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| 1 | Apoptosis and its relationship with cell proliferation,p53,Waf1p21,bcl-2 and c-mycin esophageal carcinogenesis studied with a high-risk population in northern China显示文摘Apoptosisanditsrelationshipwithcelproliferation,p53,Waf1p21,bcl2andcmycinesophagealcarcinogenesisstudiedwithahighriskpopul... | WANG LiDong1, ZHOU Qi1, WEI JunPing1, YANG WanCai1, ZHAO Xin1, WANG LiXia1, ZOU Jian Xiang1, GAO ShanShan1, LI YongXin1 and YANG C.S.2Keywords esophageal neoplasms apoptosis precancerous lesions Waf1 p21 gene p53 | 1998 | World Journal of Gastroenterology1998，4，4： | 295 |
| 2 | Mammalian WTAP is a regulatory subunit of the RNA N6-methyladenosine methyltransferase显示文摘包含 methyltransferase 建筑群的象 3 一样的 methyltransferase (METTL3 ) 催化 N6-methyladenosine (m6A ) 形成,一个新奇 epitranscriptomic 标记;然而,这建筑群的性质仍然保持大部分未知。这里,我们报导人的 m6A methyltransferase 建筑群, Wilm 的肿瘤 1 伙伴蛋白质(WTAP ) 和象 14 一样的 methyltransferase (METTL14 ) 的二个新部件。WTAP 与 METTL3 和 METTL14 交往,并且为他们的本地化被要求进在 vivo 与处理因素的 pre-mRNA 并且为 m6A methyltransferase 的催化活动充实的原子点缀。RNA 的多数在 vivo 由 WTAP 和 METTL3 跳了代表包含一致 m6A 主题的 mRNAs。当 WTAP 不在时, METTL3 的 RNA 有约束力的能力强烈被减少,建议 WTAP 可以工作调整到 mRNA 目标的 m6A methyltransferase 建筑群的招募。而且,在有 photoactivatable-ribonucleoside-enhanced crosslinking 和 immunoprecipitation (同等片断) 的联合的 transcriptomic 分析说明那 WTAP 和 METTL3 调整涉及抄写并且 RNA 处理的基因拼接的表示和选择。在 zebrafish 胚胎的调停 Morpholino 的击倒的指向 WTAP 或 METTL3 引起了织物区别缺点并且增加了 apoptosis。这些调查结果提供 WTAP 可以在 m6A methyltransferase 建筑群作为一个规章的子单元工作并且在 RNA 的 epitranscriptomic 规定起一个关键作用的充分证据新陈代谢。 | Xiao-Li Ping Bao-Fa Sun Lu Wang Wen Xiao Xin Yang Wen-Jia Wang Samir Adhikari Yue Shi Ying Lv Yu-Sheng Chen Xu Zhao Ang Li Ying Yang Ujwal Dahal Xiao-Min Lou Xi Liu Jun Huang Wei-Ping Yuan Xiao-Fan Zhu Tao Cheng Yong-Liang Zhao Xinquan Wang Jannie M Rendtlew Danielsen Feng Liu Yun-Gui Yang | 2014 | Cell Research2014，24，2： | 244 |
| 3 | FTO-dependent demethylation of N6-methyladenosine regulates mRNA splicing and is required for adipogenesis显示文摘 | Xu Zhao Ying Yang Bao-Fa Sun Yue Shi Xin Yang Wen Xiao Ya-Juan Hao Xiao-Li Ping Yu-Sheng Chen Wen-Jia Wang Kang-Xuan Jin Xing Wang Chun-Min Huang Yu Fu Xiao-Meng Ge Shu-Hui Song Hyun Seok Jeong Hiroyuki Yanagisawa Yamei Niu Gui-Fang Jia Wei Wu Wei-Min Tong Akimitsu Okamoto Chuan He Jannie M Rendtlew Danielsen Xiu-Jie Wang Yun-Gui Yang | 2014 | Cell Research2014，24，12： | 109 |
| 4 | Cytoplasmic m6A reader YTHDF3 promotes mRNA translation显示文摘 | Ang Li Yu-Sheng Chen Xiao-Li Ping Xin Yang Wen Xiao Ying Yang Hui-Ying Sun Qin Zhu Poonam Baidya Xing Wang Devi Prasad Bhattarai Yong-Liang Zhao Bao-Fa Sun Yun-Gui Yang | 2017 | Cell Research2017，27，3： | 82 |
| 5 | Extensive translation of circular RNAs driven by N6-methyladenosine显示文摘广泛的 pre-mRNA 拼接背在人的 transcriptome 产生众多的圆形的 RNA (circRNAs ) 。然而,这些 circRNAs 的生物功能仍然保持大部分不清楚。这里,我们报导那 N 6-methyladenosine (m 6 一) , RNA 的最丰富的基础修正,在人的房间从 circRNAs 支持蛋白质翻译的有效开始。我们发现那一致 m 6 A 主题在 circRNAs 和单个 m 6 一个地点是足够的驾驶翻译开始。这 m 6A 驱动的翻译要求开始因素 eIF4G2 和 m 6 读者 YTHDF3,并且被 methyltransferase METTL3/14 提高,在热吃惊之上由 demethylase FTO,和 upregulated 禁止了。通过介绍的 polysome 的进一步的分析,计算预言和集体 spectrometry 揭示那 m 6 circRNAs 的 A 驱动的翻译是普遍的,与有翻译潜力的几百内长的 circRNAs。我们的学习扩展人的 transcriptome 的编码风景,并且在对环境应力的细胞的回答建议导出 circRNA 的蛋白质的一个角色。 | Yun Yang Xiaojuan Fan Miaowei Mao Xiaowei Song Ping Wu6,7 Yang Zhang Yongfeng Jin Yi Yang Ling-Ling Chen Yang Wang Catherine CL Wong Xinshu Xiao Zefeng Wang | 2017 | Cell Research2017，27，5： | 246 |
