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| 1 | Identification of tumor associated single-chain Fv by panning and screening antibody phage library using tumor cells显示文摘AIM:To study the feasibility of panning and screeningphage-displaying recombinant single-chain variablefragment (ScFv) of anti-tumor monoclonal antibodiesfor fixed whole cells as the carriers of mAb-bindingantigens.ME,ODS: The recombinant phage displaying librariesfor anti-colorectal tumor mAb MC3Ab, MC5Ab andanti-gastric tumor mAb MGD1 was constructed.Panning and screening were carried outby means ofmodified fixation of colorectal and gastric tumor cellsexpressed the mAb-binding antigens. Concordance ofbinding specificity to tumor cells between phageclones and parent antibodies was analyzed. Thephage of positive clones was identified withcompetitive ELISA, and infected by E.coli HB2151 toexpress soluble ScFv.RESULTS:The ratio of positive clones to MC3-ScF-MC5-ScFv and MGD1-ScFv were 60 %, 24 % and 30 %. MC3-ScFv had Mr 32 000 confirmed by Western blot. Thespecificity to antigen had no difference between 4positive recombinant phage antibodies and MC3Ab.CONCLUSION:The modified process of fixing wholetumor cells is efficient, convenient and feasible to panand screen the phage-displaying ScFv of anti-tumormonoclonal antibodies. | Nie, YZ He, FT Li, ZK Wu, KC Cao, YX Chen, BJ Fan, DM | 2002 | World Journal of Gastroenterology2002，8，4： | 12 |
| 2 | 纳米粒疗法:一种新兴的癌症治疗方法显示文摘纳米粒具有在肿瘤细胞靶向定位和促进细胞吸收的功能,可在增强疗效的同时减少副作用,纳米粒疗法作为一种新型的癌症治疗方法正逐渐应用于临床。本文主要介绍纳米粒的主要结构及其区别于其他类型抗癌药物的特性,讨论目前第1代纳米粒疗法的临床应用以及处于临床前研究的最新纳米粒治疗系统,并总结纳米粒治疗系统目前已取得的成果和今后发展的趋势及挑战。 | 梁尔光 王玉丽 高春生 Davis ME Chen Z Shin DM | 2009 | 国际药学研究杂志2009，36，1： | 7 |
| 3 | Clinical value of com- bined detection of serum tumor markers in lung cancer diag- nosis 显示文摘 | Chen F Li WM Wang DM | 2008 | Sichuan Da Xue Xue Bao Yi Xue Ban2008，39，5： | 1 |
| 4 | Many chemokines in- cluding CCL20/MIP-3alpha display antimicrobial activity显示文摘 | Yang D Chen Q Hoover DM | 2003 | J Leuk Biol2003，74，： | 1 |
| 5 | Evolutionary patterns of hep- atitis B virus quasispecies under different selective pressures: correlation with antiviral efficacy显示文摘 | Liu F Chen L Yu DM | 2011 | Gut2011，60，9： | 1 |
| 6 | Essential tremor:diagnosis and treatment显示文摘 | Chen JJ Swope DM | 2003 | Pharmacotherapy2003，23，9： | 1 |
| 7 | Elevated plasma level of visfatinker-Bcell colony-enhancing factor in patients with type 2 dia- betes mellitus显示文摘 | Chen MP Chumg MP Chang DM | 2006 | J Soc Gynecol Investing2006，13，5： | 1 |
| 8 | Areca nut chewing is associated with metabolic syndrome : role of tumor necrosis factor- alpha, leptin, and white blood cell count in betel nut chewing- related metabolic derangements 显示文摘 | Chung FM Chang DM Chen MP | 2006 | Diabetes Care2006，29，7： | 1 |
| 9 | Evolutionary and biophysical relationships among thepapillomavirus E2 proteins 显示文摘 | Blakaj DM Femandez-Fuentes N Chen Z | 2009 | Frontiers In Bioscienee2009，14，1： | 1 |
| 10 | Improved genome annotation for Zymomonasmobilis 显示文摘 | Yang S Pappas KM Hauser LJ Land ML Chen GL Hurst GB PanC Kouvelis VN Typas MA Pelletier DA Klinqeman DM Chanq YJ Samatova NF Brown SD | 2009 | Nat Biotechnol2009，27，10： | 1 |
| 11 | Lenalidomide plus dexamethasone for relapsed multiple myeloma in North America 显示文摘 | WEBER DM CHEN C NIESVIZKY R | 2007 | N Engl J Med2007，357，21： | 1 |
| 12 | Pullout strength of pedicle screws with cement augmentation in severe osteoparosis:a comparative study between cannulated screws with cement injection and solid screws with cement pre-filling 显示文摘 | Chen LH Tai CL Lee DM et M | 2011 | BMC Museuloskelet Disord2011，12，： | 1 |
| 13 | Elevated plasma level of visfatin/pre-B cell colony-enhancing factor in patients with type 2 diabetes mellitus显示文摘 | Chen MP Chung FM Chang DM | 2006 | J Clin Endeerlnol Metab2006，91，21： | 1 |
| 14 | The diagnostic utility ofMOC31,BerEP4,RCC marker and CD10 in the classification of renal cell carcinoma and renal oncocytoma:an immunohistochemical analysis of 328 cases显示文摘 | Pan CC Chen PC Ho DM | 2004 | Histopathology2004，45，5： | 1 |
| 15 | Screening for liver cancer:results of a randomised controlled trial in Qidong,China显示文摘 | Chen JG Parkin DM Chen QG | 2003 | J Med Screen2003，10，4： | 1 |
| 16 | Defection of circulating gastric carcinoma associated antigen MC7- Ag in human sera using an established single determinant immuno - polymerase chain reaction technique显示文摘 | Ren J Chen Z Fan DM | 2000 | Cancer2000，88，2： | 1 |
| 17 | Dependence of cardiac ^11C- meta-hydroxyephedrine retention on norepinephrine transporter density显示文摘 | Raffel DM Chen W Sherman PS | 2006 | J Nucl Med2006，47，9： | 1 |
| 18 | Simultaneous determi-nation of malachite green, gentian violet and their leuco-metabo- lites in shrimp and salmon by liquid chromatographyetandem mass spectrometry with accelerated solvent extraction and auto solid-phase clean-up 显示文摘 | TAO Y F CHEN DM CHAO X Q | 2011 | Food Control2011，22，： | 1 |
| 19 | Seasonally dependent impacts of grazing on soil nitrogen mineralization and linkages to ecosystem functioning in Inner Mongolia grassland显示文摘 | Shan YM Chen DM Guan XX Zheng SX Chen HJ Wang MJ Bai YF | | 0，，： | 1 |
| 20 | Cost-Effectiveness of re- combinant Tissue-Type plasminogen activator within 3 hours of acute is- chemic stroke current evidence 显示文摘 | Boudreau DM Guzauskas GF Chen E | 2014 | Stroke2014，45，10： | 1 |
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