minimizing the size of the specimen. The JC model parameters covering higher strain rate conditions which are close to the deformation condition in cutting were calculated based on the flow stresses obtained by using the SHPB tests(10^(–4)–10~4 s^(–1)) and simulation(up to 3×10~4 s^(–1)). The cutting simulation using the constitutive parameters is validated by the measured forces and chip morphology. The constitutive model and parameters for high strain rate conditions that are identical to those of cutting were obtained based on the SHPB tests and simulation. | CHEN Guang KE Zhihong REN Chengzu LI Jun | 2016 | Chinese Journal of Mechanical Engineering2016，29，5： | 3 |
| 5 | Increased production of recombinant prourokinase with porous microcarrier cell culture by periodic pressure oscillation in a stirred tank reactor显示文摘 | 胡显文 Gao Lihua Li Zuohu Xiao Chengzu Xu Zhaoping | 2006 | High Technology Letters2006，12，3： | 3 |
| 6 | Pilot production of u-PA with porous microcarrier cell culture显示文摘 | Xianwen Hu Chengzu Xiao Zicai Huang Zhixia Guo Zhengguang Zhang Zuohu Li | 2000 | Cytotechnology (-)2000，，1： | 1 |
| 7 | High density and scale-up cultivation of recombinant CHO cell line and hybridomas with porous microcarrier Cytopore显示文摘 | Chengzu Xiao Zicai Huang Wengqing Li Xianwen Hu Wenlu Qu Lihua Gao Gaoyan Liu | 1999 | Cytotechnology (-)1999，，1： | 1 |
| 8 | Pilot production of u-PA with porous microcarrier cell culture显示文摘 | Hu Xianwen Xiao Chengzu Li Zuohu | 2000 | Cytotechnology2000，33，13： | 1 |
| 9 | Probabilistic teleporta- tion of an arbitrary two-particle state by a partially entangledthree-particle GHZ state and W state 显示文摘 | Dai Hongyi Chen Pingxing Li Chengzu | 2004 | Opt Commmun2004，231，： | 1 |
| 10 | High density and scale-up cultivation of recombinant CHO cell line and hybridomas with porous microcarrier Cytopore显示文摘 | Chengzu Xiao Zicai Huang Wengqing Li Xianwen Hu Wenlu Qu Lihua Gao Gaoyan Liu | 1999 | Cytotechnology (-)1999，，1： | 1 |
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