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| 1 | Metabolic engineering and flux analysis of Corynebacterium glutamicum for L-serine production显示文摘L-Serine plays a critical role as a building block for cell growth, and thus it is difficult to achieve the direct fermentation of L-serine from glucose. In this study, Corynebacterium glutamicum ATCC 13032 was engineered de novo by blocking and attenuating the conversion of L-serine to pyruvate and glycine, releasing the feedback inhibition by L-serine to 3-phosphoglycerate dehydrogenase (PGDH), in combination with the co-expression of 3-phosphoglycerate kinase (PGK) and feedback-resistant PGDH (PGDHr). The resulting strain, SER-8, exhibited a lower specific growth rate and significant differences in L-serine levels from Phase I to Phase V as determined for fed-batch fermentation. The intracellular L-serine pool reached (14.22±1.41) μmol gCDM 1, which was higher than glycine pool, contrary to fermentation with the wild-type strain. Furthermore, metabolic flux analysis demonstrated that the over-expression of PGK directed the flux of the pentose phosphate pathway (PPP) towards the glycolysis pathway (EMP), and the expression of PGDHr improved the L-serine biosynthesis pathway. In addition, the flux from L-serine to glycine dropped by 24%, indicating that the deletion of the activator GlyR resulted in down-regulation of serine hydroxymethyltransferase (SHMT) expression. Taken together, our findings imply that L-serine pool management is fundamental for sustaining the viability of C. glutamicum, and improvement of C1 units generation by introducing the glycine cleavage system (GCV) to degrade the excessive glycine is a promising target for L-serine production in C. glutamicum. | LAI ShuJuan ZHANG Yun LIU ShuWen LIANG Yong SHANG XiuLing CHAI Xin WEN TingYi | 2012 | Science China(Life Sciences)2012，55，4： | 15 |
| 2 | Modeling xeroderma pigmentosum associated neurological pathologies with patients-derived iPSCs显示文摘干皮病 pigmentosum (XP ) 是联系 XP 的基因的变化引起的一组基因混乱,导致 DNA 修理的缺陷。XP 病人经常展出神经病学的退化,而是内在的机制是未知的,部分地由于合适的疾病模型的缺乏。这里,我们产生了包括 XPA, XPB, XPC, XPG,和 XPV 在五不同 XP 基因怀有变化的病人特定的导致的 pluripotent 干细胞(iPSCs ) 。这些 iPSCs 进一步被区分到神经房间,并且他们到 DNA 损坏应力的危险性被调查。在神经干细胞(NSC ) 或神经原的 XPA 的变化导致了严重 DNA 损坏修理缺点,并且有变异的 XPA 的这些神经房间对 DNA 导致损坏的 apoptosis 过分敏感。因此, XP 变异的神经房间代表珍贵工具在 XP 病人澄清神经病学的畸形的分子的机制。 | Lina Fu Xiuling Xu Ruotong Ren Jun Wu Weiqi Zhang Jiping Yang Xiaoqing Ren Si Wang Yang Zhao Liang Sun Yang Yu Zhaoxia Wang Ze Yang Yun Yuan Jie Qiao Juan Carlos Izpisua Belmonte Jing Qu Guang-Hui Liu | 2016 | Protein & Cell2016，7，3： | 11 |
| 3 | CRISPR/Cas9-mediated targeted gene correction in amyotrophic lateral sclerosis patient iPSCs显示文摘 | Lixia Wang Fei Yi Lina Fu Jiping Yang Si Wang Zhaoxia Wang Keiichiro Suzuki Liang Sun Xiuling Xu Yang Yu Jie Qiao Juan Carlos Izpisua Belmonte Ze Yang Yun Yuan Jing Qu Guang-Hui Liu | 2017 | Protein & Cell2017，8，5： | 10 |
| 4 | Bio-geomorphologic features and growth process of Tamarix nabkhas in Hotan River Basin, Xinjiang显示文摘Tamarix nabkha 是最普遍的 nabkhas 之一,在中国的干旱区域散布。在实验室基于户外的观察和模拟实验,在这份报纸的分析在 Hotan 河的中间、更低的活动范围指生物 geomorphologic 特征和 Tamarix nabkhas 的生长过程, Xinjiang。并且结果显示在学习区域的 Tamarix 的生态的类型基于 Populus Diversifolia 森林和灌木的深土壤是一种 Tugaic 土壤产地。这类产地能被划分成在微分发达阶段期间表明 Tamarix nabkhas 的生态的环境的特征的三种亚产地。同时, Tamarix nabkha 能在地面,和它的根和茎上在风沙流动上施加加强的骚乱电流不仅有发芽的强壮的潜力但是风侵蚀忍耐是特征的,被沙并且分别地要埋葬的电阻木质化的分支僵硬的流氓,为它有相当更长的一生。因此, Tamarix nabkha 影响的风速度侧面与 Phragmites nabkha 和 Alhagi nabkha 不同。并且风流动的结构对风神的沙在里面 / 附近 Tamarix 积累有益灌木,它能与更高平均的坡度和生活的更长的频率创造唯一的 Tamarix nabkhas。在区域的 Tamarix nabkha 进化经历了三个阶段:生长舞台,成熟、稳定的阶段和凋谢的舞台。在每个阶段,词法特征和 Tamarix nabkha 的似地球形状的过程由于在 nabkha 和风神的沙流动之间的差异的相互作用是不同的。 | LI Zhizhong WU Shengli CHEN Shujiang CHEN Xiuling JIN Jianhui LIU QI | 2010 | Journal of Geographical Sciences2010，20，2： | 10 |
