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| 1 | Quasispecies structure,cornerstone of hepatitis B virus infection: Mass sequencing approach显示文摘Hepatitis B virus(HBV)is a DNA virus with complex replication,and high replication and mutation rates,leading to a heterogeneous viral population.The population is comprised of genomes that are closely related,but not identical;hence,HBV is considered a viral quasispecies.Quasispecies variability may be somewhat limited by the high degree of overlapping between the HBV coding regions,which is especially important in the P and S gene overlapping regions,but is less significant in the X and preCore/Core genes.Despite this restriction,several clinically and pathologically relevant variants have been characterized along the viral genome.Next-generation sequencing(NGS)approaches enable high-throughput analysis of thousands of clonally amplified regions and are powerful tools for characterizing genetic diversity in viral strains.In the present review,we update the information regarding HBV variability and present a summary of the various NGS approaches available for research in this virus.In addition,we provide an analysis of the clinical implications of HBV variants and their study by NGS. | Francisco Rodriguez-Frias Maria Buti David Tabernero Maria Homs | 2013 | World Journal of Gastroenterology2013，19，41： | 11 |
| 2 | Characteristics of vertical ozone distribution in the lower troposphere in the Yangtze River Delta at Lin'an in the spring of 2001显示文摘We analyzed vertical distributions of ozone (O3) in the lower troposphere (< 5 km above ground) at Lin’an (119.75°E, 30.30°N), Zhejiang Province using electrochemical concen- tration cell (ECC) ozonesonde data obtained from February 21 to April 13, 2001. The results showed that the vertical O3 distributions are controlled by metrological conditions and the char- acteristics of O3 profiles are related to those of wet bulb potential temperature and wind field. O3 below 2 km showed that the strongest variability and enhanced O3 mixing ratios were associated with easterly winds that blow pollutants from the upwind source region of the Yangtze River Delta (YRD) region. Vertical O3 profiles below 5 km can be grouped into 5 categories: (1) peak mixing ratio type, (2) well-mixed type, (3) layered-structure type, (4) episodic pollution type and (5) alti- tudinal increasing type. Vertical distributions of O3 affected by regional transport of polluted air masses were investigated. Transport of polluted air from high latitudes of northern China, ac- companying subsiding motion of air and stagnant atmospheric conditions are important factors that lead to high mixing ratios of O3 at Lin’an. The stagnant atmospheric conditions associated with a continental high pressure system and pollution plume transported from the YRD and cen- tral-eastern China also lead to regional accumulation of O3 and high O3 mixing ratio at Lin’an. Long-range transport of O3 and pollutants from the Pearl River Delta in South China and in-situ O3 formation also resulted in elevated O3 mixing ratios at around 1 km altitudes and layered O3 distribution in the lower troposphere. | ZHENG Xiangdong1, CHAN Chuenyu2, CUI Hong2, QIN Yu3, CHAN Loyan2, ZHENG Yongguang3 & LEE Yusiang2 1. Chinese Academy of Meteorological Sciences, Beijing 100081, China 2. Department of Civil and Structural Engineering, Hong Kong Polytechnic University, Hung Hom, Hong Kong, China 3. Department of Atmospheric Sciences, Peking University, Beijing 100871, China | 2005 | Science China Earth Sciences2005，48，9： | 6 |
| 3 | Modeling uncertainty in geographic information and analysis显示文摘Uncertainty modeling and data quality for spatial data and spatial analyses are im-portant topics in geographic information science together with space and time in geography,as well as spatial analysis. In the past two decades,a lot of efforts have been made to research the uncertainty modeling for spatial data and analyses. This paper presents our work in the research. In particular,four progresses in the re-search are given out: (a) from determinedness-to uncertainty-based representation of geographic objects in GIS; (b) from uncertainty modeling for static data to dy-namic spatial analyses; (c) from modeling uncertainty for spatial data to models; and (d) from error descriptions to quality control for spatial data. | SHI WenZhong Advanced Research Centre for Spatial Information Technology,Department of Land Surveying and Geo-Informatics,the Hong Kong Polytechnic University,Hung Hom,Kowloon,Hong Kong,China | 2008 | Science China(Technological Sciences)2008，51，S1： | 6 |
