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| 1 | Genetic variation of Laminaria japonica (Phaeophyta) populations in China as revealed by RAPD markers显示文摘For the population genetics analysis of the naturally grown brown seaweed Laminaria japonica (Laminariales,Phaeophyta) sampled from Dalian,Yantai,Weihai,Rongcheng and Qingdao in China,ten primers were employed to produce 88 bands as revealed by randomly amplified polymorphic DNA (RAPD) markers,and all these bands were polymorphic.According to these band patterns,there were 94 distinct phenotypes occurred in 100 samples indicating the high heterozygosity of this kelp.Dalian population samples showed the highest percentage of polymorphism (71.67%),and also the higher diversity estimated on the basis of the Shannon’s index (8.498),suggesting that this population could be chosen as the best resource for genetic breeding.The highest diversity of Yantai population possibly resulted from the introduction of L.longissima used for interspecific cross breeding with L.japonica cultivated in China.From Dalian southwards to Qingdao,the genetic variation of the five populations became less with a decrease in latitude,possibly due to the natural selection especially of high temperature.The genetic distance (Φ ST values) of the five populations was a little significantly correlated with the geographical distance (r=0.496) at P =0.05 by Mantel’s test.Weihai,Rongcheng and Yantai populations were closely grouped genetically together by Neighbor-joining cluster analysis probably in that the dispersal of the kelp by propagules more easily occurring in the range of relatively short distance.The analysis of molecular variance (AMOVA) also demonstrated that the relatively higher variation occurred among populations (71.49%) at an extremely significant level (P <0.000 1).All these evidence showed that there was a relatively distinct genetic differentiation among the sampled kelp populations,and L.japonica grown in China was also rather heterozygous in heredity. | BI Yanhui HU Yuanjie ZHOU Zhigang | 2011 | Acta Oceanologica Sinica2011，30，2： | 7 |
| 2 | Full-length mRNA sequencing in Saccharina japonica and identification of carbonic anhydrase genes显示文摘The carbonic anhydrases(CAs)are a group of enzymes that play an important role in the absorption and transportation of CO_(2) in Saccharina japonica.They are encoded by a superfamily of genes with seven subtypes that are unrelated in sequence but share conserved function in catalyzing the reversible conversion of CO_(2) and HCO_(3)^(-).Here we have characterized the CA members in the transcriptome of S.japonica using Single-molecule real-time(SMRT)sequencing technology.Approximately 9830.4 megabases from 5,028,003 quality subreads were generated,and they were assembled into 326,512 full-length non-chimeric(FLNC)reads,with an average flnc read length of 2181 bp.After removing redundant sequences,79,010 unique transcripts were obtained of which 38,039 transcripts were successfully annotated.From the full-length transcriptome,we have identified 7 full-length cDNA sequences for CA genes(4α-CAs,1β-CAs and 2γ-CAs)and assessed for their potential functions based on phylogenetic analysis.Characterizations of CAs will provide the ground for future studies to determine the involvement of CAs in inorganic carbon absorption and transportation in S.japonica. | Yanhui Bi Jiali Li Zhigang Zhou | 2019 | Aquaculture and Fisheries2019，4，2： | 4 |
| 3 | Achieving a sub-10 nm nanopore array in silicon by metal-assisted chemical etching and machine learning显示文摘Solid-state nanopores with controllable pore size and morphology have huge application potential.However,it has been very challenging to process sub-10 nm silicon nanopore arrays with high efficiency and high quality at low cost.In this study,a method combining metal-assisted chemical etching and machine learning is proposed to fabricate sub-10 nm nanopore arrays on silicon wafers with various dopant types and concentrations.Through a SVM algorithm,the relationship between the nanopore structures and the fabrication conditions,including the etching solution,etching time,dopant type,and concentration,was modeled and experimentally verified.Based on this,a processing parameter window for generating regular nanopore arrays on silicon wafers with variable doping types and concentrations was obtained.The proposed machine-learning-assisted etching method will provide a feasible and economical way to process high-quality silicon nanopores,nanostructures,and devices. | Yun Chen Yanhui Chen Junyu Long Dachuang Shi Xin Chen Maoxiang Hou Jian Gao Huilong Liu Yunbo He Bi Fan Ching-Ping Wong Ni Zhao | 2021 | International Journal of Extreme Manufacturing2021，3，3： | 2 |
