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| 1 | Humoral immune response to circulating SARS-CoV-2 variants elicited by inactivated and RBD-subunit vaccines显示文摘SARS-CoV-2 variants could induce immune escape by mutations on the receptor-binding domain(RBD)and N-terminal domain(NTD).Here we report the humoral immune response to circulating SARS-CoV-2 variants,such as 501Y.V2(B.1.351),of the plasma and neutralizing antibodies(NAbs)elicited by CoronaVac(inactivated vaccine),ZF2001(RBD-subunit vaccine)and natural infection.Among 86 potent NAbs identified by high-throughput single-cell VDJ sequencing of peripheral blood mononuclear cells from vaccinees and convalescents,near half anti-RBD NAbs showed major neutralization reductions against the K417N/E484K/N501Y mutation combination,with E484K being the dominant cause.VH3-53/VH3-66 recurrent antibodies respond differently to RBD variants,and K417N compromises the majority of neutralizing activity through reduced polar contacts with complementarity determining regions.In contrast,the 242–244 deletion(242–244Δ)would abolish most neutralization activity of anti-NTD NAbs by interrupting the conformation of NTD antigenic supersite,indicating a much less diversity of anti-NTD NAbs than anti-RBD NAbs.Plasma of convalescents and CoronaVac vaccinees displayed comparable neutralization reductions against pseudo-and authentic 501Y.V2 variants,mainly caused by E484K/N501Y and 242–244Δ,with the effects being additive.Importantly,RBD-subunit vaccinees exhibit markedly higher tolerance to 501Y.V2 than convalescents,since the elicited anti-RBD NAbs display a high diversity and are unaffected by NTD mutations.Moreover,an extended gap between the third and second doses of ZF2001 leads to better neutralizing activity and tolerance to 501Y.V2 than the standard three-dose administration.Together,these results suggest that the deployment of RBD-vaccines,through a third-dose boost,may be ideal for combating SARS-CoV-2 variants when necessary,especially for those carrying mutations that disrupt the NTD supersite. | Yunlong Cao Ayijiang Yisimayi Yali Bai Weijin Huang Xiaofeng Li Zhiying Zhang Tianjiao Yuan Ran An Jing Wang Tianhe Xiao Shuo Du Wenping Ma Liyang Song Yongzheng Li Xiang Li Weiliang Song Jiajing Wu Shuo Liu Xuemei Li Yonghong Zhang Bin Su Xianghua Guo Yangyang Wei Chuanping Gao Nana Zhang Yifei Zhang Yang Dou Xiaoyu Xu Rui Shi Bai Lu Ronghua Jin Yingmin Ma Chengfeng Qin Youchun Wang Yingmei Feng Junyu Xiao Xiaoliang Sunney Xie | 2021 | Cell Research2021，31，7： | 12 |
| 2 | Crystalline behaviors and phase transition during the manufacture of fine denier PA6 fibers显示文摘Recently we have successfully produced fine denier PA6 fibers by using additives containing lanthanide compounds.Meanwhile,crystallization and phase transition of PA6 fibers during spinning and drawing processes were investigated.During the spinning process,β phase crystal could be obtained in as-spun PA6 fibers which were produced with relatively high melt draw ratio,while γ phase crystal predominated when the melt draw ratio was relatively low.β phase crystal,whose behaviors are similar with those of γ phase by FT-IR and XRD characterization,could be transformed to α form easily when PA6 fibers are immersed in boiling water.However,γ phase crystal of PA6 remains unchanged in boiling water.Thus,β and γ phase crystals of PA6 can be differentiated by the crystalline behaviors of PA6 fibers after treatment in boiling water.Further experiments demonstrate that the β phase can also be produced during a drawing process where a phase transformation from γ to α occurs.In other words,β phase may act as an intermediate state during the phase transformation. | ZHANG ChengFeng LIU YuHai LIU ShaoXuan LI HuiZhen HUANG Kun PAN QingHua HUA XiaoHui HAO ChaoWei MA QingFang LV ChangYou LI WeiHong YANG ZhanLan ZHAO Ying WANG DuJin LAI GuoQiao JIANG JianXiong XU YiZhuang WU JinGuang | 2009 | Science China Chemistry2009，52，11： | 4 |
| 3 | Caffeic acid phenethyl ester (CAPE) prevents transformation of human cells by arsenite (As) and suppresses growth of As-transformed cells 显示文摘 | Chengfeng Yang Jing Wu Ronghe Zhang | 2005 | Toxicology2005，213，12： | 1 |
