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| 1 | An in vivo study with an MRI tracer method reveals the biophysical properties of interstitial fluid in the rat brain显示文摘The nature of brain interstitial fluid (ISF) has long been a subject of controversy. Most of the previous studies on brain ISF were carried out in vitro. In the present study, a novel method was developed to characterize ISF in the living rat brain by magnetic resonance (MR) imaging using gadolinium-diethylenetriaminepentaacetic acid (Gd-DTPA) as a tracer. Sprague Dawley rats (n=8) were subjected to MR scanning before and after the introduction of Gd-DTPA into the caudate nucleus. A one-way drainage of brain ISF was demonstrated on the dynamic MR images. According to the traditional diffusion model, the diffusion and clearance rate constants of the tracer within brain extracellular space (ECS) were derived as (3.38±1.07)×10-4 mm 2 s-1 and (7.60±4.18)×10-5 s-1 . Both diffusion and bulk flow contributed to the drainage of ISF from the caudate nucleus, which demonstrated an ISF-cerebrospinal fluid confluence in the subarachnoid space at the lateral and ventral surface of the brain cortex at 3 h after the injection. By using this newly developed method, the brain ECS and ISF can be quantitatively measured simultaneously in the living brain, which will enhance the understanding of ISF and improve the efficiency of drug therapy via the brain interstitium. | HAN HongBin LI Kai YAN JunHao ZHU Kai FU Yu | 2012 | Science China(Life Sciences)2012，55，9： | 25 |
| 2 | VGLL4 functions as a new tumor suppressor in lung cancer by negatively regulating the YAP-TEAD transcriptional complex显示文摘肺癌症是与高发生和死亡世界范围的大多数破坏疾病之一。河马(Hpo ) 小径是在果蝇和哺乳动物的机关尺寸的一个保存管理者。新兴的证据在癌症开发建议了 Hpo 小径的意义。在这研究,我们通过否定地调整 YAP-TEAD 建筑群的形成在肺 carcinogenesis 作为新奇肿瘤 suppressor 识别 VGLL4, Hpo 的核心部件小径。我们 VGLL4 经常被观察低层的数据表演在老鼠和人的肺癌症标本表示了。VGLL4 的宫外的表示显著地在 vitro 压制肺癌症房间的生长。更重要地, VGLL4 显著地在 de novo 老鼠模型禁止肺癌症前进。我们进一步发现 VGLL4 禁止 YAP-TEAD transcriptional 建筑群的活动。我们 VGLL4 直接在绑定与笨蛋竞争到 TEAD 并且执行的数据表演它通过二 TDU 域的生长禁止的函数。一起,我们的学习证明 VGLL4 是为通过否定地调整 YAP-TEAD 复杂形成并且这样的肺癌症的新奇肿瘤 suppressor Hpo 小径。 | Wenjing Zhang Yijun Gao Peixue Li Zhubing Shi Tong Guo Fei Li Xiangkun Han Yan Feng Chao Zheng Zuoyun Wang Fuming Li Haiquan Chen Zhaocai Zhou Lei Zhang Hongbin Ji | 2014 | Cell Research2014，24，3： | 25 |
| 3 | Identification of RET gene fusion by exon array analyses in "pan-negative" lung cancer from never smokers显示文摘 | Li, Fei Feng, Yan Fang, Rong Fang, Zhaoyuan Xia, Jufeng Han, Xiangkun Liu, Xin-Yuan Chen, Haiquan Liu, Hongyan Ji, Hongbin | 2012 | Cell Research2012，22，5： | 11 |
| 4 | Regulation network and expression profiles of Epstein-Barr virus-encoded microRNAs and their potential target host genes in nasopharyngeal carcinomas显示文摘Epstein-Barr virus(EBV)is associated with nasopharyngeal carcinoma(NPC)tumorigenesis.However,the mechanism(s)connecting EBV infection and NPC remain unclear.Recently,a new class of EBV microRNAs(miRNAs)has been described.To determine how EBV miRNAs control the expression of host genes,and to understand their potential role in NPC tumorigenesis,we profiled the expression of 44 mature EBV miRNAs and potential host genes in NPC and non-tumor nasopharyngeal epithelial tissues.We found that 40 EBV miRNAs from the BART transcript were highly expressed in NPC.Analysis of potential BART miRNA target genes revealed that 3140 genes and several important pathways might be involved in the carcinogenesis of NPC.A total of 105 genes with potential EBV miRNA binding sites were significantly downregulated,suggesting that EBV miRNAs may regulate these genes and contribute to NPC carcinogenesis.An EBV miRNA and host gene regulation network was generated to provide useful clues for validating of EBV miRNA functions in NPC tumorigenesis. | ZENG ZhaoYang HUANG HongBin HUANG LiLi SUN MengXi YAN QiJia SONG YaLi WEI Fang BO Hao GONG ZhaoJian ZENG Yong LI Qiao ZHANG WenLing LI XiaYu XIANG Bo LI XiaoLing LI Yong XIONG Wei LI GuiYuan | 2014 | Science China(Life Sciences)2014，57，3： | 10 |
