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| 1 | Analysis of status and countermeasures of cancer incidence and mortality in China显示文摘Cancer is the leading cause of human deaths in the world and produces serious economic burdens. On September 12, 2018, the academic journal A Cancer Journal for Clinicians published an article about the latest statistics of cancers worldwide, which provided a status report on the global burden of 36 cancers in 185 countries worldwide. Cancer has also become a serious public health problem in China and caused more and more attention of the government and people in recent years. This review analyzes the incidence, mortality and prevalent trend of cancers in China, discusses the reasons behind this status, and reviews the potential countermeasures for cancer prevention and control in China. | Chunchun Wu Mengna Li Hanbing Meng Yukun Liu Weihong Niu Yao Zhou Ran Zhao Yumei Duan Zhaoyang Zeng Xiaoling Li Guiyuan Li Wei Xiong Ming Zhou | 2019 | Science China(Life Sciences)2019，62，5： | 175 |
| 2 | Microwave Exposure Impairs Synaptic Plasticity in the Rat Hippocampus and PC12 Cells through Over-activation of the NMDA Receptor Signaling Pathway显示文摘Objective The aim of this study is to investigate whether microwave exposure would affect the N-methyl-D-aspartate receptor(NMDAR) signaling pathway to establish whether this plays a role in synaptic plasticity impairment. Methods 48 male Wistar rats were exposed to 30 m W/cm2 microwave for 10 min every other day for three times. Hippocampal structure was observed through H&E staining and transmission electron microscope. PC12 cells were exposed to 30 m W/cm2 microwave for 5 min and the synapse morphology was visualized with scanning electron microscope and atomic force microscope. The release of amino acid neurotransmitters and calcium influx were detected. The expressions of several key NMDAR signaling molecules were evaluated. Results Microwave exposure caused injury in rat hippocampal structure and PC12 cells, especially the structure and quantity of synapses. The ratio of glutamic acid and gamma-aminobutyric acid neurotransmitters was increased and the intracellular calcium level was elevated in PC12 cells. A significant change in NMDAR subunits(NR1, NR2 A, and NR2B) and related signaling molecules(Ca2+/calmodulin-dependent kinase II gamma and phosphorylated c AMP-response element binding protein) were examined. Conclusion 30 m W/cm2 microwave exposure resulted in alterations of synaptic structure, amino acid neurotransmitter release and calcium influx. NMDAR signaling molecules were closely associated with impaired synaptic plasticity. | XIONG Lu SUN Cheng Feng ZHANG Jing GAO Ya Bing WANG Li Feng ZUO Hong Yan WANG Shui Ming ZHOU Hong Mei XU Xin Ping DONG Ji YAO Bin Wei ZHAO Li PENG Rui Yun | 2015 | Biomedical and Environmental Sciences2015，28，1： | 16 |
| 3 | Persistence of side population cells with high drug efflux capacity in pancreatic cancer显示文摘AIM: To investigate the persistence of side population (SP) cells in pancreatic cancer and their role and mechanism in the drug resistance. METHODS: The presentation of side population cells in pancreatic cancer cell line PANC-1 and its proportion change when cultured with Gemcitabine, was detected by Hoechst 33342 staining and FACS analysis. The expression of ABCB1 and ABCG2 was detected by real- time PCR in either SP cells or non-SP cells. RESULTS: SP cells do exist in PANC-1, with a median of 3.3% and a range of 2.1-8.7%. After cultured with Gemcitabine for 3 d, the proportion of SP cells increased significantly (3.8% ± 1.9%, 10.7% ± 3.7%, t = 4.616, P = 0.001 < 0.05). ABCB1 and ABCG2 expressed at higher concentrations in SP as compared with non-SP cells (ABCB1: 1.15 ± 0.72, 5.82 ± 1.16, t = 10.839, P = 0.000 < 0.05; ABCG2: 1.16 ± 0.75, 5.48 ± 0.94, t = 11.305, P = 0.000 < 0.05), which may contribute to the efflux of fluorescent staining and drug resistance. CONCLUSION: SP cells with inherently high resistance to chemotherapeutic agents do exist in pancreatic cancers, which may be candidate cancer stem cells contributing to the relapse of the tumor. | Jing Zhou Chun-You Wang Tao Liu Bin Wu Feng Zhou Jiong-Xin Xiong He-Shui Wu Jing Tao Gang Zhao Ming Yang Shan-Miao Gou | 2008 | World Journal of Gastroenterology2008，14，6： | 13 |
