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| 1 | Reduced function and disassembled microtubules of cultured cardiomyocytes in spaceflight显示文摘Lack of gravity during spaceflight has profound effects on cardiovascular system, but little is known about how the cardiomyocytes respond to microgravity. In the present study, the effects of spaceflight on the structure and function of cultured cardiomyocytes were reported. The primary cultures of neo- natal rat cardiomyocytes were carried on Shenzhou-6 spacecraft and activated at 4 h in orbit. 8 samples were fixed respectively at 4, 48 and 96 h after launching for immunofluorescence of cytoskeleton, and 2 samples remained unfixed to analyze contractile and secretory functions of the cultures. Ground sam- ples were treated in our laboratory in parallel. After 115 h spaceflight, video recordings displayed that the number of spontaneous beating sites in flown samples decreased significantly, and the cells in the beating aggregate contracted in fast frequency without synchrony. Radioimmunoassay of the medium showed that the atrial natriuretic peptide secreted from flown cells reduced by 59.6%. Confocal images demonstrated the time-dependant disassembly of mirotubules versus unchanged distribution and or- ganization of microfilaments. In conclusion, above results indicate reduced function and disorganized cytoskeleton of cardiomyocytes in spaceflight, which might provide some cellular basis for further investigations to probe into the mechanisms underlying space cardiovascular dysfunction. | YANG Fen LI YingHui DING Bai NIE JieLin WANG HongHui ZHANG XiaoYou WANG ChunYan LING ShuKuan NI ChengZhi DAI ZhongQuan TAN YingJun WAN YuMin | 2008 | Chinese Science Bulletin2008，53，8： | 5 |
| 2 | The mechanosensitive lncRNA Neat1 promotes osteoblast function through paraspeckle-dependent Smurf1 mRNA retention显示文摘Mechanical stimulation plays an important role in bone remodeling. Exercise-induced mechanical loading enhances bone strength,whereas mechanical unloading leads to bone loss. Increasing evidence has demonstrated that long noncoding RNAs(lnc RNAs) play key roles in diverse biological, physiological and pathological contexts. However, the roles of lnc RNAs in mechanotransduction and their relationships with bone formation remain unknown. In this study, we screened mechanosensing lnc RNAs in osteoblasts and identified Neat1, the most clearly decreased lnc RNA under simulated microgravity. Of note, not only Neat1 expression but also the specific paraspeckle structure formed by Neat1 was sensitive to different mechanical stimulations, which were closely associated with osteoblast function. Paraspeckles exhibited small punctate aggregates under simulated microgravity and elongated prolate or larger irregular structures under mechanical loading. Neat1 knockout mice displayed disrupted bone formation, impaired bone structure and strength, and reduced bone mass. Neat1 deficiency in osteoblasts reduced the response of osteoblasts to mechanical stimulation. In vivo, Neat1 knockout in mice weakened the bone phenotypes in response to mechanical loading and hindlimb unloading stimulation. Mechanistically, paraspeckles promoted nuclear retention of E3 ubiquitin ligase Smurf1 m RNA and downregulation of their translation, thus inhibiting ubiquitination-mediated degradation of the osteoblast master transcription factor Runx2, a Smurf1 target. Our study revealed that Neat1 plays an essential role in osteoblast function under mechanical stimulation, which provides a paradigm for the function of the lnc RNA-assembled structure in response to mechanical stimulation and offers a therapeutic strategy for long-term spaceflight-or bedrest-induced bone loss and age-related osteoporosis. | Caizhi Liu Xingcheng Gao Yuheng Li Weijia Sun Youjia Xu Yingjun Tan Ruikai Du Guohui Zhong Dingsheng Zhao Zizhong Liu Xiaoyan Jin Yinlong Zhao Yinbo Wang Xinxin Yuan Junjie Pan Guodong Yuan Youyou Li Wenjuan Xing Guanghan Kan Yanqing Wang Qi Li Xuan Han Jianwei Li Shukuan Ling Yingxian Li | 2022 | Bone Research2022，10，2： | 3 |
| 3 | Synthesis and characterization of injectable photocrosslinking poly(ethylene glycol) diacrylate based hydrogels显示文摘 | Tan Guoxing Wang Yingjun Li Jun | 2008 | Polymer Bulletin2008，61，1： | 1 |
| 4 | Influence of water states in hydrogels on the transmissibility and permeability of oxygen in contact lens materials显示文摘 | Yingjun Wang Guoxin Tan Shujiang Zhang | 2008 | Applied surface science2008，255，： | 1 |
| 5 | Synthe-sis and characterization of injectable photocrosslinkingpoly(ethylene glycol)diacrylate based hydrogels显示文摘 | TAN Guoxin WANG Yingjun LI Jun | 2008 | Polymer Bulletin2008，61，1： | 1 |
| 6 | Gravity affects the responsiveness of Runx2 to 1, 25-dihydroxyvitamin D3 (VD3)显示文摘 | Feima Guo Zhongquan Dai Feng Wu Zhaoxia Liu Yingjun Tan Yumin Wan Peng Shang Yinghui Li | 2013 | Acta Astronautica2013，，： | 1 |