| 5 | dentification of TRA2B-DNAH5 fusion as a novel oncogenic driver in human lung squamous cell carcinoma显示文摘 | Fei Li Zhaoyuan Fang Jian Zhang Chen Li Hongyan Liu Jufeng Xia Hongwen Zhu Chenchen auo Zhen Qin Fuming Li Xiangkun Han Yuetong Wang Yan Feng Ye Wang Wenjing Zhang Zuoyun Wang Yujuan Jin Yihua Sun Wenyi Wei Rong Zeng Haiquan Chen Hongbin Ji | 2016 | Cell Research2016，26，10： | 9 |
| 6 | Distribution of green algae micro-propagules and their function in the formation of the green tides in the coast of Qinhuangdao,the Bohai Sea, China显示文摘Since 2015, a novel green tide has been recurring in the coastal areas of Qinhuangdao at the western coast of the Bohai Sea in China, threatening the environment and ecosystem of the Beidaihe seaside holiday resort along the coast. Micro-propagules of the green algae including gametes, spores, micro-germlings and micro-vegetative fragments play an important role in the formation of green tides. They serve as a 'seed source' of green macroalgae, and their distributions could reflect and influence the 'algae source' of green tides. In this study,monthly surveys in the inshore and offshore areas of the Qinhuangdao coast were conducted from April to September 2016 and in January 2017 to investigate the tempo-spatial distribution patterns and the biomass variations of the green algae micro-propagules. The obtained results show that micro-propagules were mainly distributed in the inshore areas with a significantly decreasing abundance towards offshore areas. Their biomass was highest in July and August, and lowest in winter. The areas that were affected by the green tides showed a remarkably higher abundance of micro-propagules compared to other areas. These micro-propagules could serve as the 'seed' source of green tides. Their distribution patterns indicate that the green tide in the coastal areas of Qinhuangdao originated locally. | Hongbin Han Wei Song Zongling Wang Dewen Ding Chao Yuan Xuelei Zhang Yan Li | 2019 | Acta Oceanologica Sinica2019，38，8： | 8 |
| 7 | miR-375 is highly expressed and possibly transactivated by achaete-scute complex homolog 1 in small-cell lung cancer cells显示文摘在这研究,我们鉴别五 miRNAs 高度在小房间的肺癌症(SCLC ) 表示了房间线 NCI-H209。在他们之中, miR-375 的表示层次戏剧性地在检验的所有 SCLC 房间线被提高,与抄写因素 achaete 鳞甲建筑群相当或相同的事物的表示重合 1 (ASCL1 ) 。而且, miR-375 是 upregulated 并且在也从老鼠象 SCLC 一样肿瘤产生的房间线与 ASCL1 相关。双酶的试金进一步证明 ASCL1 在 miR-375 倡导者由绑定激活 miR-375 的表达式到三个电子盒子元素。这些结果经由 miR-375 的 upregulation 在 SCLC 暗示 ASCL1 的一个角色。 | Huijie Zhao Lei Zhu Yujuan Jin Hongbin Ji Xiumin Yan Xueliang Zhu | 2012 | Acta Biochimica et Biophysica Sinica2012，44，2： | 6 |
| 8 | Origin of strong solid solution strengthening in the CrCoNi-W medium entropy alloy显示文摘Solid solution strengthening is one of the most conventional strategies for optimizing alloys strength,while the corresponding mechanisms can be more complicated than we traditionally thought specifically as heterogeneity of microstructure is involved.In this work,by comparing the change of chemical distribution,dislocation behaviors and mechanical properties after doping equivalent amount of tungsten(W)atoms in CrCoNi alloy and pure Ni,respectively,it is found that the alloying element W in CrCoNi alloy resulted in much stronger strengthening effect due to the significant increase of heterogeneity in chemical distribution after doping trace amount of W.The large atomic scale concentration fluctuation of all elements in CrCoNi-3W causes dislocation motion via strong nanoscale segment detrapping and severe dislocation pile up which is not the case in Ni-3W.The results revealed the high sensitivity of elements distribution in multi-principle element alloys to composition and the significant consequent influence in tuning the mechanical properties,giving insight for complex alloy design. | Yujie Chen Yan Fang Xiaoqian Fu Yiping Lu Sijing Chen Hongbin Bei Qian Yu | 2021 | Journal of Materials Science & Technology2021，，14： | 5 |