| 4 | Quasispecies dynamics in main core epitopes of hepatitis B virus by ultra-deep-pyrosequencing显示文摘AIM:To investigate the variability of the main immunodominant motifs of hepatitis B virus(HBV) core gene by ultra-deep-pyrosequencing(UDPS).METHODS:Four samples(2 genotype A and 2 genotype D) from 4 treatment-na ve patients were assessed for baseline variability.Two additional samples from one patient(patient 4,genotype D) were selected for analysis:one sample corresponded to a 36-mo treatment-free period from baseline and the other to the time of viral breakthrough after 18 mo of lamivudine treatment.The HBV region analyzed covered amino acids 40 to 95 of the core gene,and included the two main epitopic regions,Th50-69 and B74-84.UDPS was carried out in the Genome Sequencer FLX system(454 Life Sciences,Roche).After computer filtering of UDPS data based on a Poisson statistical model,122 813 sequences were analyzed.The most conserved position detected by UDPS was analyzed by site-directed mutagenesis and evaluated in cell culture.RESULTS:Positions with highest variability rates were mainly located in the main core epitopes,confirming their role as immune-stimulating regions.In addition,the distribution of variability showed a relationship with HBV genotype.Patient 1(genotype A) presented the lowest variability rates and patient 2(genotype A) had 3 codons with variability higher than 1%.Patient 3 and 4(both genotype D) presented 5 and 8 codons with variability higher than 1%,respectively.The median baseline frequencies showed that genotype A samples had higher variability in epitopic positions than in the other positions analyzed,approaching significance(P = 0.07,sample 1 and P = 0.05,sample 2).In contrast,there were no significant differences in variability between the epitopic and other positions in genotype D cases.Interestingly,patient 1 presented a completely mutated motif from amino acid 64 to 67(E 64 LMT 67),which is commonly recognized by T helper cells.Additionally,the variability observed in all 4 patients was particularly associated with the E 64 LMT 67 motif.Codons 78 and 79 were highly conserved in all samples,in keeping with their involvement in the interaction between the HBV virion capsid and the surface antigens(HBsAg).Of note,codon 76 was even more conserved than codons 78 and 79,suggesting a possible role in HBsAg interactions or even in hepatitis B e antigen conformation.Sequential analysis of samples from patient 4(genotype D) illustrated the dynamism of the HBV quasispecies,with strong selection of one minor baseline variant coinciding with a decrease in core variability during the treatment-free and lamivudinetreated period.The drop in variability seemed to result from a 'steady state' situation of the HBV quasispecies after selection of the variant with greatest fitness.CONCLUSION:Host immune pressure seems to be the main cause of HBV core evolution.UDPS analysis is a useful technique for studying viral quasispecies. | Maria Homs Maria Buti David Tabernero Josep Quer Alex Sanchez Noelia Corral Rafael Esteban Francisco Rodriguez-Frias | 2012 | World Journal of Gastroenterology2012，18，42： | 5 |
| 5 | 糖尿病合并视网膜脱离术后高眼压的处理及危险因素探讨显示文摘目的:观察糖尿病合并孔源性视网膜脱离患者行玻璃体切割术后短期内眼压的波动情况并探讨可能的危险因素及有效的治疗方法。方法:连续收集100例糖尿病合并高度近视及孔源性视网膜脱离的患者并分为两组:试验组和对照组,试验组患者术后使用妥布霉素地塞米松滴眼液。分别监测术眼玻璃体切割术术前及术后的眼压情况,神经纤维层厚度(RNFL)和视盘参数,同时记录对侧眼术前的情况。实验数据采用Graphpad 7.0进行分析。结果:所有的糖尿病患者中有42眼在玻璃体切割术后1wk内出现眼压升高,其中试验组31眼,而对照组11眼。术后5d-1wk及1mo时两组间差异有显著统计学意义。随后,39眼经过治疗后眼压下降,3眼接受了青光眼滤过手术。与眼压升高前的视盘各参数相比,眼压升高后,平均及下方RNFL厚度变薄,盘沿面积变小,同时视杯容积和垂直杯盘比增加。眼压升高患者对侧眼的RNFL厚度相比于从未出现高压眼的患者明显变薄。结论:玻璃体切割术术后连续使用激素类药物是糖尿病合并视网膜脱离患者术后眼压升高的危险因素,并且可疑青光眼患者使用激素后眼压更易升高。 | 石蕊 谢安明 李迪 车选义 Rechard Hom | 2019 | 国际眼科杂志2019，19，7： | 5 |
