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| 1 | Trabecular-like Ti-6Al-4V scaffolds for orthopedic: fabrication by selective laser melting and in vitro biocompatibility显示文摘Porous metal scaffolds play an important role in the orthopedic field, due to their wide applications in prostheses implantation. Some previous studies showed that the scaffolds with trabecular bone structure reconstructed via computed tomography had satisfactory biocompatibility. However, the reverse modeling scaffolds were inflexible for customized design. Therefore, a top-down designing biomimetic bone scaffold with favorable mechanical performances and cytocompatibility is urgently demanded for orthopedic implants. An emerging additive manufacturing technique, selective laser melting, was employed to fabricate the trabecular-like porous Ti-6Al-4 V scaffolds with varying irregularities(0.05-0.5) and porosities(48.83%–74.28%) designed through a novel Voronoi-Tessellation based method. Micro-computed tomography and scanning electron microscopy were used to characterize the scaffolds’ morphology.Quasi-static compression tests were performed to evaluate the scaffolds’ mechanical properties. The MG63 cells culture in vitro experiments, including adhesion, proliferation, and differentiation, were conducted to study the cytocompatibility of scaffolds. Compressive tests of scaffolds revealed an apparent elastic modulus range of 1.93–5.24 GPa and an ultimate strength ranging within 44.9–237.5 MPa, which were influenced by irregularity and porosity, and improved by heat treatment. Furthermore, the in vitro assay suggested that the original surface of the SLM-fabricated scaffolds was favorable for osteoblasts adhesion and migration because of micro scale pores and ravines. The trabecular-like porous scaffolds with full irregularity and higher porosity exhibited enhanced cells proliferation and osteoblast differentiation at earlier time, due to their preferable combination of small and large pores with various shapes. This study suggested that selective laser melting-derived Ti-6Al-4 V scaffold with the trabecular-like porous structure designed through Voronoi-Tessellation method, favorable mechanical performance, and good cytocompatibility was a potential biomaterial for orthopedic implants. | Huixin Liang Youwen Yang Deqiao Xie Lan Li Ning Mao Changjiang Wang Zongjun Tian Qing jiang Lida Shen | 2019 | Journal of Materials Science & Technology2019，35，7： | 12 |
| 2 | Interspecies competition for nutrients between Prorocentrum donghaiense Lu and Skeletonema costatum(Grev.) Cleve in mesocosm experiments显示文摘Three mesocosm experiments were conducted in the East China Sea during blooms of Prorocentrum donghaiense Lu in May of 1998,2002 and 2003 to examine the role of nutrients in the formation of these harmful algae blooms(HABs).The results showed that there was interspecies competition between P.donghaiense and Skeletonema costatum(Grev.) Cleve.The cell number of P.donghaiense and S.costatum accounted for about 85% and 11% of the total cell number of phytoplankton respectively at the beginning of the experiment in May 1998.In May 2002,at the beginning of the experiment,the cell number of P.donghaiense accounted for 55%-66% of the total and S.costatum accounted for 32%-42%.The density of P.donghaiense and S.costatum was over 95% and 1%-5% respectively in May 2003.The results of these three mesocosm experiments showed that the dominant species in this specific community varied with different nutrient availability.Under low-phosphate conditions,the dominant species was P.donghaiense,while S.costatum became dominant in phosphate-replete cases.The average growth rate(1.08 d-1) of S.costatum in exponential growth phase was higher than that(0.39 d-1) of P.donghaiense.In the mesocosm,S.costatum predominated in the mesocosm by its rapid growth.When phosphorus was depleted,the cell number of S.costatum dropped rapidly,while P.donghaiense Lu decreased more slowly.The results from mesocosm experiment may explain why:(1) P.donghaiense bloom usually occurs in May,when phosphate concentration is low;(2) the bloom of S.costatum appears in early spring and summer time,when nutrients increase with increasing Changjiang River(Yangtze River) runoff;(3) the bloom of S.costatum is short and that of P.donghaiense Lu can last more than a month in the East China Sea. | ZHU Mingyuan XU Zongjun LI Ruixiang WANG Zongling SHI Xiaoyong | 2009 | Acta Oceanologica Sinica2009，28，1： | 10 |
