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| 1 | First light of the 1.8-m solar telescope–CLST显示文摘Large-aperture solar telescopes play an important role in solar observations and research,and require high temporal and spatial resolution[1].To solve some fundamental problems such as the solar dynamo,coronal heating,and the triggering of major solar eruptions,the spatial resolution for solar-atmosphere observation should reach at least 0.1 arcsec[2]. | ChangHui Rao NaiTing Gu XueJun Rao Cheng Li LanQiang Zhang JinLong Huang Lin Kong Ming Zhang YunTao Cheng Yi Pu Hua Bao YouMing Guo YangYi Liu JinSheng Yang LiBo Zhong ChangJun Wang Kai Fang XiaoJun Zhang DongHong Chen Cheng Wang XinLong Fan ZhiWu Yan KeLe Chen XiYa Wei Lei Zhu Hong Liu YongJian Wan Hao Xian WenLi Ma | 2020 | Science China(Physics,Mechanics & Astronomy)2020，63，10： | 6 |
| 2 | A high over-potential binder-free electrode constructed of Prussian blue and MnO2 for high performance aqueous supercapacitors显示文摘Extending the potential window of aqueous supercapacitors (SCs) up to 2.0 V is still a great challenge.Based on their good dynamic structural reversibility and open framework structure,the coordination superamolecular networks (CSNs) exhibit rapid charge/discharge ability and excellent cycle stability.As a typical coordination superamolecular network (CSN),Prussian blue (denoted as CSN-PB),which self-assembled by the CN-ligand and iron ions is firstly in-situ grown on carbon cloth,followed by electro-deposition of MnO2 to form CSN-PB/MnO2 composite electrode.Benefiting from synergistic effect of the constituent components,as well as the open framework structure of CSN-PB,this composite electrode reaches a high potential window of 1.4 V (vs.Ag/AgCl) and delivers a good specific capacitance of 315.3 F·g^-1 in aqueous electrolyte.An aqueous asymmetric device,constructed with CSN-PB/MnO2 composite as cathode and activated carbon as anode,can work in a stable potential window of 2.4 V,exhibits a high energy density of 46.13 Wh·kg^-1 and excellent cycling stability with 85.5% capacitance retention after 20,000 cycles.This work provides a new concept of high dynamic structural reversibility from CSNs to increase the cell voltage of asymmetric SCs for further boosting energy density. | Gaowei Zhang Hua Yao Feng Zhang Zitao Gao Qiujun Li Yangyi Yang Xihong Lu | 2019 | Nano Research2019，12，5： | 5 |
| 3 | A local homogenization approach for simulating the reinforcement effect of the fully grouted bolt in deep underground openings显示文摘Fully grouted bolts are a key component of the support system for underground openings.Although considerable effort has been made in the simulation of the reinforcement effect of the fully grouted bolts on the rock masses surrounding underground openings,most of the work has limited significance since the structural element approach is used.This study proposes a local homogenization approach(L-H approach)that integrates elastoplastic mechanics,composite mechanics,and analytical approaches with numerical simulation to effectively simulate the reinforcement effect of the fully grouted bolt on deep surrounding rock masses.In the L-H approach,the representative volume of bolted rock mass(RVBRM)with a fully grouted bolt is established based on the original mesh model utilized in the rockbolt element approach.The RVBRM is a regular quadrangular prism with a cross-sectional size equal to the bolt spacing and a length equal to the bolt length.The RVBRM is homogenized by the L-H approach from a unidirectional bolt-reinforced composite into a homogeneous transversely isotropic medium whose mechanical properties are described by a new transversely isotropic elastoplastic model.The L-H parameters for the RVBRM are obtained using