| 9 | Engineering an enthesis-like graft for rotator cuff repair:An approach to fabricate highly biomimetic scaffold capable of zone-specifically releasing stem cell differentiation inducers显示文摘Rotator cuff(RC)attaches to humerus across a triphasic yet continuous tissue zones(bone-fibrocartilage-tendon),termed“enthesis”.Regrettably,rapid and functional enthesis regeneration is challenging after RC tear.The existing grafts bioengineered for RC repair are insufficient,as they were engineered by a scaffold that did not mimic normal enthesis in morphology,composition,and tensile property,meanwhile cannot simultaneously stimulate the formation of bone-fibrocartilage-tendon tissues.Herein,an optimized decellularization approach based on a vacuum aspiration device(VAD)was developed to fabricate a book-shaped decellularized enthesis matrix(O-BDEM).Then,three recombinant growth factors(CBP-GFs)capable of binding collagen were synthesized by fusing a collagen-binding peptide(CBP)into the N-terminal of BMP-2,TGF-β3,or GDF-7,and zone-specifically tethered to the collagen of O-BDEM to fabricate a novel scaffold(CBP-GFs/O-BDEM)satisfying the above-mentioned requirements.After ensuring the low immunogenicity of CBP-GFs/O-BDEM by a novel single-cell mass cytometry in a mouse model,we interleaved urine-derived stem cell-sheets into this CBP-GFs/O-BDEM to bioengineer an enthesis-like graft.Its high-performance on regenerating enthesis was determined in a canine model.These findings indicate this CBP-GFs/O-BDEM may be an excellent scaffold for constructing enthesis-like graft to patch large/massive RC tears,and provide breakthroughs in fabricating graded interfacial tissue. | Can Chen Qiang Shi Muzhi Li Yang Chen Tao Zhang Yan Xu Yunjie Liao Shulin Ding Zhanwen Wang Xing Li Chunfeng Zhao Lunquan Sun Jianzhong Hu Hongbin Lu | 2022 | Bioactive Materials2022，7，10： | 4 |
| 10 | Luminescence tuning of Ce^3+,Pr^3+activated(Y,Gd)AGG system by band gap engineering and energy transfer显示文摘Tuning of phosphor luminescence properties,including the emission energy/intensity and thermal stability,is an important way to develop superior luminescent materials for diverse applications.In this work,we discuss the effect of band gap engineering and energy transfer on the luminescence properties of Ce^3+or Pr^3+doped(Y,Gd)AGG systems,and analyze the underlying reasons for their different phenomena.By using VUV-UV excitation spectra and constructing VRBE schemes,the changes of host band structure,5 d excited level energies and emission thermal stability of Ce^3+and Pr^3+with the incorporation of Gd^3+ions were studied.In addition,the energy transfer dynamics was also investigated in terms of the luminescence decay curves.This work demonstrates a way to tune phosphor luminescence properties by combining band gap engineering and energy transfer tailoring and provides an inspiring discussion on the different results of Ce^3+doping on the Ce^3+and Pr^3+emissions. | Yiyi Ou Weijie Zhou Fengkai Ma Chunmeng Liu Rongfu Zhou Fang Su Yan Huang Pieter Dorenbos Hongbin Liang | 2020 | Journal of Rare Earths2020，38，5： | 3 |
| 11 | Iterative learning control for nonlinear systems with uncertain state delay and arbitrary initial error显示文摘Most of the existing iterative learning control algorithms proposed for time-delay systems are based on the condition that the time-delay is precisely available, and the initial state is reset to the desired one or a fixed value at the start of each operation, which makes great limitation on the practical application of corresponding results. In this paper, a new iterative learning control algorithm is studied for a class of nonlinear system with uncertain state delay and arbitrary initial error. This algorithm needs to know only the boundary estimation of the state delay, and the initial state is updated, while the convergence of the system is guaranteed. Without state disturbance and output measurement noise, the system output will strictly track the desired trajectory after successive iteration. Furthermore, in the presence of state disturbance and measurement noise, the tracking error will be bounded uniformly. The convergence is strictly proved mathematically, and sufficient conditions are obtained. A numerical example is shown to demonstrate the effectiveness of the proposed approach. | Hongbin WANG Yan WANG | 2011 | 控制理论与应用(英文版)2011，9，4： | 3 |