| 5 | A transgene-free method for rapid and efficient generation of precisely edited pigs without monoclonal selection显示文摘Gene-edited pigs for agricultural and biomedical applications are typically generated using somatic cell nuclear transfer(SCNT).However, SCNT requires the use of monoclonal cells as donors, and the time-consuming and laborious monoclonal selection process limits the production of large populations of gene-edited animals. Here, we developed a rapid and efficient method named RE-DSRNP(reporter RNA enriched dual-sg RNA/CRISPR-Cas9 ribonucleoproteins) for generating gene-edited donor cells. RE-DSRNP takes advantage of the precise and efficient editing features of dual-sg RNA and the high editing efficiency, low off-target effects, transgene-free nature, and low cytotoxic characteristics of reporter RNA enriched RNPs(CRISPR-Cas9ribonucleoproteins), thus eliminating the need for the selection of monoclonal cells and thereby greatly reducing the generation time of donor cells from 3–4 weeks to 1 week, while also reducing the extent of apoptosis and chromosomal aneuploidy of donor cells. We applied RE-DSRNP to produce cloned pigs bearing a deletion edit of the wild-type p53-induced phosphatase 1(WIP1)gene: among 32 weaned cloned pigs, 31(97%) carried WIP1 edits, and 15(47%) were homozygous for the designed fragment deletion, and no off-target event was detected. The WIP1 knockout(KO) pigs exhibited male reproductive disorders, illustrating the utility of RE-DSRNP for rapidly generating precisely edited animals for functional genomics and disease research. REDSRNP's strong editing performance in a large animal and its marked reduction in the required time for producing SCNT donor cells support its application prospects for rapidly generating populations of transgene-free cloned animals. | Kui Xu Xiuling Zhang Zhiguo Liu Jinxue Ruan Changjiang Xu Jingjing Che Ziyao Fan Yulian Mu Kui Li | 2022 | Science China(Life Sciences)2022，65，8： | 9 |
| 6 | Molecular mapping of QTLs for root response to phosphorus deficiency at seedling stage in wheat (Triticum aestivum L.)显示文摘Phosphorus (P) deficiency in the soil is one of the major abiotic stresses that limit plant growth and crop productivity throughout the world. Development of cultivars with improved P-deficiency tolerance is an efficient strategy for sustainable agriculture. Plant roots play an important role in crop growth and development, especially in nutrient uptake and improvement of P-efficiency. Mapping quantitative trait loci (QTLs) for root traits and their response to low P stress at seedling stage will facilitate the development of P-efficient wheat cultivars. In this study, 30 QTLs (LOD>2.0) were mapped for the three root traits, such as root length, root number and root dry matter under different P supply conditions and their response to P-stress. These QTLs were distributed on 14 chromosomes, with each of the 5 QTLs explaining more than 10% phenotype variance. Analyses showed that root traits and their response to P-deficiency were controlled by different QTLs. In addition, alleles with positive effects were separated on both parents, and wheat cultivars with improved P-efficiency could be developed by accumulating these positive effect alleles together. | Li Zhenxing Ni Zhongfu Peng Huiru Liu Zhiyong Nie Xiuling Xu Shengbao Liu Gang Sun Qixin | 2007 | Progress in Natural Science:Materials International2007，17，10： | 8 |