analytical approaches,composite mechanics,and known parameters of the rock mass and bolt.Using the L-H approach,the reinforcement effect of the fully grouted bolt on the bolted rock specimen and the surrounding rock mass in Jinping II Diversion Tunnel#2 with a depth greater than 2000 m is simulated.The results show that the predictions of the L-H approach are more in agreement with the physical model results of bolted rock specimen and provide a more realistic response of the bolted surrounding rock mass.The L-H approach demonstrates that fully grouted bolts with common bolt spacings and diameters substantially enhance the elastic modulus,shear strength,and tensile strength of the rock mass in the direction of the bolt axis. | Dingping Xu Xiuyang Liu Quan Jiang Shaojun Li Yangyi Zhou Shili Qiu Fei Yan Hong Zheng Xiang Huang | 2022 | International Journal of Mining Science and Technology2022，32，2： | 3 |
| 4 | Effect of Lamb Age and in vitro Culture System of Oocytes on JIVET Technology显示文摘[Objectives] This study was conducted to investigate the effects of lamb age and in vitro culture system of oocytes on the results of juvenile in vitro embryo transfer( JIVET). [Methods]Ten Dorper × small-tailed Han lambs aged 5 to 10 weeks were induced to superovulate via i. p. injection of pregnant mare's serum gonadotropin( PMSG). The oocytes were matured in basal maturation solution or modified maturation solution,which was prepared by adding 200 μmol/L cysteine to the basal maturation solution. Then,the oocytes were fertilized in fertilization medium I containing 2% estrus sheep serum( ESS) or fertilization medium II containing 3 mg/ml bull serum albumin( BSA). Finally,the number of oocytes,oocyte maturation rate and cleavage rate of the lambs of different ages were determined. [Results]The average number of oocytes recovered per lamb was( 111. 00 ± 16. 97),( 139. 50 ± 28. 99),( 108. 50 ± 17. 68) and( 42. 00 ± 11. 31) for5-,7-,8-and 10-week-old Dorper × small-tailed Han lambs,respectively. The number of oocytes obtained from 5-,7-and 8-week-old lambs was significantly higher than that from 10-week-old lambs( P < 0. 05),but there was no significant difference among 5-,7-and 8-week-old lambs( P > 0. 05). The maturation rate of oocytes cultured in modified maturation solution was 3. 64% higher than that in basal maturation solution. The cleavage rate of oocytes in fertilization medium I was very significantly higher than that in fertilization medium II( P < 0. 01). [Conclusions] The results of JIVET can be improved by harvesting oocytes from lambs aged 5-8 weeks,adding a certain amount of cysteine into oocyte maturation solution,and a certain amount of ESS into fertilization medium. | Yangyi MAO Huidi LUO Guanwu ZHANG Zhiwu WANG Peng ZHAO Jun LI Hongyu GUO Shenghua ZHOU Huihui GUO Chunyan LI | 2019 | Agricultural Biotechnology2019，8，1： | 3 |
| 5 | EAST-Educational Adaptive-optics Solar Telescope显示文摘For the public having a better understanding of solar activities,the Educational Adaptive-optics Solar Telescope(EAST)was built in July 2021 and is located at the Shanghai Astronomy Museum.The EAST consists of a 65 cm aperture solar telescope with a 177-element adaptive optics system and two-channel high resolution imaging system at the Hαand TiO bands,in addition to three full disk solar telescopes at CaK,Hαand TiO bands equipped on the tube of the main telescope.In this paper,the configuration of the EAST is described.Its performance and on-sky observational results are presented.The EAST,to our knowledge,is the most advanced solar telescope for the popularization of science in the world.Due to its excellent performance,the data acquired by the EAST can also be used for research on solar physics and space weather prediction. | Changhui Rao Xuejun Rao Zhimao Du Hua Bao Cheng Li Jinlong Huang Youming Guo Libo Zhong Qing Lin Xin Ge Jinsheng Yang Xinlong Fan Yangyi Liu Dan Jia Xin Li Mei Li Ming Zhang Yuntao Cheng Jiahui Zhou Jiawen Yao Lanqiang Zhang Naiting Gu | 2022 | Research in Astronomy and Astrophysics2022，22，6： | 3 |
