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| 1 | 用RFLP方法研究陕西省主要小麦品种遗传多样性及其演变显示文摘研究用RFLP方法,对我国主要小麦产区,陕西省40年代以来广为种植的小麦品种的遗传多样性进行了研究,结果表明,占总带数26.2%的HINDII酶的RFLP带具有遗传多态性。24个小麦品种平均遗传相似系数为0.948。53.8%的探针显示了多态性。平均多态性信息含量指数为0.252。陕西省小麦品种遗传多样性以70年代为最高,之后开始降低。 | 朱列层 唐国顺 HAZEN S P KIM H S WARD R W | 1999 | 西北植物学报1999，19，2： | 11 |
| 2 | Sodium glucose co-transporter 2 inhibition reduces succinate levels in diabetic mice显示文摘BACKGROUND Type 1 diabetes(T1D) is associated with major chronic microvascular complications which contribute significantly to diabetes associated morbidity.The protein primarily responsible for glucose reabsorption in the kidney is sodium glucose co-transporter 2(SGLT2). Presently, SGLT2 inhibitors are widely used in diabetic patients to improve blood glucose levels and prevent cardiovascular and renal complications. Given the broad therapeutic application of SGLT2 inhibitors, we hypothesised that SGLT2 inhibition may exert its protective effects via alterations of the gut microbiome and tested this in a type 1 diabetic mouse model of diabetic retinopathy.AIM To determine whether the treatment with two independent SGLT2 inhibitors affects gut health in a type 1 diabetic mouse model.METHODS The SGLT2 inhibitors empagliflozin or dapagliflozin(25 mg/kg/d) or vehicle dimethylsulfoxide(DMSO) were administered to C57 BL/6 J, Akita, Kimba and Akimba mice at 10 wk of age for 8 wk via their drinking water. Serum samples were collected and the concentration of succinate and the short chain fatty acid(SCFA) butyric acid was measured using gas chromatography-mass spectrometry. Enzyme-linked immunosorbent assay(ELISA) was performed to determine the concentration of insulin and leptin. Furthermore, the norepinephrine content in kidney tissue was determined using ELISA. Pancreatic tissue was collected and stained with haematoxylin and eosin and analysed using brightfield microscopy.RESULTS Due to the presence of the Akita allele, both Akita and Akimba mice showed a reduction in insulin production compared to C57 BL/6 J and Kimba mice.Furthermore, Akita mice also showed the presence of apoptotic bodies within the pancreatic islets. The acinar cells of Akita and Akimba mice showed swelling which is indicative of acute injury or pancreatitis. After 8 wk of SGLT2 inhibition with dapagliflozin, the intermediate metabolite of gut metabolism known as succinate was significantly reduced in Akimba mice when compared to DMSO treated mice. In addition, empagliflozin resulted in suppression of succinate levels in Akimba mice. The beneficial SCFA known as butyric acid was significantly increased in Akita mice after treatment with dapagliflozin when compared to vehicle treated mice. The norepinephrine content in the kidney was significantly reduced with both dapagliflozin and empagliflozin therapy in Akita mice and was significantly reduced in Akimba mice treated with empagliflozin.In non-diabetic C57 BL/6 J and Kimba mice, serum leptin levels were significantly reduced after dapagliflozin therapy.CONCLUSION The inhibition of SGLT2 reduces the intermediate metabolite succinate, increases SCFA butyric acid levels and reduces norepinephrine content in mouse models of T1 D. Collectively, these improvements may represent an important mechanism underlying the potential benefits of SGLT2 inhibition in T1 D and its complications. | Lakshini Y Herat Natalie C Ward Aaron L Magno Elizabeth P Rakoczy Marcio G Kiuchi Markus P Schlaich Vance B Matthews | 2020 | World Journal of Gastroenterology2020，26，23： | 3 |
| 3 | Laryngeal and oropharyngeal cancer, and alcohol dehydrogenase 3 and glutathione S-transferase M1 polymorphisms显示文摘 | Coutelle C Ward PJ Fleury B Quattrocchi P Chambrin H Iron A | 1997 | Hum Genet1997，99，3： | 3 |