| 4 | Monitoring disease progression and treatment efficacy with circulating tumor cells in esophageal squamous cell carcinoma: A case report显示文摘This study investigated whether changes in circulating tumor cell(CTC) numbers reflect tumor progression and treatment efficacy in esophageal squamous cell carcinoma(ESCC). A 47-year-old male patient with ESCC is presented in this case study. The patient was evaluated for a series of serum tumor markers and subjected to radiological examinations before and after surgery and during follow-up over the course of five years. In addition, the CTCs in 7.5 m L of peripheral blood were enriched by magneticactivated cell sorting negative selection and identified by immunofluorescence staining. Serum tumor markers remained within normal ranges and were discordant with imaging scans during the follow-up. Initially, one CTC was detected in the peripheral blood sample, and 14 were observed seven days after the operation. After 12 wk, subcutaneous metastases and bone metastases occurred, and the number of CTCs increased to 84. After 48 wk, lung metastases were noted, and the CTC level was 21. At 104 wk, the number of CTCs was 14, and disease recurrence was detected by positron emission tomography-computed tomography. The CTC counts were in accord with the imaging studies at several time points. The additional information provided by CTC enumeration could thus facilitate monitoring of disease status and treatment efficacy and provide support for treatment decisions. | Yuan-Yuan Qiao Kai-Xuan Lin Ze Zhang Da-Jin Zhang Cheng-He Shi Ming Xiong Xiu-Hua Qu Xiao-Hang Zhao | 2015 | World Journal of Gastroenterology2015，21，25： | 11 |
| 5 | AduoLa Fuzhenglin Down-regulates Microwave-induced Expression of β_1-adrenergic Receptor and Muscarinic Type 2 Acetylcholine Receptor in Myocardial Cells of Rats显示文摘This paper is aimed to study the effect of ADL on expression ofβ1-AR and M2-AchR in myocardial cells of rats exposed to microwave radiation.Immunohistochemistry,Western blot and image analysis were used to detect the expression ofβ1-AR and M2-AchR in myocardial cells at 7 and 14 | ZHANG Jing PENG Rui Yun GAO Ya Bing WANG Shui Ming YANG Lei Lei ZHAO Li DONG Ji YAO Bin Wei CHANG Gong Min XIONG Lu | 2014 | Biomedical and Environmental Sciences2014，27，3： | 9 |
| 6 | Malnutrition and Quality of Life in Chinese Cancer Patients: a Clinical Study of 23,994 Subjects显示文摘Background Malnutrition is common in patients with cancer,and can negatively impact their quality of life(QoL)and even survival.However,there is currently no large data available on the prevalence of malnutrition in Chinese cancer patients.This study evaluated the prevalence of malnutrition and the QoL of Chinese patients with locoregional,recurrent or metastatic cancer.Methods We conducted a nationwide observational,multi-center,hospital-based cross-sectional study within the Chinese Society of Nutritional Oncology(CSNO)Network.All of the patients were diagnosed with one of the following 18 different types of malignant tumors:lung cancer,gastric cancer,liver cancer,colorectal cancer,breast cancer,esophageal cancer,cervical cancer,endometrial cancer,nasopharyngeal carcinoma,malignant lymphoma,leukemia,pancreatic cancer,ovarian cancer,prostate cancer,bladder cancer,brain cancer,biliary tract malignant tumors or gastrointestinal stromal tumors.These patients were enrolled from 72 hospitals located in different regions of China.The patients’nutritional status was evaluated based on the body mass index(BMI),loss of bodyweight,laboratory measurements and patient generated-subjective global assessment(PG-SGA)scores.The cancer patients’physical status and QoL were assessed by the Karnofsky Performance Status(KPS)questionnaire and the European Organization for Research and Treatment of Cancer(EORTC)QLQ-C30 questionnaire,respectively.Results From