| 6 | Highly N/O co-doped ultramicroporous carbons derived from nonporous metal-organic framework for high performance supercapacitors显示文摘A new nonporous Zn-based metal-organic framework(NPMOF) synthesized from a high nitrogencontaining rigid ligand was converted into porous carbon materials by direct carbonization without adding additional carbon sources.A series of NPMOF-derived porous carbons with very high N/O contents(24.1% for NPMOF-700,20.2% for NPMOF-800,15.1% for NPMOF-900) were prepared by adjusting the pyrolysis temperatures.The NPMOF-800 fabrica ted electrode exhibits a high capacitance of220 F/g and extremely large surface area normalized capacitance of 57.7 μF/cm~2 compared to other reported MOF-derived porous carbon electrodes,which could be attributed to the abundant ultramicroporosity and high N/O co-doping.More importantly,symmetric supercapacitor assembled with the MOF-derived carbon manifests prominent stability,i.e.,99.1 % capacitance retention after 10,000 cycles at 1.0 A/g.This simple preparation of MOF-derived porous carbon materials not only finds an application direction for a variety of porous or even nonporous MOFs,but also opens a way for the production of porous carbon materials for superior energy storage. | Yangyi Gu Ling Miao Ying Yin Mingxian Liu Lihua Gan Liangchun Li | 2021 | Chinese Chemical Letters2021，32，4： | 2 |
| 7 | JIVET Research on Texel Sheep显示文摘The present study was conducted to explore ways of JIVET research on Texel sheep,and to provide technical support for the improvement of the JIVET technology system for research on Texel sheep.Superovulation was conducted on 4-8-week old Texel sheep,and in-vivo ovum collection,in-vitro oocyte maturation and fertilization,and fertilized-embryo culture and transplantation were adopted to perform JIVET research on Texel sheep.The results showed that 296 available oocytes were obtained at an average of 74 oocytes per sheep;205 fertilized eggs were obtained at the 2-4 cell stage at an average of 51.25 eggs per sheep and a cleavage rate of 69.26%;and 66 of the fertilized eggs were transplanted to 11 receptor sheep.Six sheep were conceived at a conception rate of 54.55%,and 11 lambs were born.These results indicated that the present test could be used as a method for the study of JIVET technique on Texel sheep. | Yangyi MAO Huidi LUO Xi LI Peng ZHAO Jun LI Sujun WU Hongyu GUO | 2020 | Agricultural Biotechnology2020，9，5： | 2 |
| 8 | Regulation of the order-disorder phase transition in a Cs_(2)NaFeCl_(6) double perovskite towards reversible thermochromic application显示文摘Multifunctional lead-free double perovskites demonstrate remarkable potential towards applications in various fields.Herein,an environmentally-friendly,low-cost,high-throughput Cs_(2)NaFeCl_(6) single crystal with exceedingly high thermal stability is designed and grown.It obtains a cubic lattice system in the temperature range of 80-500 K,accompanied by a completely reversible chromatic variation ranging from yellow to black.Importantly,the intriguing thermochromism is proved to own extremely high reproducibility(over 1000 cycles)without a hysteretic effect,originating from its structural flexibility that including(i)the noteworthy distortion/deformation of[NaCl_(6)]5−and[FeCl_(6)]3−octahedra;(ii)order-disorder arrangement transition of[NaCl_(6)]5−and[FeCl6]3−octahedra as the function of temperature.This study paves the way towards a new class of smart windows and camouflage coatings with an unprecedented colour range based on a Cs_(2)NaFeCl_(6) perovskite. | Wenzhe Li Naveed Ur Rahman Yeming Xian Hang Yin Yunkai Bao Yi Long Songyang Yuan Yangyi Zhang Yaxuan Yuan Jiandong Fan | 2021 | Journal of Semiconductors2021，42，7： | 2 |
