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| 1 | 分子生物学方法在微生物生态学中的应用显示文摘给出了植被对微生物群落结构影响的研究结果,同时较详细地介绍了从土壤样品中提取DNA以及对DNA进行克隆,并获得微生物克隆群落的方法和步骤.应用该方法在获得高产量DNA的同时,获得了多样性极高的微生物克隆群落. | 夏北成 Zhou,JZ | 1998 | 中山大学学报(自然科学版)1998，37，2： | 31 |
| 2 | Nitric oxide synthase distribution in esophageal mucosa and hemodynamic changes in rats with cirrhosis显示文摘AIM To observe the nitric oxide synthase (NOS) distribution in the esophageal mucosa andhemodynamic changes in cirrhotic rats.METHODS NOS distribution in the loweresophagus of rats with carbon tetrachlorideinduced cirrhosis was assessed by using NADPHdiaphorase (NADPH-d ) histochemical method.Concentration of NO in serum were measured byfluorometric assay. Mean arterial pressure(MAP), cardiac output (CO), cardiac index (CI),splanchnic vascular resistance (SVR ), andsplanchnic blood flow (SBF ) were alsodetermined using 5’CO-labeled microspheretechnique.RESULTS Intensity of NOS staining in theesophageal epithelium of cirrhotic rats wassignificantly stronger than that in controls.There was a NOS--positive staining area in theendothelia of esophageal submucosal vessels ofcirrhotic rats, but the NOS staining was negativein normal rats. NO concentration of serum incirrhotic rats were significantly higher incomparison with that of controls. Cirrhotic ratshad significantly lower MAP, SVR and higherSBF than those of the controls.CONCLUSION SPlanchnic hyperdynamiccirculatory state was observed in rats withcirrhosis. The endogenous NO may play animportant role in development of esophagealvarices and in changes of hemodynamics incirrhosis. | Huang YQ Xiao SD Zhang DZ Mo JZ | 1999 | World Journal of Gastroenterology1999，5，3： | 22 |
| 3 | 急性缺血性卒中静脉阿替普酶溶栓后出血转化的治疗和结局:美国心脏协会/美国卒中协会科学声明显示文摘急性缺血性卒中发病4.5 h内给予静脉阿替普酶溶栓治疗可改善预后。尽管阿替普酶的有效性被证实,但因增加出血风险,尤其是症状性颅内出血(symptomatic intracranial hemorrhage,sICH),其应用受到限制。sICH的发生率在不同患者和不同sICH定义中不同,一般波动于2%~7%。 | Yaghi S Willey JZ Cucchiara B 蒋芳 赵文博 吴川杰 李传辉 任明 吉训明 | 2018 | 中国脑血管病杂志2018，15，7： | 17 |
| 4 | Aminoglycoside-induced and non-syndromic hearing loss is associated with the G7444A mutation in the mitochondrial COI/tRNA (Ser(UCN)) genes in two Chinese families显示文摘 | Zhu Y Qian YP Tang XW Wang JD Yang L Liao ZS Li RH Ji JZ Li ZY Chen JF Choo DI Lu JX Guan MX | 2006 | 中国生物学文摘2006，20，4： | 16 |
| 5 | Effects of endogenous beta-amyloid overproduction on tau phosphorylation in cell culture.显示文摘 | Wang ZF Li HL Li XC Zhang Q Tian Q Wang Q Xu H Wang JZ | 2006 | 中国生物学文摘2006，20，9： | 15 |
| 6 | Cultivation of human liver cell lines with microcarriers acting as biological materials of bioartificial liver显示文摘AIM To improve the Cultivation efficiency and yield of human liver cell line CL--l.METHODS High--density cultivation of CL--l onmicrocarriers was carried out with periodicobservation of their growth and proliferation.The specific functions of human liver cell werealso determined.RESULTS Cells of CL-1 cell line grew well onmicrocarrier Cytodex--3 and on the 7th day thepeak was reached. The amount of CLI cells was2.13 x 10’ and the total amount of albuminsynthesis reached 71 .23 ig, urea synthesis23 .32 mg and diazepam transformation 619 .7 ugrespectively. The yield of CL-l on microcarrierswas 49.3 times that of conventional cultivation.The amounts of albumin synthesis, ureasynthesis and diazepam transformation were39.8 times, 41 .6 times and 33.3 times those ofconventional Cultivation, respectively.CONCLUSION The human liver cell line CL--1 canbe Cultivated to a high density with Cytodex--3and has better biological functions. High-densityCultivation of CLI on microcarriers can act asthe biological material of bioartificial liver. | Gao Y Xu XP Hu HZ Yang JZ | 1999 | World Journal of Gastroenterology1999，5，3： | 13 |