December 2013 to April 2016,23,994 patients hospitalized for cancer treatment(such as surgery,chemotherapy or radiotherapy)were enrolled in the study.The patients included 12,494(52.9%)males and 11,124(47.1%)females.The mean age was 55.8±13.7 years.The proportions of patients in cancer stagesⅠ,Ⅱ,Ⅲ,Ⅳand uncertain were 11.5%,20.3%,27.5%,30.2%and 10.5%,respectively.Among the 23,994 inpatients,the proportions of patients who were underweight(BMI<18.5 kg/m2),normal(18.5 kg/m2 | Hong Xia Xu Chun Hua Song Zhen Ming Fu Chang Wang Zeng Qing Guo Yuan Lin Ying Ying Shi Wen Hu Yi Ba Su Yi Li Zeng Ning Li Kun Hua Wang Jing Wu Ying He Jia Jun Yang Cong Hua Xie Fu Xiang Zhou Xin Xia Song Gong Yan Chen Wen Jun Ma Su Xia Luo Zi Hua Chen Ming Hua Cong Hu Ma Chun Ling Zhou Wei Wang Qi Luo Yong Mei Shi Yu Mei Qi Hai Ping Jiang Wen Xian Guan Jun Qiang Chen Jia Xin Chen Yu Fang Lan Zhou Yong Dong Feng Rong Shao Tan Tao Li Jun Wen Ou Qing Chuan Zhao Jian Xiong Wu Min Weng Qing Hua Yao Jiu Wei Cui Wei Li Han Ping Shi the Investigation on Nutrition Status and Clinical Outcome of Common Cancers(INSCOC)Group | 2021 | Journal of Nutritional Oncology2021，6，1： | 8 |
| 7 | ACAT1 regulates the dynamics of free cholesterols in plasma membrane which leads to the APP-a-processing alteration显示文摘 | Ming Zhu Xiaonan Zhao Jia Chen Jiajia Xu Guangjing Hu Dongqing Guo Qin Li Xiaowei Zhang Catherine C.Y. Chang Baoliang Song Ying Xiong Tayuan Chang Boliang Li | 2015 | Acta Biochimica et Biophysica Sinica2015，47，12： | 7 |
| 8 | 牛至不同部位所得精油的化学成分及抗氧化活性研究(英文)显示文摘目的:本研究试图证明湖北产牛至不同部位所得精油存在化学成分和抗氧化性的差异,并为开发牛至药材新的药用部位提供实验依据及参考。创新点:首次对湖北产牛至的'地上部分'和'地下部分'进行了化学成分和抗氧化活性的对比研究,结果表明'地下部分'同样是有效的药用部位。方法:采用气相色谱-质谱联用法(GC-MS)对牛至不同部位(花-叶、茎、根)所得精油的化学成分进行比较与分析(附表1),同时结合二苯三硝基苯肼(DPPH)自由基清除率实验和铁氰化钾总还原能力实验考察了各部位精油的抗氧化性(图1、图2和表1),为牛至精油抗氧化活性的研究进行了新的尝试。结论:湖北产牛至药材的'地下部分'(根)与'地上部分'(花-叶)的精油具有类似的抗氧化作用,具有一定的药用价值。牛至精油在作为抗氧剂使用时,'地下部分'(根)不应被忽略,而应适当保留或开发。 | Fei HAN Guang-qiang MA Ming YANG Li YAN Wei XIONG Ji-cheng SHU Zhi-dong ZHAO Han-lin XU | 2017 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2017，18，1： | 7 |
| 9 | Validity of the Chinese Version of the Patient-Generated Subjective Global Assessment (PG-SGA) in Lung Cancer Patients显示文摘The prevalence of malnutrition is high among oncology patients in China. Although the Patient- Generated Subjective Global Assessment (PG-SGA) is widely recognized as an important nutritional assessment tool, it has not been validated for Chinese cancer patients. The purpose of this study was to conduct an analysis of nutrition screening and assessment data to validate the Chinese version of the PG-SGA in Chinese patients with lung cancer. This was an observational, cross-sectional study of 2,000 consecutive adult patients with lung cancer treated at several tertiary hospitals in China. Anthropometric and patient descriptive data were collected. The PG-SGA generated a score for the nutritional risk and a global rating for the nutritional status. The internal consistency, external consistency, and construct validity were evaluated, and a known-groups comparison and principal component analyses were conducted to evaluate the reliability and validity of the PG-SGA. The known-groups comparison demonstrated the ability of the PG-SGA to differentiate lung cancer patients of different nutritional and body mass index (BMI) groups. A confirmatory factor analysis supported the original four-factor structure of the PG-SGA. In terms of the internal and external consistency, the PG-SGA demonstrated intra-class correlation coefficients (ICC) up to 0.987. Alternative form validity was also demonstrated between Box 4 of the PG-SGA and the Karnofsky Performance Status score, with excellent external consistency (r = 0.972, P < 0.001). The construct validity was supported, and selected questionnaire dimensions were evident in the principal component analysis. Significant Spearman correlations (P < 0.001) were demonstrated. The Chinese version of the PG-SGA demonstrated significant reliability and sufficient exploratory properties to support its validity. It seems to be a valid tool that can