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| 1 | Plankton community composition in the Three Gorges Reservoir Region revealed by PCR-DGGE and its relationships with environmental factors显示文摘To explore the relationships between community composition and the environment in a reservoir ecosystem, plankton communities from the Three Gorges Reservoir Region were studied by PCR-denaturing gradient gel electrophoresis fingerprinting. Bacterial and eukaryotic operational taxonomic units (OTUs), generated by DGGE analysis of the PCR-amplified 16S and 18S rRNA genes, were used as surrogates for the dominant 'biodiversity units'. OTU composition among the sites was heterogeneous; 46.7% of the total bacterial OTUs (45) and 64.1% of the eukaryotic OTUs (39) were identified in less than half of the sampling sites. Unweighted pair group method with arithmetic averages (UPGMA) clustering of the OTUs suggested that the plankton communities in the Xiangxi Rive sites were not always significantly different from those from the Yangtze River sites, despite clear differences in their environmental characterizations. Canonical correspondence analysis (CCA) was applied to further investigate the relationships between OTU composition and the environmental factors. The first two CCA ordination axes suggested that the bacterial community composition was primarily correlated with the variables of NO3--N, dissolved oxygen (DO), and SiO32--Si, whereas, the eukaryotic community was mainly correlated with the concentrations of DO, PO43--P, and SiO32--Si. | YAN Qingyun YU Yuhe FENG Weisong YU Zhigang CHEN Hongtao | 2008 | Journal of Environmental Sciences2008，20，6： | 27 |
| 2 | Impacts of algal blooms removal by chitosan-modified soils on zooplankton community in Taihu Lake,China显示文摘It is important to assess the eflect on zooplankton when perform the environmental protection or restoration technology,especially removing algal blooms,because algae were the major primary producer in algal lakes.The influence on zooplankton community after half a year of algal blooms removed by chitosan-modified soils in Taihu Lake was assessed and the rationality of carrying out the process semiannually was evaluated in the present study.Morphological composition and genetic diversity of zooplankton community were investigated by microscope checkup and polymerase chain reaction-denatured gradient gel electrophoresis(PCR-DGGE) .A total of 44 zooplankton taxa(23 protozoa,17 rotifers,3 copepoda and 1 cladocera) were detected by microscope checkup,and a total of 91 bands(28 bands amplified by primers F1427-GC and R1616,63 bands amplified by primers Fung-GC and NS1) were detected by PCR-DGGE.The results of cluster analysis or detrended correspondence analysis indicated that there was no considerable diflerence in morphological composition of zooplankton and DGGE profiles between experimental and control sites,and DGGE profiles could represent the biologic diversity.The study showed that zooplankton community could recover original condition after half year of algal blooms removed by chitosan-modified soils and it was acceptable to apply this process semiannually.In addition,the results revealed that PCR-DGGE could be applied to investigate the impacts of the environmental protection or restoration engineering on zooplankton community diversity. | Jiajia Ni Yuhe Yu Weisong Feng Qingyun Yan Gang Pan Bo Yang Xiang Zhang Xuemei Li | 2010 | Journal of Environmental Sciences2010，22，10： | 8 |
| 3 | Genetic fingerprinting of plankton community provides new insights into aquatic ecology显示文摘在过去的二十年,分子的技术广泛地在生态的学习被使用了,分子的生态学在上生态学的最重要的分支。同时,基因采指纹分析显著地提高了我们差异的知识和板补品有机体的进化关系。与在生态的学习的常规途径相比(例如词法分类) ,在浮游生物研究的基因采指纹技术。这概述的目的是评定我们在哪儿,我们现在在哪儿并且什么为与分子水平的信息解决水的生态的问题的未来抓住。 | YAN Qingyun YU Yuhe FENG Weisong | 2006 | Progress in Natural Science:Materials International2006，16，9： | 8 |