| 9 | 3D-printed NIR-responsive shape memory polyurethane/magnesium scaffolds with tight-contact for robust bone regeneration显示文摘Patients with bone defects suffer from a high rate of disability and deformity.Poor contact of grafts with defective bones and insufficient osteogenic activities lead to increased loose risks and unsatisfied repair efficacy.Although self-expanding scaffolds were developed to enhance bone integration,the limitations on the high transition temperature and the unsatisfied bioactivity hindered greatly their clinical application.Herein,we report a near-infrared-responsive and tight-contacting scaffold that comprises of shape memory polyurethane(SMPU)as the thermal-responsive matrix and magnesium(Mg)as the photothermal and bioactive component,which fabricated by the low temperature rapid prototyping(LT-RP)3D printing technology.As designed,due to synergistic effects of the components and the fabrication approach,the composite scaffold possesses a homogeneously porous structure,significantly improved mechanical properties and stable photothermal effects.The programmed scaffold can be heated to recover under near infrared irradiation in 60s.With 4 wt%Mg,the scaffold has the balanced shape fixity ratio of 93.6%and shape recovery ratio of 95.4%.The compressed composite scaffold could lift a 100 g weight under NIR light,which was more than 1700 times of its own weight.The results of the push-out tests and the finite element analysis(FEA)confirmed the tight-contacting ability of the SMPU/4 wt%Mg scaffold,which had a signficant enhancement compared to the scaffold without shape memory effects.Furthermore,The osteopromotive function of the scaffold has been demonstrated through a series of in vitro and in vivo studies.We envision this scaffold can be a clinically effective strategy for robust bone regeneration. | Yuanchi Zhang Cairong Li Wei Zhang Junjie Deng Yangyi Nie Xiangfu Du Ling Qin Yuxiao Lai | 2022 | Bioactive Materials2022，7，10： | 1 |
| 10 | Compact fluxgate magnetic full-tensor gradiometer with spherical feedback coil显示文摘 | Sui Yangyi Li Guang Wang Shilong | 2014 | Review of Scientific Instruments2014，85，01： | 1 |
| 11 | Asphericity errors correction of magnetic gradient tensor invariants method for magnetic dipole localization显示文摘 | Sui Yangyi Li Guang Wang Shilong | 2012 | IEEE Transactions on Magnetics2012，48，12： | 1 |
| 12 | Synthesis,crystal structure and photoluminescence properties of novel double perovskite La2CaSnO6:Eu3+red-emitting phosphors显示文摘A series of new double perovskite LaEuCaSnO(0≤x≤0.8)red phosphors were synthesized by traditional solid-state reaction.The phase,micro structure,photoluminescence(PL)properties,quantum efficiency,and thermal stability of the phosphors were investigated.LaCaSnOmatrix has a monoclinic double perovskite structure with space group P2/n.Under near-ultraviolet(UV)light at395 nm,LaEuCaSnOphosphors exhibit the most typical red emission peak at 614 nm,which corresponds to~5 D→~7 Felectric dipole transition of Eu.The optimum Eudoping content is attained at x=0.5,and the LaEuCaSnOphosphor shows a moderate quantum efficiency(32.3%)and high color purity(92.2%).Besides,the temperature-dependent spectrum of the phosphor was studied.The emission intensity of Euat 423 K decreases to 70.94%of the initial intensity at 303 K,and the activation energyΔE is estimated to be 0.232 eV,suggesting that the phosphors possess good thermal stability.The fabricated w-LED based on the phosphors has higher Ra(89),lower CCT(4539 K),and better chromaticity coo rdinates(0.371,0.428).These results prove that the Eu-doped LaCaSnOred phosphor has great potential applications in w-LEDs. | Yangyi Xu Guifang Li Xufeng Guan Yunge Wei Maolin Zhang | 2022 | Journal of Rare Earths2022，40，11： | 1 |