be used to access the nutritional status of Chinese lung cancer patients. The validity of the PG-SGA in Chinese cancer patients warrants further investigation in other cancer types. | Zhen Ming Fu Hong Xia Xu Chun Hua Song Wei Li Zeng Qing Guo Yuan Lin Ying Ying Shi Wen Hu Yi Ba Su Yi Li Zeng Ning Li Kun Hua Wang Jing Wu Ying He Jia Jun Yang Cong Hua Xie Xin Xia Song Gong Yan Chen Wen Jun Ma Su Xia Luo Zi Hua Chen Ming Hua Cong Hu Ma Chun Ling Zhou Wei Wang Qi Luo Yong Mei Shi Yu Mei Qi Hai Ping Jiang Wen Xian Guan Jun Qiang Chen Jia Xin Chen Yu Fang Lan Zhou Yong Dong Feng Rong Shao Tan Tao Li Jun Wen Ou Qing Chuan Zhao Jian Xiong Wu Chang Wang Han Ping Shi The Investigation on Nutrition Status and Clinical Outcome of Common Cancers (INSCOC) Group | 2016 | Journal of Nutritional Oncology2016，1，1： | 7 |
| 10 | Lung-protective Ventilation in Patients with Brain Injury: A Multicenter Cross-sectional Study and Questionnaire Survey in China显示文摘 | Xu-Ying Luo Ying-Hong Hu Xiang-Yuan Cao Yan Kang Li-Ping Liu Shou-Hong Wang Rong-Guo Yu Xiang-You Yu Xia Zhang Bao-Shan Li Zeng-Xiang Ma Yi-Bing Weng Heng Zhang De-Chang Chen Wei Chen Wen-Jin Chen Xiu-Mei Chen Bin Du Mei-Li Duan Jin Hu Yun-Feng Hoang Gui-Jun Jia Li-Hong Li Yu-Min Liang Bing-Yu Qin Xian-Dong Wang Jian Xiong Li-Mei Yan Zheng-Ping Yang Chen-Ming Dong Dong-Xin Wang Qing-Yuan Zhan Shuang-Lin FU Lin Zhao Qi-Bing Huang Ying-Guang Xie Xiao-Bo Huang Guo-Bin Zhang Wang-Bin Xu Yuan Xu YaLing Liu He-Ling Zhao Rong-Qing Sun Ming Sun Qing-Hong Cheng Xin Qu Xiao-Feng Yang Ming Xu Zhong-Hua Shi Han Chen Xuan He Yan-Lin Yang Guang-Qiang Chen Xiu-Mei Sun Jian-Xin Zhou | 2016 | Chinese Medical Journal2016，，14： | 6 |
| 11 | A draft sequence of the rice (Oryza sativa ssp. indica) genome显示文摘The sequence of the rice genome holds fundamental information for its biology, including physiology, genetics, development, and evolution, as well as information on many beneficial phenotypes of economic significance. Using a 'whole genome shotgun' approach, we have pro-duced a draft rice genome sequence of Oryza sativa ssp. in-dica, the major crop rice subspecies in China and many other regions of Asia. The draft genome sequence is constructed from over 4.3 million successful sequencing traces with an accumulative total length of 2214.9 Mb. The initial assembly of the non-redundant sequences reached 409.76 Mb in length, based on 3.30 million successful sequencing traces with a total length of 1797.4 Mb from an indica variant cultivar 93-11, giving an estimated coverage of 95.29% of the rice genome with an average base accuracy of higher than 99%. The coverage of the draft sequence, the randomness of the sequence distribution, and the consistency of BIG-ASSEM-BLER, a custom-designed software package | YU Jun, HU Songnian, WANG Jun,LI Songgang WONG Ka-Shu Gane, LIU Bin,DENG Yajun, DAI Li, ZHOU Yan,ZHANG Xiuqing, CAO Mengliang, LIU Jing,SUN Jiandong , TANG Jiabin, CHEN Yanjiong,HUANG Xiaobing, LIN Wei, YE Chen, TONG Wei,CONG Lijuan, GENG Jianing, HAN Yujun, LI Lin,LI Wei, HU Guangqiang, HUANG Xiangang,LI Wenjie, LI Jian, LIU Zhanwei, LI Long,LIU Jianping, Ql Qiuhui, LIU Jinsong, LI Li,WANG Xuegang, LU Hong, WU Tingling,ZHU Miao, Nl Peixiang, HAN Hua, DONG Wei,REN Xiaoyu, FENG Xiaoli, GUI Peng,LI Xianran, WANG Hao, XU Xin, ZHAI Wenxue,XU Zhao, ZHANG Jinsong, HE Sijie,ZHANG Jianguo, XU Jichen, ZHANG Kunlin,ZHENG Xianwu, DONG Jianhai, ZENG Wanyong,TAO Lin, CHEN Xuewei, HE Jun, LIU Daofeng,TIAN Wei, TIAN Chaoguang, XIA Hongai,LI Gang, GAO Hui, LI Ping, CHEN Wei ,WANG Xudong, ZHANG Yong, HU Jianfei,WANG Jing, LIU Song, YANG Jian,ZHANG Guangyu, XIONG Yuqing, LI Zhijie,MAO Long, ZHOU Chengshu, ZHU Zhen,CHEN Runsheng, HAO Bailin,ZHENG Weimou, CHEN Shouyi, QUO Wei,LI Guojie, LIU Siqi, HUANG Guyang,TAO Ming, WANG Jian, ZHU Lihuang,YUAN Longping& YANG HuanmingBeijing Genomics Institute/Center of Genomics & Bioinformatics, Chinese Academy of Sciences, Beijing 101300, China Hangzhou Genomics Institute/Institute of Bioinformatics of Zhejiang University/Key Laboratory of Bioinformatics of Zhejiang Province, Hangzhou 310007, China