| 7 | A future laminted bamboo structure residetial building prototype in China:Life cycle assess- ment of energy use and carbon emission 显示文摘 | Yu Dongwei Tan Hongwei Yan Yingjun | 2011 | Energy and Building''2011，43，10： | 1 |
| 8 | Rainfall forecast errors in different landfall stages of Super Typhoon Lekima (2019)显示文摘The rainfall forecast performance of the Tropical Cyclone(TC)version Model of Global and Regional Assimilation PrEdiction System(GRAPESTCM)of the China Meteorological Administration for landfalling Super Typhoon Lekima(2019)is studied by using the object-oriented verification method of contiguous rain area(CRA).The major error sources and possible reasons for the rainfall forecast uncertainties in different landfall stages(including near landfall and moving further inland)are compared.Results show that different performance and errors of rainfall forecast exist in the different TC stages.In the near landfall stage the asymmetric rainfall distribution is hard to be simulated,which might be related to the too strong forecasted TC intensity and too weak vertical wind shear accompanied.As Lekima moves further inland,the rain pattern and volume errors gradually increase.The Equitable Threat Score of the 24 h forecasted rainfall over 100 mm declines quickly with the time-length over land.The diagnostic analysis shows that there exists an interaction between the TC and the mid-latitude westerlies,but too weak frontogenesis is simulated.The results of this research indicate that for the current numerical model,the forecast ability of persistent heavy rainfall is very limited,especially when the weakened landing TC moves further inland. | Bin HE Zifeng YU Yan TAN Yan SHEN Yingjun CHEN | 2022 | Frontiers of Earth Science2022，16，1： | 1 |
| 9 | A future bamboo-structure residential building prototype in China:Life cycle assessment of energy use and carbon emission显示文摘 | Dongwei Yu Hongwei Tan Yingjun Ruan | 2011 | Energy and Buildings2011，43，2011： | 1 |
| 10 | Using of the surface plasmon resonance cytosensor for real-time and non-invasive monitoring of cellular effects in living C6 cells induced by PMA显示文摘Developing novel instruments and technologies for spatio-temporal and dynamic meas-urements of the intricate cellular effects involving molecular translocation, signal transduction, and molecular interactions inside living cells is essential for the cell and molecular biology science. For the purpose of monitoring and investigating molecular events in living cells at real-time, the surface plasmon resonance based cytosensor (SBCS) for cell cultur-ing and signal monitoring was established, and on the basis of it, the corresponding technology was also established by monitoring and analyzing SPR re-sponses induced in rat C6 glioma cells by phorbol 12-myristate 13-acetate (PMA). The SPR signals induced by PMA in living C6 cells were significantly different from those groups without cells. These re-sponses were strongly dependent on and saturable to the concentrations of PMA, and could be sup-pressed by the specific and potent PKC inhibitors, which indicated that the measured signal could be the reflection of the redistribution of intracellular components near the cell membrane triggered by the activation of PKC. This research provides a quantita-tive and non-invasive technique to study the spa-tio-temporal characteristics of the cellular effects in living cells at real-time. Furthermore, this technology could also be widely used in the basic research as well as applied realms, such as space effects evaluation, environmental safety assessment, bio-logical weapon detection, cellular and molecular re-search, and drug screening. | WANG Chunyan LI Yinghui XIONG Jianghui,TAN Yingjun YU Jianru | 2006 | Chinese Science Bulletin2006，51，8： | 1 |
| 11 | A future bamboo-structure residential building prototype in China: Life cycle assessment of energy use and carbon emission显示文摘 | Dongwei Yu Hongwei Tan Yingjun Ruan | 2011 | Energy & Buildings2011，，10： | 1 |
| 12 | A futurebamboo-structure residential building prototype in China: Lifecycle assessment of energy use and carbon emission显示文摘 | YU Dongwei TAN Hongwei RUAN Yingjun | 2011 | Energyand Buildings2011，43，10： | 1 |
| 13 | A future bamboo-struc- ture residential building prototype in China: life cycle assessment of energy use and carbon emission显示文摘 | YU Dongwei TAN Hongwei RUAN Yingjun | 2011 | Energy and Buildings2011，43，10： | 1 |