| 6 | THREE-DIMENSIONAL NUMERICAL MODELINGOF COHESIVE SEDIMENT TRANSPORT BY TIDAL CURRENTIN PEARL RIVER ESTUARV显示文摘A three-dimensional numerical model of suspended sediment transport taking into account the effects of cohesiveness between sediments is presented. An efficient operator splitting scheme is employed to solve the equations of mass conservation, momentum and suspended sediment transport. The splitting method partitions a time step into three fractional time steps according to physical phenomena. In the first step, advection and Coriolis force are solved by Eulerian-Lagrangian method (ELM). Horizontal diffusion is approximated by implicit finite element method (FEM) for each horizontal layer in the second step. In the last step, vertical diffusion and pressure gradient are discretized by implicit finite difference method (FDM) for each nodal column. The continuity equation is solved by implicit FEM within an entire time step. The sigma coordinate system for vertical columns and nine-node isoparameteric finite elements for horizontal planes are employed in the model. Empirical functions specifically developed for cohesive sediments in China have been incorporated into the model. The model has been applied to investigate the suspended sediment concentration and seabed evolution in the Pearl River Estuary (PRE) in China. Computed results show that the distribution of sediment concentration and seabed evolution is in agreement with field measurements. In addition, the effect of the size of time step on result is discussed. The computed result shows that larger time steps can be used in this model for saving computation time without losing much accuracy. | Yong CHEN1, Onyx W.H. WAI2, Y. S. Li3 and Qimiao LU4 (l 2,3 Department of Civil and Structural Engineering, The Hong Kong Polytechnic University. Hung Hom, Kowloon, Hong Kong 4,Department of Civil Engineering, Daltech, Dalhousie University, Halifax, Can | 1999 | International Journal of Sediment Research1999，14，2： | 4 |
| 7 | Relationship specialization amongst sources and receivers of social support and its correlations with loneliness and subjective well-being: A cross sectional study of Nepalese older adults显示文摘 | Hom Nath Chalise Tami Saito Miyako Takahashi Ichiro Kai | 2006 | Archives of Gerontology and Geriatrics2006，，3： | 2 |
| 8 | Subtype-Specific MEK-PI3 Kinase Feedback as a Therapeutic Target in Pancreatic Adenocarcinoma显示文摘 | Olga K. Mirzoeva Eric A. Collisson Peter M. Schaefer Byron Hann Yun K. Hom Andrew H. Ko W. Michael Korn | 2013 | Molecular Cancer Therapeutics2013，，10： | 2 |
| 9 | Regulation of mitochondrial fission by intracellular Ca 2+ in rat ventricular myocytes显示文摘 | Jennifer Hom Tianzheng Yu Yisang Yoon George Porter Shey-Shing Sheu | 2010 | BBA - Bioenergetics2010，，6： | 2 |
| 10 | Predictors of mortality in adult patients with congestive heart failure receiving nesifitide-retrospective analysis showing a potential adverse interaction between nesifitide and acute renal dysfunction 显示文摘 | Iglesias J DePalma L Hom D | 2008 | Nephrel Dial Transplant2008，23，： | 1 |
| 11 | A comparison of three models of ventilation with the use of an adult circle system in an infant lung model 显示文摘 | TOBIN M J STEVENSON G W HOM B J | 1998 | Anesth Analg1998，87，3： | 1 |
| 12 | Inflammatory pain alters blood-brain barrier permeability and tight junctional protein expression显示文摘 | Huber JD Witt KA Hom S | 2001 | Am J Physiol Heart Circ Physiol2001，280，： | 1 |
| 13 | A meta - analysis ofantecedents and correlates of employee turnover: Update, moderatortests,and research implications for the millennium 显示文摘 | GRIFFETH R W HOM P W GAERTNER S | 2000 | Journal ofManagement2000，26，3： | 1 |
| 14 | Use of biofuels to producehydrogen (reformation processes)显示文摘 | de la Piscina P R Homs N | 2008 | Chemical SocietyReviews2008，37，11： | 1 |
| 15 | Review of recent genome-wide association scans in lupus显示文摘 | Graham RR Hom G Ortmann W | | 0，，6： | 1 |
| 16 | Nociceptive inhibition prevents inflammatory pain induced changes in the blood-brain barrier显示文摘 | Campos CR Ocheltree SM Hom S | | 0，，： | 1 |
| 17 | The estimation of ammonia using the indophenol reaction显示文摘 | Hom D.B C.R.Squire | | 0，，： | 1 |
| 18 | Prospective evaluation of vascular complications after liver transplantation : comparison of conventional and microbubble contrast-enhanced US 显示文摘 | Hom BK Shrestha R Palmer SL | 2006 | Radiology2006，241，1： | 1 |
| 19 | Experience with an enteral diet with fiber and a high fat content in ICU patients with glucose intolerance显示文摘 | Celaya S Sanz A Homs C | 1992 | Nutr Hosp1992，7，4： | 1 |
| 20 | Liver and skeletal muscle mitochondrial function following burn injury 显示文摘 | Aprille JR Hom JA Rulfs J | 1977 | J Trauma1977，17，4： | 1 |
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