| 3 | Effects of fumaric acid supplementation on methane production and rumen fermentation in goats fed diets varying in forage and concentrate particle size显示文摘Background: In rumen fermentation, fumaric acid(FA) could competitively utilize hydrogen with methanogenesis to enhance propionate production and suppress methane emission, but both effects were diet-dependent. This study aimed to explore the effects of FA supplementation on methanogenesis and rumen fermentation in goats fed diets varying in forage and concentrate particle size.Methods: Four rumen-cannulated goats were used in a 4 × 4 Latin square design with a 2 × 2 factorial arrangement of treatments: low or high ratio of forage particle size: concentrate particle size(Fps:Cps), without or with FA supplementation(24 g/d). Fps:Cps was higher in the diet with chopped alfalfa hay plus ground corn than in that with ground alfalfa hay plus crushed corn.Results: Both increasing dietary Fps:Cps and FA supplementation shifted ruminal volatile fatty acid(VFA) patterns toward more propionate and less acetate in goats. An interaction between dietary Fps:Cps and FA supplementation was observed for the ratio of acetate to propionate(A:P), which was more predominant when FA was supplemented in the low-Fps:Cps diet. Methane production was reduced by FA, and the reduction was larger in the low-Fps:Cps diet(31.72%) than in the high-Fps:Cps diet(17.91%). Fumaric acid decreased ruminal total VFA concentration and increased ruminal p H. No difference was found in ruminal DM degradation of concentrate or alfalfa hay by dietary Fps:Cps or FA. Goats presented a lower ruminal methanogen abundance with FA supplementation and a higher B. fibrisolvens abundance with high dietary Fps:Cps.Conclusions: Adjusting dietary Fps:Cps is an alternative dietary model for studying diet-dependent effects without changing dietary chemical composition. Fumaric acid supplementation in the low-Fps:Cps diet showed greater responses in methane mitigation and propionate increase. | Zongjun Li Nannan Liu Yangchun Cao Chunjia Jin Fei Li Chuanjiang Cai Junhu Yao | 2018 | Journal of Animal Science and Biotechnology2018，9，2： | 8 |
| 4 | A Chengjiang-type fossil assemblage from the Hongjingshao Formation(Cambrian Stage 3) at Chenggong, Kunming, Yunnan显示文摘A new Chengjiang-type fossil assemblage is reported herein from the lower part of the Hongjingshao Formation at Xiazhuang village of Chenggong,Kunming,Yunnan.The fossil assemblage,named as Xiazhuang fossil assemblage,yields predominantly soft-bodied fossils,including arthropods,brachiopods,priapulids,lobopods and some problematic taxa,with arthropods being the most dominant group.Preservation and composition of the fossil assemblage are very similar to the typical Chengjiang biota,which is preserved in the middle Yu’anshan Formation in the large area of eastern Yunnan.The associated trilobites demonstrate that the soft-bodied fossil assemblage belongs to the late Qiongzhusian in age(Stage 3,Cambrian),suggesting that the Hongjingshao Formation is probably a diachronous lithostratigraphic unit ranging from the upper Qiongzhusian to the lower Canglangpuan stages in eastern Yunnan.The fossil assemblage from the Xiazhuang area fills up the missing link between the typical older Chengjiang biota and the younger Malong and Guanshan biotas,making eastern Yunnan a unique area in the world to reveal the early evolutionary history of animals and palaeocommunity dynamics during the‘‘Cambrian explosion’’. | Han Zeng Fangchen Zhao Zongjun Yin Guoxiang Li Maoyan Zhu | 2014 | Chinese Science Bulletin2014，59，25： | 6 |