| 12 | The contribution of attached Ulva prolifera on Pyropia aquaculture rafts to green tides in the Yellow Sea显示文摘Green tides caused by the unusual accumulation of high floating Ulva prolifera have occurred regularly in the Yellow Sea since 2007.The primary source of the Yellow Sea green tides is the attached algae on the Pyropia aquaculture rafts in the Subei Shoal.Ulva prolifera and Blidingia(Italic)sp.are the main species observed on Pyropia aquaculture rafts in the Subei Shoal.We found that U.prolifera has strong buoyancy and a rapid growth rate,which may explain why it is the dominant species of green tides that occur in the China's sea area of the Yellow Sea.The growth rate of floating U.prolifera was about 20%–31%d–1,which was much higher than Blidingia(Italic)sp.There were about 1.7×104 t of attached algae on the Pyropia aquaculture rafts in May 2012.We found that 39%of attached algae could float when the tide rose in the Subei Shoal,and U.prolifera accounted for 63%of the floating algae.Our analysis estimated that about 4000 t of attached U.prolifera floated into the surrounding waters of the Subei Shoal during the recycling period of aquaculture rafts.These results suggest that the initial floating biomass of large-scale green tides in the Yellow Sea is determined by the U.prolifera biomass attached to Pyropia aquaculture rafts,further impacting the scale of the green tide。 | Hongbin Han Shiliang Fan Wei Song Yan Li Jie Xiao Zongling Wang Xuelei Zhang Dewen Ding | 2020 | Acta Oceanologica Sinica2020，39，2： | 3 |
| 13 | Abnormally High Expression of BAFF on T Lymphocytes from Lung Cancer- associated Pleural Effusions and Its Potent Anti-tumor Effect显示文摘在现在的学习,激活从恶意的肋膜的流出( MPE )在 T 淋巴细胞上属于肿瘤坏死因素家庭( BAFF )和它的受体( BAFF-R 和 TACI )的因素的 B 房间的表情被排序的激活荧光的房间( FACS )检验分析,并且与那些相比在从非恶意的肋膜的流出( NMPE )和健康控制的 T 淋巴细胞上。它被发现那 CD3 积极 T 淋巴细胞(包括 CD25 和 CD69 积极房间的 CD4 , CD8 ,和部分)在肺的 MPE ,癌症高度并且一致地表示了 BAFF 分子,当 BAFF 的高表情能仅仅在植物凝集素(公众房产管理局)或白介素被发现时,( IL-2 ) 2 从 NMPE 或健康控制导致了 T 淋巴细胞。这些结果与从由即时 PCR 的 BAFF mRNA 察觉的结果一致。另外,当 TACI 的表达式在 CD4^+ T 房间上被增加,但是减少时,从 MPE 的 T 淋巴细胞显著地从 NMPE 或健康控制比那些表示了更多的 BAFF-R,在 CD8^+ T 房间上什么时候与控制相比。Annexin/PI 试金建议 recombinant 人 BAFF (rhBAFF ) 能从 MPE 支持 T 淋巴细胞的幸存率,当圈套受体 TACI-Fc 熔化蛋白质能支持 T 淋巴细胞的 apoptosis 率时。在 ELISA 试金检测的上层清液的 Cytokines 显示出那 rhBAFF 能显著地起来在像的 TACI-Fc-treated 组在 vitro,和 IFN-gamma 水平调整 IFN-gamma 的分泌物控制组的。所有这些结果显示从 MPE 的 T 淋巴细胞上的 BAFF 的反常地高的表示可以起反肿瘤效果的一个作用。 | Haiyan XU Xiaozhou HE Yibei ZHU Tiangzan YAN Hongbin MA Xueguang ZHANG | 2007 | Acta Biochimica et Biophysica Sinica2007，39，12： | 3 |
| 14 | Surveillance of emerging infectious diseases for biosecurity显示文摘Emerging infectious diseases, such as COVID-19, continue to pose significant threats to human beings and their surroundings. In addition, biological warfare, bioterrorism, biological accidents, and harmful consequences arising from dual-use biotechnology also pose a challenge for global biosecurity. Improving the early surveillance capabilities is necessary for building a common biosecurity shield for the global community of health for all. Furthermore, surveillance could provide early warning and situational awareness of biosecurity risks. However, current surveillance systems face enormous challenges, including technical shortages, fragmented management, and limited international cooperation. Detecting emerging biological risks caused by unknown or novel pathogens is of particular concern. Surveillance systems must be enhanced to effectively mitigate biosecurity risks. Thus, a global strategy of meaningful cooperation based on efficient integration of surveillance at all levels, including interdisciplinary integration of techniques and interdepartmental integration for effective management, is urgently needed. In this paper, we review the biosecurity risks by analyzing potential factors at all levels globally. In addition to describing biosecurity risks and their impact on global security, we also focus on analyzing the challenges to traditional surveillance and propose suggestions on how to integrate current technologies and resources to conduct effective global surveillance. | Rongzhang Hao Yuqi Liu Wanzhu Shen Rongtao Zhao Bo Jiang Hongbin Song Muyang Yan Hui Ma | 2022 | Science China(Life Sciences)2022，65，8： | 2 |