| 14 | Electrospinning of Neat Graphene Nanofbers显示文摘Macroscopic assembly of graphene sheets has renovated the preparation of neat carbonaceous fbers with integrating high performance and superior functionalities,beyond the pyrolysis of conventional polymeric precursors.To date,graphene microfbers by the liquid crystalline wet-spinning method have been established.However,how to reliably prepare continuous neat graphene nanofbers remains unknown.Here,we present the electrospinning of neat graphene nanofbers enabled by modulating colossally extensional fow state of graphene oxide liquid crystals.We use polymer with mega molecular weight as transient additives to realize the colossal extensional fow and electrospinning.The neat graphene nanofbers feature high electronic quality and crystallinity and exhibit high electrical conductivity of 2.02×10^(6) S/m that is to be comparable with single crystal graphite whisker.The electrospinning of graphene nanofbers was extended to prepare large-area fabric with high fexibility and superior specifc electrical/thermal conductivities.The electrospinning of graphene nanofbers opens the door to nanofbers of rich two-dimensional sheets and the neat graphene nanofbers may grow to be a new species after conventional carbonaceous nanofbers and whiskers in broad functional applications. | Zhanpo Han Jiaqing Wang Senping Liu Qinghua Zhang Yingjun Liu Yeqiang Tan Shiyu Luo Fan Guo Jingyu Ma Peng Li Xin Ming Chao Gao Zhen Xu | 2022 | Advanced Fiber Materials2022，4，2： | 1 |
| 15 | A future bamboo-structure residential building prototype in China: Life cycle assessment of energy use and carbon emission显示文摘 | Dongwei Yu Hongwei Tan Yingjun Ruan | 2011 | Energy & Buildings2011，，10： | 1 |
| 16 | Macroscopic assembled graphene nanofilms based room temperature ultrafast mid-infrared photodetectors显示文摘Graphene with linear energy dispersion and weak electron-phonon interaction is highly anticipated to harvest hot electrons in a broad wavelength range.However,the limited absorption and serious backscattering of hot-electrons result in inadequate quantum yields,especially in the mid-infrared range.Here,we report a macroscopic assembled graphene(nMAG)nanofilm/silicon heterojunction for ultrafast mid-infrared photodetection.The assembled Schottky diode works in 1.5-4.0μm at room temperature with fast response(20-30 ns,rising time,4 mm2 window)and high detectivity(1.61011 to 1.9109 Jones from 1.5 to 4.0μm)under the pulsed laser,outperforming single-layer-graphene/silicon photodetectors by 2-8 orders.These performances are attributed to the greatly enhanced photo-thermionic effect of electrons in nMAG due to its high light absorption(~40%),long carrier relaxation time(~20 ps),low work function(4.52 eV),and suppressed carrier number fluctuation.The nMAG provides a long-range platform to understand the hot-carrier dynamics in bulk 2D materials,leading to broadband and ultrafast MIR active imaging devices at room temperature. | Li Peng Lixiang Liu Sichao Du Srikrishna Chanakya Bodepudi Lingfei Li Wei Liu Runchen Lai Xiaoxue Cao Wenzhang Fang Yingjun Liu Xinyu Liu Jianhang Lv Muhammad Abid Junxue Liu Shengye Jin Kaifeng Wu Miao-Ling Lin Xin Cong Ping-Heng Tan Haiming Zhu Qihua Xiong Xiaomu Wang Weida Hu Xiangfeng Duan Bin Yu Zhen Xu Yang Xu Chao Gao | 2022 | InfoMat2022，4，6： | 1 |
| 17 | Application of GIS in Oilfield Power System显示文摘This paper discusses the use of GIS in oilfield power system,combined with the basic concept and advantages of GIS,combined with the characteristics of the oilfield power system,put forward the overall design system of the oilfield power system,ensure the stability of the power system operation,give full play to the role of GIS. | Yingjun Hao Bin Tan Xiaoli Zheng Long Zhai Yun Sun Sichao Gao | 2020 | Journal of Electronic Research and Application2020，4，2： | 0 |
| 18 | Wrinkling modes of graphene oxide assembled on curved surfaces显示文摘Assembling two-dimensional(2D)sheets into macroscopic three-dimensional(3D)forms has created a promising material family with rich functionalities.Multiscale wrinkles are intrinsic features of 2D sheets in their 3D assembles.Therefore,the precise wrinkling modulation optimizes the transition of outstanding properties of 2D sheets to expected performances of assembled materials and dominates their fabrication process.The wrinkling evolution of 2D sheets assembling onto flat surfaces has been extensively understood,however,the wrinkling behaviors on the more generally curved surface still remain unclear.Here,we investigate the wrinkling behaviors of graphene oxide sheets assembled onto curved surfaces and reveal the selection rule of wrinkling modes that determined by the curvature mismatch between 2D sheets and target surfaces.We uncover that three wrinkling modes including isotropic cracked land,labyrinth,and anisotropic curtain phases,respectively emerge on flat,spherical,and cylindrical surfaces.A favorable description paradigm is offered to quantitatively measure the complex wrinkling patterns and assess the curvature mismatch constraint underlying the wrinkling mode selection.This research provides a general and quantitative description framework of wrinkling modulation of 2D materials such as high performance graphene fibers,and guides the precise fabrication of particles and functional coatings. | Kaiwen Li Zhanpo Han Lidan Wang Jiaqing Wang Chuanwei Zhang Jiahao Lin Shiyu Luo Li Peng Wenzhang Fang Yingjun Liu Ziliang Wu Yeqiang Tan Chao Gao Zhen Xu | 2023 | Nano Research2023，16，2： | 0 |