| 5 | Use of Probiotics in Aquaculture显示文摘Diseases of cultured animal and environmental pollution are very important problems in aquaculture. The use of probiotics in aquaculture has more advantages than the use of antibiotics and chemicals in aquaculture. This review introduces the use and mechanisms of the probiotics in aquaculture. | 王祥红 Du Zongjun Chen Jixiang Li Yun Ji Weishang Xu Huaishu | 2002 | High Technology Letters2002，8，3： | 6 |
| 6 | Characterization of the effective cellulose degrading strain CTL-6显示文摘An efficient cellulose degrading bacteria exists in the thermophilic wheat straw-degrading community, WDC2. However, this strain cannot be isolated and cultured using conventional separation techniques under strict anaerobic conditions. We successfully isolated a strain of effective cellulose degrading bacteria CTL-6 using a wash, heat shock, and solid-liquid alternating process. Analysis of its properties revealed that, although the community containing the strain CTL-6 grew under aerobic conditions, the purified strain CTL-6 only grew under anaerobic culture conditions. The strain CTL-6 had a striking capability of degrading cellulose (80.9% weight loss after 9 days of culture). The highest efficiency value of the endocellulase (CMCase activity) was 0.404 μmol/(min·mL), cellulose degradation efficiency by CTL-6 was remarkably high at 50-65°C with the highest degradation efficiency observed at 60°C. The 16S rRNA gene sequence analysis indicated that the closest relative to strain CTL-6 belonged to the genus Clostridium thermocellum. Strain CTL-6 was capable of utilizing cellulose, cellobiose, and glucose. Strain CTL-6 also grew with Sorbitol as the sole carbon source, whereas C. thermocellum is unable to do so. | Yucai Lv Xiaofen Wang Ning Li Xiaojuan Wang Masaharu Ishii Yasuo Igarashi Zongjun Cui | 2011 | Journal of Environmental Sciences2011，23，4： | 6 |
| 7 | Dynamics of methanogenesis,ruminal fermentation and fiber digestibility in ruminants following elimination of protozoa: a meta-analysis显示文摘Background: Ruminal microbes are vital to the conversion of lignocellulose-rich plant materials into nutrients for ruminants.Although protozoa play a key role in linking ruminal microbial networks,the contribution of protozoa to rumen fermentation remains controversial; therefore,this meta-analysis was conducted to quantitatively summarize the temporal dynamics of methanogenesis,ruminal volatile fatty acid(VFA) profiles and dietary fiber digestibility in ruminants following the elimination of protozoa(also termed defaunation).A total of 49 studies from 22 publications were evaluated.Results: The results revealed that defaunation reduced methane production and shifted ruminal VFA profiles to consist of more propionate and less acetate and butyrate,but with a reduced total VFA concentration and decreased dietary fiber digestibility.However,these effects were diminished linearly,at different rates,with time during the first few weeks after defaunation,and eventually reached relative stability.The acetate to propionate ratio and methane production were increased at 7 and 11 wk after defaunation,respectively.Conclusions: Elimination of protozoa initially shifted the rumen fermentation toward the production of more propionate and less methane,but eventually toward the production of less propionate and more methane over time. | Zongjun Li Qi Deng Yangfan Liu Tao Yan Fei Li Yangchun Cao Junhu Yao | 2019 | Journal of Animal Science and Biotechnology2019，10，1： | 3 |
