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| 1 | Tetrapioid induction of Crassostrea hongkongensis and C.sikamea by inhibiting the polar body 1 release in diploid fertilized eggs显示文摘The production of an all-triploid population by mating tetrapioid males with diploid females is the best and most fundamental method for the large-scale production of triploid oysters.Obtaining a stable tetrapioid population is essential for guaranteed production in industrialized triploid cultivation.C.hongkongensis and C.sikamea are important oyster breeding species in southern China,and have great economic value.However,there are not any published data on inducing tetrapioid C.hongkongensis or C.sikamea.Therefore,we investigated tetrapioid induction in these two oyster species by inhibiting the PB1 release in diploid fertilized eggs using Cytochalasin B(CB)under 31℃,15%o salinity.The results confirmed that the optimal tetrapioid induction conditions for C.hongkongensis were a CB concentration of 0.50 mg/L with induction starting at 9.0 min after fertilization,and stopping at 21.0 min after fertilization;the induction efficiency index reached 0.123 under these conditions.The optimal tetrapioid induction conditions for C.sikamea were a CB concentration of 0.50 mg/L,with induction starting at 7.5 min after fertilization and stopping at 18 min after fertilization;the induction efficiency index could be as high as 0.281 under these conditions.However,we confirmed that the tetrapioid rate decreased with larval growth,and no tetrapioids were detected in the juvenile period of either C.hongkongensis or C.sikamea.This may be attributed to the very low survival of the tetrapioid larvae induced by this method,especially as most tetrapioid larvae died during the first three days.In summary,it is simple to directly induce tetrapioid C.hongkongensis and C.sikamea larvae by inhibiting the PB1 release of diploid zygotes,but the low survival rate makes it challenging to obtain viable juvenile tetrapioids. | Yanping Qin Zohaib Noor Xingyou Li Yuehuan Zhang Ziniu Yu Haitao Ma Jun Li Yinyin Zhou Riguan Mo | 2021 | Marine Life Science & Technology2021，3，4： | 1 |
| 2 | Effects of Cognitive Behavioral Therapy on Insomnia of Maintenance Hemodialysis Patients显示文摘 | Yongmei Hou Peicheng Hu Yanping Liang Zhanyu Mo | 2014 | Cell Biochemistry and Biophysics2014，，： | 1 |
| 3 | Application of Boron-doped Diamond Film Electrode in Fast Detection of Samonella in Water显示文摘[Objective]The study aimed to discuss the application of boron-doped diamond( BDD) film electrode in fast detection of samonella in water. [Method]Boron-doped diamond film electrode was prepared and used as the working electrode in fast detection of salmonella in water using chronoamperometry,and the oxidation mechanism of the electrode acting on salmonella was discussed. [Result]Compared with traditional biological methods,chronoamperometry could detect the number of salmonellae in water more simply,rapidly and sensitively. [Conclusion]The method of using BDD electrode to detect salmonella quantity will be widely applied in future. | Baoying Lv Zhaoyu Mo Yanping Tang Yanxian Nong | 2013 | Meteorological and Environmental Research2013，4，10： | 0 |
| 4 | Inducible Expression on Multi-epitope of Porcine Circovirus Type 2 and Its Immunological Competence显示文摘In order to obtain induction expressed porcine circovirus type 2(PCV2)multi-epitope with good immunogenicity in vitro.Major epitopes of PCV2 were screened in the test,epitopes were composed sequentially,the c DNA sequences were artificially synthesized.Bam HⅠand SalⅠwere directionally cloned into prokaryotic expression vector PEGX-4T-1 multiple cloning site,then BL21 competent cells were transformed,positive clones were screened,IPTG inducible expression was conducted.Expression on target gene was analyzed by SDS-PAGE,fusion protein polypeptide was extracted and purified,immunocompetence of the expressed multi-epitope protein was identified by Westernblot.BALB/c mouse was immuned by fusion protein polypeptide,the antibody was determined by ELISA,immunogenicity was evaluated.Results showed that expression recombinant plasmid pEGX-4T-1-ep contained with seven PCV2 antigen epitopes had been constructed successfully.SDS-PAGE analysis showed that fusion protein polypeptide was expressed effectively in Escherichia coli(E.coli),and the molecular weight was about 35ku,which existed in the form of solubility.Results of Westernblot showed that the extraction and purification of fusion protein polypeptide and PCV2 positive serum had good reactogenicity.Results of ELISA showed that the purified fusion protein polypeptide could stimulate the body to produce PCV2 specific antibody which had good immunogenicity.Results indicated that the constructed PCV2 multi-epitope had good expression characteristics in vitro,and the expression protein had good immunogenicity.The study provided a basic for the study on PCV2 epitope screening,functional identification and multi-epitope vaccine. | Dong Lin Wang Yanping Wang Jinliang Mo Ling Shen Zhiqiang Liu Zengshan | 2016 | Animal Husbandry and Feed Science2016，8，3： | 0 |