| 19 | Biomimetic hydroxyapatite coating on the 3D-printed bioactive porous composite ceramic scaffolds promoted osteogenic differentiation via PI3K/AKT/mTOR signaling pathways and facilitated bone regeneration in vivo显示文摘The architecture and surface modifications have been regarded as effective methods to enhance the bi-ological response of biomaterials in bone tissue engineering.The porous architecture of the implanta-tion was essential conditions for bone regeneration.Meanwhile,the design of biomimetic hydroxyap-atite(HAp)coating on porous scaffolds was demonstrated to strengthen the bioactivity and stimulate osteogenesis.However,bioactive bio-ceramics such asβ-tricalcium phosphate(β-TCP)and calcium sili-cate(CS)with superior apatite-forming ability were reported to present better osteogenic activity than that of HAp.Hence in this study,3D-printed interconnected porous bioactive ceramicsβ-TCP/CS scaf-fold was fabricated and the biomimetic HAp apatite coating were constructed in situ via hydrothermal reaction,and the effects of HAp apatite layer on the fate of mouse bone mesenchymal stem cells(mBM-SCs)and the potential mechanisms were explored.The results indicated that HAp apatite coating en-hanced cell proliferation,alkaline phosphatase(ALP)activity,and osteogenic gene expression.Further-more,PI3K/AKT/mTOR signaling pathway is proved to have an important impact on cellular functions.The present results demonstrated that the key molecules of phosphatidylinositol 3-kinase(PI3K),protein kinase B(AKT)and mammalian target of rapamycin(mTOR)were activated after the biomimetic hydrox-yapatite coating were constructed on the 3D-printed ceramic scaffolds.Besides,the activated influence on the protein expression of Runx2 and BMP2 could be suppressed after the treatment of inhibitor HY-10358.In vivo studies showed that the constructed HAp coating promoted bone formation and strengthen the bone quality.These results suggest that biomimetic HAp coating constructed on the 3D-printed bioac-tive composite scaffolds could strengthen the bioactivity and the obtained biomimetic multi-structured scaffolds might be a potential alternative bone graft for bone regeneration. | Bizhi Tan Naru Zhao Wei Guo Fangli Huang Hao Hu Yan Chen Jungang Li Zemin Ling Zhiyuan Zou Rongcheng Hu Chun Liu Tiansheng Zheng Gang Wang Xiao Liu Yingjun Wang Xuenong Zou | 2023 | Journal of Materials Science & Technology2023，，5： | 0 |
| 20 | Progress on research and mitigation of wind-blown sand risk in Dunhuang Singing Sand Mountain and Crescent Spring Scenic area,China显示文摘The Singing Sand Mountain and Crescent Spring Scenic Spot in Dunhuang,Northwest China is a world-renowned desert attraction that is also an integral component of the Dunhuang UNESCO Global Geopark.This scenic area underwent a 30-year transformation,i.e.,from a severe sand risk with spring water threatened by sand burial due to dune deformation,to restoration of the original sand flow field and mitigation of the sand burial problem.The current paper summarizes the research on the intensive monitoring of the dynamic change of star dunes near the spring,observation of wind and sand flow movement,and then restoring the harmonic vibration of the sand particles(singing sand)that were previously silenced.The existing and prospective impacts of anthropogenic and natural forces on the deformation of the sand dunes are investigated by integrated methods,guiding the implementation of mitigating measures with significant ameliorative effects.Contrast to common sand control practices that aim to reduce wind speed and stop blown sands,our research highlights the importance of maintaining the natural wind flow field in stabilizing surrounding dunes.These mitigation measures consist of removing excessive vegetation and newly constructed buildings to recover the original wind flow field and sand transport activity.Such research and mitigation efforts ensure the scientific protection and restoration of the special desert landform,and contribute to the mutual enhancement of the conservation and exploitation of this desert scenic spot and similar sites. | BenLi Liu KeCun Zhang JianJun Qu HaiJiang Li QingHe Niu ZhiShan An YingJun Pang LiHai Tan GenSheng Yang | 2023 | Research in Cold and Arid Regions2023，15，3： | 0 |
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