| 8 | Brain areas activated by uncertain reward-based decision-making in healthy volunteers显示文摘Reward-based decision-making has been found to activate several brain areas,including the ventrolateral prefrontal lobe,orbitofrontal cortex,anterior cingulate cortex,ventral striatum,and mesolimbic dopaminergic system.In this study,we observed brain areas activated under three degrees of uncertainty in a reward-based decision-making task(certain,risky,and ambiguous).The tasks were presented using a brain function audiovisual stimulation system.We conducted brain scans of15 healthy volunteers using a 3.0T magnetic resonance scanner.We used SPM8 to analyze the location and intensity of activation during the reward-based decision-making task,with respect to the three conditions.We found that the orbitofrontal cortex was activated in the certain reward condition,while the prefrontal cortex,precentral gyrus,occipital visual cortex,inferior parietal lobe,cerebellar posterior lobe,middle temporal gyrus,inferior temporal gyrus,limbic lobe,and midbrain were activated during the‘risk’condition.The prefrontal cortex,temporal pole,inferior temporal gyrus,occipital visual cortex,and cerebellar posterior lobe were activated during ambiguous decision-making.The ventrolateral prefrontal lobe,frontal pole of the prefrontal lobe,orbitofrontal cortex,precentral gyrus,inferior temporal gyrus,fusiform gyrus,supramarginal gyrus,inferior parietal lobule,and cerebellar posterior lobe exhibited greater activation in the‘risk’than in the‘certain’condition(P<0.05).The frontal pole and dorsolateral region of the prefrontal lobe,as well as the cerebellar posterior lobe,showed significantly greater activation in the‘ambiguous’condition compared to the‘risk’condition(P<0.05).The prefrontal lobe,occipital lobe,parietal lobe,temporal lobe,limbic lobe,midbrain,and posterior lobe of the cerebellum were activated during decision-making about uncertain rewards.Thus,we observed different levels and regions of activation for different types of reward processing during decision-making.Specifically,when the degree of reward uncertainty increased,the number of activated brain areas increased,including greater activation of brain areas associated with loss. | Zongjun Guo Juan Chen Shien Liu Yuhuan Li Bo Sun Zhenbo Gao | 2013 | Neural Regeneration Research2013，8，35： | 3 |
| 9 | Modes of genetic adaptations underlying functional innovations in the rumen显示文摘The rumen is the hallmark organ of ruminants and hosts a diverse ecosystem of microorganisms that facilitates efficient digestion of plant fibers.We analyzed 897 transcriptomes from three Cetartiodactyla lineages:ruminants,camels and cetaceans,as well as data from ruminant comparative genomics and functional assays to explore the genetic basis of rumen functional innovations.We identified genes with relatively high expression in the rumen,of which many appeared to be recruited from other tissues.These genes show functional enrichment in ketone body metabolism,regulation of microbial community,and epithelium absorption,which are the most prominent biological processes involved in rumen innovations.Several modes of genetic change underlying rumen functional innovations were uncovered,including coding mutations,genes newly evolved,and changes of regulatory elements.We validated that the key ketogenesis rate-limiting gene(HMGCS2)with five ruminant-specific mutations was under positive selection and exhibits higher synthesis activity than those of other mammals.Two newly evolved genes(LYZ1 and DEFB1)are resistant to Gram-positive bacteria and thereby may regulate microbial community equilibrium.Furthermore,we confirmed that the changes of regulatory elements accounted for the majority of rumen gene recruitment.These results greatly improve our understanding of rumen evolution and organ evo-devo in general. | Xiangyu Pan Yudong Cai Zongjun Li Xianqing Chen Rasmus Heller Nini Wang Yu Wang Chen Zhao Yong Wang Han Xu Songhai Li Ming Li Cunyuan Li Shengwei Hu Hui Li Kun Wang Lei Chen Bin Wei Zhuqing Zheng Weiwei Fu Yue Yang Tingting Zhang Zhuoting Hou Yueyang Yan Xiaoyang Lv Wei Sun Xinyu Li Shisheng Huang Lixiang Liu Shengyong Mao Wenqing Liu Jinlian Hua Zhipeng Li Guojie Zhang Yulin Chen Xihong Wang Qiang Qiu Brian PDalrymple Wen Wang Yu Jiang | 2021 | Science China(Life Sciences)2021，64，1： | 3 |