| 7 | Cloning and sequence analysis of human genomic DNA of augmenter of liver regeneration显示文摘INTRODUCTIONThe liver is one of the organs,which have potentialregenerative capability in mammalian animal.The study of the canine model indicated that theliver could regenerate to original size after 70%hepatectomy in only two weeks.So it is a hotresearch topic for the cellular and molecularmechanism of liver regeneration. | Cheng J Zhong YW Liu Y Dong J Yang JZ Chen JM | 2000 | World Journal of Gastroenterology2000，6，2： | 13 |
| 8 | Concurrent alterations of O-GlcNAcylation and phosphorylation of tau in mouse brains during fasting.显示文摘 | Li X Lu F Wang JZ Gong CX | 2006 | 中国生物学文摘2006，20，9： | 12 |
| 9 | Luminescence Properties of the Ternary Terbium Complexes with (Ortho and Para) Aminobenzoic acids and 1,10-Phenanthroline Incorporated in Silica Matrix by a Sol-gel Method显示文摘Ternary complexes of terbium with ortho (and para) aminobenzoic acid and 1.10 phenanthroline were introduced into silica gel by the sol-gel method. The luminescence behavior of the solid-state samples was studied during the sol-gel aging process by means of emission, excitation spectra, lifetimes and quantum efficiencies. | Yan, B Zhang, HJ Wang, SB Ni, JZ | 1997 | Chinese Chemical Letters1997，8，7： | 10 |
| 10 | Microarray-based analysis of changes in diversity of microbial genes involved in organic carbon decomposition following land use/cover changes显示文摘 | Zhang YG Zhang XQ Liu XD Xiao Y Qu L J Wu LY Zhou JZ | 2007 | 中国生物学文摘2007，21，8： | 10 |
| 11 | Role of melatonin in Alzheimer-like neurodegeneration显示文摘 | Wang JZ Wang ZF | 2006 | 中国生物学文摘2006，20，10： | 9 |
| 12 | Activation of glycogen synthase kinase-3 induces Alzheimer-like tau hyperphosphorylation in rat hippocampus slices in culture.显示文摘 | Li X Lu F Tian Q Yang Y Wang Q Wang JZ | 2006 | 中国生物学文摘2006，20，9： | 9 |
| 13 | Preliminary study on light hydrocarbons in ore-forming fluids of gold and antimony deposits in southwestern Guizhou, China显示文摘METAL minerals precipitate generally from ore-forming fluid. It is suggested that organic mat-ter in ore-forming fluid is one of the best media which show the role of organic matter in oregenesis. Because mineralizing temperatures of many ore deposits exceeded the stable rangesof liquid hydrocarbons, only light hydrocarbons were preserved well in inclusions. Thus, | Zhuang, HP Lu, JL Fu, JM Liu, JZ Shi, JX | 1997 | Chinese Science Bulletin1997，42，20： | 8 |
| 14 | Primary porcine hepatocytes with portal vein serum cultured on microcarriers or in spheroidal aggregates显示文摘AIM To develop a culture mode providingdurable biomaterials with high yields andactivities used in bioartificial liver.METHODS Hepatocytes were isolated from awhole pig liver by Seglen’s method of orthotopicperfusion with collagenase.In culture onmicrocarriers,primary porcine hepatocyteswere inoculated at a concentration of 5×10~7/mLinto the static culture systems containing 2 g/LCytodex-3,then supplemented with 100 mL/Lfetal calf serum(FCS)or 100 mL/L porcineportal vein serum(PPVS)respectively.Inspheroidal aggregate culture hepatocytes wereinoculated into 100 mL siliconized flasks at aconcentration of 5.0×10~6/mL.RESULTS In culture on microcarriershepatocytes tended to aggregate on Cytodex-3obviously after being inoculated.Typical multi-cellular aggregated spheroids could be found inthe two systems 24 h-48 h after hepatocyteswere cultured.The morphological charact-eristics and synthetic functions were maintainedfor 5 wk in