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| 1 | CRISPy-web: An online resource to design sgRNAs for CRISPR applications显示文摘CRISPR/Cas9-based genome editing has been one of the major achievements of molecular biology,allowing the targeted engineering of a wide range of genomes.The system originally evolved in prokaryotes as an adaptive immune system against bacteriophage infections.It now sees widespread application in genome engineering workflows,especially using the Streptococcus pyogenes endonuclease Cas9.To utilize Cas9,so-called single guide RNAs(sgRNAs)need to be designed for each target gene.While there are many tools available to design sgRNAs for the popular model organisms,only few tools that allow designing sgRNAs for non-model organisms exist.Here,we present CRISPy-web(http://gffzz2b22c00a04674870hv5bobkbxon906nqp.ffgz.tsg.suse.edu.cn/),an easy to use web tool based on CRISPy to design sgRNAs for any userprovided microbial genome.CRISPy-web allows researchers to interactively select a region of their genome of interest to scan for possible sgRNAs.After checks for potential off-target matches,the resulting sgRNA sequences are displayed graphically and can be exported to text files.All steps and information are accessible from a web browser without the requirement to install and use command line scripts. | Kai Blin Lasse Ebdrup Pedersen TilmannWeber Sang Yup Lee | 2016 | Synthetic and Systems Biotechnology2016，1，2： | 3 |
| 2 | 年龄相关性线粒体功能障碍加速动脉粥样硬化形成显示文摘老化是动脉粥样硬化的最强危险因素之一。然而,老化是否独立于慢性高血脂症而增加动脉粥样硬化的风险尚不清楚。该研究旨在确定高血脂症诱导前的血管老化是否会加速动脉粥样硬化的形成。研究者分析了幼年和老年正常血脂野生型(wild type,WT)、无疾病小鼠的主动脉,发现老化导致白细胞介素(interleukin,IL)6水平升高和线粒体功能障碍,并与线粒体自噬增加和其相关蛋白Parkin增加相关。在主动脉组织培养中,研究者发现随着老化的出现,线粒体功能障碍与IL-6存在正反馈关系。研究者通过诱导低密度脂蛋白受体的肝脏特异性降解,再结合10周的西方食物喂养,在老年和幼年小鼠中诱发急性高血脂症,发现老年WT小鼠的动脉粥样硬化增强。高血脂症进一步降低了老年小鼠的线粒体功能,增加了主动脉中Parkin的水平,但幼鼠则没有。 | 刘青(译) 叶鹏(摘、审校) Tyrrell DJ Blin M Song J Wood S Zhang M Beard DA Goldstein D | 2020 | 中华高血压杂志2020，28，2： | 2 |
| 3 | Noise analysis of an air-core fiber optic gyroscope显示文摘 | Blin S Digonnet M J F Kino G S | 2007 | IEEE Photonics Technology Letters2007，19，19： | 1 |
| 4 | Teleost liver hexokinase and glucokinase-like enzymes partral cDNA cloning and phylogenefic studies in rainbow trout (Oncorhychus myki-ss)、 common carp (Cyprimus carpto) and giltread seabream (Sparus aurata)显示文摘 | Blin C Panserat S Medale F | 1999 | Fish Physiology and Biochemistry1999，21，： | 1 |
| 5 | Neurobiology and clinical pharmacology of obsessive-compulsive disorder 显示文摘 | Joelle Micallef Olivier Blin | 2001 | Clin Neuropharmacology2001，24，4： | 1 |
| 6 | Physostigmine results in an increased decrement in brain glucose consumption in Alzheimer's disease 显示文摘 | Blin J Ivanoiu A De Volder A | 1998 | Psychopharmacology1998，136，： | 1 |
| 7 | A short overview on purification and conditioning of syngas produced by biomass gasification:Catalytic strategies,process intensification and new concepts显示文摘 | Richardson Y Blin J Julbe A | 2012 | Progress in Energy and Combustion Science2012，38，6： | 1 |
| 8 | Use of ordered mesoporous titania with semi-crystalline framework as photocatalyst 显示文摘 | Blin J L Stebe M J Roques-Carmes T | 2012 | Colloids and Surfaces A:Physicochemical and Engineering Aspects2012，407，5： | 1 |
| 9 | A purified population of multipotent cardiovascular progenitors derived from primate pluripotent stem cells engrafts in postmyocardial infarcted nonhuman primates显示文摘 | Blin Guillaume Nury David Stefanovic Sonia Neri Tui Guillevic Oriane Brinon Benjamin Bellamy Valérie Rücker-Martin Catherine Barbry Pascal Bel Alain Bruneval Patrick Cowan Chad Pouly Julia Mitalipov Shoukhrat Gouadon Elodie Binder Patrice | 2010 | Journal of Clinical Investigation2010，，4： | 1 |
| 10 | Profiling of regulatery microR- NA transcriptomes in various biological processes: a review 显示文摘 | Shah AA Meese E Blin N | 2010 | J Appl Genet2010，51，： | 1 |
| 11 | MSU-type mesoporous silica with well-tailored Ix)re sizes synthesized via an assembly of deca(ethylene oxide) oleyl ether surfactant and tetramethoxysilane silica precursor显示文摘 | Herrier G Blin J L Su B L | 2001 | Langmuir2001，47，： | 1 |
| 12 | The corrosion inhibition mechanism of new rare earth cinnamate compounds: Electrochemical studies 显示文摘 | BLIN F KOUTSOUKOS P KLEPETSIANIS P | 2007 | Electrochimica Acta2007，52，21： | 1 |
| 13 | Modal decomposition technique for multimode fibers 显示文摘 | Nguyen D M Blin S Nguyen T N | 2012 | Applied Optics2012，51，4： | 1 |
| 14 | The corrosion inhibition mechanism of new rare earth cinnamate compounds : Electrochemical studies 显示文摘 | Blin F Koutsoukos P Klepetsianis P | 2007 | Electrochimica Acta2007，52，21： | 1 |
| 15 | Down-regulation of TFF expression in gastrointestinal cell lines by cytokines and nuclear factor 显示文摘 | Dossinger V Kayademir T Blin N | 2002 | Cell Physiol Biochem2002，12，4： | 1 |
| 16 | Noise Analysis of an Air-core Fiber Optic Gyroscope显示文摘 | Stephane Blin Michel J F Digonnet Gordon S Kino | 2007 | IEEE Photonics Technology Letters2007，19，9： | 1 |
| 17 | Querying graphs in protein-pro-tein interactions networks using feedback vertex set显示文摘 | BLIN G SIKORA F VIALETTE S | 2010 | IEEE Transon Computational Biology and Bioinformatics2010，7，4： | 1 |
| 18 | A comparative review of new antipsychotics显示文摘 | Blin O | 1999 | Can J Psychiatry1999，44，： | 1 |
| 19 | Widely linear MVDR beamformers for the reception of an unknown signal corrupted by noncircular interferences显示文摘 | CHEVALIER P BLIN A | | 0，，11： | 1 |
| 20 | Hepatic glucokinase is induced by dietary carbohydrates in rainbow trout, gilthead seabream, and common carp 显示文摘 | Panserat S M6dale F Blin C | 2000 | American Journal Physiology Regulatory Integrative and Comparative Physiology2000，278，5： | 1 |
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