| 13 | Rice OsUBR7 modulates plant height by regulating histone H2B monoubiquitination and cell proliferation显示文摘Plant height is an important agronomic trait for lodging resistance and yield.Here,we report a new plantheight-related gene,OsUBR7 in rice(Oryza sativa L.);knockout of OsUBR7 caused fewer cells in internodes,resulting in a semi-dwarf phenotype.OsUBR7 encodes a putative E3 ligase containing a plant homeodomain finger and a ubiquitin protein ligase E3 component N-recognin 7(UBR7)domain.OsUBR7 interacts with histones and monoubiquitinates H2B(H2Bub1)at lysine148 in coordination with the E2 conjugase OsUBC18.OsUBR7 mediates H2Bub1 at a number of chromatin loci for the normal expression of target genes,including cell-cycle-related and pleiotropic genes,consistent with the observation that cell-cycle progression was suppressed in the osubr7 mutant owing to reductions in H2Bub1 and expression levels at these loci.The genetic divergence of OsUBR7 alleles among japonica and indica cultivars affects their transcriptional activity,and these alleles may have undergone selection during rice domestication.Overall,our results reveal a novel mechanism that mediates H2Bub1 in plants,and UBR7 orthologs could be utilized as an untapped epigenetic resource for crop improvement. | Yangyi Zheng Sensen Zhang Yanqiu Luo Fuquan Li Jiantao Tan Bin Wang Zhe Zhao Huifang Lin Tingting Zhang Jianhong Liu Xupeng Liu Jingxin Guo Xianrong Xie Letian Chen Yao-Guang Liu Zhizhan Chu | 2022 | Plant Communications2022，3，6： | 0 |
| 14 | N-doped graphitic carbon encapsulating cobalt nanoparticles derived from novel metal–organic frameworks for electrocatalytic oxygen evolution reaction显示文摘Nitrogen-doped carbon catalysts with hierarchical porous structure are promising oxygen evolution reaction(OER)catalysts due to the faster mass transfer and better charge carrying ability.Herein,an exquisite high nitrogen-containing ligand was designed and readily synthesized from the low-cost biomolecule adenine.Accordingly,three new MOFs(TJU-103,TJU-104 and TJU-105)were prepared using the Co(II)or Mn(II)ions as metal nodes.Through rationally controlling pyrolysis condition,in virtue of the high nitrogen content in well-defined periodic structure of the pristine MOFs,TJU-104–900 among the derived MOFs with hierarchical porous structure,i.e.,N-doped graphitic carbon encapsulating homogeneously distributed cobalt nanoparticles,could be conveniently obtained.Thanks to the synergistic effect of the hierarchical structure and well dispersed active components(i.e.,C=O,Co–Nx,graphitic C and N,pyridinic N),it could exhibit an overpotential of 280 mV@10mA/cm^(2)on carbon cloth for OER activity.This work provides the inspiration for fabrication of nitrogen-doped carbon/metal electrocatalysts from cost-effective and abundant biomolecules,which is promising for practical OER application. | Yuanmeng Tian Hao Wu Aamir Hanif Yanli Niu Ying Yin Yangyi Gu Zuofeng Chen Qinfen Gu Yun Hau Ng Jin Shang Liangchun Li Mingxian Liu | 2023 | Chinese Chemical Letters2023，34，8： | 0 |