| 4 | Prognostic Factors of Ampulla of Vater Carcinoma after Radical Surgery显示文摘OBJECTIVE Ampullary carcinoma is a rare disease with betterprognosis than other periampullary neoplasms. This studyinvestigated the association between clinicopathologic factors andprognosis after radical resection of ampulla of Vater carcinoma.METHODS Clinical data from 105 patients who underwentradical pancreaticoduodenectomy from January 1990 to December2005 were retrospectively analyzed by the Kaplan-Meier method,log-rank test, and the Cox proportional hazard model.RESULTS The in-hospital mortality rate was 8.6%, the lymphnode metastasis rate was 37.1%, and the five-year survival ratewas 42.8%. Pancreatic involvement (P = 0.027), tumor diameter (P= 0.008), T stage (P = 0.003), TNM stage (P < 0.001), and number ofmetastatic lymph nodes (P < 0.001) were associated with prognosiswhen the univariate analysis was used. Multivariate analysisshowed that the number of lymph node metastases (P < 0.001;OR: 1.923; CI: 1.367-2.705) and tumor diameter (P = 0.03; OR: 1.432;CI: 1.035-1.981) were the independent prognostic factors.CONCLUSION The number of metastatic lymph nodes andtumor diameter are important pathologic factors predictingprognosis of ampulla of Vater carcinoma after radical resection,and lymph node dissection during the radical surgery effectivelyimproves the survival rate. | Dongbing Zhao Yongkai Wu Yi Shan Chengfeng Wang Ping Zhao | 2009 | Chinese Journal of Clinical Oncology2009，6，2： | 1 |
| 5 | Method for detecting the pollution degree of naturally contaminated insulator based on hyperspectral characteristics显示文摘Insulator pollution degree detection is of great significance for preventing a flashover.Equivalent salt deposit density,leakage current,and surface pollution layer conductivity are commonly used to describe insulator pollution degree;however,all these parameters have limitations in field application and real‐time monitoring.Non‐contact detection methods,such as infrared thermal imaging and ultraviolet imaging,only image insulators in a specific band,which makes the extracted features limited.Hyperspectral technology is the new comprehensive image data technology based on imaging spectroscopy that has the advantages of multiband high resolution.Therefore,a method based on hyperspectral image and spectral characteristics is proposed to fully characterize natural pollution in-formation and accurately detect the pollution degree of insulators.The hyperspectral spectral line characteristics,image texture,and colour characteristic data of insulators were extracted and fused and then used to establish the pollution degree detection model on the basis of integrated learning classification algorithms.The results show that the model based on fusion data has an accuracy rate of 95.0%,which is more accurate than the model based on spectral line features only.Consequently,hyperspectral technology can realize the non‐contact detection of pollution degree and provide some guidance for cleaning the contamination of external insulation. | Chengfeng Yin Zhang Xiao Yujun Guo Chaoqun Shi Xueqin Zhang Guangning Wu | 2021 | High Voltage2021，6，6： | 1 |
| 6 | Structures and Receptor Binding of Hemagglutinins from Human-Infecting H7N9 Influenza Viruses显示文摘 | Yi Shi Wei Zhang Fei Wang Jianxun Qi Ying Wu Hao Song Feng Gao Yuhai Bi Yanfang Zhang Zheng Fan Chengfeng Qin Honglei Sun Jinhua Liu Joel Haywood Wenjun Liu Weimin Gong Dayan Wang Yuelong Shu Yu Wang Jinghua Yan George F. Gao | 2013 | Science2013，，6155： | 1 |
| 7 | High-performance carbon nanotube/polyaniline artificial yarn muscles working in biocompatible environments显示文摘Implantable artificial muscles are of great importance for muscle function restoration and physical augmentation but are still challenging.Herein,we report an artificial muscle by soaking-polymerization of polyaniline(PANI)inside a carbon nanotube(CNT)yarn.Working in aqueous biocompatible solutions,the yarn muscle generates a large contractile stroke of 17%and high isometric stress of 8 MPa at voltages lower than 2 V.The excellent performance can be ascribed to the large actuation volume that is enabled by the fast electrochemical redox of PANI confined in a coiled yarn structure.The actuation performance outperforms that of previously reported aqueous artificial yarn muscles.Moreover,the yarn muscle can work well and maintain excellent actuating performance in other biocompatible solutions such as normal saline and Na2SO4 aqueous solution,which makes the CNT/PANI yarn muscles suitable for implantable bionic applications.Finally,a biomimetic arm was fabricated to demonstrate the applications of the CNT/PANI yarn artificial muscle in implantable muscle,underwater robots,and soft exoskeletons. | Jian Qiao Yulong Wu Chengfeng Zhu Lizhong Dong Kunjie Wu Yulian Wang Wei Yang Min Li Jiangtao Di Qingwen Li | 2023 | Nano Research2023，16，3： | 0 |
