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| 1 | Bcl-x_L and Myeloid cell leukaemia-1 contribute to apoptosis resistance of colorectal cancer cells显示文摘AIM: To explore the role of Bcl-xL and Myeloid cell leukaemia (Mcl)-1 for the apoptosis resistance of colorectal carcinoma (CRC) cells towards current treat-ment modalities. METHODS: Bcl-xL and Mcl-1 mRNA and protein ex-pression were analyzed in CRC cell lines as well as human CRC tissue by Western blot,quantitative PCRand immunohistochemistry. Bcl-xL and Mcl-1 protein expression was knocked down or increased in CRC cell lines by applying specific siRNAs or expression plas-mids,respectively. After modulation of protein expres-sion,CRC cells were treated with chemotherapeutic agents,an antagonistic epidermal growth factor recep-tor (EGFR1) antibody,an EGFR1 tyrosine kinase inhibi-tor,or with the death receptor ligand TRAIL. Apoptosis induction and cell viability were analyzed. RESULTS: Here we show that in human CRC tis-sue and various CRC cell lines both Bcl-xL and Mcl-1 are expressed. Bcl-xL expression was higher in CRC tissue than in surrounding non-malignant tissue,both on protein and mRNA level. Mcl-1 mRNA expression was significantly lower in ma-lignant tissues. However,protein expression was slightly higher. Viability rates of CRC cells were significantly decreased after knock down of Bcl-xL expression,and,to a lower extent,after knock down of Mcl-1 expression. Furthermore,cells with reduced Bcl-xL or Mcl-1 expression was more sensitive towards oxaliplatin-and irinotecan-induced apoptosis,and in the case of Bcl-xL also towards 5-FU-induced apoptosis. On the other hand,upregulation of Bcl-xL by transfec-tion of an expression plasmid decreased chemothera-peutic drug-induced apoptosis. EGF treatment clearly induced Bcl-xL and Mcl-1 expression in CRC cells. Apop-tosis induction upon EGFR1 blockage by cetuximab or PD168393 was increased by inhibiting Mcl-1 and Bcl-xL expression. More strikingly,CD95-and TRAIL-induced apoptosis was increased by Bcl-xL knock down. CONCLUSION: Our data suggest that Bcl-xL and,to a lower extent,Mcl-1,are important anti-apoptotic factors in CRC. Specific downregulation of Bcl-xL is a promising approach to sensitize CRC cells towards chemotherapy and targeted therapy. | Henning Schulze-Bergkamen Roland Ehrenberg Lothar Hickmann Binje Vick Toni Urbanik Christoph C Schimanski Martin R Berger Arno Schad Achim Weber Steffen Heeger Peter R Galle Markus Moehler | 2008 | World Journal of Gastroenterology2008，14，24： | 4 |
| 2 | Identification of T cells that respond to serovar - specific regions of the Chlamydia trachoma tis major outer membrane protein in persons with serovar E infection显示文摘 | Arno JN Xie C Jones RB | 1998 | J Infect Dis1998，178，6： | 1 |
| 3 | Structural characterization and tensile properties of a high niobium containing gamma TiAl sheet obtained by powder metallurgical processing显示文摘 | ARNO B HELMUT C | 2004 | Intermetallics2004，12，3： | 1 |
| 4 | High miR‐21 expression from FFPE tissues is associated with poor survival and response to adjuvant chemotherapy in colon cancer显示文摘 | Naohide Oue Katsuhiro Anami Aaron J. Schetter Markus Moehler Hirokazu Okayama Mohammed A. Khan Elise D. Bowman Annett Mueller Arno Schad Manabu Shimomura Takao Hinoi Kazuhiko Aoyagi Hiroki Sasaki Masazumi Okajima Hideki Ohdan Peter R. Galle Wataru Yasui C | 2014 | Int. J. Cancer2014，，： | 1 |