| 15 | General synthesis of 2D rare-earth oxide single crystals with tailorable facets显示文摘Two-dimensional(2D)rare-earth oxides(REOs)are a large family of materials with various intriguing applications and precise facet control is essential for investigating new properties in the 2D limit.However,a bottleneck remains with regard to obtaining their 2D single crystals with specific facets because of the intrinsic non-layered structure and disparate thermodynamic stability of different facets.Herein,for the first time,we achieve the synthesis of a wide variety of high-quality 2D REO single crystals with tailorable facets via designing a hard-soft-acid-base couple for controlling the 2D nucleation of the predetermined facets and adjusting the growth mode and direction of crystals.Also,the facet-related magnetic properties of 2D REO single crystals were revealed.Our approach provides a foundation for further exploring other facet-dependent properties and various applications of 2D REO,as well as inspiration for the precise growth of other non-layered 2D materials. | Linyang Li Fangyun Lu Wenqi Xiong Yu Ding Yangyi Lu Yao Xiao Xin Tong Yao Wang Shuangfeng Jia Jianbo Wang Rafael GMendes Mark HRümmeli Shengjun Yuan Mengqi Zeng Lei Fu | 2022 | National Science Review2022，9，5： | 0 |
| 16 | The role of signaling crosstalk of microglia in hippocampus on progression of ageing and Alzheimer's disease显示文摘Based on single-cell sequencing of the hippocampi of 5×familiar Alzheimer's disease(5×FAD)and wild type mice at 2-,12-,and 24-month of age,we found an increased percentage of microglia in aging and Alzheimer's disease(AD)mice.Blood brain barrier injury may also have contributed to this increase.Immune regulation by microglia plays a major role in the progression of aging and AD,according to the functions of 41 intersecting differentially expressed genes in microglia.Signaling crosstalk between C−C motif chemokine ligand(CCL)and major histocompatibility complex-1 bridges intercellular communication in the hippocampus during aging and AD.The amyloid precursor protein(APP)and colony stimulating factor(CSF)signals drive 5×FAD to deviate from aging track to AD occurrence among intercellular communication in hippocampus.Microglia are involved in the progression of aging and AD can be divided into 10 functional types.The strength of the interaction among microglial subtypes weakened with aging,and the CCL and CSF signaling pathways were the fundamental bridge of communication among microglial subtypes. | He Li Tianyuan Ye Xingyang Liu Rui Guo Xiuzhao Yang Yangyi Li Dongmei Qi Yihua Wei Yifan Zhu Lei Wen Xiaorui Cheng | 2023 | Journal of Pharmaceutical Analysis2023，13，7： | 0 |
| 17 | Effects of β-mercaptoethanol Concentrations on Developmental Ability of Oocytes in 6-week-old Dorper Sheep显示文摘This study was conducted to investigate the effects of different concentrations ofβ-mercaptoethanol on the development ability of oocytes in Dorper sheep at the age of 6 weeks.In this study,6 Dorper sheep of 6 weeks old were chosen to induce oocyte collection in vivo by gonadotropin,and A and B grade cumulus oocyte complexes were obtained(COCs)with different concentrations of(0,50,70 and 100μmol/L)ofβ-mercaptoethanol in the mature liquid.The cleavage rate and blastocyst rate were counted after matured oocytes and capacitated sperm were co-incubated and fertilized eggs were cultivated.The results showed that compared with the cleavage rate(59.13%)and the blastocyst rate(12.17%)of the control group,the cleavage rates(60.87%,63.48%and 65.22%)and blastocyst rates(14.78%,17.39%and 21.74%)of the test groups I,II and III were higher(P>0.05),and the order was test group III>test group II>test group I>control group.The cleavage rate and blastocyst rate of the 100μmol/Lβ-mercaptoethanol group increased by 6.09 percentage points and 9.57 percentage points,respectively.It indicated that the addition of a certain amount ofβ-mercaptoethanol in the mature liquid could improve the developmental ability of oocytes and the quality of fertilized eggs of the 6-week-old Doper sheep.When theβ-mercaptoethanol concentration was within 100μmol/L,the cleavage rate and the blastocyst rate of oocytes were on the rise.The optimal concentration ofβ-mercaptoethanol in the mature liquid still needs further study. | Yangyi MAO Huidi LUO Zhiwu WANG Peng ZHAO Guanwu ZHANG Jun LI Hongyu GUO Li ZHANG Lina XUE Wenqing DANG | 2019 | Agricultural Biotechnology2019，8，4： | 0 |
