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| 1 | Cloning of first abc transporter encoding gene from Trichoderma spp. and its expression during stress and mycoparasitism显示文摘Trichoderma in its natural environment competes for nutrient uptake and is required to protect itself from adverse natural toxic compounds, such as those produced by plants and other microbes in the soil community, or synthetic toxic compounds released human activity. One of the most important metabolic pathways for drug resistance and substrate uptake, both in prokaryotes and eukaryotes, is ATP dependent. The role of ABC transporter proteins in the biology of Trichoderma is still not known. We present the cloning of the first four ABC transporter genes (TABC1, TABC2, TABC3, TABC4 ) in Trichoderma, and in particular T. atroviride P1, and the characterization of TABC2 The complete sequence of this gene is 6535 bp, which includes a promoter of 1624 bp, a terminator of 642 bp and a coding region of 4264 bp. The promoter contains many of the potential transcription factor binding sites found in the 5’ upstream region of the ech42 gene of T. atroviride P1. These included: heat shock factors (HSF), a nitrogen-regulating factor (Nit-2), a stress-response element (STRE), a GCR1 elements, and a Cre BP1 motif. Northern analysis and RT-PCR demonstrated that TABC2 is highly expressed when Trichoderma is subjected to nitrogen starvation, grown in the presence of culture filtrates of Botrytis cinerea, Rhizoctonia solani, and Pythium ultimum, or when N-acetylglucosamine is added to the substrate. TABC2 appears to be co-regulated with some CWDE-encoding genes, suggesting that this is the first ABC transporter encoding gene involved in mycoparasitic events. It’s role in the interaction of Trichoderma with fungal hosts or plants is being investigated by targeted gene disruption and overexpression. | Lanzuise S Ruocco M Scala V Catapano L Woo S Ciliento R Ferraioli S Soriente I Vinale F Scala F Del Sorbo G Lorito M | 2004 | 浙江大学学报(农业与生命科学版)2004，30，4： | 2 |
| 2 | Genetic improvement of Trichoderma ability to induce systemic resistance显示文摘The beneficial applications of Trichoderma spp. in agriculture include not only the control of plant pathogens, but also the improvement of plant growth, micronutrient availability, and plant tolerance to abiotic stress. In addition, it has been suggested that these fungi are able to increase plant disease resistance by activating induced systemic resistance (ISR) . The mode of action of these beneficial fungi in the Trichoderma -plant-pathogen interaction are many, complex and not completely understood. Numerous lytic enzymes have been characterized, the encoding genes (ech42 gluc78, nag1 from T. atroviride strain P1) cloned, and their role in biocontrol demonstrated. The corresponding biocontrol-related inducible promoters have been used in a reporter system based on the Aspergillus niger glucose oxidase gene (goxA) to monitor biocontrol activity. Glucose oxidase catalyzes the oxygen-dependent oxidation of D-glucose to D-glucono-1,5-lactone and hydrogen peroxide; this latter compound is known to have an antifungal effect and activate the plant defence cascade, thus increasing resistance to pathogen attack. T. atroviride P1 transformants with various promoters gox were tested as seed coating treatments on bean seeds planted in soil infested with a soilborne fungal pathogen. Successively, the emergent leaves were inoculated with a foliar pathogen to determine the effect of the GOX transformants on biocontrol and resistance to pathogen attack. Inoculations with the P1-GOX transformants not only reduced disease symptoms caused by a soil pathogen, but also the lesions of various foliar pathogens applied far from the Trichoderma colonization, thus activating ISR. A similar approach is being use to genetically improve T. harzianum T22, a rhizosphere competent and commercially marketed strain not transformed yet, by using four different gox gene constructs under the control of constitutive and inducible promoters. Plasmids have been introduced in Trichoderma by protoplasts co-transformation. hygromicin resistant progeny selected, and mitotically stable transformants analysed to confirm the presence of the novel enzyme activity. Progenies are being tested for biocontrol ISR inducing activity. | Ciliento R Woo S L Di Benedetto P Ruocco M Scala F Soriente I Ferraioli S Brunner K Zeilinger S Mach R L Lorito M | 2004 | 浙江大学学报(农业与生命科学版)2004，30，4： | 2 |
