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| 1 | Overexpression of IbPAL1 promotes chlorogenic acid biosynthesis in sweetpotato显示文摘Sweetpotato[Ipomoea batatas(L.)Lam.],a food crop with both nutritional and medicinal uses,plays essential roles in food security and health-promoting.Chlorogenic acid(CGA),a polyphenol displaying several bioactivities,is distributed in all edible parts of sweetpotato.However,little is known about the specific metabolism of CGA in sweetpotato.In this study,IbPAL1,which encodes an endoplasmic reticulum-localized phenylalanine ammonia lyase(PAL),was isolated and characterized in sweetpotato.CGA accumulation was positively associated with the expression pattern of IbPAL1 in a tissue-specific manner,as further demonstrated by overexpression of IbPAL1.Overexpression of IbPAL1 promoted CGA accumulation and biosynthetic pathway genes expression in leaves,stimulated secondary xylem cell expansion in stems,and inhibited storage root formation.Our results support a potential role for IbPAL1 in sweetpotato CGA biosynthesis and establish a theoretical foundation for detailed mechanism research and nutrient improvement in sweetpotato breeding programs. | Yang Yu Yingjie Wang Yue Yu Peiyong Ma Zhaodong Jia Xiaoding Guo Yizhi Xie Xiaofeng Bian | 2021 | The Crop Journal2021，9，1： | 5 |
| 2 | An AIEgens and exonuclease Ⅲ aided quadratic amplification assay for detecting and cellular imaging of telomerase activity显示文摘Monitoring telomerase activity with high sensitive and reliable is of great importance to cancer analysis.In this paper, we report a sensitive and facile method to detect telomerase activity using AIEgens modified probe(TPE-Py-DNA) as a fluorescence reporter and exonuclease Ⅲ(Exo Ⅲ) as a signal amplifier.With the aid of telomerase, repeat units(TTAGGG)n are extended from the end of template substrate oligonucleotides(TS primer) that form duplex DNAs with TPE-Py-DNA. Then, Exo Ⅲ catalyzes the digestion of duplex DNAs, liberating elongation product and releasing hydrophobic TPE-Py. The released hydrophobic TPE-Py aggregate together and produce a telomerase-activity-related fluorescence signal.The liberated product hybridizes with another TPE-Py-DNA probe, starting the second cycle. Finally,we obtain the target-to-signal amplification ratio of 1:N^2. This strategy exhibits good performance for detecting clinical urine samples(distinguishing 15 cancer patients' samples from 8 healthy ones) and checking intracellular telomerase activity(differentiating cell lines including He La, MDA-MB-231,MCF-7, A375, HLF and MRC-5 from the cells pretreated with telomerase-related drug), which shows its potential in clinical diagnosis as well as therapeutic monitoring of cancer. | Xuehong Min Lei Xia Yuan Zhuang Xudong Wang Jie Du Xiaojin Zhang Xiaoding Lou Fan Xia | 2017 | Science Bulletin2017，62，14： | 5 |
| 3 | Spatiotemporal variations of nitrate sources and dynamics in a typical agricultural riverine system under monsoon climate显示文摘Nitrogen pollution is a serious environmental issue in the Danjiangkou Reservoir region(DRR),the water source of the South-to-North Water Diversion Project of China.In this research,seasonal surveys and a bi-weekly time series survey were conducted in the Qihe River Basin,one of the most densely populated agricultural basins in the DRR.Hydrochemical compositions(NO3^-Cl^-),dual isotopes(δD-H2O,δ^18O-H2O,δ^15N-NO3^-,andδ^18O-NO3^-),and a Markov Chain Monte Carlo isotope mixing model were jointly applied to unravel the sources,migrations,and transformations of the nitrate(NO3^-)in the basin.It was revealed that the mixing between different sources was the main process controlling the isotopic compositions of the riverine NO3the upper-middle reaches.In contrast,denitrification occurred in the lower reaches.For the first time,the sources of NO3^-quantified at a basin scale in the DRR.Overall,the river transported 484.2 tons/year of NO3^-N to the reservoir,of which 32.6%,36.4%,28.0%,and 3.0%was from soil organic nitrogen,chemical fertilizer,residential sewage and atmospheric precipitation,respectively.The NO3^-N fluxes of the different sources were regulated by the monsoon climate and anthropogenic activities.For example,high precipitation and intense fertilization resulted in severe nonpoint source pollution.Denitrification thrived in soils and reservoirs in wet seasons.Temperature could regulate the migration,nitrification and denitrification processes.Based on the results,we suggest that the management strategies dealing with nitrogen pollution issue in the DRR should follow the specific spatiotemporal characteristics of NO3migration and transformation mechanisms. | Hao Jiang Wenjing Liu Jiangyi Zhang Li Zhou Xiaode Zhou Ke Pan Tong Zhao Yuchen Wang Zhifang Xu | 2020 | Journal of Environmental Sciences2020，32，7： | 5 |