Institute of Genetics, Chinese Academy of Sciences, Beijing 100101, China National Hybrid Rice R & D Center, Changsha 410125, China Laboratory of Bioinformatics, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China College of Life Sciences, Peking University, Beijing 100871, China Institute of Theoretical Physics, Chinese Academy of Sciences, Beijing 1Q0080, China Digital China Ltd., Beijing 100080, China Institute of Computing Technology, Chinese Academy of Sciences, Beijing 100080, China Medical College, Xi’an Jiaotong University, Xi’an 710061, ChinaThese authors contributed equally to this work.Corresponding author.Corresponden | 2001 | Chinese Science Bulletin2001，46，23： | 6 |
| 12 | Analysis of Specific Binding and Subcellular Localization of Wheat ERF Transcription Factor W17显示文摘The study aims to detect the subcellular localization of ERF (ethylene-responsive element binding factor) transcription factor W17 protein, the interaction between W17 and cis-acting regulatory elements GCC-box and DRE in vitro, the binding and transactivating ability in vivo, and the role of W17 in higher plant stress-signal pathway. Recombinant plasmid W17/163hGFP was introduced into onion epidermal cells by the particle bombardment method with a PDS1000/He. Transformed cells were incubated for 24 h at 22°C in the dark and green fluorescence was monitored under a confocal microscope. The gene W17 was fused N-terminus of GST (glutathione-S-transferase) in prokaryotic expression vector pGEX-4T-1 and then transformed into E. coli strain BL21 (DE3). IPTG (0.5 mmol L-1) was added to induce the expression of recombinant GST/W17 for 3 h. The fused proteins were purified by GST purification columns, and then subjected to gel retardation assay with a 32P-labeled GCC or DRE sequence. The different reporter and effector plasmids were introduced into tobacco leaves through agroinfiltration, then transformed leaves stained by X-Gluc, faded with 75% alcohol and monitored under a Stereozooming microscope. The GFP fused with W17 protein was localized in the nuclei; SDS-PAGE assay demonstrated that the fused protein GST/W17 could be induced and purified with molecular weight at around 42.2 kD under the induction of IPTG. Purified fused protein was able to specifically bind to both the wild-type GCC-box and DRE element, but had no interaction with either the mutant DRE or GCC-box; W17 protein can bind to GCC-box and transactive downstream GUS gene in vivo. W17 can localize into the nuclei, and it may be involved not only in biotic stresses controlled by GCC-box, but also in abiotic stresses (e.g., salt-) induced signaling pathway. | ZHAO Yun-xiang LIU Pei XU Zhao-shi CHEN Ming LI Lian-cheng CHEN Yao-feng XIONG Xiang-jin MA You-zhi | 2008 | Agricultural Sciences in China2008，7，6： | 5 |
| 13 | Validity of the Chinese Version of the Patient-Generated Subjective Global Assessment (PG-SGA) in Gastric Cancer Patients显示文摘Purpose Malnutrition is severe among gastric cancer patients in China. While the Patient-Generated Subjective Global Assessment (PG-SGA) is a widely used nutritional assessment tool, the validation for Chinese patients with gastric cancer has not been performed yet. The aim of this research is to validate the Chinese version of the PGSGA in Chinese gastric cancer patients by conducting analyses of nutrition screening and assessment data. Methods Two thousand consecutive adult patients with gastric cancer were included in this observational, cross-sectional study in several tertiary hospitals in China. A survey was made among those patients, which included several questionnaires and some anthropometric parameters. We calculated the parameters to validate the use of PG-SGA categorizing the nutritional status of gastric cancer patients. Then we performed statistical analysis to evaluate the internal consistency, external consistency, and construct validity. Results This study verified the ability of the PG-SGA to differentiate gastric cancer patients into different nutritional groups. The confirmatory factor analysis verified the original four-factor