| 4 | Metagenome-based analysis:A promising direction for plankton ecological studies显示文摘The plankton community plays an especially important role in the functioning of aquatic ecosystems and also in biogeochemical cycles. Since the beginning of marine research expeditions in the 1870s, an enormous number of planktonic organisms have been described and studied. Plankton investigation has become one of the most important areas of aquatic ecological study, as well as a crucial component of aquatic environmental evaluation. Nonetheless, traditional investigations have mainly focused on morphospecies composition, abundances and dynamics, which primarily depend on morphological identification and counting under microscopes. However, for many species/groups, with few readily observable characteristics, morphological identification and counting have historically been a difficult task. Over the past decades, microbiologists have endeavored to apply and extend molecular techniques to address questions in microbial ecology. These culture-independent studies have generated new insights into microbial ecology. One such strategy, metagenome-based analysis, has also proved to be a powerful tool for plankton research. This mini-review presents a brief history of plankton research using morphological and metagenome-based approaches and the potential applications and further directions of metagenomic analyses in plankton ecological studies are discussed. The use of metagenome-based approaches for plankton ecological study in aquatic ecosystems is encouraged. | YAN QingYun YU YuHe | 2011 | Science China(Life Sciences)2011，54，1： | 4 |
| 5 | Comparative study on the gut microbiotas of four economically important Asian carp species显示文摘Gut microbiota of four economically important Asian carp species(silver carp, Hypophthalmichthys molitrix; bighead carp,Hypophthalmichthys nobilis; grass carp, Ctenopharyngodon idella; common carp, Cyprinus carpio) were compared using 16 S rRNA gene pyrosequencing. Analysis of more than 590,000 quality-filtered sequences obtained from the foregut, midgut and hindgut of these four carp species revealed high microbial diversity among the samples. The foregut samples of grass carp exhibited more than 1,600 operational taxonomy units(OTUs) and the highest alpha-diversity index, followed by the silver carp foregut and midgut. Proteobacteria, Firmicutes, Bacteroidetes and Fusobacteria were the predominant phyla regardless of fish species or gut type. Pairwise(weighted) UniFrac distance-based permutational multivariate analysis of variance with fish species as a factor produced significant association(P<0.01). The gut microbiotas of all four carp species harbored saccharolytic or proteolytic microbes, likely in response to the differences in their feeding habits. In addition, extensive variations were also observed even within the same fish species. Our results indicate that the gut microbiotas of Asian carp depend on the exact species, even when the different species were cohabiting in the same environment. This study provides some new insights into developing commercial fish feeds and improving existing aquaculture strategies. | Xinghao Li Yuhe Yu Chang Li Qingyun Yan | 2018 | Science China(Life Sciences)2018，61，6： | 4 |
| 6 | LASER GLAZING OF Ni-Nb AND Ni-Nb-Cr ALLOYS AND CORROSION RESISTANCE OF AMORPHOUS LAYERS显示文摘Partially amorphous Ni-Nb alloy was made by 1.91 kW cw CO2 laser melt-quenching,and fully amorphous by 4.5 kW and 4.9 kW.The corrosion resistance of the amor-phous layer was improved by adding Cr.Potentiodynamic polarization curves showthat the amorphous Ni50Nb40Cr10 is in more corrosion-resistant than the amorphousNi60Nb40and 18-8 stainless steel. | YAN Yuhe YANG Yongqiang ZHAO Changming Tianjin Institute of Textile Science and Technology,Tianjin,ChinaWU Weitao WANG Maocai Institute of Corrosion and Protection of Metals,Academia Sinica,Shenyang,ChinaLU Mengfu Tianjin Institute of Science and Technology,Tianjin,China Lecturer,Laser Processing Lab.,Tianjin Institute of Textile Science and Technology,Tianjin 300160,China | 1993 | Acta Metallurgica Sinica(English Letters)1993，6，7： | 1 |
| 7 | Molecular cloning and identification of full-length cDNA encoding hjgh afinity Fc receptor for bovine IgG(FcγRI)显示文摘 | Yuhe Yan Xuewu Li Aiping Wang | 2000 | Veterinary Immunology and Immunopathology2000，75，： | 1 |