| 4 | Statistic characteristics of severe convective storm during Warm-Season in the Beijing-Tianjin region and its vicinity显示文摘This study analyzed the climatological characteristics of severe convective storms in the Beijing and Tianjin region and its vicinity based on the Doppler radar data of Tanggu during May―August of 2003― 2007. The climatological characteristics, e.g. storm area, volume, top height, max reflectivity, life time and motion, are analyzed. The results include: 75% of all storms in the Beijing-Tianjin region last no more than 30 minutes, and most storms have a volume less than 400 km3; most storms move from southwest to northeast while the speed is between 10―30 km/h; the mean storm top height is about 6 km, but some strong convective storms can have a top height larger than 15 km; finally, storm area and volume have a similar geographical distribution character showing increasing trends from west to east. Compared with the statistic results based on the conventional surface meteorological observations, the results based on the radar data can present not only 3D spatial statistic results of convective storms (e.g., volume and top height), but also the quantitative climatological characteristics, such as the life-time and speed distributions. These statistical results are useful for studying the climatic characteristics of convective storms in the Beijing-Tianjin region and its vicinity. | HAN Lei YU XiaoDing ZHENG YongGuang CHEN MingXuan WANG HongQing LIN YinJing | 2009 | Chinese Science Bulletin2009，54，14： | 5 |
| 5 | NeuroBiometric:An Eye Blink Based Biometric Authentication System Using an Event-Based Neuromorphic Vision Sensor显示文摘The rise of the Internet and identity authentication systems has brought convenience to people's lives but has also introduced the potential risk of privacy leaks.Existing biometric authentication systems based on explicit and static features bear the risk of being attacked by mimicked data.This work proposes a highly efficient biometric authentication system based on transient eye blink signals that are precisely captured by a neuromorphic vision sensor with microsecond-level temporal resolution.The neuromorphic vision sensor only transmits the local pixel-level changes induced by the eye blinks when they occur,which leads to advantageous characteristics such as an ultra-low latency response.We first propose a set of effective biometric features describing the motion,speed,energy and frequency signal of eye blinks based on the microsecond temporal resolution of event densities.We then train the ensemble model and non-ensemble model with our Neuro Biometric dataset for biometrics authentication.The experiments show that our system is able to identify and verify the subjects with the ensemble model at an accuracy of 0.948 and with the non-ensemble model at an accuracy of 0.925.The low false positive rates(about 0.002)and the highly dynamic features are not only hard to reproduce but also avoid recording visible characteristics of a user's appearance.The proposed system sheds light on a new path towards safer authentication using neuromorphic vision sensors. | Guang Chen Fa Wang Xiaoding Yuan Zhijun Li Zichen Liang Alois Knoll | 2021 | IEEE/CAA Journal of Automatica Sinica2021，8，1： | 3 |
| 6 | Genomic footprints of Kam Sweet Rice domestication indicate possible migration routes of theDongpeople inChina andprovide resources for future rice breeding显示文摘The Dong people are one of China’s 55 recognized ethnic minorities,but there has been a long-standing debate about their origins.In this study,we performed whole-genome resequencing of Kam Sweet Rice(KSR),a valuable,rare,and ancient rice landrace unique to the Dong people.Through comparative genomic analyses of KSR and other rice landraces from south of the Yangtze River Basin in China,we provide evidence that the ancestors of the Dong people likely originated from the southeast coast of