| 15 | Effects of ethanol extracts of scorpion on hippocampal apoptosis and caspase-3 expression in lithium chloride-pilocarpine-induced status epilepticus rats显示文摘BACKGROUND: Previous studies have demonstrated that scorpion venom in the scorpion can inhibit epilepsy and apoptosis.However,it remains unclear whether ethanol extracts of scorpion (EES) exhibit similar effects.OBJECTIVE: To investigate the effects of EES on hippocampal apoptosis and caspase-3 expression,and to compare the effects on sodium valproate (positive control drug) in a rat model of status epilepticus induced by lithium chloride-pilocarpine.DESIGN,TIME AND SETTING: This randomized,controlled study was conducted at the Drug Research and Development Center,Kanghong Pharmaceuticals Group,and the Department of Pathology,Sichuan Academy of Medical Sciences & Sichuan Provincial People’s Hospital,China from May 2007 to April 2008.MATERIALS: EES were prepared by Huashen Pharmaceutical,China.Sodium valproate (Hunan Xiangzhong Pharmaceutical,China) and lithium chloride-pilocarpine (Sigma,USA) were also used in the present study.METHODS: From a total of 156 rats,six served as normal controls.The remaining rats were intraperitoneally injected with lithium chloride-pilocarpine to establish status epilepticus models,and then assigned to five groups (n = 30,respectively).Animals in each group were administered drugs at 15 minutes after epileptic seizure by gavage.i.e.in the normal control and model groups,rats were treated with 1 mL/0.1 kg saline.The sodium valproate group was administered 120 mg/kg/d sodium valproate.The low-,moderate-,and high-dose EES groups received treatments of 290,580,and 1 160 mg/kg/d EES.The dispensed concentration was 1 mL/0.1 kg.Rat seizure behavior was observed.If status epilepticus did not terminated after 1 hour,the rats were intraperitoneally administered atropine (1 mg/kg) and diazepam (10 mg/kg) to terminate seizure.These rats were continuously observed for 6 hours to ensure seizure termination.Then rats were treated with the above-mentioned drugs at 8: 00 am each day until sacrifice,which took place 4 hours after drug administration.MAIN OUTCOME MEASURES: Terminal dUTP nick end labeling (TUNEL)-positive cells and caspase-3 expression were,respectively,determined by TUNEL and immunohistochemistry at 6,24,48,and 72 hours,as well as 7 days,after status epilepticus.Behavioral changes were also measured.RESULTS: A few caspase-3-positive cells were observed.TUNEL-and caspase-3-positive cells were mainly visible in the hippocampal CA1 and CA3 regions 6 hours following status epilepticus in the model and drug intervention groups.The number of TUNEL-positive cells reached a peak at 48 hours following status epilepticus in the sodium valproate group,as well as the moderate-and high-dose EES groups,and number of TUNEL-positive cells reached a peak at 72 hours in the model and low-dose EES groups.The number of caspase-3-positive cells reached a peak at 48 hours in each group.Following treatment of sodium valproate and EES,the number of TUNEL-and caspase-3-positive cells significantly decreased compared with the model group at various time points (P < 0.05).The number of TUNEL-and caspase-3-positive cells was greatest in the low-dose EES group,followed by the moderate-and high-dose EES groups.The number of TUNEL-and caspase-3-positive cells was similar between the sodium valproate and high-dose EES groups.Epileptic seizure was significantly improved in the sodium