| 6 | Stable classi?cation with limited sample: transferring a 30-m resolution sample set collected in 2015 to mapping 10-m resolution global land cover in 2017显示文摘As the world strives to reduce the impact of population growth, urbanization, agricultural expansion, and climate change on food security, energy and water shortage, resource over-exploration, biodiversity loss, environmental pollution, and ultimately human health, timely and higher resolution land cover information is urgently needed to achieve the sustainable development goals of the United Nations. | Peng Gong Han Liu Meinan Zhang Congcong Li Jie Wang Huabing Huang Nicholas Clinton Luyan Ji Wenyu Li Yuqi Bai Bin Chen Bing Xu Zhiliang Zhu Cui Yuan Hoi Ping Suen Jing Guo Nan Xu Weijia Li Yuanyuan Zhao Jun Yang Chaoqing Yu Xi Wang Haohuan Fu Le Yu Iryna Dronova Fengming Hui Xiao Cheng Xueli Shi Fengjin Xiao Qiufeng Liu Lianchun Song | 2019 | Science Bulletin2019，64，6： | 166 |
| 7 | Evolution of the novel coronavirus from the ongoing Wuhan outbreak and modeling of its spike protein for risk of human transmission显示文摘Dear Editor,The occurrence of concentrated pneumonia cases in Wuhan city,Hubei province of China was first reported on December 30,2019 by the Wuhan Municipal Health Commission(WHO,2020).The pneumonia cases were found to be linked to a large seafood and animal market in Wuhan,and measures for sanitation and disinfection were taken swiftly by the local government agency.The Centers for Disease Control and Prevention(CDC)and Chinese health authorities later determined and announced that a novel coronavirus(CoV),denoted as 2019-nCoV,had caused the pneumonia outbreak in Wuhan city(CDC,2020).Scientists from multiple groups had obtained the virus samples from hospitalized patients(Normile,2020).The isolated viruses were morphologically identical when observed under electron microscopy. | Xintian Xu Ping Chen Jingfang Wang Jiannan Feng Hui Zhou Xuan Li Wu Zhong Pei Hao | 2020 | Science China(Life Sciences)2020，63，3： | 737 |
| 8 | Towards 6G wireless communication networks:vision,enabling technologies,and new paradigm shifts显示文摘The fifth generation(5G)wireless communication networks are being deployed worldwide from 2020 and more capabilities are in the process of being standardized,such as mass connectivity,ultra-reliability,and guaranteed low latency.However,5G will not meet all requirements of the future in 2030 and beyond,and sixth generation(6G)wireless communication networks are expected to provide global coverage,enhanced spectral/energy/cost efficiency,better intelligence level and security,etc.To meet these requirements,6G networks will rely on new enabling technologies,i.e.,air interface and transmission technologies and novel network architecture,such as waveform design,multiple access,channel coding schemes,multi-antenna technologies,network slicing,cell-free architecture,and