| 8 | Plankton Community Succession in Artificial Systems Subjected to Cyanobacterial Blooms Removal using Chitosan-Modified Soils显示文摘 | Qingyun Yan Yuhe Yu Weisong Feng Gang Pan Hao Chen Juan Chen Bo Yang Xuemei Li Xiang Zhang | 2009 | Microbial Ecology2009，，1： | 1 |
| 9 | Identification of the Secondary Follicle Cycle of Hexi Cashmere Goat显示文摘 | Yan Yuhe Li Xiang-cheng Ji Qing-wang | 2012 | The Anatomical Record2012，295，： | 1 |
| 10 | Molecular cloning and identification of full - length cDNA encoding high afinity Fc receptor for bovine IgG (FcγRI)显示文摘 | Yuhe Yan Xuewu Li Aiping Wang et ol | 2000 | Veterinary Immunology and Immunopathology2000，75，： | 1 |
| 11 | Used lubricating oil recycling using a membrane filtration:Analysis of efficiency,structural and composing显示文摘 | Cao Yuhe Yan Feng Li Jianxin | 2009 | Desalin Water Treat2009，11，13： | 1 |
| 12 | Pionkton Community Succession in Artificial Systems Subjected to Cyanobacterial Blooms Removalusing Chitosan-Modified Soils显示文摘 | Qingyun Yan Yuhe Yu Weisong feng | | 0，，58： | 1 |
| 13 | Novel x-Type High-Molecular-Weight Glutenin Genes From Aegilops tauschii and Their Implications on the Wheat Origin and Evolution Mechanism of Glu-D1-1 Proteins显示文摘 | Zhang Yanzhen Li Xiaohui Wang Aili An Xueli Zhang Qian Pei Yuhe Gao Liyan Ma Wujun Appels Rudi Yan Yueming | 2008 | Genetics2008，，1： | 1 |
| 14 | A New Model for Network Security Situation Assessment of the Industrial Internet显示文摘To address the problem of network security situation assessment in the Industrial Internet,this paper adopts the evidential reasoning(ER)algorithm and belief rule base(BRB)method to establish an assessment model.First,this paper analyzes the influencing factors of the Industrial Internet and selects evaluation indicators that contain not only quantitative data but also qualitative knowledge.Second,the evaluation indicators are fused with expert knowledge and the ER algorithm.According to the fusion results,a network security situation assessment model of the Industrial Internet based on the ER and BRB method is established,and the projection covariance matrix adaptive evolution strategy(P-CMA-ES)is used to optimize the model parameters.This method can not only utilize semiquantitative information effectively but also use more uncertain information and prevent the problem of combinatorial explosion.Moreover,it solves the problem of the uncertainty of expert knowledge and overcomes the problem of low modeling accuracy caused by insufficient data.Finally,a network security situation assessment case of the Industrial Internet is analyzed to verify the effectiveness and superiority of the method.The research results showthat this method has strong applicability to the network security situation assessment of complex Industrial Internet systems.It can accurately reflect the actual network security situation of Industrial Internet systems and provide safe and reliable suggestions for network administrators to take timely countermeasures,thereby improving the risk monitoring and emergency response capabilities of the Industrial Internet. | Ming Cheng Shiming Li Yuhe Wang Guohui Zhou Peng Han Yan Zhao | 2023 | Computers, Materials & Continua2023，，5： | 0 |
| 15 | Saturated absorption spectroscopy of buffergas-cooled Barium monofluoride molecules显示文摘We report an experimental investigation on the Doppler-free saturated absorption spectroscopy of buffer-gas-cooled Barium monofluoride(BaF)molecules in a 4 K cryogenic cell.The obtained spectra with a resolution of 19 MHz,much smaller than previously observed in absorption spectroscopy,clearly resolve the hyperfine transitions.Moreover,we use these high-resolution spectra to fit the hyperfine splittings of excited A(v=0)state and find the hyperfine splitting of the laser-cooling-relevant A^(2)Π_(1/2)(v=0,J=1/2,+)state is about 18 MHz,much higher than the previous theoretically predicted value.This provides important missing information for laser cooling of BaF molecules. | Wenhao Bu Yuhe Zhang Qian Liang Tao Chen Bo Yan | 2022 | Frontiers of physics2022，17，6： | 0 |
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