| 4 | Carbon-nanotube-modified glassy carbon electrode for simultaneous determination of dopamine, ascorbic acid and uric acid: The effect of functional groups显示文摘 | Huaqing Bi Yanhui Li Shufeng Liu Peizhi Guo Zhongbin Wei Chunxiao Lv Jizhen Zhang X.S. Zhao | 2012 | Sensors & Actuators: B Chemical2012，，： | 1 |
| 5 | An ionic liquid-modified graphene based molecular imprinting electrochemical sensor for sensitive detection of bovine hemoglobin显示文摘 | Zonghua Wang Feng Li Jianfei Xia Lin Xia Feifei Zhang Sai Bi Guoyu Shi Yanzhi Xia Jingquan Liu Yanhui Li Linhua Xia | 2014 | Biosensors and Bioelectronics2014，，： | 1 |
| 6 | Investigating formate tolerance mechanisms in Saccharomyces cerevisiae and its application显示文摘Current global energy and environmental crisis have spurred efforts towards developing sustainable biotechnological solutions,such as utilizing CO_(2) and its derivatives as raw materials.Formate is an attractive onecarbon source due to its high solubility and low reduction potential.However,the regulatory mechanism of formate metabolism in yeast remains largely unexplored.This study employed adaptive laboratory evolution(ALE)to improve formate tolerance in Saccharomyces cerevisiae and characterized the underlying molecular mechanisms.The evolved strain was applied to produce free fatty acids(FFAs)under high concentration of formate with glucose addition.The results showed that the evolved strain achieved a FFAs titer of 250 mg/L.Overall,this study sheds light on the regulatory mechanism of formate tolerance and provides a platform for future studies under high concentrations of formate. | Kai Wang Yining Liu Zhuoheng Wu Yilu Wu Haoran Bi Yanhui Liu Meng Wang Biqiang Chen Jens Nielsen Zihe Liu Tianwei Tan | 2023 | Green Carbon2023，1，1： | 1 |
| 7 | Complete sequence of chloroplast genome from Sargassum vachellianum(Sargassaceae,Phaeophyceae):Genome structure and comparative analysis显示文摘Sargassum vachellianum is an ecologically important brown alga.It is China-specific and mainly inhabits in rocky intertidal zones in southeast coastal waters of China.In this study,we sequenced its circular complete chloroplast genome(cpDNA)and compared it with cpDNAs from S.vachellianum,S.horneri and S.thunbergii.The complete S.vachellianum cpDNA was 124,582 bp in length and consisted of a pair of inverted repeats(IRs)of 5435 bp,a large single copy(LSC)region of 73,721 bp and a small single copy(SSC)region of 39,991 bp.Totally 160 genes were predicted,including 132 protein-coding genes,four ribosomal RNA genes and 24 tRNA genes,and the coding sequences contributed 77.48%of the whole genome.In addition,25 SSR loci and 28 highly variable regions were identified from the S.vachellianum cpDNA,which might be used as candidates for developing DNA barcode markers of Sargassum species.The phylogenetic tree based on datasets of all the plastid-encoded proteins demonstrated that species of S.subgenus Bactrophycus were firstly combined and then clustered with S.vachellianum,which belongs to S.subgen.Sargassum.The results indicate that the chloroplast genomes are good resources for developing new DNA markers for taxonomy,and also as tools for evolutionary research of closely related species in future studies. | Yanhui Bi Jiali Li Zhigang Zhou | 2017 | Aquaculture and Fisheries2017，2，4： | 0 |
| 8 | Transcriptome analysis of gill from Lateolabrax maculatus and aqp3 gene expression显示文摘The Lateolabrax maculatus is an important marine fish in China that can adapt to a wide range of water salinities.The gill is the main organ involved in fish osmoregulation and in this study,a cDNA library of the L.maculatus gill was constructed to understand gill function in Lateolabrax maculatus and it adaptation to salinity.A total raw data of 5.3G base pairs was obtained,followed removal of low-quality and linker DNA sequences and it yielded a total of 23,038,590 clean reads.After splicing and clustering,31,976 unigenes were obtained.The average transcript length was 1327 bp and N50 was 2057 bp.In comparisons with other tissues analysis of the gill transcriptome revealed a high level of relative expression of apolipoprotein E,CD74,keratin,type I cytoskeleton 13,carbonic anhydrase,betaine-homocysteine S-methyltransferase,and aquaporin 3(AQP3)genes.The L.maculatus aqp3(Lm-aqp3)gene was specific for the gills Lm-aqp3 and this