valproate group,as well as the moderate-and high-dose EES groups,compared with the model group (P < 0.05 or P < 0.01).Treatment with sodium valproate and high-dose EES resulted in the best outcome,although the results were similar (P > 0.05).CONCLUSION: A dose of 1 160 mg/kg/d EES significantly inhibited status epilepticus.This outcome corresponded to a decreased number of apoptotic cells and caspase-3-positive cells,which was similar to sodium valproate.These results suggest that it is not necessary to extract a component from the scorpion for the treatment of epilepsy.The high dose of EES significantly inhibited epilepsy,which correlated with decreased hippocampal caspase-3 expression. | Liang Yu Hongbin Sun Yi Liang Yan Xie Baoming He Fei Xu | 2010 | Neural Regeneration Research2010，5，2： | 2 |
| 16 | Discovery and identification of EIF2AK2 as a direct key target of berberine for anti-inflammatory effects显示文摘Using chemoproteomic techniques,we first identified EIF2AK2,eEF1A1,PRDX3 and VPS4B as direct targets of berberine(BBR)for its synergistically anti-inflammatory effects.Of them,BBR has the strongest affinity with EIF2AK2 via two ionic bonds,and regulates several key inflammatory pathways through EIF2AK2,indicating the dominant role of EIF2AK2.Also,BBR could subtly inhibit the dimerization of EIF2AK2,rather than its enzyme activity,to selectively modulate its downstream pathways including JNK,NF-κB,AKT and NLRP3,with an advantage of good safety profile.In EIF2AK2 gene knockdown mice,the inhibitory IL-1β,IL-6,IL-18 and TNF-a secretion of BBR was obviously attenuated,confirming an EIF2AK2-dependent anti-inflammatory efficacy.The results highlight the BBR's network mechanism on anti-inflammatory effects in which EIF2AK2 is a key target,and inhibition of EIF2AK2 dimerization has a potential to be a therapeutic strategy against inflammationrelated disorders. | Wei Wei Qingxuan Zeng Yan Wang Xixi Guo Tianyun Fan Yinghong Li Hongbin Deng Liping Zhao Xintong Zhang Yonghua Liu Yulong Shi Jingyang Zhu Xican Ma Yanxiang Wang Jiandong Jiang Danqing Song | 2023 | Acta Pharmaceutica Sinica B2023，13，5： | 2 |
| 17 | Scorpion ethanol extract and valproic acid effects on hippocampal glial fibrillary acidic protein expression in a rat model of chronic-kindling epilepsy induced by lithium chloride-pilocarpine显示文摘The present study analyzed the effects of ethanol extracts of scorpion on epilepsy prevention and hippocampal expression of glial fibrillary acidic protein in a lithium chloride-pilocarpine epileptic rat model. Results were subsequently compared with valproic acid. Results showed gradually- increased hippocampal glial fibrillary acidic protein expression following model establishment; glial fibrillary acidic protein mRNA expression was significantly increased at 3 days, reached a peak at 7 days, and then gradually decreased thereafter. Ethanol extracts of scorpion doses of 580 and 1 160 mg/kg, as well as 120 mg/kg valproic acid, led to a decreased number of glial fibrillary acidic protein-positive cells and glial fibrillary acidic protein mRNA expression, as well as decreased seizure grades and frequency of spontaneously recurrent seizures. The effects of 1 160 mg/kg ethanol extracts of scorpion were equal to those of 120 mg/kg valproic acid. These results suggested that the anti-epileptic effect of ethanol extracts of scorpion were associated with decreased hippocampal glial fibrillary acidic protein expression in a rat model of lithium chloride-pilocarpine induced epilepsy. | Yi Liang Hongbin Sun Liang Yu Baoming He Yan Xie | 2012 | Neural Regeneration Research2012，7，6： | 2 |