| 5 | Nitrogen‐doped tin oxide electron transport layer for stable perovskite solar cells with efficiency over 23%显示文摘Tin oxide has made a major breakthrough in high-efficiency perovskite solar cells(PSCs)as an efficient electron transport layer by the low-temperature chemical bath deposition method.However,tin oxide often contains pernicious defects,resulting in unsatisfactory performance.Herein,we develop high-quality tin oxide films via a nitrogen-doping strategy for high-efficiency and stable planar PSCs.The aligned energy level at the interface of doped SnO_(2)/perovskite,more excellent charge extraction and reduced nonradiative recombination contribute to the enhanced efficiency and stability.Correspondingly,the power conversion efficiency of the devices based on N‐SnO_(2) film increases to 23.41% from 20.55% of the devices based on the pristine SnO_(2).The N-SnO_(2) devices show an outstanding stability retaining 97.8% of the initial efficiency after steady-state output at a maximum power point for 600s under standard AM1.5G continuous illumination without encapsulation,while less than 50% efficiency remains for the devices based on pristine SnO_(2).This simple scalable strategy has shown great promise toward highly efficient and stable PSCs. | Yanping Mo Chao Wang Xuntian Zheng Peng Zhou Jing Li Xinxin Yu Kaizhong Yang Xinyu Deng Hyesung Park Fuzhi Huang Yi‐Bing Cheng | 2022 | Interdisciplinary Materials2022，1，2： | 0 |
| 6 | “Coffee ring” controlment in spray prepared >19% efficiency Cs_(0.19)FA_(0.81)PbI_(2.5)Br_(0.5) perovskite solar cells显示文摘Achieving high-quality perovskite films with uniform morphology and homogeneous crystallinity is challenging owing to the coffee ring effect(CRE) in the spray-coating technologies. In this study, an evaporation/spray-coating two-step deposition method is used to fabricate Cs_(0.19)FA_(0.81)PbI_(2.5)Br_(0.5)light harvesters for perovskite solar cells(PSCs). Considering the solid–liquid reaction, we establish a reaction-dependent regulating strategy that inhibits CRE successfully and prepare a high-quality perovskite layer, wherein the solvent for the FAI/Br solution during the spraying process is changed from isopropanol to n-butyl alcohol(NBA). The retarded-drying-enhanced spreading of the NBA solution inhibits contact line pinning to suppress the capillary flows and increases the reaction between metal halides(CsI/PbI_(2)) and organic salts(FAI/Br), which result in a reduction in the accumulation of solutes in the periphery effectively inhibiting CRE. Consequently, we obtain a high performance Cs_(0.19)FA_(0.81)PbI_(2.5)Br_(0.5) PSC with a power conversion efficiency(PCE) of 19.17%. An enlarged perovskite film(10 × 10 cm^(2)) containing 40 sub-cells is prepared. The average PCE of these devices is 18.33 ± 0.56%, proving the reliability of the 'coffee ring' regulating strategy. This study provides an effective approach for CRE controlment in spraying technology to achieve high repeatability devices with good performance. | Xinxin Yu Jing Li Yanping Mo Tianxing Xiang Zhiliang Ku Fuzhi Huang Fei Long Yong Peng Yi-Bing Cheng | 2022 | Journal of Energy Chemistry2022，31，4： | 0 |
| 7 | COVID-19 mortality in ICUs associated with critical care staffing显示文摘To the Editor Coronavirus disease 2019(COVID-19)has been around for over a year since December 2019,and the global outlook is not optimistic.Because of its high transmissibility and high mortality,management of the COVID-19 pandemic has become a major challenge for health systems globally,especially for critical care. | Jingjing Xi Lin Zeng Shusheng Li Yuhang Ai Xiandi He Yan Kang Yimin Li Yanping Mo Yue Peng Kejian Qian Bingyu Qin Chunting Wang Jing Yan Fachun Zhou Hua Zhang Penglin Ma | 2021 | Burns & Trauma2021，9，1： | 0 |
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