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| 1 | Screening Helicobacter pylori genes induced during infection of mouse stomachs显示文摘AIM:To investigate the effect of in vivo environment on gene expression in Helicobacter pylori(H.pylori) as it relates to its survival in the host.METHODS:In vivo expression technology(IVET) systems are used to identify microbial virulence genes.We modified the IVET-transcriptional fusion vector,pIVET8,which uses antibiotic resistance as the basis for selection of candidate genes in host tissues to develop two unique IVET-promoter-screening vectors,pIVET11 and pIVET12.Our novel IVET systems were developed by the fusion of random Sau3A DNA fragments of H.pylori and a tandem-reporter system of chloramphenicol acetyltransferase and beta-galactosidase.Additionally,each vector contains a kanamycin resistance gene.We used a mouse macrophage cell line,RAW 264.7 and mice,as selective media to identify specific genes that H.pylori expresses in vivo.Gene expression studies were conducted by infecting RAW 264.7 cells with H.pylori.This was followed by real time polymerase chain reaction(PCR) analysis to determine the relative expression levels of in vivo induced genes.RESULTS:In this study,we have identified 31 in vivo induced(ivi) genes in the initial screens.These 31 genes belong to several functional gene families,including several well-known virulence factors that are expressed by the bacterium in infected mouse stomachs.Virulence factors,vacA and cagA,were found in this screen and are known to play important roles in H.pylori infection,colonization and pathogenesis.Their detection validates the efficacy of these screening systems.Some of the identified ivi genes have already been implicated to play an important role in the pathogenesis of H.pylori and other bacterial pathogens such as Escherichia coli and Vibrio cholerae.Transcription profiles of allivi genes were confirmed by real time PCR analysis of H.pylori RNA isolated from H.pylori infected RAW 264.7 macrophages.We compared the expression profile of H.pylori and RAW 264.7 coculture with that of H.pylori only.Some genes such as cag A,vac A,lpx C,mur I,tlp C,trx B,sod B,tnp B,pgi,rbf A and inf B showed a 2-20 fold upregulation.Statistically significant upregulation was obtained for all the above mentioned genes(P < 0.05).tlp C,cag A,vac A,sod B,rbf A,inf B,tnp B,lpx C and mur I were also significantly upregulated(P < 0.01).These data suggest a strong correlation between results obtained in vitro in the macrophage cell line and in the intact animal.CONCLUSION:The positive identification of these genes demonstrates that our IVET systems are powerful tools for studying H.pylori gene expression in the host environment. | Aparna Singh Nathaniel Hodgson Ming Yan Jungsoo Joo Lei Gu Hong Sang Emmalena Gregory-Bryson William G Wood Yisheng Ni Kimberly Smith Sharon H Jackson William G Coleman | 2012 | World Journal of Gastroenterology2012，18，32： | 5 |
| 2 | Fabrication of SAW Sensor for Detecting Chemical Agent显示文摘SAW sensors using five different types of polymer to detect of chemical agents(DMMP,CH_3CN,CH_2Cl_2,DCP) have been fabricated and its gas response characteristics were extensively investigated.The polymers used as the sensing material are polyisobutylene(PIB),polyepichlorohydrin(PECH),polydimethylsiloxane(PDMS),polyisoprene(PIP)and polybutadiene(PBD).Their thin films were coated on quartz substrate by spin coating technique.Three types of simulants gases,dimethylmethylphosphonate(DMMP),acetonitrile(CH_3CN)and dichloromethane(CH_2Cl_2),dichloropentane(DCP)were used as target gases,instead of the