| 7 | Optimization of a new animal glue binder system cured by CO_2 for use in foundry显示文摘A new sand binder system cured by CO2 was prepared based on the animal bone glue.To overcome the disadvantages of animal glue such as agglomeration at room temperature,high energy consumption and low efficiency,an alkaline decomposition process was selected,and certain modifier was used to modify the performance of the animal glue binder.For the alkaline decomposition,NaOH was used as the catalyst with an addition of 4wt.% to the animal glue.A modifier was determined through the orthogonal experiment with a weight ratio of glycerin:glycol:dextrin:animal glue = 9:16:15:100,and the optimal modification reaction should be performed at 75 ℃ with a reaction time of 90 min.Ca(OH)2 was used as a promoter;the optimal CO2 gas flux blowing into the sand was 0.7 m3.h-1 for a duration of 60s under the experimental conditions.Results show that an original strength above 0.7 MPa and a final strength about 4.2 MPa can be achieved,which could meet the requirement of rapid moulding and core-making for foundry.The new binder was characterized and analyzed by means of IR,and the modification and CO2-cured mechanisms of this animal glue binder were also discussed. | Liu Weihua Zhang Yilin Li Yingmin Liu Xiuling | 2012 | China Foundry2012，9，4： | 5 |
| 8 | Introduction of antisense waxy gene into main parent lines of indica hybrid rice显示文摘Amylose content in rice endosperm is one of the key determinants of rice eating and cooking quality, and the poor quality of indica hybrid rice is closely related to the high amylose level in rice grains. In order to improve the grain quality of the indica hybrid rice by genetic engineering, an antisense fragment of rice waxy gene, driven by the 5’-franking sequences of the rice waxy gene, was successfully introduced into three major parent lines of indica hybrid rice, all contain a high amylose level in the grains, via Agrobacte-rium, and more than 100 hygromycin-resistant plants were regenerated. The analysis of PCR amplification and Southern blots indicated that the T-DNA containing the antisense waxy gene had been integrated into the genome of transgenic rice plants. Most of the primary transgenic rice plants grew normally, and the mature seeds from these transgenic plants were performed for analysis of the amylose content. The results showed that the amylose content in the endosperm of some grains | CHEN Xiuhua LIU Qiaoquan WANG Zongyang WANG Xingwen CAI Xiuling ZHANG Jingliu GU Minghong | 2002 | Chinese Science Bulletin2002，47，14： | 5 |
| 9 | A widely adaptable approach to generate integration-free iPSCs from non-invasively acquired human somatic cells显示文摘 | Zhichao Ding Lina Sui Ruotong Ren Yanjun Liu Xiuling Xu Lina Fu Ruijun Bai Tingting Yuan Ying Hao Weiqi Zhang Huize Pan Wensu Liu Han Yu Concepcion Rodriguez Esteban Xiaobing Yu Ze Yang Jian Li Xiaomin Wang Juan Carlos Izpisua Belmonte Guang-Hui Liu Fei Yi Jing Qu | 2015 | Protein & Cell2015，6，5： | 5 |
| 10 | A Hierarchically Porous Metal-Organic Framework from Semirigid Ligand for Gas Adsorption显示文摘层次地多孔的材料在便于集体运输和吸附和分离过程的改善效率起一个重要作用。在这份报纸,新策略被建议认识到一个层次地多孔的金属器官的框架([Cu 2(OH)(L)]·(DMF)0.8(FJU-11, H 3L=3,5-(4-carboxybenzyloxy ) 安息香的酸, DMF=N, N-dimethylformamide ) 经由使用半硬的多羧基的酸。有趣地, FJU-11 向公司 2 拥有大吸附能力和小 isosteric 热。为 FJU-11 的列突破实验在烟道气体的分离加亮它的潜在的申请。 | Lizhen Liu Yingxiang Ye Zizhu Yao Liuqin Zhang Ziyin Li Lihua Wang Xiuling Ma Qian-Huo Chen Zhangjing Zhang Shengchang Xiang | 2016 | Chinese Journal of Chemistry2016，34，2： | 4 |
