|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Genetic improvement of Trichoderma ability to induce systemic resistance显示文摘The beneficial applications of Trichoderma spp. in agriculture include not only the control of plant pathogens, but also the improvement of plant growth, micronutrient availability, and plant tolerance to abiotic stress. In addition, it has been suggested that these fungi are able to increase plant disease resistance by activating induced systemic resistance (ISR) . The mode of action of these beneficial fungi in the Trichoderma -plant-pathogen interaction are many, complex and not completely understood. Numerous lytic enzymes have been characterized, the encoding genes (ech42 gluc78, nag1 from T. atroviride strain P1) cloned, and their role in biocontrol demonstrated. The corresponding biocontrol-related inducible promoters have been used in a reporter system based on the Aspergillus niger glucose oxidase gene (goxA) to monitor biocontrol activity. Glucose oxidase catalyzes the oxygen-dependent oxidation of D-glucose to D-glucono-1,5-lactone and hydrogen peroxide; this latter compound is known to have an antifungal effect and activate the plant defence cascade, thus increasing resistance to pathogen attack. T. atroviride P1 transformants with various promoters gox were tested as seed coating treatments on bean seeds planted in soil infested with a soilborne fungal pathogen. Successively, the emergent leaves were inoculated with a foliar pathogen to determine the effect of the GOX transformants on biocontrol and resistance to pathogen attack. Inoculations with the P1-GOX transformants not only reduced disease symptoms caused by a soil pathogen, but also the lesions of various foliar pathogens applied far from the Trichoderma colonization, thus activating ISR. A similar approach is being use to genetically improve T. harzianum T22, a rhizosphere competent and commercially marketed strain not transformed yet, by using four different gox gene constructs under the control of constitutive and inducible promoters. Plasmids have been introduced in Trichoderma by protoplasts co-transformation. hygromicin resistant progeny selected, and mitotically stable transformants analysed to confirm the presence of the novel enzyme activity. Progenies are being tested for biocontrol ISR inducing activity. | Ciliento R Woo S L Di Benedetto P Ruocco M Scala F Soriente I Ferraioli S Brunner K Zeilinger S Mach R L Lorito M | 2004 | 浙江大学学报(农业与生命科学版)2004，30，4： | 2 |
| 2 | Role of four major cellulases in triggering of cellulase gene expression by cellulose in Trichoderma reesei 显示文摘 | Seiboth B Hakola S Mach R L | 1997 | J Bacteriol1997，179，17： | 1 |
| 3 | Role of four major cellulases in triggering of cellulase gene expression by cellulose in Trichoderma reesei显示文摘 | Seiboth B Hakola S Mach R L | 1997 | Journal of Bacteriology1997，179，17： | 1 |
| 4 | Role of four major cellulases in triggering of ceUulase gene expression by cellulose in Trichoderma reesei 显示文摘 | SE1BOTH B HAKOLA S MACH R L | 1997 | J Baeteriol1997，179，17： | 1 |
| 5 | Optimisation of porcine oocyte activation following nuclear transfer显示文摘 | TAO T MACH~TY Z ABEYDEERA L R | 2000 | Zygote2000，8，1： | 1 |
| 6 | Generation of Arabidopsis thalianna plants with complex N-glycans lacking β1,2-linked xylose and coreα1,3-linked fucose显示文摘 | Strasser R Altmann F Mach L | 2004 | FEBS Lett2004，561，13： | 1 |
| 7 | Phylogenetic relationship of Fusarium langsethiae to Fusarium poaeand Fusarium sporotrichioidesas inferred by IGS,ITS,β-tubulin sequences and UP-PCR hybridization analysis显示文摘 | Mattila T Mach R L Alekhina I A | 2004 | International Journal of Food Microbiology2004，95，3： | 1 |
| 8 | Ca2+-calmodulin antagonists interfere with xylanase formation andsecretion in Trichoderma reesei显示文摘 | MACH R L ZEILINGER S KRISTUFEK D | 1998 | Biochimica et BiophysicaActa1998，1403，： | 1 |
| 9 | Regulation of gene expressionin industrial fungi:Trichoderma显示文摘 | MACH R L ZEILINGER S | 2003 | Applied MicrobiologicalBiotechnology2003，60，： | 1 |
| 10 | Role of fourmajor cellulases in triggering of cellulose gene expression bycellulose in Trichoderma reesei显示文摘 | SEIBOTH B HAKOLA S MACH R L | 1997 | Journal of Bacteriology1997，179，17： | 1 |
| 11 | Phylogenetic relation- ship of Fusarium langsethiae to Fusarium poae and Fusarium spo- rotrichioides as inferred by IGS, ITS, h-tubulin sequences and UP- PCR hybridization analysis显示文摘 | Yli-Mattila T Mach R L Alekhina I A | 2004 | International Journal of Food Micro- biology2004，95，： | 1 |
| 12 | Trichoderma:from genes to biocontrol显示文摘 | KUBICEK C P MACH R L PETERBAUER C K | 2001 | Journal of plant pathology2001，83，2： | 1 |
| 13 | Regulation of gene expression in industrial fungi:Trichoderma显示文摘 | Mach R L Zeilinger S | | 0，，05： | 1 |
| 14 | Determination of Escherichia coli Contamination with Chromocult Coliform Agar Showed a High Level of Discrimination Efficiency for Differing Fecal Pollution Levels in Tropical Waters of Kampala, Uganda显示文摘 | Byamukama D Kansiime F Mach R L | | Appl En0，，： | 1 |
| 15 | Xyr1 receives the lactose induction signal and regulates lactose metabolism in Hypocrea jecorina显示文摘 | Stricker A R Steiger M G Mach R L | | 0，，21： | 1 |
| 16 | Transformation of Trichoderma reesei based on hygromycin B resistance using homologous expression signals显示文摘 | MACH R L SCHINDLER M KUBICEK C P | 1994 | Curr Genet1994，25，6： | 1 |
| 17 | Regulation of transcription of cellulases-and hemicellulases-encoding genes in Aspergillus niger and Hypocrea jecorina(Trichoderma reesei)显示文摘 | Stricker A R Mach R L de Graaff L H | | 0，，02： | 1 |
| 18 | Expression of two major chitinase genes of Trichoderma atrovirideis triggered by different regulatory signals显示文摘 | Peterbauer C K Payer K | 1999 | Applied Environmental Microbiology1999，65，5： | 1 |
| 19 | Regulation of transcription ofcellulases- and hemicellulases-encoding genes in Aspergillus niger and Hypocrea jecorina ( Trichoderma reesei )显示文摘 | STRICKER A R MACH R L DE GRAAFF L H | 2008 | Appl Mierobioi Blot2008，78，2： | 1 |
| 20 | Phylogenetic relationship of Fusarium langsethiae to Fusarium poae and Fusarium sporotrichioides as inferred by IGS,ITS,β-tubulin sequences and UP-PCR hybridization analysis显示文摘 | Yli-Mattila T Mach R L Alekhina I A | 2004 | International Journal of Food Microbiology2004，95，3： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员