| 10 | Analysis of oral microorganism diversity in healthy individuals before and after chewing areca nuts using PCR-denatured gradient gel electrophoresis显示文摘To analyze oral microbial diversity in the saliva of 8 healthy individuals before and after chewing areca nuts. Saliva samples were collected before chewing areca nuts, after chewing areca nuts for 5 min and after chewing areca nuts for 30 min. DNA was extracted, and microbial diversity was examined using PCR-denaturing gradient gel electrophoresis(PCR-DGGE). When examining DGGE profiles collectively,the bands associated with Streptococcus and Veillonella were the most intense, making them the most prevalent bacteria. Furthermore, the band intensities did not decrease after chewing areca nuts for 5 or30 min; thus, these bacteria were unaffected. However, when examining some individuals, the band intensities for Streptococcus and Veillonella became more intense after 5 min of chewing and then returned to the pre-chewing level. This difference may be attributed to the mechanical movements of the oral cavity or individual differences. Other bacteria, such as Neisseria, Actinomycetes, and Rothia dentocariosa, were also found to have an increased or decreased prevalence following areca nut-chewing.Since the predominant species that are present following areca nut-chewing include Streptococcus and Veillonella, it would seem likely that these bacteria play an important role in the periodontal diseases associated with areca chewing. | Xiong Xiong Aixiang Hou Shuhan Yi Yichen Guo Ziwei Zhao Zhongkun Wu Huan Cheng Ke Li Zongjun Li Youhua Ren Yuanliang Wang | 2018 | Animal Nutrition2018，，3： | 2 |
| 11 | Extractive desulfuriza-tion of fuel oil using alkylimidazole and its mixture with dialkylphosphate ionic liquids显示文摘 | Nie Yi Li Cunxi Wang Zongjun | 2007 | Ind Eng Chem Res2007，46，15： | 1 |
| 12 | Allelopathic effects of Hemistepta lyrata on germination and growth of wheat, sorghum, cucumber, rape, and radish seeds 显示文摘 | Gao Xingxiang Li Mei Gao Zongjun | 2009 | Weed Biology and Management2009，9，： | 1 |
| 13 | Flavor release from traditional dry-cured pork during oral processing显示文摘The rate of flavor release in the mouth from traditional dry-cured pork was evaluated in this study.It was hypothesized that a slow steady release of flavor would occur on chewing and hydration.To test this,high salt dry-cured pork was chewed and held in oral cavity without swallowing for 4 different chewing stages(B,NC_(30),C_(30),EC).Saliva and food bolus from 16 healthy subjects were collected during oral processing,and analyzed for saliva flow rate,total saliva protein content,saliva pH,conductivity,saliva and sodium,and taste components.Results show that oral processing behavior and salivary release have important effects on flavor release of dry-cured pork.Sodium dodecyl sulfate-polyacrylamide gel electrophoresis(SDS-PAGE)analysis showed that oral processing has a certain effect on the protein composition and concentration in saliva.This study suggests that the interaction between food and saliva that accompanies chewing can lead to significant changes in sensory perception during oral processing. | Xing Tian Zongjun Li Ke Li Zhongqin Wu Rui Ren Haodong Wang Chaoqun Zeng | 2023 | Food Science and Human Wellness2023，12，1： | 1 |
| 14 | Fungal community associated with fermentation and storage of Fuzhuan brick-tea显示文摘 | Aiqing Xu Yuanliang Wang Jieyu Wen Ping Liu Ziyin Liu Zongjun Li | 2011 | International Journal of Food Microbiology2011，，1： | 1 |
| 15 | Effect of amorphous grain boundaries on the magnetic properties of B-rich nanocomposite permanent magnets 显示文摘 | Li Shandong Dai Yaodong Du Youwei Gu B X Tian Zongjun | 2002 | Journal of Alloys and Compounds2002，339，： | 1 |
| 16 | A Solar Radio Spectrometer at 5.2–7.6?GHz显示文摘 | Huirong Ji Qijun Fu Yuying Liu Congling Cheng Zhijun Chen Yihua Yan Leping Zheng Zongjun Ning Chengmin Tan Debang Lao Shuke Li Jiyong Gao Zhiqiang Wang Minhong Yu | 2003 | Solar Physics2003，，2： | 1 |