FCS culture system and 8 wk in PPVSculture system.In spheroidal aggregate cultureabout 80%-90% isolated hepatocytes becameaggregated spheroids 24h after cultured insuspension and mean diameter of the spheroidswas 100μm.The relationship among thehepatocytes resembled that in the liver in vivo.Synthetic functions of albumin and urea of the spheroids were twice those of hepatocytescultured on monolayers.CONCLUSION As high-yields and high-activitymodes of culture on microcarriers or inspheroidal aggregate culture with portal veinserum are promising to provide biomaterials forbioartificial liver(BAL)efficiently. | Gao Y Hu HZ Chen K Yang JZ | 2000 | World Journal of Gastroenterology2000，6，3： | 6 |
| 15 | Mechanical Behaviour and Microstructure Evolution of Superplastic Mg-8.4 wt pct Li Alloy and Effect of Grain Size and Phase Ratio on Its Elongation显示文摘Mechanical properties and microstructure evolution of Mg-8.4 wt pct Li alloy have been investi- gated. The superplastic elongation-to-failure 920% at 573 K and initial strain rate of 5 × 10-4 s-1 at low nominal stress 2 MPa has been demonstrated. It was shown through optical microstructure that the average grain sizes at gauge length section before and after superplastic tension and at grip section were 7.5 μm, 31.7 μm and 20 μm respectively, indicating significant deformation induced grain growth and static grain growth. Comparison of experiment data with published data for superplastic Mg-Li alloys shows that fine grain size and about 50 to 50 phase ratio contribute to achieving the largest superplasticity. Some related research works in other alloys are compiled and further evidences to such theoretic argument are provided. | Cao, FR Cui, JZ Wen, JL Lei, F | 2000 | Journal of Materials Science & Technology2000，16，1： | 5 |
| 16 | Cyclic adenosine 3',5'-monophosphate induces differentiation of mouse embryonic stem cells into cardiomyocytes显示文摘 | Chen Y Shao JZ Xiang LX Guo J Zhou Q J Yao X Dai LC Lu YL | 2006 | 中国生物学文摘2006，20，7： | 5 |
| 17 | Endogenous overproduction of beta-amyloid induces tau hyperphosphorylation and decreases the solubility of tau in N2a cells.显示文摘 | Wang YP Wang XC Tian Q Yang Y Zhang Q Zhang JY Zhallg YC Wang ZF Wang Q Li H Wang JZ | 2006 | 中国生物学文摘2006，20，9： | 5 |
| 18 | Quantum chemical studies on antimalarial of artemisinin (qinghaosu) derivatives显示文摘In this paper theoretical studies were performed on artemisinin (qinghaosu) derivatives with semiempirical quantum chemical methods AMI and PM3. The antimalarial activity -logC has an obvious correlation with the net charge of C(16) and bond orders of bonds O(1)-C(10), O(2)-C(6), O(1)-O(2) and O(5)-C(16). According to the calculation results, we derived structure-activity relationship, presented the probable pharmacophore of qinghaosu derivatives and the interaction fashion between the drugs and the plasmodium receptor. | JIANG, HL CHEN, KX TANG, Y CHEN, JZ JI, RY | 1995 | Chinese Journal of Chemistry1995，13，2： | 5 |
| 19 | TRH microinjection into DVC enhances motility of rabbits gallbladder via vagus nerve显示文摘TRHmicroinjectionintoDVCenhancesmotilityofrabbitsgalbladderviavagusnerveLIUChuanYong,LIUJingZhang,ZHOUJianHua,WANGHanRu,... | Liu, CY Liu, JZ Zhou, JH Wang, HR Li, ZY Li, AJ Liu, KJ | 1998 | World Journal of Gastroenterology1998，4，2： | 4 |
| 20 | A transitory activation of protein kinase-A induces a sustained tau hyperphosphorylation at multiple sites in N2a cells-imply a new mechanism in Alzheimer pathology.显示文摘 | Zhang Y Li HL Wang DL Liu S J Wang JZ | 2006 | 中国生物学文摘2006，20，9： | 4 |
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