| 5 | Non-destructive detection of hollow heart in potatoes using hyperspectral imaging显示文摘 | Angel D N Arno F Pilar C | 2011 | Image Analysis and Processing2011，6855，1： | 1 |
| 6 | Caris Acute respiratory distress syndrome:is it underrecognized in the pediatric intensive care unit 显示文摘 | Martin C J Kneyber Arno GA | 2008 | Pediatric Brief Report2008，，34： | 1 |
| 7 | Opposite contributions of glycinin and β-conglycinin derived peptides to the aggregation behavior of soy protein isolate hydrolysates显示文摘 | Kuipers Bas J H Gerrit A Arno C | 2006 | Food Biophysics2006，，1： | 1 |
| 8 | Refactoring Middleware with AspectJ 显示文摘 | Zhang C Hans- Arno J | 2003 | IEEE transactions on parallel and distributed systems2003，14，11： | 1 |
| 9 | Structural characterization and tensile properties of a high niobium containing gamma TiAl sheet obtained by powder metallurgical processing显示文摘 | ARNO B HELMUT C | 2004 | Intermetallics2004，12，3： | 1 |
| 10 | Heat-shock protein 60-reactive CD4^+ CD28^null T cells in patients with acute coronary syndromes显示文摘 | Zal B Kaski J C Arno G | 2004 | Circulation2004，109，： | 1 |
| 11 | Amplifications of the epidermal growth factor receptor gene(EGFR) are common in phyllodes tumors of the breast and are associated with tumor progression 显示文摘 | Kersting C Arno Kuijpcr A Schmidt H | 2006 | Lab Invest2006，86，1： | 1 |
| 12 | Heat-shock protein 60-reactive CD4+CD28null T cells in patients with acute coronary syndromes显示文摘 | ZAL B KASKI J C ARNO G | 2004 | Circulation2004，109，： | 1 |
| 13 | Intensity-modulated whole pelvic radiotherapy in women with gynecologic malignancies 1 1 Its contents are solely the responsibility of the authors and do not necessarily reflect the official views of the Illinois Department of Public Health.显示文摘 | Arno J Mundt Anthony E Lujan Jacob Rotmensch Steven E Waggoner S.Diane Yamada Gini Fleming John C Roeske | 2002 | International Journal of Radiation Oncology, Biology, Physics2002，，5： | 1 |
| 14 | A hybrid approach to reducing computed tomography metal artifacts in intracavitary brachytherapy 显示文摘 | Dan Xia Roeske John C Yu Lifeng Pelizzari Charles A Mundt Arno J and Pan Xiaochuan | 2005 | Brachytherapy2005，4，1： | 1 |
| 15 | Function of the medial meniscus in force transmission and stability 显示文摘 | Walker PS Arno S Bell C | 2015 | J Biomech2015，48，8： | 1 |
| 16 | A newinternal quality control chart based on biologicalvariation显示文摘 | Abdurrahman C Mustafa S Arno F | 2008 | Accred Qual Assur2008，13，2： | 1 |
| 17 | Non destructive detection of hollow heart in potatoes using hyperspectral imaging显示文摘 | Angel D N Arno F Pilaf C | 2011 | Image A nalysis and Processing2011，6855，18： | 1 |
| 18 | Acid-induced cold gelation of globular proteins: effect of protein aggregate characteristics and disulfide bonding on theological properties 显示文摘 | Arno C A Mireille W Els H A H | 2004 | Journal of Agricultural and Food Chemistry2004，52，3： | 1 |
| 19 | Heat-shock protein 60-reactive CD4+CD28 null T cells in patients with acute coronary syndromes显示文摘 | Zal B Kaski J C Arno G | 2004 | Circulation2004，109，10： | 1 |
| 20 | Impact of intensity-modulated radiotherapy on acute hematologic toxicity in women with gynecologic malignancies显示文摘 | Clark J Brixey John C Roeske Anthony E Lujan S.Diane Yamada Jacob Rotmensch Arno J Mundt | 2002 | International Journal of Radiation Oncology, Biology, Physics2002，，5： | 1 |
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