cloud/fog/edge computing.Our vision on 6G is that it will have four new paradigm shifts.First,to satisfy the requirement of global coverage,6G will not be limited to terrestrial communication networks,which will need to be complemented with non-terrestrial networks such as satellite and unmanned aerial vehicle(UAV)communication networks,thus achieving a space-airground-sea integrated communication network.Second,all spectra will be fully explored to further increase data rates and connection density,including the sub-6GHz,millimeter wave(mmWave),terahertz(THz),and optical frequency bands.Third,facing the big datasets generated by the use of extremely heterogeneous networks,diverse communication scenarios,large numbers of antennas,wide bandwidths,and new service requirements,6G networks will enable a new range of smart applications with the aid of artificial intelligence(AI)and big data technologies.Fourth,network security will have to be strengthened when developing 6G networks.This article provides a comprehensive survey of recent advances and future trends in these four aspects.Clearly,6G with additional technical requirements beyond those of 5G will enable faster and further communications to the extent that the boundary between physical and cyber worlds disappears. | Xiaohu YOU Cheng-Xiang WANG Jie HUANG Xiqi GAO Zaichen ZHANG Mao WANG Yongming HUANG Chuan ZHANG Yanxiang JIANG Jiaheng WANG Min ZHU Bin SHENG Dongming WANG Zhiwen PAN Pengcheng ZHU Yang YANG Zening LIU Ping ZHANG Xiaofeng TAO Shaoqian LI Zhi CHEN Xinying MA Chih-Lin I Shuangfeng HAN Ke LI Chengkang PAN Zhimin ZHENG Lajos HANZO Xuemin(Sherman)SHEN Yingjie Jay GUO Zhiguo DING Harald HAAS Wen TONG Peiying ZHU Ganghua YANG Jun WANG Erik GLARSSON Hien Quoc NGO Wei HONG Haiming WANG Debin HOU Jixin CHEN Zhe CHEN Zhangcheng HAO Geoffrey Ye LI Rahim TAFAZOLLI Yue GAO HVincent POOR Gerhard P.FETTWEIS Ying-Chang LIANG | 2021 | Science China(Information Sciences)2021，64，1： | 122 |
| 9 | The Global Boundary Stratotype Section and Point (GSSP) for the base of the Hirnantian Stage (the uppermost of the Ordovician System)显示文摘 | Xu Chen Jiayu Rong Junxuan Fan Renbin Zhan Charles E. Mitchell David A. T. Harper Michael J. Melchin Ping'an Peng Stan C. Finney Xiaofeng Wang | 2006 | Episodes2006，29，3： | 61 |
| 10 | Effects of several amendments on rice growth and uptake of copper and cadmium from a contaminated soil显示文摘在可变费用土壤的重金属是高度对进植物的转移可得到、容易的简历。因为完全消除在污染土壤上种的大米是不可能的,一些补习和缓解技术是必要的由大米减少金属简历可获得性和举起。这盆栽试验从被铜和镉污染的水稻土在米饭的生长和重金属的举起上调查了七个修正案的效果。结果从增加了谷物的石灰石的申请的最好在谷物由 12.5 16.5 褶层,和减少的 Cu 和 Cd 集中让步由 23.0%50.4% 。钙镁磷酸盐,钙硅酸盐,猪粪肥,和泥炭的申请也由 0.3 15.3 褶层增加了谷物收益,并且有效地减少了在谷物的 Cu 和 Cd 集中。在谷物的 Cd 集中稍微在紫云英和锌硫酸盐的处理被减少。在谷物和稻草的 Cu 和 Cd 的集中在土壤依赖于可得到的 Cu 和 Cd,并且玷污可得到的 Cu 和 Cd 被土壤 pH 显著地影响。 | LI Ping WANG Xingxiang ZHANG Taolin ZHOU Dongmei HE Yuanqiu | 2008 | Journal of Environmental Sciences2008，20，4： | 72 |