China at least 1000 years ago.Alien introgression and admixture in KSR demonstrated multiple migration events in the history of the Dong people.Genomic footprints of domestication demonstrated characteristics of KSR that arose from artificial selection and geographical adaptation by the Dong people.The key genes GS3,Hd1,and DPS1(related to agronomic traits)and LTG1 and MYBS3(related to cold tolerance)were identified as domestication targets,reflecting crop improvement and changes in the geographical environment of the Dong people during migration.A genome-wide association study revealed a candidate yield-associated gene,Os01g0923300,a specific haplotype in KSR that is important for regulating grain number per panicle.RNA-sequencing and quantitative reverse transcription-PCR results showed that this gene was more highly expressed in KSR than in ancestral populations,indicating that it may have great value in increasing yield potential in other rice accessions.In summary,our work develops a novel approach for studying human civilization and migration patterns and provides valuable genomic datasets and resources for future breeding of high-yield and climate-resilient rice varieties. | Chunhui Liu Tianyi Wang Huicha Chen Xiaoding Ma Chengzhi Jiao Di Cui Bing Han Xiaobing Li Aixia Jiao Renchao Ruan Dayuan Xue Yanjie Wang Longzhi Han | 2023 | Molecular Plant2023，16，2： | 3 |
| 7 | The effects of different nitrogen sources on camptothecin content and related gene expression in Camptotheca acuminata seedlings显示文摘Camptotheac acuminata Decne is a unique tree species in China with an important secondary metabolite,camptothecin(CPT),used in the treatment of cancer.Nitrogen(N)is an important element that affects plant growth and the accumulation of CPT.Reports on the effect of N on CPT synthesis from a genetic perspective are scarce.To explore the effects of different N sources and levels on CPT synthesis in C.acuminata,two-year-old seedlings were fertilized with different concentrations of pure ammonium sulphate,source of ammonium N(NH4+-N),and potassium nitrate for nitrate N(NO3--N).Concentrations of 2.5,5,7.5,and10 g pot-1 NH4+-N and NO3--N were used.The results showed that 7.5 g NH4+-N and NO3--N treatments were best for growth and fresh weight of leaves.Compared with the other treatments,the CPT content,tryptophan synthase and tryptophan decarboxylase activities,and expression of the CaTSB and CaTDCl genes under the 2.5 g NH4+-N and NO3--N treatments peaked significantly at 30 days.However,the expression of CaTDC2 surpassed that of the other two genes at 60 days.Therefore,compared with NH4+-N source,the NO3--N source was more beneficial for growth,and NO3--N was better for CPT yield.Consequently,leaves of C.acuminata treated with 2.5 g NO3--N could be harvested after 30 days to obtain maximum CPT content.CaTDC1 is more closely linked to CPT synthesis.The results of this study improved the production of CPT in C.acuminata via fertilization. | Xiaode Wang Sainan Bian Pengjie Chang Ninghang Wang Lingjuan Xuan Mingru Zhang Bin Dong Chao Zhang Jiasheng Wu Yeqing Ying Xiazhen Lin Yamei Shen | 2020 | Journal of Forestry Research2020，31，4： | 3 |
| 8 | Electrodynamic Tethered Deorbit System Dynamics and Performance Analysis显示文摘Electrodynamic tethered deorbit technology is a novel way to remove abandoned spacecrafts like upper stages or unusable satellites. This paper investigates and analyses the deorbit performance and mission applicability of the electrodynamic tethered system. To do so, the electrodynamic tethered deorbit dynamics with multi-perturbation is firstly formulated, where the Earth magnetic field, the atmospheric drag, and the Earth oblateness effect are considered. Then, the key system parameters, including payload mass, tether length and tether type, are analyzed by numerical simulations to investigate their influences on the deorbit performance and to give the setting principles for choosing system parameters. Based on this and given an appropriate group of system parameters, numerical simulations are undertaken to study the impact of the mission parameters, including orbit height and orbit inclination, and thus to investigate the mission applicability of the electrodynamic tethered deorbit technology. | ZHANG Feng SHEN Lin WU Shengbao WANG Xiaoding WANG Shuting YU Bensong | 2016 | Aerospace China2016，17，3： | 2 |