structure of the PG-SGA. The internal and external consistency was measured by a concordance analysis which showed an intraclass correlation coefficients (ICC) of 0.987. The correlations of the Box 4 score with the Karnofsky performance status score demonstrated alternative form validity with good external consistency (Pearson, r = 0.643, P < 0.001). All three anthropometric dimensions of the PG-SGA were supported by principal component analysis. Spearman correlations (P < 0.001) were significant in the majority of tests. Conclusion The Chinese version of the PG-SGA is a valid tool to access the patients’ nutritional status for Chinese gastric patients. | Zhen Ming Fu Hong Xia Xu Chun Hua Song Wei Li Zeng Qing Guo Yuan Lin Ying Ying Shi Wen Hu Yi Ba Su Yi Li Zeng Ning Li Kun Hua Wang Jing Wu Ying He Jia Jun Yang Cong Hua Xie Xin Xia Song Gong Yan Chen Wen Jun Ma Su Xia Luo Zi Hua Chen Ming Hua Cong Hu Ma Chun Ling Zhou Wei Wang Qi Luo Yong Mei Shi Yu Mei Qi Hai Ping Jiang Wen Xian Guan Jun Qiang Chen Jia Xin Chen Yu Fang Lan Zhou Yong Dong Feng Rong Shao Tan Tao Li Jun Wen Ou Qing Chuan Zhao Jian Xiong Wu Chang Wang Han Ping Shi The Investigation on Nutrition Status and Clinical Outcome of Common Cancers (INSCOC) Group | 2018 | Journal of Nutritional Oncology2018，3，4： | 5 |
| 14 | RNA secondary structures located in the interchromosomal region of human ACAT1 chimeric mRNA are required to produce the 56-kDa isoform显示文摘我们以前报导了人的 ACAT1 基因通过从染色体抄录的二不连续的 RNA 处理的 interchromosomal 生产妄想的 mRNA 1 和 7。妄想的 mRNA 把 AUG1397-1399 和 GGC1274-1276 用作翻译开始 codons 分别地,与在人的房间是确实地在场的后者生产正常 50-kDa ACAT1 和新奇酶地活跃的 56-kDa isoform,包括人的导出单核白血球的巨噬细胞。在这个工作,我们报导位于 GGC1274-1276 codon 的附近的 RNA 第二等的结构为 56-kDa isoform 的生产被要求。三预言的茎环(nt 1255-1268, 1286-1342 和 1355-1384 ) 的效果被 transfecting 表示 plasmids 个别地测试进包含的房间野类型,删除或变异的茎环序列连接了到一个部分 ACAT1 8 月开的读物框架(ORF ) 或到另外的基因的 ORF。表示模式被西方的污点分析监视。我们发现从染色体 7 的在上游的 stem-loop1255-1268 和从染色体 1 的下游的 stem-loop1286-1342 为 56-kDa isoform 的生产被需要,而从染色体 1 的最后 stem-loop1355-1384 是非必需的。用有一个稳定的发卡的 monocistronic 和 bicistronic 向量的实验的结果证明从 GGC1274-1276 codon 的那翻译开始被一个内部核糖体入口地点(怒火) 调停。进一步的实验表明从 GGC1274-1276 codon 的那翻译开始要求在上游的组成 AU 的 RNA 第二等的结构和下游地 GC 富有的结构。这个机械学的工作为人的 ACAT1 抄本的妄想的性质的生物意义提供进一步的支持。 | Jia Chen Xiao-Nan Zhao Li Yang Guang-Jing Hu Ming Lu Ying Xiong Xin-Ying Yang Catherine CY Chang Bao-Liang Song Ta-Yuan Chang Bo-Liang Li | 2008 | Cell Research2008，18，9： | 5 |
| 15 | Production of ACAT1 56-kDa isoform in human cells via trans-splicing involving the ampicillin resistance gene显示文摘拼接 Trans,包含劈开并且二个分开的抄本加入的一个过程,能在优核质扩展 transcriptome 和 proteome。拼接 trans 产生的妄想的 RNA 逐渐地在文学被描述。在自然环境和人的肠的抗菌素抵抗基因的普遍存在为公共健康正在成为重要挑战。某些抗菌素抵抗基因,例如氨比西林抵抗基因(安培 r ), 经常在 recombinant plasmids 被使用。直到现在,拼接 trans 包含 recombinant 导出 plasmid 的外长的抄本和内长的细胞的 RNA 没被报导。Acyl-CoA:cholesterol acyltransferase (ACAT1 ) 1 是涉及细胞的胆固醇动态平衡的关键酶。4.3-kb 人 ACAT1 妄想的 mRNA 能与不同酶的活动生产 50-kDa 和 56-kDa isoforms。这里,我们证明人的 ACAT1 56-kDa isoform 从通过安培 r ( asAmp )现在在普通安培 r -plasmids 和 4.3-kb 内长的 ACAT1 妄想的 mRNA ,它大概通过 interchromosomal 拼接trans 的一个优先的事件被处理。惊人地,与一个在上游的重新结合的神秘倡导者一起包含 asAmp 的 DNA 碎片和相应外长的 asAmp 抄本在人的房间被检测了。我们的调查结果使人的 ACAT1 56-kDa isoform 生产的机制清楚些,揭示一个外长内长的拼接 trans 系统,在 recombinant,导出 plasmid 的外长的抄本在人的房间与内长的细胞的 RNA 被连接,并且建议外长的 DNA 可能影响在 DNA 和 RNA 层次的人的基因表示。 | Guang-Jing Hu Jia Chen Xiao-Nan Zhao Jia-JiaXu Dong-Qing Guo Ming Lu Ming Zhu Ying Xiong Qin Li Catherine CY Chang Bao-Liang Song Ta-Yuan Chang Bo-Liang Li | 2013 | Cell Research2013，23，8： | 4 |
| 16 | Extension Protocol for the Investigation on Nutrition Status and Clinical Outcome of Patients with Common Cancers in China(INSCOC)Study:2021 update显示文摘Background Malnutrition is common in patients with cancer,and this adversely affects the survival and quality of life of patients.Chinese Society for Nutritional Oncology issued a multi-center,large-scale,long-term follow-up prospective study,the Investigation on Nutrition Status and Clinical Outcome of Patients with Common Cancers in China(INSCOC study)since 2013.This is an extension to the previous 2013-2020 study protocol.This study still sought to:①address the prognostic impact of nutritional factors