| 8 | Evaluation of the Shallow Gas Hydrate Production Based on the Radial Drilling-Heat Injection-Back Fill Method显示文摘It has been evidenced that shallow gas hydrate resources are abundant in deep oceans worldwide.Their geological back-ground,occurrence,and other characteristics differ significantly from deep-seated hydrates.Because of the high risk of well construction and low production efficiency,they are difficult to be recovered by using conventional oil production methods.As a result,this paper proposes an alternative design based on a combination of radial drilling,heat injection,and backfilling methods.Multi-branch holes are used to penetrate shallow gas hydrate reservoirs to expand the depressurization area,and heat injection is utilized as a supplement to improve gas production.Geotechnical information collected from an investigation site close to the offshore production well in the South China Sea is used to assess the essential components of this plan,including well construction stability and gas production behavior.It demonstrates that the hydraulic fracturing of the 60mbsf overburden layer can be prevented by regulating the drilling fluid densities.However,the traditional well structure is unstable,and the suction anchor is advised for better mechanical performance.The gas produc-tion rate can be significantly increased by combining hot water injection and depressurization methods.Additionally,the suitable produc-tion equipment already in use is discussed. | CHEN Qiang WAN Yizhao WU Nengyou SUN Jianye WANG Jian LIU Changling LI Yanlong LI Chengfeng HU Gaowei | 2024 | Journal of Ocean University of China2024，23，1： | 0 |
| 9 | Microstructure Characterization of Bubbles in Gassy Soil Based on the Fractal Theory显示文摘The microscopic characterization of isolated bubbles in gassy soil plays an important role in the macroscopic physical properties of sediments and is a key factor in the study of geological hazards in gas-bearing strata.Based on the box-counting method and the pore fractal features in porous media,a fractal model of bubble microstructure parameters in gassy soil under different gas con-tents and vertical load conditions is established by using an industrial X-ray CT scanning system.The results show that the fractal di-mension of bubbles in the sample is correlated with the volume fraction of bubbles,and it is also restricted by the vertical load.The three-dimensional fractal dimension of the sample is about 1 larger than the average two-dimensional fractal dimension of all the slices from the same sample.The uniform porous media fractal model is used to test the equivalent diameter,and the results show that the variation of the measured pore diameter ratio is jointly restricted by the volume fraction and the vertical load.In addition,the measured self-similarity interval of the bubble area distribution is tested by the porous media fractal capillary bundle model,and the fitting curve of measured pore area ratio in a small loading range is obtained in this paper. | WU Chen LIN Guoqing LIU Lele LIU Tao LI Chengfeng GUO Zhenqi | 2024 | Journal of Ocean University of China2024，23，1： | 0 |
| 10 | A cluster positioning architecture and relative positioning algorithm based on pigeon fock bionics显示文摘Unmanned clusters can realize collaborative work,fexible confguration,and efcient operation,which has become an important development trend of unmanned platforms.Cluster positioning is important for ensuring the normal operation of unmanned clusters.The existing solutions have some problems such as requiring external system assistance,high system complexity,poor architecture scalability,and accumulation of positioning errors over time.Without the aid of the information outside the cluster,we plan to construct the relative position relationship with north alignment to adopt formation control and achieve robust cluster relative positioning.Based on the idea of bionics,this paper proposes a cluster robust hierarchical positioning architecture by analyzing the autonomous behavior of pigeon focks.We divide the clusters into follower clusters,core clusters,and leader nodes,which can realize fexible networking and cluster expansion.Aiming at the core cluster that is the most critical to relative positioning in the architecture,we propose a cluster relative positioning algorithm based on spatiotemporal correlation information.With the design idea of low cost and large-scale application,the algorithm uses intra-cluster ranging and the inertial navigation motion vector to construct the positioning equation and solves it through the Multidimensional Scaling(MDS)and Multiple Objective Particle Swarm Optimization(MOPSO)algorithms.The cluster formation is abstracted as a mixed direction-distance graph and the graph rigidity theory is used to analyze localizability conditions of the algorithm.We designed the cluster positioning simulation software and conducted localizability tests and positioning accuracy tests in diferent scenarios.Compared with the relative positioning algorithm based on Extended Kalman Filter(EKF),the algorithm proposed in this paper has more relaxed positioning conditions and can adapt to a variety of scenarios.It also has higher relative positioning accuracy,and the error does not accumulate over time. | Zhongliang Deng Hang Qi Chengfeng Wu Enwen Hu Runmin Wang | 2023 | Satellite Navigation2023，4，1： | 0 |