| 17 | The Effects of Shrimp Gut Probiotic Bacteria on the Shrimp Larvae (Penaeus Chinensis)显示文摘The survival rates of shrimp larvae in different stage are higher than those of control groups when probiotic bacteria strains X4B 1 and X1B 1 which are isolated from gut of adult shrimp are added into the little volume rearing sea water of shrimp larvae. The effects of probiotic bacteria are evaluated by challenge test (pathogenic bacteria Z 3G 2 isolated from disease shrimp larvae in the hatchery of Jimo town) and low salinity stress resistance tests on shrimp larvae, the survival rate and lengths of the shrimp larvae in the experiment are determined. Results indicate that 1. The survival rate, ability of resistant to low salinity, lengths of the delivered shrimp larvae are improved after the strains of probiotic bacteria, X4B 1 or X1B 1, are added into the rearing sea water of hatchery. 2. The addition of the probiotic bacteria could not influence the change of the bacteria number, NH3 N and COD value in the rearing sea water. 3. The probiotic bacteria used in the experiment have many enzymes such as Lipase, Amylase, Gelatinase and Lecithinase. These enzymes may help the probiotic bacteria to digest the food components fed to shrimp larvae and increase the digestive efficiency of post larvae. This may be one of the reasons why these probiotic bacteria are beneficial to the shrimp larvae. | 王祥红 Du Zongjun Li Huirong Li Yun Ji Weishang Xu Huaishu | 2002 | High Technology Letters2002，8，4： | 1 |
| 18 | Reaction of C60^2- with Organic Halides Revisited in DMF: Proton Transfer from Water to RC60^2- and Unexpected Formation of 1,2-Dihydro fullerenes显示文摘 | Yang Weiwei Li Zongjun Gao Xiang | 2010 | J Org Chem2010，75，： | 1 |
| 19 | Long-term and combined effects of N-[2-(nitrooxy)ethyl]-3-pyridinecarboxamide and fumaric acid on methane production,rumen fermentation, and lactation performance in dairy goats显示文摘Background:In recent years,nitrooxy compounds have been identified as promising inhibitors of methanogenesis in ruminants.However,when animals receive a nitrooxy compound,a high portion of the spared hydrogen is eructated as gas,which partly offsets the energy savings of CH4mitigation.The objective of the present study was to evaluate the long-term and combined effects of supplementation with N-[2-(nitrooxy)ethyl]-3-pyridinecarboxamide(NPD),a methanogenesis inhibitor,and fumaric acid(FUM),a hydrogen sink,on enteric CH4production,rumen fermentation,bacterial populations,apparent nutrient digestibility,and lactation performance of dairy goats.Results:Twenty-four primiparous dairy goats were used in a randomized complete block design with a 2×2factorial arrangement of treatments:supplementation without or with FUM(32 g/d)or NPD(0.5 g/d).All samples were collected every 3 weeks during a 12-week feeding experiment.Both FUM and NPD supplementation persistently inhibited CH4yield(L/kg DMI,by 18.8%and 18.1%,respectively)without negative influence on DMI or apparent nutrient digestibility.When supplemented in combination,no additive CH4suppression was observed.FUM showed greater responses in increasing the molar proportion of propionate when supplemented with NPD than supplemented alone(by 10.2%vs.4.4%).The rumen microbiota structure in the animals receiving FUM was different from that of the other animals,particularly changed the structure of phylum Firmicutes.Daily milk production and serum total antioxidant capacity were improved by NPD,but the contents of milk fat and protein were decreased,probably due to the bioactivity of absorbed NPD on body metabolism.Conclusions:Supplementing NPD and FUM in combination is a promising way to persistently inhibit CH4emissions with a higher rumen propionate proportion.However,the side effects of this nitrooxy compound on animals and its residues in animal products need further evaluation before it can be used as an animal feed additive. | Zongjun Li Xinjian Lei Xiaoxu Chen Qingyan Yin Jing Shen Junhu Yao | 2022 | Journal of Animal Science and Biotechnology2022，13，3： | 1 |
| 20 | Extreme-Point Symmetric Mode Decomposition Method for Data Analysis 显示文摘 | JinLiang Wang Zongjun Li | 2013 | Advances in Adaptive Data Analysis2013，5，13： | 1 |
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