| 11 | Analysis of in vivo patterns of caspase 3 gene expression in primary hepatocellular carcinoma and its relationship to p21^(WAF1) expression and hepatic apoptosis显示文摘AIM To detect the expression of caspase 3gene in primary human hepatocellular carcinoma(HCC)and investigate its relationship to p21WAF1gene expression and HCC apoptosis.METHODS In situ hybridization was employedto determine caspase 3 and p21WAF1expression inHCC.In situ end-labeling was used to detecthepatocytic apoptosis in HCC.RESULTS Twenty-one of 39(53.8%)cases ofHCC were found to express caspase 3transcripts,while 45.2% of HCC failed toexpress caspase 3.Non-cancerous adjacent livertissues showed more positive caspase 3(87.5%,7/8)as compared with HCC(P<0.05).The expression of caspase 3 is correlated withHCC differentiation,72.2%(13/18)ofmoderately to highly differentiated HCC showedcaspase 3 transcripts positive,while only 38.1%of poorly differentiated HCC harbored caspase 3transcripts(P<0.05).No relationship wasfound between caspase 3 expression and tumorsize or grade or metastasis,although 52.5%(5/8)of HCC with metastasis were caspase 3positive and a little higher than that with nometastasis(51.6%,P>0.05).Expression of caspase 3 alone did not affect the apoptosisindex(AI)of HCC.The AI was 7.12%o in caspase3-positive tumors(n=21),while in caspase 3-negative cases(n=18)6.59%0(P>0.05).Expression of caspase 3 clearly segregated withp21WAF1positive tumors as compared withp21WAF1-negative cases(16 of 23,69.6% versus5 of 16,31.3%)with statistical significance(P=0.017).In the cases with positive caspase 3and negative p21WAF1,the Al was found slightlyhigher,but with no statistical significance,thanthat with expression of p21WAF1and caspase 3(7.21‰ vs 6.98‰,P>0.05).CONCLUSION Loss of caspase 3 expressionmay contribute to HCC carcinogenesis,althoughthe expression of caspase 3 does not correlatewell with cell apoptosis in HCC.p21WAF1may bemerely one of the inhibitors which can reducecaspase 3 mediated cell apoptosis in HCCs. | Bao Hua Sun Jun Zhang Bao JǜWang Xi Ping Zhao You Kun Wang Zhi Qun Yu Dong Liang Yang Lian Jie Hao Department of Clinical Immunology,Tongji Hospital,Tongji Medical University,Wuhan 430030,Hubei Province,China | 2000 | World Journal of Gastroenterology2000，6，3： | 65 |
| 12 | Expression of nuclear factor-KB in hepatocellular carcinoma and its relation with the X protein of hepatitis B virus显示文摘AIM In this study we investigated therelationship of the X protein of HBV and nuclearfactor-KB (NF-κB) and the expression of NF-KB inhuman hepatocellular carcinoma tissues.METHODS Immunohistochemistry SP methodwas used to detect the expression of NF-κB and the X protein of HBV in human hepatocellularcarcinoma tissues of 52 cases. Gene transfectionmediated by lipofectamine was used to transfectthe eukaryotic expression vector pCDNA3. 1-HBXof HBV x gene into human hepatocellularcarcinoma cell line HCC-9204 and NF-κB wasdetected.RESULTS NF-κB was widely expressed inhuman hepatocellular carcinoma tissues in atotal of 52 cases and its expression was relatedto the X protein of HBV. NF-KB was localizedboth in the cytoplasm and . The nuclei ofhepatocellular carcinoma cells in 11 cases whichwere positive for the X protein of HBV while in 41cases negative for the X protein of HBV, NF-Kbwas only localized in the cytoplasm ofhepatocellular carcinoma cells but translocatedto the nuclei of hepatocellular carcinoma cellsafter the eukaryotic expression