| 11 | Construction of Spirooxindole Skeleton Through Intramolecular Dieckmann Cyclization显示文摘A highly efficient and direct approach was developed to construct the structurally diverse spirooxindole skeleton,which is an important basic motif in natural products.Both the 3,30-pyrrolidonyl spirooxindoles and spiroindolin-2-one dlactones were 2smoothly obtained by the intramolecular Dieckmann cyclization of oxindoles in excellent yield under mild conditions. | Ting Wu Zhiqiang Pan Chengfeng Xia | 2017 | Natural Products and Bioprospecting2017，7，3： | 0 |
| 12 | Development of a rapid neutralizing antibody test for SARS-CoV-2 and its application for neutralizing antibody screening and vaccinated serum testing显示文摘Background:Since the outbreak of coronavirus disease(COVID-19),the high infection rate and mutation fre-quency of severe acute respiratory syndrome coronavirus 2(SARS-CoV-2),the causative agent,have contributed to the ongoing global pandemic.Vaccination has become the most effective means of controlling COVID-19.Tra-ditional neutralizing tests of sera are complex and labor-intensive,therefore,a rapid test for detecting neutralizing antibodies and antibody status post-immunization is needed.Methods:Based on the fact that antibodies exhibit neutralizing activity by blocking the binding of the S protein receptor-binding domain(S-RBD)to ACE2,we developed a rapid neutralizing antibody test,ACE2-Block-ELISA.To evaluate the sensitivity and specificity,we used 54 positive and 84 negative serum samples.We also tested the neutralizing activities of monoclonal antibodies(mAbs)and 214 sera samples from healthy individuals im-munized with the inactivated SARS-CoV-2 vaccine.Results:The sensitivity and specificity of the ACE2-Block ELISA were 96.3%and 100%,respectively.For neu-tralizing mAb screening,ch-2C5 was selected for its ability to block the ACE2-S-RBD interaction.A plaque assay confirmed that ch-2C5 neutralized SARS-CoV-2,with NT 50 values of 4.19,10.63,and 1.074μg/mL against the SARS-CoV-2 original strain,and the Beta and Delta variants,respectively.For the immunized sera samples,the neutralizing positive rate dropped from 82.14%to 32.16%within 4 months post-vaccination.Conclusions:This study developed and validated an ACE2-Block-ELISA to test the neutralizing activities of an-tibodies.As a rapid,inexpensive and easy-to-perform method,this ACE2-Block-ELISA has potential applications in rapid neutralizing mAb screening and SARS-CoV-2 vaccine evaluation. | Yuchang Li Mingyue Wang Hongzhen Wu Hui Zhao Lei Dong Yunfei Li Xiaofeng Li Ying Tang Sen Zhang Jing Li Chengfeng Qin Tao Jiang Yongqiang Deng Xiaoping Kang | 2022 | Infectious Medicine2022，1，2： | 0 |
| 13 | Identification of TP53 mutation-associated prognostic genes and investigation of the immune cell infiltration in patients with hepatocellular carcinoma显示文摘Primary liver cancer,which is mainly composed of hepatocellular carcinoma(HcC),is the sixth most common type of cancer worldwide and the thirdmost common cause of cancer mortality.1 The total number of mutations present in tumor specimens is called tumor mutation burden(TMB)and it is an emerging biomarker of immunotherapy response.2 TMB can predict clinical responses to immunotherapy such as ICl(immune checkpoint inhibitor)treatments and higher TMB is related to better survival.3 TP53,a gene encoding a tumor suppressor protein that triggers apoptosis and cell cycle arrest,is one of the most prevalent mutations in 25%-30%of HCC patients.4 Research shows that TP53 mutations in HCC patients are associated with advanced tumor grade and poor prognosis.5 To identify the TP53 mutation-related genes which can predict HCC patients'prognosis and explore the immune cell infiltration,we constructed a risk model based on six TP53 mutation-related genes which can accurately predict patients'prognosis.Besides,six immune cells with a similar expression pattern were identified in The Cancer Genome Atlas(TCGA)and International Cancer Genome Consortium(ICGC)databases. | Qijun Yang Lianke Gao Yuhan Xu Gaoquan Cao Yingcheng He Wenyige Zhang Xue Zhang Chengfeng Wu Kaili Liao Xiaozhong Wang | 2024 | Genes & Diseases2024，11，2： | 0 |
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