| 9 | In situ formation of thermosensitive P (NIPAAm-co-GMA)/PEI hydrogels显示文摘 | Wang Zongchun Xu Xiaoding | | 0，，： | 1 |
| 10 | An intelligent and privacy-enhanced data sharing strategy for blockchain-empowered Internet of Things显示文摘With the development of the Internet of Things(IoT),the massive data sharing between IoT devices improves the Quality of Service(QoS)and user experience in various IoT applications.However,data sharing may cause serious privacy leakages to data providers.To address this problem,in this study,data sharing is realized through model sharing,based on which a secure data sharing mechanism,called BP2P-FL,is proposed using peer-to-peer federated learning with the privacy protection of data providers.In addition,by introducing the blockchain to the data sharing,every training process is recorded to ensure that data providers offer high-quality data.For further privacy protection,the differential privacy technology is used to disturb the global data sharing model.The experimental results show that BP2P-FL has high accuracy and feasibility in the data sharing of various IoT applications. | Qinyang Miao Hui Lin Jia Hu Xiaoding Wang | 2022 | Digital Communications and Networks2022，8，5： | 1 |
| 11 | Aggregation-induced emission luminogens reveal cell cycle-dependent telomerase activity in cancer cells显示文摘Telomerase acts as an important biomarker for tumor identification, and synthesizes telomeric repeats at the end of chromosome telomeres during the replicative phase of the cell cycle;thus, the expression level of telomerase changes as the cell cycle progresses. TERT m RNA expression and telomerase activity were significantly increased in over 80% of human cancers from tissue specimens. Although many efforts have been made in detecting the activity of TERT m RNA and active telomerase, the heterogeneous behavior of the cell cycle was overlooked, which might affect the accuracy of the detection results. Herein, the AIEgen-based biosensing systems of Py TPA-DNA and Silole-R were developed to detect the cellular level of TERT m RNA and telomerase in different cell cycles. As a result, the fluorescence signal of cancer cells gradually increased from G0/G1, G1/S to S phase. In contrast, both cancer cells arrested at G2/M phase and normal cells exhibited negligible fluorescence intensities. Compared to normal tissues, malignant tumor samples demonstrated a significant turn-on fluorescence signal. Furthermore, the transcriptomics profiling revealed that tumor biomarkers changed as the cell cycle progressed and biomarkers of CA9, TK1 and EGFR were more abundantly expressed at early S stage. In this vein, our study presented advanced biosensing tools for more accurate analysis of the cell-cycle-dependent activity of TERT m RNA and active telomerase in clinical tissue samples. | Xia Wu Jun Wu Jun Dai Biao Chen Zhe Chen Shixuan Wang Feng Wu Xiaoding Lou Fan Xia | 2021 | National Science Review2021，8，6： | 1 |
| 12 | Synthesis and properties of pH and temperature sensitive P (NIPAAm-co-DMAEMA) hydrogels显示文摘 | Wang Bo Xu Xiaoding Wang Zongchun | 2008 | Colloids and Surfaces B: Biointerfaces2008，64，： | 1 |
| 13 | Study on novel hydrogels based on thermosensifive PNIPAAm with PH sensitive PDMAEMA grafts显示文摘 | Wang Zongchun Xu Xiaoding Chert Changsheng | 2008 | Colloids and Surfaces B: Biointerfaces2008，67，： | 1 |
| 14 | Red AIE conjugated polyelectrolytes for long-term tracing and image-guided photodynamic therapy of tumors显示文摘Robust photosensitizers with strong red/NIR fluorescence, efficient reactive oxygen species(ROS) generation and high photostability are highly desired for photodynamic therapy(PDT). Herein, three novel red conjugated polyelectrolytes(CPEs) with tetraphenylethene and 2,1,3-benzothiadiazole on the main chains and triphenylphosphonium on the side chains are developed.These CPEs display apparent aggregation-induced emission feature and high fluorescence quantum yields in the aggregated state. They can target lysosome in He La cells for fluorescence bioimaging. By virtue of the good retention effect and high photostability, these CPEs show ultralong-term tracing performance of subcutaneous tumors, and the tumor site can still be visualized for 20 days after injection. Owing to their good biocompatibility and strong ROS generation ability, the image-guided PDT based on these CPEs can effectively inhibit the growth of subcutaneous tumor and significantly prolong the survival of tumor bearing mice. The H&E and IHC staining reveal that the PDT of these CPEs depress the proliferation of tumor cells, and promote apoptosis and necrosis process. These new CPEs may be employed both as fluorescent probes for in vitro and in vivo long-term tracing and as photosensitizers for image-guided PDT of tumors. | Hongming Yao Jun Dai Zeyan Zhuang Jinya Yao Zixuan Wu Shixuan Wang Fan Xia Jian Zhou Xiaoding Lou Zujin Zhao | 2020 | Science China Chemistry2020，63，12： | 1 |