| 11 | Coal petrology and genesis of Jurassic coal in the Ordos Basin,China显示文摘简单结构和浅埋葬深度描绘的厚煤床的多重集合在 Ordos 盆的早、中间的侏罗记阶层被开发,西北的中国。这高质量的煤的巨大的储备有高商业的价值。我们学习了煤岩石学的特征和它的 maceral 分发到决定 macerals 贡献到油和气体的产生。结果证明因为主要是在上面的河的系统的一系列高看台的沼泽地的盆唯一的 depositional 环境,在 Ordos 盆的侏罗记煤有特殊 petrological 特征。这些 petrographic 特征是一些沙身体被移植河形成的典型内陆湖的发展的结果。在埋葬以后,泥炭继续经历氧化条件,产生广泛的更高的 inertinite 在煤满足的这个过程和 vitrinite 部件被改变到 semi-vitrinite。这些侏罗记煤的宏观的 petrographic 类型主要是半迟钝的煤,迟钝的煤, semilustrous 和有光泽的煤。半迟钝的煤和迟钝的煤的比例在盆边缘是更高的,特别在北边缘附近的区域。semilustrous 和有光泽的煤的数字向南并且向中央盆增加。这种状况显示不同形成煤的沼泽地环境让学生在煤部件上控制效果。另一观察是在 Ordos 煤序列,特别在更低的部分,一些沙岩床是厚的,直到有一种粗糙的谷物尺寸的 20 m。在 macerals 的更高的 fusinite 内容与更多的完全、常规的植物房间结构伴随一个更高的 semi-vitrinite 内容。fusinite 结构清楚、很好保存。在埋葬以后,房顶和地板岩石的岩性学能继续影响煤岩石学的进化。在房顶和地板的沙身体展出好物理条件以便毛孔水能维持氧化,循环和连接的一个长期的状态到煤。煤部件因此很长时间留在氧化环境。在盆中心,在埋葬和随后的煤床很快进入了减少的环境以后,相反地,开发的湖的外形和泥炭被 mudstone 很快盖住。作为结果,丰富的胶凝化作用发生了, vitrinite 内容增加了。Exinite 经常在煤床上在一个特定的位置积累了。尽管平均 exinite 内容不总体上高,它显著地确实贡献全部的烃产生。exinite 内容被低估了,特别非结晶的沥青的液体和它的重要性这里被强调。原因是液体容易流进有强壮的刚硬的 fusinite,或流进一些裂缝,在它通常被忽视的地方。 | Weihua Ao Wenhui Huang Chengmin Weng Xiuling Xiao Dameng Liu Xiuyi Tang Ping Chen Zhigen Zhao Huan Wan Robert B.Finkelman | 2012 | Geoscience Frontiers2012，3，1： | 3 |
| 12 | Variation of the Vaginal Microbiome During and After Pregnancy in Chinese Women显示文摘A comprehensive profiling of the vaginal microbial communities and their variability enables an accurate description of the microbiome in women.However,there is a lack of studies available on Chinese women.In the present study,the composition of the vaginal microbiota during pregnancy and the 6-week postpartum period of 454 Chinese women was characterized by sequencing the V3-V4 region of the 16S ribosomal RNA(rRNA)gene.The vaginal microbiome showed variations during pregnancy and the postpartum period based on the abortion history,hypertensive disorders,delivery mode,and maternal age.Co-variation of 22 bacterial taxa,including the Lactobacillus genus and two of its species,may account for the common characteristics of the vaginal microbiome under scenarios of different medical histories and pregnancy outcomes.In contrast,discriminant bacterial species were significantly different between women who had preterm birth(PTB)with and without premature rupture of membranes(PROM),and the community state type(CST)IV-A without any predominant Lactobacillus species in the microbiota was more prevalent during pregnancy in the PROM-PTB cases,suggesting that specific bacterial species could be considered to distinguish between different types of PTB.By providing data on Chinese women,this study will enrich the knowledge of the human microbiome and contribute to a better understanding of the association between the vaginal microbiome and reproductive health. | Xiaoai Zhang Qingzhi Zhai Jinfeng Wang Xiuling Ma Bo Xing Hang Fan Zhiying Gao Fangqing Zhao Wei Liu | 2022 | Genomics, Proteomics & Bioinformatics2022，20，2： | 2 |
| 13 | Omentin-1 is associated with carotid atherosclerosis in patients with metabolic syndrome显示文摘 | Rong Liu Xiuling Wang Peili Bu | 2011 | Diabetes Research and Clinical Practice2011，，1： | 2 |
| 14 | Antibiotic resistance gene abundances associated with antibiotics and heavy metals in animal manures and agricultural soils adjacent to feedlots in Shanghai; China显示文摘 | Xiuling Ji Qunhui Shen Fang Liu Jing Ma Gang Xu Yuanlong Wang Minghong Wu | 2012 | Journal of Hazardous Materials2012，，： | 2 |