and quality of life on cancer patient survival;②describe the overall and cancer-specific incidence and/or distribution of malnutrition and different measurements of patient quality of life.Methods and study design This is an observational,multi-centered,hospital-based prospective cohort study.Data collection will be performed at baseline(within 48 hours after patient admission),during the hospital stay and 30 days after hospital admission.Follow-up will be conducted for 1-20 years after enrollment.The primary outcome will be the all-cause mortality/overall survival,and secondary outcomes will be the length of hospital stay and costs of hospitalization.Study factors will include demographic characteristics,tumor characteristics,information about chronic diseases,hematological measurements(e.g.,red blood cell count,total lymphocyte counts,hemoglobin,albumin,prealbumin,creatinine,C-reactive protein,IL-6),anthropometric measurements(e.g.,height,weight,arm circumference,arm muscle circumference,triceps skinfold thickness,and waist circumference),body composition parameters,PG-SGA scores,quality of life(as indicated by the QLQ-C30 questionnaire),muscle mass(as indicated by the calf circumference),muscle strength(as indicated by the handgrip strength),muscle function(as indicated by the six-meter walking speed test)and physical status assessments(as indicated by the Karnofsky Performance Status scores).This clinical study protocol was approved by local Ethics Committees of all the participating hospitals.Written informed consent is required for each subject included.Discussion This multi-center,large-scale,long-term follow up prospective study will help improve the diagnosis of malnutrition in cancer patients and identify the risk factors associated with adverse clinical outcomes.The anticipated results of this study will highlight the need for a truly scientific appraisal of nutrition therapy in Chinese oncology populations,and finally help treat the potentially reversible elements of malnutrition in cancer patients to improve their clinical outcomes in the future. | Hong Xia Xu Chun Hua Song Liang Yu Yin Chang Wang Zhen Ming Fu Zeng Qing Guo Yuan Lin Ying Ying Shi Wen Hu Yi Ba Su Yi Li Zeng Ning Li Kun Hua Wang Jing Wu Ying He Jia Jun Yang Cong Hua Xie Fu Xiang Zhou Xin Xia Song Gong Yan Chen Wen Jun Ma Su Xia Luo Zi Hua Chen Zhi Kang Chen Ming Hua Cong Hu Ma Chun Ling Zhou Wei Wang Qi Luo Yong Mei Shi Yu Mei Qi Hai Ping Jiang Wen Xian Guan Jun Qiang Chen Xiang Hua Wu Jia Xin Chen Yu Fang Lan Zhou Yong Dong Feng Rong Shao Tan Tao Li Jun Wen Ou Qing Chuan Zhao Jian Xiong Wu Min Weng Qing Hua Yao Ya Yin Yu Quan Jun Lyu Hong Qiu Ming Liu Wei Li Han Ping Shi The Investigation on Nutrition Status and Clinical Outcome of Common Cancers(INSCOC)Group | 2022 | Journal of Nutritional Oncology2022，7，2： | 3 |
| 17 | MAGI3 Suppresses Glioma Cell Proliferation via Upregulation of PTEN Expression显示文摘Objective To investigate the role and molecular mechanism of membrane-associated guanylate kinase inverted 3(MAGI3) in glioma cell proliferation. Methods The expression levels of MAGI3 and PTEN were assessed in glioma samples by Western blotting. MAGI3 was stably transfected into C6 glioma cells to obtain C6-MAGI3 cells. Then, the proliferation, the expression levels of MAGI3 and PTEN, and Akt phosphorylation were evaluated in C6 and C6-MAGI3 cells. Xenograft tumor models were established by subcutaneous injection of C6 and C6-MAGI3 cells into nude mice, and the growth rates of xenografts in the mice were compared. The potential role of MAGI3 expression in PI3K/Akt signaling activation was further investigated by examining the correlation between MAGI3 expression and the expression of PI3K/Akt signaling downstream target genes in a glioma dataset using gene set enrichment analysis(GSEA). Results Expression levels of MAGI3 and PTEN were significantly downregulated in gliomas. Overexpression of MAGI3 in the glioma C6 cell line upregulated PTEN protein expression, inhibited the phosphorylation of Akt, and suppressed cell proliferation. MAGI3 overexpression