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| 1 | Efficacies of β-L-D4A against Hepatitis B virus in 2.2.15 cells显示文摘AIM:To investigate the antiviral effect of beta-L-enantiomer of 2',3'-didehydro-2',3'-dideoxyadenosine (β-L-D4A) on 2.2.15 cells transfected with the hepatitis B virus (HBV) genome. METHODS:Lamivudine (3TC) as a positive control. Then, HBV DNA in treated 2.2.15 cells and the Hepatitis B surface antigen (HBsAg) in the culture supernatants were detected to determine the inhibitory effect of β-L-D4A. At the same time, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) was used to detect the survival ratio of 2.2.15 cells. RESULTS:β-L-D4A has a dose-dependent inhibitory effect on HBV DNA replication;this effect was apparent when the concentration was above 1 mol/L. When β-L-D4A was at the highest concentration, 100 mol/L, the HBsAg inhibition ratio was above 50%. The Therapeutic index (TI) of β-L-D4A was above 2.1. CONCLUSION:β-L-D4A has a dose-dependent inhibitory effect on the replication of HBV DNA and the secretion of HBsAg at low toxicity. | Lin-Lin Gao, Xiao-Yan Wang, Ju-Sheng Lin, Ying-Hui Zhang, Yan Li, Institute of Liver Diseases, Tongji Hospital, Tongii Medical College, Huazhong University of Science and Technology, Wuhan 430030, Hubei Province, China Author contributions:Gao LL and Wang XY contributed equally to this work Gao LL, Wang XY, Lin JS, Zhang YH, Li Y designed the research Gao LL and Wang XY performed the research Gao LL wrote the paper | 2008 | World Journal of Gastroenterology2008，14，8： | 7 |
| 2 | Soil fungistasis and its relations to soil microbial composition and diversity:A case study of a series of soils with different fungistasis显示文摘Fungistasis is one of the important approaches to control soil-borne plant pathogens.Some hypotheses about the mechanisms for soil fungistasis had been established,which mainly focused on the soil bacterial community composition,structure,diversity as well as function.In this study,the bacterial community composition and diversity of a series of soils treated by autoclaving,which coming from the same original soil sample and showing gradient fungistasis to the target soil-borne pathogen fungi Fusarium gr... | Wu Minna Zhang Huiwen Ll Xinyu Zhang Yan Su Zhencheng Zhang Chenggang | 2008 | Journal of Environmental Sciences2008，20，7： | 7 |
| 3 | Transcriptmne analysis of skin pho- toaging in chinese females reveals the involvement of skin home- ostasis and metabolic change显示文摘 | Yan W Zhang LL Yan L | 2013 | PLoS One2013，8，61： | 1 |
| 4 | Psychosocial factors and risk of hypertension: the coronary artery risk development in young adults (CARDIA) study显示文摘 | Yan LL Liu K Matthews KA | 2003 | JAMA2003，290，16： | 1 |
| 5 | Molecular epidemiology ofrabies in Fujian Province,southeast of China显示文摘 | Zhang JM Yan YS Wang LL | 2009 | Emerg InfectDis2009，15，8： | 1 |
| 6 | Potency fingerprint of herbal products danshen injection for their quality evaluation 显示文摘 | Chang YX Yan DM Chen LL | 2009 | Chem Pharm Bull2009，57，6： | 1 |
| 7 | Postiridotomy ultra- sound biomicroscopy features in the fellow eye of Chinese patients with acute primary angle-closure and chronic pri- mary angle-closure glaucoma显示文摘 | CHEN HJ WANG X YAN YJ WU LL | 2015 | J Glaucoma2015，24，3： | 1 |
| 8 | Rhizopus chinensis lipase:Gene cloning,expression in Pichia pastoris andproperties显示文摘 | Yu XW Wang LL Yan X | 2009 | Journal of Molecular Catalysis B:Enzymatic2009，57，14： | 1 |
| 9 | Long-term effects of recurrent neonatal seizures on neurobehavioral function and related gene expression and its intervention by inhibitor of Cathepsin B显示文摘 | Ni H Yan JZ Zhang LL | | 0，，01： | 1 |
| 10 | Treatment of large impacted proximal ureteral stones:randomized comparison of minimally invasive percutaneous antegrade ureterolithotripsy versus retrograde ureterolithotripsy显示文摘 | Xiao-Jian G Jian LL Yan X | 2013 | World J Urol2013，，： | 1 |
| 11 | Antimyein A18 produced by an endophytic Streptomyces albidoflavus isolated from a mangrove plant显示文摘 | Yan LL Han NN Zhang YQ | 2010 | The Journal of Antibiotics2010，63，5： | 1 |
| 12 | Self-reported and measured sleep duration: how similar are they?显示文摘 | Lauderdale OS Knutson KL Yan LL | 2008 | Epidemiology2008，19，6： | 1 |
| 13 | Stability of the Pittsburgh sleep qual- ity index and the Epworth sleepiness questionnaires over 1 year in early middle-aged adults: the CARDIA study显示文摘 | Knuson KLRathouz PJ Yan LL | 2006 | Sleep2006，29，： | 1 |
| 14 | Psychosocial factors and risk of hypertension显示文摘 | Yan LL Liu K Matthews KA | 2003 | JAMA2003，290，16： | 1 |
| 15 | Psychosocial factors and risk of hypertension:the coronary artery risk development in young adults(CARDIA)study显示文摘 | Yan LL Liu K Mattews KA | 2003 | JAMA2003，290，： | 1 |
| 16 | HMW-GS accumulation and GMP particles size distribution in wheat grain in relation to nitro- gen rate 显示文摘 | Ll W Yan S Shi X | 2013 | Journal of Food Agriculture and Environ- ment2013，11，： | 1 |
| 17 | Fluoride treatment and in vitro corrosion behavior of an AZSIB magnesium alloy 显示文摘 | Yan TT Tan LL Xiong DS | 2010 | Mater Sci Engineer C2010，30，5： | 1 |
| 18 | Long-term effects of recurrent neonatal seizures on neurobehavioral function and related gene expression and its intervention by inhibitor of cathepsin B显示文摘 | Ni H Yan JZ Zhang LL | | 0，，01： | 1 |
| 19 | Blood pressure differences be- tween northern and southern Chinese: role of dietary factors: the international study on macronutrients and blood pressure 显示文摘 | Zhao L Stamler J Yan LL | 2004 | Hypertension2004，43，6： | 1 |
| 20 | Study of mechanism of differentiation of bone stromal stem cells into neurons in vitro显示文摘 | Yan BB Wang LL Qiao JF | 2005 | J Cell Mol Immunol2005，21，3： | 1 |
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