vectorpCDNA3.1-HBX was transfected into HCC-9204cells.CONCLUSION This study strongly suggeststhat the nuclear factor NF-KB is widely expressedin hepatocellular carcinoma tissues in differentstyles according to the expression of the Xprotein of HBV. NF-κB is abnormally activated inhepatocellular carcinoma, which is probablyrelated to the X protein of HBV. The X protein ofHBV can activate NF-κB to translocate into nucleiof hepatocellular carcinoma cells. | Shuang Ping Guo~1 Wen Liang Wang~1 Yu Qiang Zhai~2 Yi Ling Zhao~1 ~1Department of Pathology,Xijing Hospital of the Fourth Military Medical University,Xi’an,China ~2Department of Urology,the Central Hospital of Xi’an,China | 2001 | World Journal of Gastroenterology2001，7，3： | 55 |
| 13 | Treatment of gastric varices by endoscopic sclerotherapy using butyl cyanoacrylate:10 years' experience of 635 cases显示文摘背景胃的静脉曲张( GV )为有有丁基 cyanoacrylate ( BC )的门 hypertension.Endoscopic 注射的病人是威胁生活的,为 GV 的治疗的支柱,被报导了为为它在病人的幸存上的 GV 和影响的涂去的功效仍然需要的 varices.but 放血的 hemostasis 有效我们总结了的 clarification.Here 我们 10 years'practice 到的经验从 Januar 为 GV patients.Methods 用 BC | CHENG Liu-fang WANG Zhi-qiang LI Chang-zheng CAI Feng-chun HUANG Qi-yang LINGHU En-qiang LI Wen CHAI Guo-jun SUN Guo-hui MAO Yong-ping WANG Yan-mei LI Jing GAO Ping FAN Tie-yan | 2007 | Chinese Medical Journal2007，，23： | 51 |
| 14 | Relationships among hope, coping style and social support for breast cancer patients显示文摘背景乳癌是在女人的最普通的癌症之一,并且它的发生似乎每年逐渐地增加了。在乳癌的治疗期间,病人们承受心理病态,并且希望是在维持心理健康的一个重要因素。在这研究,因此,我们在化疗期间与乳癌在中国女人调查了希望的水平并且在希望之中证实了关系,处理风格,并且社会 support.Methods 有乳癌的 159 个住院病人在哈尔滨医药大学的二所隶属于的医院里正在经历化疗的人,被招募并且调查。每个病人完成了 Herth 希望索引(HHI ) , Jalowiec 处理规模(JCS ) ,和聚会支持小水元做,并且提供了有乳癌的 159 个病人的吝啬的希望水平是的一般人口统计的 data.Results 的规模 38.62erous 为吗?? | ZHANG Jing GAO Wei WANG Ping WU Zhong-hui | 2010 | Chinese Medical Journal2010，，17： | 53 |
| 15 | 2018 Chinese Pediatric Cardiology Society(CPCS) guideline for diagnosis and treatment of syncope in children and adolescents显示文摘Syncope belongs to the transient loss of consciousness(TLOC), characterized by a rapid onset, short duration, and spontaneous complete recovery. It is common in children and adolescents, accounting for 1% to 2% of emergency department visits.Recurrent syncope can seriously affect children's physical and mental health, learning ability and quality of life and sometimes cardiac syncope even poses a risk of sudden death. The present guideline for the diagnosis and treatment of syncope in children and adolescents was developed for guiding a better clinical management of pediatric syncope. Based on the globally recent development and the evidence-based data in China, 2018 Chinese Pediatric Cardiology Society(CPCS) guideline for diagnosis and treatment of syncope in children and adolescents was jointly prepared by the Pediatric Cardiology Society, Chinese Pediatric Society, Chinese Medical Association(CMA)/Committee on Pediatric Syncope, Pediatricians Branch, Chinese Medical Doctor Association(CMDA)/Committee on Pediatric Cardiology, Chinese College of Cardiovascular Physicians, Chinese Medical Doctor Association(CMDA)/Pediatric Cardiology Society, Beijing Pediatric Society, Beijing