| 15 | Nanoparticles(NPs)-mediated systemic mRNA delivery to reverse trastuzumab resistance for effective breast cancer therapy显示文摘Monoclonal antibody-based therapy has achieved great success and is now one of the most crucial therapeutic modalities for cancer therapy.The first monoclonal antibody authorized for treating human epidermal growth receptor 2(HER2)-positive breast cancer is trastuzumab.However,resistance to trastuzumab therapy is frequently encountered and thus significantly restricts the therapeutic outcomes.To address this issue,tumor microenvironment(TME)pH-responsive nanoparticles(NPs)were herein developed for systemic mRNA delivery to reverse the trastuzumab resistance of breast cancer(BCa).This nanoplatform is comprised of a methoxyl-poly(ethylene glycol)-b-poly(lactic-co-glycolic acid)copolymer with a TME pH-liable linker(Meo-PEG-Dlinkm-PLGA)and an amphiphilic cationic lipid that can complex PTEN mRNA via electrostatic interaction.When the long-circulating mRNA-loaded NPs build up in the tumor after being delivered intravenously,they could be efficiently internalized by tumor cells due to the TME pH-triggered PEG detachment from the NP surface.With the intracellular mRNA release to up-regulate PTEN expression,the constantly activated PI3K/Akt signaling pathway could be blocked in the trastuzumab-resistant BCa cells,thereby resulting in the reversal of trastuzumab resistance and effectively suppress the development of BCa. | Zhihui Dong Zhuoshan Huang Senlin Li Ying Wang Yandan Yao Xianzhu Yang Xiaoding Xu | 2023 | Acta Pharmaceutica Sinica B2023，13，3： | 1 |
| 16 | Ultrafast growth of wafer-scale fold-free bilayer graphene显示文摘Bilayer graphene provides a versatile platform for exploring a variety of intriguing phenomena and shows much promise for applications in electronics,optoelectronics,etc.Controlled growth of large-area bilayer graphene is therefore highly desired yet still suffers from a slow growth rate and poor layer uniformity.Meanwhile,graphene wrinkles,including folds and ripples,form during cooling due to the thermal contraction mismatch between graphene and the metal substrates,and have been far from suppressed or eliminated,especially in bilayer graphene,which would greatly degrade the extraordinary properties of graphene.Here we report the ultrafast growth of wafer-scale fold-free bilayer graphene by chemical vapor deposition.Through well-tuning the alloy thickness and strain regulation of the single-crystal CuNi(111)/sapphire,the full coverage of a 2-inch fold-free bilayer graphene wafer via mainly isothermal segregation has been achieved as fast as 30 s.The tensile-strained CuNi(111)film reduces the thermal contraction mismatch and suppresses the formation of graphene folds during cooling,which is directly observed through in situ optical microscopy.The ultraflat bilayer graphene exhibits wafer-scale uniformity in electrical performance and enhanced mechanical property comparable to the exfoliated ones.Our results offer a promising route for largescale production of bilayer graphene and enable its various applications. | Jilin Tang Yuechen Wang Yuwei Ma Xiaoyin Gao Xin Gao Ning Li Yani Wang Shishu Zhang Liming Zheng Bing Deng Rui Yan Yisen Cao Ronghua Zhang Lianming Tong Jin Zhang Peng Gao Zhongfan Liu Xiaoding Wei Hongtao Liu Hailin Peng | 2023 | Nano Research2023，16，7： | 0 |