| 4 | 超顺磁性氧化铁(SPIO)对比剂肝脾MR成像的比较研究显示文摘目的 比较两种超顺磁性氧化铁 (superparamagneticironoxide ,SPIO)对比剂 ,Ferumoxides及SHU 5 5 5A在肝脾MR成像中的效应。材料与方法 3 6例已知为肝转移癌患者于SPIO造影前后进行T2 WI快速自旋回波成像 (T2 WITSE)及T1WI梯度回波快速去相位成像 (T1WIFLASH)。扫描仪为 1.0TMR机。 18例患者行Ferumoxides增强后 90分钟进行MR成像 ;另 18例行SHU 5 5 5A快速团注增强 ,注药后即刻、3 0秒及 480秒行T1WIFLASH成像 ,10分钟行T2 WITSE成像。测量肝脾、肝转移癌SPIO增强前后的信号强度(signalintensity ,SI) ,计算两种SPIO对比剂在肝脾、肝转移癌增强前后SI变化的百分比 (percentagesignalintensitychange ,PSIC)及病灶 肝脏对比噪声比 (lesion to livercontrast to noiseratio ,CNR)及其变化 (ΔCNR)。结果 在T2 WITSE图像上 ,两种SPIO对比剂造成的肝实质SI下降无显著性差异 (P >0 .0 5 )。Ferumoxides引起的脾信号下降显著大于SHU 5 5 5A(P <0 .0 5 )。两种SPIO对比剂均导致肝转移癌CNR显著增高。T1WIFLASH图像上 ,两种对比剂均可导致延迟像上肝脏SI的轻度下降及肝转移癌CNR下降 ,两者肝脏PSIC之间无显著性差异。T1WI上两种对比剂均可导致脾脏SI显著升高 ,两者脾脏PSIC之间无显著性差异 (P >0 .0 5 ) | 陈峰 Ward J Robinson P J | 2001 | 临床放射学杂志2001，20，3： | 3 |
| 5 | Factors affecting recovery and quality of oocytes for bovine embryo production in vitro using ovum pick-up technology显示文摘 | Ward F A Lonergan P Enright B P | 2000 | Theriogenology2000，54，： | 2 |
| 6 | Causes of encephalitis and differences in their clinical presentations in England: a multicentre, population-based prospective study显示文摘 | Julia Granerod Helen E Ambrose Nicholas WS Davies Jonathan P Clewley Amanda L Walsh Dilys Morgan Richard Cunningham Mark Zuckerman Ken J Mutton Tom Solomon Katherine N Ward Michael PT Lunn Sarosh R Irani Angela Vincent David WG Brown Natasha S Crowcroft | 2010 | The Lancet Infectious Diseases2010，，12： | 2 |
| 7 | The Establishment of ultraviolet spectral emission scales using synchrotron radiation显示文摘 | KEY P J WARD T H | 1978 | Metrologia1978，14，1： | 2 |
| 8 | UK and Ireland TVT trial group:tension- free vaginal tape versus colposuspension for primary urodynamic stress incontinence : 5-year follow up 显示文摘 | Ward KL Hilton P | 2008 | BJOG2008，115，2： | 1 |
| 9 | Review multiple sequence alignment in phylogenetic analysis显示文摘 | Aloysius P Daniel J Ward W | 2000 | Molecular Phylogenetics and Evolution2000，16，3： | 1 |
| 10 | Extent of pulmonary tuberculosis in patients diagnosed by active compared to passive case finding显示文摘 | Ward HA Marciniuk DD Pahwa P | 2004 | Int Tuberc Lung Dis2004，8，5： | 1 |
| 11 | Production of high yield of arachidonic acid in a fed batch system by Mortierella alpina ATCC 32222显示文摘 | WARD O P | 1997 | Appl Microbiol Biotechnol1997，48，7： | 1 |
| 12 | Negative regulation of macrophage activation in response to IFN-γ and lipopolysaccharide by the STK/RON receptor tyrosine kinase显示文摘 | Ward J Correll P H | 1999 | J Immunol1999，163，： | 1 |
| 13 | Optical harmonics and nonlinear phenomena显示文摘 | Ward J F | 1963 | Rev Mod Phys1963，35，： | 1 |
| 14 | improved survival in patients with locally advanced prostate cancer treated with radiotherapy and goserelin 显示文摘 | Bolla M Gonzalez D Warde P | 1997 | N EngtJ Med1997，337，5： | 1 |
| 15 | Effect of culture conditions on growth and sporulation of Bacillus thuringiensis subsp israelensis and development of media for production of the protein Crystal endotoxin显示文摘 | PEARSON D WARD O P | 1988 | Biotechnology Letters1988，10，7： | 1 |
| 16 | A novel plasminogen activator from Streptococcus uberis显示文摘 | Rosey E L Lincoln R A Ward P N | 1999 | FEMS Microbiol Lett1999，178，： | 1 |
| 17 | Prospects for expanded utilization of biogas in Germany显示文摘 | POESCHL M WARD S OWENDE P | 2010 | Renewable and Sustainable Energy Reviews2010，14，7： | 1 |
| 18 | Mammary gland mophogenesis is enhanced by exposure to flaxseed or its major lignan during suckling in rats显示文摘 | TAN K P CHEN J WARD W E | 2004 | Exp Biol Med2004，229，： | 1 |
| 19 | Arrhythmogenic fight ventrieular dysplasia/cardiomyopathy associated with mutations in the desmosomal gene desmocollin-2显示文摘 | Syrris P Ward D Evans A | 2006 | Am J Hum Genet2006，79，5： | 1 |
| 20 | Patient Participation in Decision Making 显示文摘 | Guadagnoli E Ward P | 1998 | SocSciMed1998，47，3： | 1 |
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