Medical Association(BMA). The present guideline includes the underlying diseases of syncope in children and adolescents, the diagnostic procedures, methodology and clinical significance of standing test and headup tilt test, the clinical diagnosis vasovagal syncope, postural orthostatic tachycardia syndrome, orthostatic hypotension and orthostatic hypertension, and the treatment of syncope as well as follow-up. | Cheng Wang Yaqi Li Ying Liao Hong Tian Min Huang Xiangyu Dong Lin Shi Jinghui Sun Hongfang Jin Junbao Du Jindou An Jie Chen Mingwu Chen Qi Chen Sun Chen Yonghong Chen Zhi Chen Adolphus Kai-tung Chau Junbao Du Zhongdong Du Junkai Duan Hongyu Duan Xiangyu Dong Lin Feng Lijun Fu Fangqi Gong Yonghao Gui Ling Han Zhenhui Han Bing He Zhixu He Xiufen Hu Yimin Hua Guoying Huang Min Huang Ping Huang Yujuan Huang Hongfang Jin Mei Jin Bo Li Fen Li Tao Li Xiaohui Li Xiaoyan Liu Yan Li Haitao Lv Tiewei Lv Zipu Li Luyi Ma Silin Pan Yusheng Pang Hua Peng Yuming Qin Jie Shen Lin Shi Kun Sun Jinghui Sun Hong Tian Jie Tian Cheng Wang Hong Wang Lei Wang Jinju Wang Wendi Wang Yuli Wang Rongzhou Wu Tianhe Xia Yanyan Xiao Chunhong Xie Yanlin Xing Zhenyu Xiong Baoyuan Xu Yi Xu Hui Yan Shiwei Yang Qijian Yi Xia Yu Xianyi Yu Yue Yuan Hongyan Zhang Huili Zhang Li Zhang Qingyou Zhang Xi Zhang Yanmin Zhang Zhiwei Zhang Cuifen Zhao Bin Zhou Hua Zhu | 2018 | Science Bulletin2018，63，23： | 54 |
| 16 | Immobilization of Lead and Cadmium in Contaminated Soil Using Amendments: A Review显示文摘Since the inception of industrial revolution, metal refining plants using pyrometallurgical processes have generated the prodigious emissions of lead(Pb) and cadmium(Cd). As the core target of such pollutants, a large number of soils are nowadays contaminated over widespread areas, posing a great threat to public health worldwide. Unlike organic pollutants, Pb and Cd do not undergo chemical or microbial breakdown and stay likely in site for longer duration after their release. Immobilization is an in-situ remediation technique that uses cost-effective soil amendments to reduce Pb and Cd availability in the contaminated soils. The Pb and Cd contamination in the soil environment is reviewed with focus on source enrichment, speciation and associated health risks, and immobilization options using various soil amendments. Commonly applied and emerging cost-effective soil amendments for Pb and Cd immobilization include phosphate compounds, liming, animal manure, biosolids, metal oxides, and biochar. These immobilizing agents could reduce the transfer of metal pollutants or residues to food web(plant uptake and leaching to subsurface water) and their long-term sustainability in heavy metal fixation needs further assessment. | Amanullah MAHAR WANG Ping LI Ronghua ZHANG Zengqiang | 2015 | Pedosphere2015，25，4： | 56 |
| 17 | Mobius Isoparametric Hypersurfaces in S^(n+1) with Two Distinct Principal Curvatures | Hai Zhong LI Department of Mathematics, Tsinghua University. Beijing 100084. P. R. China Hui Li LIU Department of Mathematics, Northeastern University. Shenyang 110000. P. R. China Chang Ping WANG Key Laboratory of Pure and Applied Mathematics, School of Mathematical Sciences. Peking University, Beijing 100871, P. R. China Guo Song ZHAO Department of Mathematics, Sichuan University, Chengdu 610064. P. R. China | 2002 | Acta Mathematica Sinica,English Series2002，18，3： | 53 |