| 17 | Genomic insights on the contribution of introgressions from Xian/Indica to the genetic improvement of Geng/Japonica rice cultivars显示文摘Hybridization between Xian/indica(XI)and Geng/japonica(GJ)rice combined with utilization of plant ideotypes has greatly contributed to yield improvements in modern GJ rice in China over the past 50 years.To explore the genomic basis of improved yield and disease resistance in GJ rice,we conducted a large-scale genomic landscape analysis of 816 elite GJ cultivars representing multiple eras of germplasm from China.We detected consistently increasing introgressions from three XI subpopulations into GJ cultivars since the 1980s and found that the XI genome introgressions significantly increased the grain number per panicle(GN)and decreased the panicle number per plant.This contributed to the improvement of plant type during modern breeding,changing multi-tiller plants tomoderate tiller plants with a large panicle size and increasing the blast resistance.Notably,we found that key gene haplotypes controlling plant architecture,yield components,and pest and disease resistance,including IPA1,SMG1,DEP3,Pib,Pi-d2,and Bph3,were introduced from XI rice by introgression.By GWAS analysis,we detected a GN-related gene Gnd5,which had been consistently introgressed from XI into GJ cultivars since the 1980s.Gnd5 is a GRAS transcription factor gene,and Gnd5 knockout mutants showed a significant reduction in GN.The estimated genetic effects of genes varied among different breeding locations,which explained the distinct introgression levels of XI gene haplotypes,including Gnd5,DEP3,etc.,to these GJ breeding pedigrees.These findings reveal the genomic contributions of introgressions from XI to the trait improvements of GJ rice cultivars and provide new insights for future rice genomic breeding. | Di Cui Han Zhou Xiaoding Ma Zechuan Lin Linhua Sun Bing Han Maomao Li Jianchang Sun Jin Liu Guixiu Jin Xianju Wang Guilan Cao Xing Wang Deng Hang He Longzhi Han | 2022 | Plant Communications2022，3，3： | 0 |
| 18 | One-dimensional and two-dimensional nanomaterials for the detection of multiple biomolecules显示文摘The complexity of biological samples determines that the detection of a single biomolecule is unable to satisfy actual needs. Moreover, the 'false positives' results caused by a single biomolecule detections easily leads to erroneous clinical diagnosis and treatment. Thus, it is important for the homogenous quantification of multiple biomolecules in not only basic research but also practical application. As a consequent, a large number of literatures have been exploited to monitor multiple biomolecules in homogenous solution, enabling facilitating the development of the disease diagnosis, treatment as well as drug discovery. One-dimensional nanomaterials and two-dimensional nanomaterials have special physical and chemical properties, such as good electrochemical properties, stable structure, large specific surface area, and biocompatibility, which are widely used in electrochemical and fluorescent detection of biomolecules. This tutorial review highlights the recent development for the detection of multiple biomolecules by using nanomaterials including one-dimensional materials(1DMs) as well as twodimensional materials(2DMs). | Quan Wang Xudong Wang Min Xu Xiaoding Lou Fan Xia | 2019 | Chinese Chemical Letters2019，30，9： | 0 |
| 19 | The Degradation of the Public Virtue and the American Transformation to a Modern Commercial and Industrial Society显示文摘Many of the founding fathers were greatly influenced by the classical republicanism. They adhered to the ethical norms based on the public virtues advocated in the Renaissance Era, constantly watched out the corruption from power and strove to avoid excessive expansion of wealth which might bring about the disintegration of social cohesion. Therefore, they laid emphasis on the importance of the public virtues in the development of this newly independent country. However, their classical republic ideas were obviously incompatible with the trend of American development after independence. Thus many of them experienced a course of gradual identification with modern industrial civilization. As a result, this identification promoted the United States to march toward a society of commerce and industry in great stride. | Wang Xiaode | 2015 | World History Studies2015，2，2： | 0 |
| 20 | Study on a Fuzzy Reasoning Algorithm显示文摘This paper presents a kind of inexact inference methodology, and gives a calculationalgorithm. The algorithm can be distinguished not only between exact and inexact inference, butalso in conditions of inference between many and few inferences. | Liang, Yun Zuo, Xiaode Sun, Xianjin Wang, Huifen Hu, Dongpo | 1999 | Journal of Systems Engineering and Electronics1999，10，2： | 0 |
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