gene has a typical transmembrane structure of six alpha helices and a water channel domain.Phylogenetic analysis indicated that Lm-aqp3 clusters with the homologue genes from Labrus bergylta and Dicentrarchus labrax.Quantitative RT-PCR confirmed that Lm-aqp3 was highly abundant in the gills and low expressed in other tissues(stomach,liver,brain,spleen and testis).Analysis of Lm-aqp3 in freshwater aquaculture fish revealed that gene expression was significantly statistical higher than in seawater(20 ppt)fish,suggesting that Lm-aqp3 may play an important role in water metabolism and osmotic pressure regulation in L.maculatus. | Yawei Shen Yudong He Yanhui Bi Jianpeng Chen Ziwei Zhao Jun Li Xiaowu Chen | 2019 | Aquaculture and Fisheries2019，4，6： | 0 |
| 9 | Molecular and FISH analysis of 45S rDNA on BAC molecule of Saccharina japonica显示文摘IGS is abundant in polymorphism,which is widely used in the analysis of intraspecific genetic diversity and phylogenetic relationships among geographical populations.In this study,the 45S rDNA repeat unit of Saccharina japonica was obtained for the first time by BAC clone sequencing.The total length of the 45S rDNA repeat unit of S.japonica was 8995 bp,including 5420 bp of 18S-5.8S-25S rDNA,and 3575 bp of IGS(Intergenic Spacer),with the GC content of 51.4%.The IGS was composed of a 465 bp of 3′-outer transcribed spacer(ETS),an 874 bp 5′-ETS,and a 2236 bp non-transcribed spacer(NTS),with the GC content of 50.1%.Fiber-FISH(fiber-fluorescence in situ hybridization)analysis of the distribution of 45S rDNA repeat units on the bacterial artificial chromosome illustrated that each fiber had at least five continuously moniliform hybridization signal points.This study provided a new candidate molecular marker for detecting intraspecific polymorphisms of S.japonica.In addition,the successful fiber-FISH analysis of the 45S rDNA on BAC molecule would contribute to the construction of the physical map and map-based cloning of this kelp. | Pengfei Liu Yanhui Bi Qian Zheng Li Liu Yu Du Yu Liu Zhigang Zhou | 2023 | Aquaculture and Fisheries2023，8，2： | 0 |
| 10 | Nonabelian omni-Lie algebroids显示文摘In this paper,we study the structure of nonabelian omni-Lie algebroids.Firstly,taking Lie algebroid(E,[·,·]_(E,ρE))as the starting point,a nonabelian omni-Lie algebroid is defined on direct sum bundle DE⊕JE,where DE and JE are,respectively,the gauge Lie algebroid and the jet bundle of vector bundle E,and study its properties.Furthermore,it is concluded that the nonabelian omni-Lie algebroid is a trivial deformation of the omni-Lie algebroid,and the nonabelian omni-Lie algebroid is a matched pair of Leibniz algebroids. | Yanhui BI Hongtao FAN Danlu CHEN | 2022 | Frontiers of Mathematics in China2022，17，6： | 0 |
| 11 | Chromosomal mapping of 5S and 18S-5.8S-25S rRNA genes in Saccharina japonica(Phaeophyceae)as visualized by dual-color fluorescence in situ hybridization显示文摘It has been reported that there was a linkage of 5S rRNA gene to 18S-5.8S-25S rRNA gene in a few of species in Ochrophyta.In regard to the usual two positions of linked 5S rDNA to the 3′end of 25S rDNA,two pairs of primers were designed for amplification to verify this linkage of two genes in a kelp cultivar of Saccharina japonica,one of species in Ochrophyta.This result supplemented the previous report that 5S rDNA was unlinked to 25S rDNA in this kelp.In order to simultaneously visualize this unlinkage of two genes,dual-color fluorescence in situ hybridization(FISH)technique was applied to the cytogenetics of S.japonica.Dual-color FISH images showed that two and four hybridization signals were present in the kelp gametophyte and sporophyte,respectively,metaphase nuclei hybridized simultaneously with the labeled probes of 18S rDNA and 5S rDNA.Both haploid and diploid karyotypes in decreasing length of chromosomes showed that 18S-5.8S-25S rDNA was localized at the interstitial region of Chromosome 23,whereas 5S rDNA resided at the sub-telomeric region of Chromosome 27.These karyotypes suggested that the kelp nuclear genome had only one locus of each rRNA gene,and their loci on different chromosomes indicated the physical unlinkage of 5S rDNA to 18S-5.8S-25S rDNA in this kelp.Therefore,dual-color FISH seems to be a powerful technique for the discrimination and pairing of chromosomes featured in both small size and nearly identical shape in S.japonica. | Yu LIU Pengfei LIU Yanhui BI Zhigang ZHOU | 2021 | Journal of Oceanology and Limnology2021，39，2： | 0 |
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