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| 1 | Genetic improvement of Trichoderma ability to induce systemic resistance显示文摘The beneficial applications of Trichoderma spp. in agriculture include not only the control of plant pathogens, but also the improvement of plant growth, micronutrient availability, and plant tolerance to abiotic stress. In addition, it has been suggested that these fungi are able to increase plant disease resistance by activating induced systemic resistance (ISR) . The mode of action of these beneficial fungi in the Trichoderma -plant-pathogen interaction are many, complex and not completely understood. Numerous lytic enzymes have been characterized, the encoding genes (ech42 gluc78, nag1 from T. atroviride strain P1) cloned, and their role in biocontrol demonstrated. The corresponding biocontrol-related inducible promoters have been used in a reporter system based on the Aspergillus niger glucose oxidase gene (goxA) to monitor biocontrol activity. Glucose oxidase catalyzes the oxygen-dependent oxidation of D-glucose to D-glucono-1,5-lactone and hydrogen peroxide; this latter compound is known to have an antifungal effect and activate the plant defence cascade, thus increasing resistance to pathogen attack. T. atroviride P1 transformants with various promoters gox were tested as seed coating treatments on bean seeds planted in soil infested with a soilborne fungal pathogen. Successively, the emergent leaves were inoculated with a foliar pathogen to determine the effect of the GOX transformants on biocontrol and resistance to pathogen attack. Inoculations with the P1-GOX transformants not only reduced disease symptoms caused by a soil pathogen, but also the lesions of various foliar pathogens applied far from the Trichoderma colonization, thus activating ISR. A similar approach is being use to genetically improve T. harzianum T22, a rhizosphere competent and commercially marketed strain not transformed yet, by using four different gox gene constructs under the control of constitutive and inducible promoters. Plasmids have been introduced in Trichoderma by protoplasts co-transformation. hygromicin resistant progeny selected, and mitotically stable transformants analysed to confirm the presence of the novel enzyme activity. Progenies are being tested for biocontrol ISR inducing activity. | Ciliento R Woo S L Di Benedetto P Ruocco M Scala F Soriente I Ferraioli S Brunner K Zeilinger S Mach R L Lorito M | 2004 | 浙江大学学报(农业与生命科学版)2004，30，4： | 2 |
| 2 | A multifrequency magnetic induction tomography system using planar gradiometers: data collection and calibration显示文摘 | ROSELL-FERRER J MERWA R BRUNNER P | 2006 | Physiol Meas2006，27，： | 1 |
| 3 | Attenuation of myocardial ischemia/reperfusion injury in mice with myocyte-specific overexpression of endothelial nitric oxide synthase显示文摘 | Maier R Andrew P | 2003 | Cardiovasc Res2003，57，1： | 1 |
| 4 | Testosterone inhibits expression of inducible nitric oxide synthase in murine macrophages显示文摘 | Brunner M Moeslinger T | 2000 | Life Sci2000，68，4： | 1 |
| 5 | Emotional stress in pregnancy predicts human infant reactivity 显示文摘 | Mohler E Parzer P Brunner R | 2006 | Early Human Development2006，82，11： | 1 |
| 6 | Colonic spread and serum pharmacokinetics of budesonide foam in patients with mildly to moderately active ulcerative colitis显示文摘 | Brunner M Vogelsang H Greinwald R | 2005 | Aliment Pharmacol Ther2005，22，5： | 1 |
| 7 | G-CSF treatment after myocardial infarction:impact on bone marrow-derived vs cardiac progenitor cells显示文摘 | Brunner S Huher BC Fischer R | 2008 | Exp Hematol2008，36，6： | 1 |
| 8 | Solution of the inverse problem of magnetic induction tomography (MIT)显示文摘 | Merwa R Hollaus K Brunner P | 2005 | Physiol Meas2005，26，： | 1 |
| 9 | Service Management in Multi-party Active Networks 显示文摘 | Brunner M Stadler R | 2000 | IEEE Communications Magazine2000，38，3： | 1 |
| 10 | Service management multiparty active networks显示文摘 | Brunner M TIK Stadler R | 2000 | IEEE Communications Magazine2000，38，3： | 1 |
| 11 | Improvement of the fungal biocontrol agent Trichoderma atroviride to enhance both antagonism and induction of plant systemic disease resistance 显示文摘 | Brunner K Zeilinger S Ciliento R | 2005 | Applied and Environmental Microbiology2005，71，7： | 1 |
| 12 | Vasopressin in CSF and plasma in depressed suicide attempters: preliminary results 显示文摘 | Brunner J Keck ME Landgraf R | 2002 | Eur Neuropsychopharmacol2002，12，5： | 1 |
| 13 | Toward an integrated assessment of the impacts of extreme wind events on Barrow,Alaska显示文摘 | Lynch A H Curry J A Brunner R D | 2004 | Bulletin of American Meteorology Society2004，85，： | 1 |
| 14 | Clinical benefit of reconstruction of dis-located or subluxated hip joints in patients with spastic cerebralpalsy显示文摘 | Brunner R Baumann JU | 1994 | J Pediatr Orthop1994，14，： | 1 |
| 15 | A novel approach for the formation of Mg(OH)2/MgO nanowhiskers on magnesium: Rapid anodization in chloride containing solutions 显示文摘 | Hahn R Brunner J G Kunze J | 2008 | Electrochemistry Communications2008，10，2： | 1 |
| 16 | 显示文摘 | Hagenmaier H Brunner H Hagg R | 1987 | Environment Science and Technology1987，21，： | 1 |
| 17 | Service Management in Multi-Party Active Networks 显示文摘 | Brunner M Stadler R | 2000 | IEEE Communications Magazine2000，38，3： | 1 |
| 18 | Diagnosis of viral myocarditis by cardiac magnetic resonance and viral genome detection in peripheral blood显示文摘 | Jeserich M Brunner E Kandolf R | 2013 | The international journal of cardiovascular imaging2013，29，1： | 1 |
| 19 | Determination of lactose in milk productsby high performance liquid chromatography显示文摘 | EUBER J R BRUNNER J R | 1979 | Journal of Dairy Science1979，62，5： | 1 |
| 20 | Validation of urea as anendogenous reference compound for the in vivo calibration of Micro-dialysis probes显示文摘 | Brunner M Joukhadar C Schmid R | 2000 | Life Sci2000，67，： | 1 |
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