| 3 | The predictive value of plasma biomarkers in discharged heart failure patients:the role of plasma BNP显示文摘 | Beltrami M Palazzuoli A Ruocco G | 2016 | Minerva Cardioangiol2016，64，： | 1 |
| 4 | P- atients with cardiorenal syndrome revealed increased neu- rohormonal activity, tubular and myocardial damage com- pared to heart failure patients with preserved renal function 显示文摘 | PALAZZUOLI A RUOCCO G PELLEGRINI M | 2014 | Cardiorenal Med2014，4，34： | 1 |
| 5 | Endothelin 1 is overexpressed in human cirrhotic liver and exerts multiple effects on activated hepatic stellate cells显示文摘 | M Pinzani S Milani R De Franco C Grappone A Caligiuri A Gentilini C Tosti- Guerra M Maggi P Failli C Ruocco P Gentilini | 1996 | Gastroenterology1996，，2： | 1 |
| 6 | Im- munological and clinical aspects of lymphocytic hypophys- itis显示文摘 | DE BELLIS A RUOCCO G BATTAGLIA M | 2008 | ClinSci (Lond)2008，114，6： | 1 |
| 7 | The molecular biology of the interactions between Trichoderma spp,phytopathogenic fungi,and plants显示文摘 | Woo S L Scala F Ruocco M | 2006 | Phytopathology2006，96，： | 1 |
| 8 | Four potato(Solanum tuberosum)ABCG transporters and their expression in response to abiotic factors and phytophthora infestans infection显示文摘 | Ruocco M Ambrosinob P Lanzuise S | 2011 | Journal of Plant Physiol2011，168，18： | 1 |
| 9 | A 3 Ma paleomagnetic record of coastal continental deposits in Argentina显示文摘 | Ruocco M | 1989 | Palaeogeography Palaeoclimatology Palaeoecology1989，72，： | 1 |
| 10 | Treatment of cereal products with a tailored preparation of trichoderma enzymes increases the amount of soluble dietary fiber显示文摘 | NAPOLITANO A LANZUISE S RUOCCO M | | 0，，20： | 1 |
| 11 | Comparison of neutrophil gelatinase- associated li pocalin versus B- type natriuretic peptide and cystatin C to predict early acute kidney injury and outcome in patients with acute heart failure 显示文摘 | Palazzuoli A Ruocco G Pellegrini M | 2015 | Am J Cardiol2015，116，1： | 1 |
| 12 | Fat-storing ce-lls as liver-specific pericytes spatial dynamics of agon-ist-stimulated intracelullar calcium transients显示文摘 | Pinzani M Failli P Ruocco C | 1992 | J Cl-in Invest1992，90，： | 1 |
| 13 | Cloning and functional characterization of BcatrA, a gene encoding an ABC transporter of the plant pathogenic fungus Botryotinia fuckeliana ( Botrytis cinema ) 显示文摘 | DEISORBO Q RUOCCO M SCHOONBEEK H J | 2008 | Mycol Res2008，112，6： | 1 |
| 14 | IKKI betal as a target for treatment of inf- lamnation induced bone loss显示文摘 | Ruocco MG Karin M | 2005 | Ann Rheum Dis2005，64，5： | 1 |
| 15 | 查看详情显示文摘 | Conti C Fratalocchi A Peccianti M Ruocco G Trillo S | | 0，，： | 1 |
| 16 | Treatment of cereal products with a tailored preparation of trichoderma enzymes increases the amount of soluble dietary fiber显示文摘 | NAPOLITANO A LANZUISE S RUOCCO M | 2006 | J Agric Food Chem2006，54，20： | 1 |
| 17 | Treatment ofcereal products with a tailored preparation of Trichodermaenzymes increases the amount of soluble dietary fiber 显示文摘 | Napolitano A Lanzuise S Ruocco M | 2006 | Journal of agricultural and food chemistry2006，54，20： | 1 |
| 18 | Endothelin 1 is overexpressed in human cirrhotic liver and exerts multiple effects on activated hepatic stellate cells显示文摘 | M Pinzani S Milani R De Franco C Grappone A Caligiuri A Gentilini C Tosti- Guerra M Maggi P Failli C Ruocco P Gentilini | 1996 | Gastroenterology1996，，2： | 1 |
| 19 | The role of natriuretic peptides for the diagnosis of left ventricular dysfunction 显示文摘 | Palazzuoli A Beltrami M Ruocco G | 2013 | ScientificWorldJournal2013，2013，78： | 1 |
| 20 | The molecular biology of the interactions between Trichoderma spp,Phytopathogic fungi and Plants显示文摘 | WOO S L SCALA F RUOCCO M | 2006 | Phytopatho12006，96，： | 1 |
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