| 18 | Optimal time for subarachnoid transplantation of neural progenitor cells in the treatment of contusive spinal cord injury显示文摘This study aimed to identify the optimal neural progenitor cell transplantation time for spinal cord injury in rats via the subarachnoid space. Cultured neural progenitor cells from 14-day embryonic rats, constitutively expressing enhanced green fluorescence protein, or media alone, were injected into the subarachnoid space of adult rats at 1 hour (acute stage), 7 days (subacute stage) and 28 days (chronic stage) after contusive spinal cord injury. Results showed that grafted neural progenitor cells migrated and aggregated around the blood vessels of the injured region, and infiltrated the spinal cord parenchyma along the tissue spaces in the acute stage transplantation group. However, this was not observed in subacute and chronic stage transplantation groups. O4- and glial fibrillary acidic protein-positive cells, representing oligodendrocytes and astrocytes respectively, were detected in the core of the grafted cluster attached to the cauda equina pia surface in the chronic stage transplantation group 8 weeks after transplantation. Both acute and subacute stage transplantation groups were negative for O4 and glial fibrillary acidic protein cells. Basso, Beattie and Bresnahan scale score comparisons indicated that rat hind limb locomotor activity showed better recovery after acute stage transplantation than after subacute and chronic transplantation. Our experimental findings suggest that the subarachnoid route could be useful for transplantation of neural progenitor cells at the acute stage of spinal cord injury. Although grafted cells survived only for a short time and did not differentiate into astrocytes or neurons, they were able to reach the parenchyma of the injured spinal cord and improve neurological function in rats. Transplantation efficacy was enhanced at the acute stage in comparison with subacute and chronic stages. | Yan Liu Ying Zhou Chunli Zhang Feng Zhang Shuxun Hou Hongbin Zhong Hongyun Huang | 2013 | Neural Regeneration Research2013，8，5： | 2 |
| 19 | P2X4 receptor and brain-derived neurotrophic factor in neuropathic pain显示文摘Objective:To investigate whether the activation of p38MAPK is involved in the neuropathic pain induced by P2X4 receptor,and the effects of activated P2X4 receptor and p38MAPK on expression of brain-derived neurotrophic factor (BDNF) in the chronic neuropathic pain.Methods:Lumbar intrathecal catheters were chronically implanted in male Sprague-Dawley rats.The right sciatic nerve was loosely ligated proximal to the sciatica's trifurcation at approximately 1.0 mm intervals with 4-0 silk sutures.The microglia inhibitor minocycline,P2X4 antagonist (TNP-ATP) and p38MAPK inhibitor (SB203580) were intrathecally administered every 12 h,3 d post-chronic constriction injury (CCI).Mechanical nociceptive thresholds were assessed with the paw withdrawal threshold (PWT) to von Frey filaments.The expression of P2X4 and BDNF were assessed by both immunohistochemical analysis and RT-PCR.Results:Intrathecal injection of minocycline or TNP-ATP or SB203580 significantly attenuated CCI-induced mechanical allodynia.The time courses of P2X4 receptor and BDNF expression were increased at all points after CCI and reached a peak level on postoperative d 7.Intrathecal injection of minocycline or TNP-ATP or SB203580 markedly suppressed the increase of CCI-induced P2X4 receptor and BDNF expression in the spinal cord.Conclusion:The activation of P2X4 receptor BDNF pathways contributes to neuropathic pain in CCI rats,and the activation of p38MAPK is involved in the neuropathic pain induced by P2X4 receptor. | Yan Xiaodi Zhou Shuangqiong Chen Qianbo Chen Chengwen Yuan Hongbin | 2010 | Journal of Medical Colleges of PLA(China)2010，25，5： | 2 |
| 20 | EXPERIMENTAL AND THEORETICAL STUDY OF STATIC-DYNAMIC MIXED MODE BRITTLE FRACTURE OF ROCK显示文摘Variousmode-Ⅰinvestigationsinthetheoryoflinearelasticfracturemechanicsareimportantindeterminingthefractureconstants.Toanalyse... | Li Zhenzi Lin Guoxiang Xu Jicheng Zhang Jingyi Luo Zhang (Department of Architecture Engineering, Central South University of Technology,Changsha 410083,China) Zhou Hongbin Li Huijian (Department of Civil Engineering,Yan shan University,H | 1998 | Journal of Central South University1998，10，2： | 2 |
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