also inhibited the growth of C6 glioma tumor xenografts in nude mice. Analysis based on the GEO database confirmed the negative correlation between activation of PI3K/Akt pathway and MAGI3 mR NA levels in human glioma samples. Conclusion The loss of MAGI3 expression in glioma may enhance the proliferation of glioma cells via downregulation of PTEN expression, leading to the activation of the PI3K/Akt pathway. MAGI3 is a potential glioma suppressor. | MA Qian ZHANG Yan MENG Ran XIE Kun Ming XIONG Ying LIN Song HE Zong Lin K. TAO Tao YANG Ying ZHAO Ji Zong HE Jun Qi | 2015 | Biomedical and Environmental Sciences2015，28，7： | 3 |
| 18 | An inferred functional impact map of genetic variants in rice显示文摘Interpreting the functional impacts of genetic variants(GVs)is an important challenge for functional genomic studies in crops and next-generation breeding.Previous studies in rice(Oryza sativa)have focused mainly on the identification of GVs,whereas systematic functional annotation of GVs has not yet been performed.Here,we present a functional impact map of GVs in rice.We curated haplotype information for 17397026 GVs from sequencing data of 4726 rice accessions.We quantitatively evaluated the effects of missense mutations in coding regions in each haplotype based on the conservation of amino acid residues and obtained the effects of 918848 non-redundant missense GVs.Furthermore,we generated high-quality chromatin accessibility(CA)data from six representative rice tissues and used these data to train deep convolutional neural network models to predict the impacts of 5067405 GVs for CA in regulatory regions.We characterized the functional properties and tissue specificity of the GV effects and found that large-effect GVs in coding and regulatory regions may be subject to selection in different directions.Finally,we demonstrated how the functional impact map could be used to prioritize causal variants in mapping populations.This impact map will be a useful resource for accelerating gene cloning and functional studies in rice,and can be freely queried in RiceVarMap V2.0(http://gffzz90f9113514c343b0honxv59wf0nbu6oxv.ffgz.tsg.suse.edu.cn). | Hu Zhao Jiacheng Li Ling Yang Gang Qin Chunjiao Xia Xingbing Xu Yangmeng Su Yinmeng Liu Luchang Ming Ling-Ung Chen Lizhong Xiong Weibo Xie | 2021 | Molecular Plant2021，14，9： | 3 |
| 19 | Theoretical Studies on the Conformation of mono-6-O-p-nitrobenzoyl-β-cyclodextrin in Aqueous Solution显示文摘The conformation of mono-6-O-p-nitrobenzoyl-β-cyclodextrin was studied with B3LYP/6-311g**//PM3 calculations in vacuum and in water solution. It was concluded that the p-nitrobenzoyl group of the molecule should be located above the primary hydroxyls of the cyclodextrin instead of being completely enclosed by the cavity. It was proposed that the behavior might be caused by the requirement of a cis ester bond in the self-included conformation of the molecule. | Yong FENG, Hai Ming ZHANG, Lei LIU, Yong Hui WANG, Zhao Xiong LIANG, Qing Xiang GUO* Department of Chemistry, University of Science and Technology of China, Hefei 230026 | 2001 | Chinese Chemical Letters2001，12，7： | 2 |
| 20 | Hepatitis B virus-associated follicular lymphoma presents T-cell inflamed phenotype and response to lenalidomide显示文摘Dear Editor,Follicular lymphoma(FL)represents the most common subtype of indolent non-Hodgkin's lymphoma(NHL)with distinct pathological,cytogenetic,and molecular features,accounting for 9.7%of NHLs in China[1].FL maintains a differentiation stage similar to germinal center(GC)B cells and is divided into grades 1,2,3A,and 3B[2].Up to 90%of FLs harbor the t(14;18)(q32;q21)/BCL2 apoptosis regula-tor(BCL2)-immunoglobulin heavy locus(IGH)transloca-tion and epigenetic modifier mutations,particularly lysine methyltransferase 2D(KMT2D)and CREB-binding pro-tein(CREBBP)[2]. | Nan Wang Wei Qin Zhong Zheng Ming Zhao Jie Xiong Hai Fang Rui Sun Yichao Wang Chen Li Lei DongXiaojian Sun Li Wang Pengpeng Xu Shu Cheng Haimin Xu Fan Zhang Weili Zhao | 2022 | Cancer Communications2022，42，2： | 2 |
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