| 18 | Systematic co-delivery of dual agonists to enhance cancer immunotherapy显示文摘In the tumor immunosuppressive microenvironment(TIME),antigen presenting cells(APCs)usually exhibit a tumor suppressor phenotype.Toll-like receptors(TLRs)agonists could reprogram M2-type macrophages to M1-type and stimulate dendritic cells(DCs)maturation.The combination of TLR7/8 and TLR9 agonists seems to have synergistic therapeutic efficacy.Here,we designed a lipid-coated mesoporous silica nanoparticle(MSNs@Lipo)for the co-delivery of TLR7/8 agonist resiquimod(R848)and TLR9 agonist CpG oligodeoxynucleotides(ODNs)(CpG@MSNs-R@L-M).R848 was firstly conjugated onto the nanoparticle via silane chemistry,which is acidic responsive drug release.Then,CpG was loaded onto the nanoparticle through the positive charge mainly from TLR7/8 agonist R848.Our in vitro experiments further indicated that both drugs have acid-responsive release properties and could be taken up by DCs and located on the endosomes of APCs.More importantly,CpG@MSNs-R@L-M could significantly improve the antitumor efficacy in B16F10 melanoma model.The mechanistic study demonstrated that CpG@MSNsR@L-M could remarkably modulate the TIME by promoting the maturation of DCs and repolarizing macrophages from M2 to M1 phenotype and facilitating the infiltration of tumor cytotoxic T cells.It was concluded that in comparison to single agonist,the codelivery of dual agonists,CpG and R848,can improve anti-tumor immune responses for cancer immunotherapy. | Xiangxia Li Guiyuan Chen Yangyi Wang Lanhong Su Bo Chen Kecheng Wu Yun Xing Zechenxi Song Ruike Dai Tianxin Liu Jiaao Zhao Zhe Xie Peijie Zhou Xiaoping Xia Yuanzeng Min | 2022 | Nano Research2022，15，9： | 0 |
| 19 | 比较两组连续性结果的样本计算显示文摘概述:所有的临床研究都需要对样本量进行辨证。然而,对于众多生物医学和临床研究人员来说,把握度和样本量看起来就像一个统计学家的魔术。在本文中,我们讨论了把握度和样本量的计算,并说明生物医学和临床研究人员在该分析的可行性和意义中具有重要作用。因此,把握度分析的确是一个互动的过程,并且科学研究人员和统计人员在研究团队中是平等合作的伙伴。 | Julia Z. ZHENG Yangyi LI Tuo LIN Angelica ESTRADA Xiang LU Changyong FENG | 2017 | 上海精神医学2017，29，4： | 0 |
| 20 | Study on In-vitro Production Technology of Embryos from Young Dorper Sheep显示文摘This study was conducted to investigate the in-vitro production technology of embryos from young Dorper sheep, so as to provide technical support for the utilization of ovarian follicles in young Dorper sheep. Tests were conducted from the induction of Dorper sheep of 4 to 8 weeks old using follicle stimulating hormone(FSH) and pregnant horse serum(PMSG), collection of oocytes, in-vitro oocyte maturation-fertilization-zygote cultivation and 2-4-cell-stage fertilized ovum transfer. The results showed that 585 oocytes were collected from eight Dorper sheep at the age of 4 and 8 weeks, with an average of 73.13 oocytes/sheep. 346 of the 2-4-cell-stage fertilized eggs were obtained, whose cleavage rate was 59.15%. 77 in-vitro fertilized eggs at 2-4-cell stage were transplanted into 17 recipient sheep, seven of which were pregnant and gave birth to 13 'test-tube sheep' with a conception rate of 41.18%. It is indicated that the hormone induction technique, in-vitro oocyte maturation-fertilization-zygote cultivation technique and 2-4-cell-stage fertilized ovum transfer technique used in this study can serve as effective techniques for the in-vitro production of embryos from Dorper sheep of 4-8 weeks old. | Huidi LUO Yangyi MAO Huihui GUO Li ZHANG Zhiwu WANG Zhijun WANG Jun LI Hongyu GUO Lina XUE Wenqing DANG | 2019 | Agricultural Biotechnology2019，8，6： | 0 |
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