real nerve,blood,choking and vesicant agents.After spin coating of PIB and PECH,the substrates were heated to 65℃with N_2 flow for 1 h to remove the cyclohexane and ethylacetate which was used as solvent. PDMS was heated to 75℃with N_2 flow for 2 h to remove the ethylacetate which was used as solvent.PBD and PIP on the substrate were heated to 60℃with N_2 flow for 1 h to remove the benzen which was used as solvent.The sensing characteristics of the SAW sensors were measured by using E-5061A network analyzer. | J H Lee B S Joo J S Huh D D Lee | 2006 | 稀有金属材料与工程2006，35，A03： | 3 |
| 3 | Porcine parvovirus DNA:charaeterization of the genomie and replieation form DNA of two viruses isolates显示文摘 | Molitor T W Joo H S Collett M S | 1984 | Virology1984，137，2： | 1 |
| 4 | Robust Control for Uncertain Takagi-Sugeno Fuzzy Systems with Time-varying Input Delay显示文摘 | Lee H J Park J B Joo Y H | 2005 | Journal of Dynamic Systems Measurement and Control2005，127，2： | 1 |
| 5 | Synthesis of highly ordered carbon molecular sieves via template-mediated structural transformation 显示文摘 | Ryoo R Joo S H Jun S | 1999 | J Phys Chem B1999，103，37： | 1 |
| 6 | Optimization of supereritieal water oxidation (scwo) process for decomposing nitromethane 显示文摘 | JOO H CHANG M SEUNG H | 2006 | Industrial & Engineering Chemistry Research2006，44，6： | 1 |
| 7 | Expression of vascular endothelial growth factor and inducible nitric oxide synthase in pterygia显示文摘 | Lee DH Cho H J Kim JT Choi JS Joo CK | 2001 | Cornea2001，20，7： | 1 |
| 8 | Energetically favored formation of MCM-48 from cationic-neutral surfactant mixtures 显示文摘 | RYOO R JOO S H KIM J M | 1999 | J Phys Chem B1999，103，35： | 1 |
| 9 | Decay of radical initiators in emulsion copolymerization of unsaturated polyesters and styrene显示文摘 | Bauer H Ortelt M Joos B | 1988 | Die Makromolekulare Chemie1988，189，2： | 1 |
| 10 | Auxin-induced reactive oxygen species production requires the activation of phosphatidylinositol 3 - kinase 显示文摘 | Joo J H Yoo H J Hwang I | 2005 | FEBS Letters2005，579，： | 1 |
| 11 | Prognostic significance of p53 and p21 expression in cancer显示文摘 | Seo Y H Joo Y E Choi S K | 2003 | Korean J Intern Med2003，18，2： | 1 |
| 12 | Lin28 mediates the ter-minal uridylation of let-7 precursor MicroRNA 显示文摘 | Heo h Joo C Cho J | 2008 | Mol Cell2008，32，2： | 1 |
| 13 | Sevoflurance versus propofol for anes- thesia induction : a meta-analysis 显示文摘 | Joo H S Perks W J | 2000 | Anesth Analg2000，91，1： | 1 |
| 14 | Digitalizing a fuzzy obseverbased out put-feedback control:intelligent digital redesign approach显示文摘 | Lee H J Park J B Joo Y H | | 0，，10： | 1 |
| 15 | 查看详情显示文摘 | Jung W Uk Joo H | | 0，，： | 1 |
| 16 | Thermomechanical properties of brazed wire-woven bulk Kagome cellular metals for multifunctional applications显示文摘 | FENG S S LI M Z JOO J H | 2012 | Journal of Thermophysics and Heat Transfer2012，26，1： | 1 |
| 17 | Design and analysis of the nonlinear feedback linearizing control for an electromagnetic suspension system显示文摘 | Joo S J Seo J H | 1997 | IEEE Transactions on Control Systems Technology1997，5，1： | 1 |
| 18 | Role of auxin-induced reactive oxygen species in root gravitropism显示文摘 | Joo J H Bae Y S Lee J S | 2001 | Plant Physiology2001，126，3： | 1 |
| 19 | Development of high-driving potential and high-efficiency Mg-based sacrificial anodes for cathodic protection显示文摘 | KIM J G JOO J H KOO S J | 2000 | Journal of Materials Science Letters2000，19，6： | 1 |
| 20 | Synthesis of new, nanoporous carbon with hexagonally ordered mesostructure显示文摘 | Jun S Joo S H Ryoo R | 2000 | J Am Chem Soc2000，122，10： | 1 |
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