| 18 | Inhibitory effects of saikosaponin-d on CCl_4-induced hepatic fibrogenesis in rats显示文摘AIM: To investigate the suppressive effect of saikosaponin-d (SSd) on hepatic fibrosis in rats induced by CCl4 injections in combination with alcohol and high fat, low protein feeding and its relationship with the expression of nuclear factor-κB (NF-κB), tumor necrosis factor-alpha (TNF-α) and interleukins-6 (IL-6). METHODS: Hepatic fibrosis models were induced by subcutaneous injection of CCl4 at a dosage of 3 mL/kg in rats. At the same time, rats in treatment groups were injected intraperitoneally with SSd at different doses (1.0, 1.5 and 2.0 mg/kg) once daily for 6 wk in combination with CCl4, while the control group received olive oil instead of CCl4. At the end of the experiment, rats were anesthetized and killed (except for 8 rats which died during the experiment; 2 from the model group, 3 in high-dose group, 1 in medium-dose group and 2 in low- dose group). Hematoxylin and eosin (HE) staining and Van Gieson staining were used to examine the changes in liver pathology. The levels of alanine aminotransferase (ALT), triglyeride (TG), albumin (ALB), globulin (GLB), hyaluronic acid (HA) and laminin (LN) in serum and the content of hydroxyproline (HYP) in liver were measured by biochemical examinations and radioimmuneoassay, respectively. In addition, the expression of TNF-α and IL-6 in liver homogenate was evaluated by enzyme- linked immunosorbent assay (ELISA) and the levels of NF-κBp65 and I-κBα in liver tissue were analyzed by Western blotting. RESULTS: Both histological examination and Van Gieson staining demonstrated that SSd could attenuate the area and extent of necrosis and reduce the scores of liver fibrosis. Similarly, the levels of ALT, TG, GLB, HA, andLN in serum, and the contents of HYP, TNF-α and IL-6 in liver were all significantly increased in model group in comparison with those in control group. Whereas, the treatment with SSd markedly reduced all the above parameters compared with the model group, especially in the medium group (ALT: 412 ± 94.5 IU/L vs 113.76 ± 14.91 IU/L, TG: 0.95 ± 0.16 mmol/L vs 0.51 ± 0.06 mmol/L, GLB: 35.62 ± 3.28 g/L vs 24.82 ± 2.73 g/L, HA: 42.15 ± 8.25 ng/mL vs 19.83 ± 3.12 ng/mL, LN: 27.56 ± 4.21 ng/mL vs 13.78 ± 2.57 ng/mL, HYP: 27.32 ± 4.32 μg/mg vs 16.20 ± 3.12 μg/mg, TNF-α: 4.38 ± 0.76 ng/L vs 1.94 ± 0.27 ng/L, IL-6: 28.24 ± 6.37 pg/g vs 12.72 ± 5.26 pg/g, respectively, P < 0.01). SSd also decreased ALB in serum (28.49 ± 4.93 g/L vs 37.51 ± 3.17 g/L, P < 0.05). Moreover, the expression of NF-κB p65 in the liver of treated groups was lower than that in model groups while the expression of I-κBα was higher in treated group than in model group (P < 0.01). The expression of NF-κBp65 and TNF-α had a positive correlation with the level of HA in serum of rats after treatment with CCl4 (r = 0.862, P < 0.01; r = 0.928, P < 0.01, respectively). CONCLUSION: SSd attenuates CCl4-induced hepatic fibrosis in rats, which may be related to its effects of hepato-protective and anti-inflammation properties, the down-regulation of liver TNF-α, IL-6 and NF-κBp65 expression and the increased I-κBα activity in liver. | Shuang-Suo Dang Bao-Feng Wang Yan-An Cheng Ping Song Zhen-Guo Liu Zong-Fang Li | 2007 | World Journal of Gastroenterology2007，13，4： | 41 |