| 15 | Converted neural cells: induced to a cure?显示文摘Many neurodegenerative disorders such as Parkinson’s disease(PD),amyotrophic lateral sclerosis(ALS)and others often occur as a result of progressive loss of structure or function of neurons.Recently,many groups were able to generate neural cells,either differentiated from induced pluripotent stem cells(iPSCs)or converted from somatic cells.Advances in converted neural cells have opened a new era to ease applications for modeling diseases and screening drugs.In addition,the converted neural cells also hold the promise for cell replacement therapy(Kikuchi et al.,2011;Krencik et al.,2011;Kriks et al.,2011;Nori et al.,2011;Rhee et al.,2011;Schwartz et al.,2012).Here we will mainly discuss most recent progress on using converted functional neural cells to treat neurological diseases and highlight potential clinical challenges and future perspectives. | Weiqi Zhang Shunlei Duan Ying Li Xiuling Xu Jing Qu Weizhou Zhang Guang-Hui Liu | 2012 | Protein & Cell2012，3，2： | 2 |
| 16 | The Design and Dynamic Analysis of a Novel 6--DOF Parallel Mechanism 显示文摘 | Liu Xiuling Wang Qingquan Malikov A | 2012 | International Journal of Machine Learning and Cybernetics2012，3，1： | 1 |
| 17 | Direct Solution Synthesis of Corundum-type In203: Effects of Precursors on Products显示文摘 | LIU Guodong ChEN Dairong JIAO Xiuling | 2009 | CrystEngComm2009，11，： | 1 |
| 18 | Antibiotic resistance gene abundances associated with antibiotics and heavy metals in animal manures and agricultural soils adjacent to feedlots in Shanghai; China显示文摘 | Xiuling Ji Qunhui Shen Fang Liu Jing Ma Gang Xu Yuanlong Wang Minghong Wu | 2012 | Journal of Hazardous Materials2012，，： | 1 |
| 19 | A Spatio-temporal Data Model for Road Network in Data Center Based on Incremental Updating in Vehicle Navigation System显示文摘The technique of incremental updating,which can better guarantee the real-time situation of navigational map,is the developing orientation of navigational road network updating.The data center of vehicle navigation system is in charge of storing incremental data,and the spatio-temporal data model for storing incremental data does affect the efficiency of the response of the data center to the requirements of incremental data from the vehicle terminal.According to the analysis on the shortcomings of several typical spatio-temporal data models used in the data center and based on the base map with overlay model,the reverse map with overlay model (RMOM) was put forward for the data center to make rapid response to incremental data request.RMOM supports the data center to store not only the current complete road network data,but also the overlays of incremental data from the time when each road network changed to the current moment.Moreover,the storage mechanism and index structure of the incremental data were designed,and the implementation algorithm of RMOM was developed.Taking navigational road network in Guangzhou City as an example,the simulation test was conducted to validate the efficiency of RMOM.Results show that the navigation database in the data center can response to the requirements of incremental data by only one query with RMOM,and costs less time.Compared with the base map with overlay model,the data center does not need to temporarily overlay incremental data with RMOM,so time-consuming of response is significantly reduced.RMOM greatly improves the efficiency of response and provides strong support for the real-time situation of navigational road network. | WU Huisheng LIU Zhaoli ZHANG Shuwen ZUO Xiuling | 2011 | Chinese Geographical Science2011，21，3： | 1 |
| 20 | Transfer matrix method of flatness control for strip mills显示文摘 | LIU Hongmin ZHANG Xiuling WANG Yingrui | 2005 | Journal of Materials Processing Technology2005，166，2： | 1 |
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