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| 1 | Genetic improvement of Trichoderma ability to induce systemic resistance显示文摘The beneficial applications of Trichoderma spp. in agriculture include not only the control of plant pathogens, but also the improvement of plant growth, micronutrient availability, and plant tolerance to abiotic stress. In addition, it has been suggested that these fungi are able to increase plant disease resistance by activating induced systemic resistance (ISR) . The mode of action of these beneficial fungi in the Trichoderma -plant-pathogen interaction are many, complex and not completely understood. Numerous lytic enzymes have been characterized, the encoding genes (ech42 gluc78, nag1 from T. atroviride strain P1) cloned, and their role in biocontrol demonstrated. The corresponding biocontrol-related inducible promoters have been used in a reporter system based on the Aspergillus niger glucose oxidase gene (goxA) to monitor biocontrol activity. Glucose oxidase catalyzes the oxygen-dependent oxidation of D-glucose to D-glucono-1,5-lactone and hydrogen peroxide; this latter compound is known to have an antifungal effect and activate the plant defence cascade, thus increasing resistance to pathogen attack. T. atroviride P1 transformants with various promoters gox were tested as seed coating treatments on bean seeds planted in soil infested with a soilborne fungal pathogen. Successively, the emergent leaves were inoculated with a foliar pathogen to determine the effect of the GOX transformants on biocontrol and resistance to pathogen attack. Inoculations with the P1-GOX transformants not only reduced disease symptoms caused by a soil pathogen, but also the lesions of various foliar pathogens applied far from the Trichoderma colonization, thus activating ISR. A similar approach is being use to genetically improve T. harzianum T22, a rhizosphere competent and commercially marketed strain not transformed yet, by using four different gox gene constructs under the control of constitutive and inducible promoters. Plasmids have been introduced in Trichoderma by protoplasts co-transformation. hygromicin resistant progeny selected, and mitotically stable transformants analysed to confirm the presence of the novel enzyme activity. Progenies are being tested for biocontrol ISR inducing activity. | Ciliento R Woo S L Di Benedetto P Ruocco M Scala F Soriente I Ferraioli S Brunner K Zeilinger S Mach R L Lorito M | 2004 | 浙江大学学报(农业与生命科学版)2004，30，4： | 2 |
| 2 | A multifrequency magnetic induction tomography system using planar gradiometers: data collection and calibration显示文摘 | ROSELL-FERRER J MERWA R BRUNNER P | 2006 | Physiol Meas2006，27，： | 1 |
| 3 | Attenuation of myocardial ischemia/reperfusion injury in mice with myocyte-specific overexpression of endothelial nitric oxide synthase显示文摘 | Maier R Andrew P | 2003 | Cardiovasc Res2003，57，1： | 1 |
| 4 | Open reduction and fixation of medial Moore type Ⅱ fractures of the tibial plateau by a direct dorsal approach显示文摘 | Brunner A Honigmann P Horisberger M | 2009 | Arch Orthop Trauma Surg2009，129，9： | 1 |
| 5 | Pulmonary embolism caused by acrylic cement:A rare complication of percutaneous vertebroplasty显示文摘 | Padovani B Kasmel O brunner P | 1999 | Am J Nenroradid1999，20，3： | 1 |
| 6 | Emotional stress in pregnancy predicts human infant reactivity 显示文摘 | Mohler E Parzer P Brunner R | 2006 | Early Human Development2006，82，11： | 1 |
| 7 | Procedural complications following diagnostic coronary angiography are related to the operator's experience and the catheter size 显示文摘 | Ammann P Brunner - La Rocca HP Angehrn W | 2003 | Catheter Cardiovasc Interv2003，59，1： | 1 |
| 8 | Does the 'P300' speller depend on eye gaze 显示文摘 | BRUNNER P JOSHI S BRISKIN S | 2010 | Journal of Neural Engineering2010，7，5： | 1 |
| 9 | Pulmonary erllbolism caused by acrylic cement:a rare corn plication percutaneous venebmplasty 显示文摘 | PADOVANI B KASMEI O BRUNNER P | 1999 | Am J Nenroradid1999，20，3： | 1 |
| 10 | pulmonary embolism caused by acrylic cement:a rare complication of percutaneous vertebroplasty显示文摘 | Padovani B Kasriel O Brunner P | 1999 | Am J Neuroradiol1999，20，3： | 1 |
| 11 | Genome-wide analysis of Aux/IAA and ARF gene families in Populus trichocarpa显示文摘 | Kalluri U C Difazio S P Brunner A M | 2007 | BMC Plant Biology2007，7，： | 1 |
| 12 | Solution of the inverse problem of magnetic induction tomography (MIT)显示文摘 | Merwa R Hollaus K Brunner P | 2005 | Physiol Meas2005，26，： | 1 |
| 13 | Allelic series of four powdery mildew resistance genes at the Pm3 locus in hexaploid bread wheat显示文摘 | Srichumpa P Brunner S Keller B | 2005 | Plant Physiology2005，139，： | 1 |
| 14 | Percutaneous treatment in patients presenting with malignant cardiac tamponade显示文摘 | Marcy PY Bondiau PY Brunner P | 2005 | Eur Radiol2005，15，9： | 1 |
| 15 | Pulmonary embolism caused by acrylic cement a rare complication of percutaneous vertebroplasty显示文摘 | Kasriel O Brunner P | 1999 | Am J Neuroradiol1999，20，： | 1 |
| 16 | Blood levels of angiogenin and vascular endothelial growth factor are elevated in myelodysplastic syndrome and in actue myeloid leukemia 显示文摘 | Brunner B Gunsilius E Schumacher P | 2002 | Journal of Heamatotherapy & Stem Cell Research2002，11，： | 1 |
| 17 | 4-Nonylphenol in sewage sludge: accumulation of toxic metabolites from nonionie surfaetants显示文摘 | GIGER W BRUNNER P H SCHAFFNER C | 1984 | Science1984，225，4662： | 1 |
| 18 | Favourable dermal penetration of diclofenac after administration to the skin using a novel spray gel formulation显示文摘 | Brunner M Dehghanyar P Seigfried B | 2005 | Br J Clin Pharmacol2005，60，5： | 1 |
| 19 | Open reduction andfixation of medial Moore typeⅡfractures of the tibial plateau by a direct dorsal approach显示文摘 | Brunner A Honigmann P Horisberger M | 2009 | Arch Orthop Trauma Surg2009，129，9： | 1 |
| 20 | MAC reduction of isoflurane by sufentanil显示文摘 | Brunner MD Braithwaite P Jhaveri R | 1994 | Br J Anaesth1994，72，1： | 1 |
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