| 19 | Validity of Asthma Control Test in Chinese patients显示文摘在中国,背景到目前为止在那里有不是有效或容易的过程定义气喘的控制。这研究估计中国 patients.Methods 的气喘控制测试的有效性三张问询表(气喘控制测试,气喘控制问询表和 30 第二气喘测试) 越过中国从 10 所教学医院予 305 个气喘病人被以。呼吸量测定法也被使用。气喘专家在第一秒根据病人的症状,药和强迫的吐气的体积评估了气喘的控制。病人被划分成 noncontrolled 组并且根据专家的 rating.Reliability,实验有效性和屏蔽精确性控制了组为气喘控制测试分数被进行。屏蔽精确性在 3 张问询表之中被比较。病人的自我等级和专家正在评价也是 5 条款气喘控制测试的内部一致性可靠性是的 compared.Results 0.854。测试和专家正在评估的在气喘控制之间的关联系数是 0.729,它比另外的仪器高。在一些在预言的百分比不同的病人之间区别的气喘控制测试分数在第一秒强迫了吐气的体积(F=26.06, P0.0001 ) ,专家气喘控制的等级(F=88.24, P0.0001 ) 并且气喘控制问询表分数(F=250.57, P0.0001 ) 。气喘控制测试没在正确地分类的百分比与气喘控制问询表显示出重要差别,当百分比正确地分类由时气喘控制测试比 30 第二气喘测试高得多。病人的自我等级与对专家的评价一样(t=0.65, P=0.516 ) 气喘控制测试是的 .Conclusion 为在中国估计气喘控制的一个有效、适用的方法。 | ZHOU Xin DING Feng-ming LIN Jiang-tao YIN Kai-sheng CHEN Ping HE Quan-ying SHEN Hua-hao WAN Huan-ying LIU Chun-tao LI Jing WANG Chang-zheng | 2007 | Chinese Medical Journal2007，，12： | 46 |
| 20 | Berberine reverses free-fatty-acid-induced insulin resistance in 3T3-L1 adipocytes through targeting IKKβ显示文摘AIM: To investigate the effects and molecular mechanisms of berberine on improving insulin resistance induced by free fatty acids (FFAs) in 3T3-L1 adipocytes. METHODS: The model of insulin resistance in 3T3-L1 adipocytes was established by adding palmic acid (0.5 mmol/L) to the culture medium. Berberine treatment was performed at the same time. Glucose uptake rate was determined by the 2-deoxy-[3H]-D-glucose method. The levels of IkB kinase beta (IKKβ) Ser181 phosphorylation, insulin receptor substrate-1(IRS-1) Ser307 phosphorylation, expression of IKKβ, IRS-1, nuclear transcription factor kappaB p65 (NF-κB p65), phosphatidylinositol-3-kinase p85 (PI-3K p85) and glucose transporter 4 (GLUT4) proteins were detected by Western blotting. The distribution of NF-κB p65 proteins inside the adipocytes was observed through confocal laser scanning microscopy (CLSM). RESULTS: After the intervention of palmic acid for 24 h, the insulin-stimulated glucose transport in 3T3-L1 adipocytes was inhibited by 67%. Meanwhile, the expression of IRS-1 and PI-3K p85 protein was reduced, while the levels of IKKβ Ser181 and IRS-1 Ser307 phosphorylation, and nuclear translocation of NF-κB p65 protein were increased. However, the above indexes, which indicated the existence of insulin resistance, were reversed by berberine although the expression of GLUT4, IKKβ and total NF-κB p65 protein were not changed during this study. CONCLUSION: Insulin resistance induced by FFAs in 3T3-L1 adipocytes can be improved by berberine. Berberine reversed free-fatty-acid-induced insulin resistance in 3T3-L1 adipocytes through targeting IKKβ. | Ping Yi Fu-Er Lu Li-Jun Xu Guang Chen Hui Dong Kai-Fu Wang | 2008 | World Journal of Gastroenterology2008，14，6： | 50 |
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