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| 1 | A deep learning-enabled portable imaging flow cytometer for cost-effective, highthroughput, and label-free analysis of natural water samples显示文摘We report a deep learning-enabled field-portable and cost-effective imaging flow cytometer that automatically captures phase-contrast color images of the contents of a continuously flowing water sample at a throughput of 100 mL/h.The device is based on partially coherent lens-free holographic microscopy and acquires the diffraction patterns of flowing micro-objects inside a microfluidic channel.These holographic diffraction patterns are reconstructed in real time using a deep learning-based phase-recovery and image-reconstruction method to produce a color image of each micro-object without the use of external labeling.Motion blur is eliminated by simultaneously illuminating the sample with red,green,and blue light-emitting diodes that are pulsed.Operated by a laptop computer,this portable device measures 15.5 cm×15 cm×12.5 cm,weighs 1 kg,and compared to standard imaging flow cytometers,it provides extreme reductions of cost,size and weight while also providing a high volumetric throughput over a large object size range.We demonstrated the capabilities of this device by measuring ocean samples at the Los Angeles coastline and obtaining images of its micro-and nanoplankton composition.Furthermore,we measured the concentration of a potentially toxic alga(Pseudo-nitzschia)in six public beaches in Los Angeles and achieved good agreement with measurements conducted by the California Department of Public Health.The cost-effectiveness,compactness,and simplicity of this computational platform might lead to the creation of a network of imaging flow cytometers for largescale and continuous monitoring of the ocean microbiome,including its plankton composition. | Zoltán Gӧrӧcs Miu Tamamitsu Vittorio Bianco Patrick Wolf Shounak Roy Koyoshi Shindo Kyrollos Yanny Yichen Wu Hatice Ceylan Koydemir Yair Rivenson Aydogan Ozcan | 2018 | Light(Science & Applications)2018，7，1： | 14 |
| 2 | Subcycle control of terahertz waveform polarization using all-optically induced transient metamaterials显示文摘Coherent radiation with frequencies ranging from 0.3 to 30 THz has recently become accessible using femtosecond laser technology.These terahertz(THz)waves have already been applied in spectroscopy and imaging and can be manipulated using static optical elements such as lenses,polarizers and filters.However,ultrafast modulation of THz radiation is required as well,for instance,in short-range wireless communication or for preparing shaped THz transients for the coherent control of numerous material excitations.Here,we demonstrate an all-optically created transient metamaterial that allows us to manipulate the polarization of THz waveforms with subcycle switch-on times.The polarization-modulated pulses are potentially interesting for controlling elementary motions such as the vibration of crystal lattices,the rotation of molecules and the precession of spins. | N Kamaraju Andrea Rubano Linke Jian Surajit Saha T Venkatesan Jan Notzold R Kramer Campen Martin Wolf Tobias Kampfrath | 2014 | Light(Science & Applications)2014，3，1： | 5 |
| 3 | Three-dimensional analysis of the physiological foramen geometry of maxillary and mandibular molars by means of micro-CT显示文摘The aim of this study was to investigate the physiological foramen diameter, shape and distance between physiological and anatomical apex of maxillary and mandibular first and second molars. Accurate knowledge of the physiological foramina morphology; thus, inherent mechanical shaping technical hindrances, is decisive when taking the corresponding root canal final preparation decision. The morphological dimensions of a total of 1 727 physiological foramina were investigated by means of micro-computed tomography. Mean narrow and wide(to a high number, oval) diameters of the physiological foramen were 0.24,0.22 and 0.33 mm and 0.33, 0.31 and 0.42 mm in mesiobuccal(MB), distobuccal(DB) and palatal(P) roots in maxillary first molars; 0.24, 0.22 and 0.33 mm and 0.41, 0.33 and 0.44 in MB, DB, and P roots in maxillary second molars. Mandibular first molars showed mean narrow and wide diameters of 0.24 and 0.30 mm and of 0.39 and 0.46 mm in mesial(M) and distal(D)roots; second mandibular molars showed 0.25 and 0.31 mm and 0.47 mm in M and D roots. The mean distance between the physiological foramina and anatomical apex was 0.82, 0.81 and 1.02 mm and 0.54, 0.43 and 0.63 mm in MB, DB and P roots of the maxillary first and second molars, respectively. A mean distance of 0.95 mm(M) and 1.05 mm(D) in the first and0.78 mm(M) and 0.81 mm(D) in the second mandibular molars was observed. Based on the results obtained, assumable recommendations for final preparation size of the physiological foramen were calculated. However, when taking into consideration, the resulting standard deviations of marginal errors must be cautiously considered when taking a final decision in clinical endodontic treatment. | Thomas Gerhard Wolf Frank Paqué Michael Sven Patyna Brita Willershausen Benjamín Briseno-Marroquín | 2017 | International Journal of Oral Science2017，9，3： | 4 |
| 4 | Root canal morphology and configuration of 123 maxillary second molars by means of micro-CT显示文摘The aim of this study was to investigate the root canal configuration, accessory canals and number of main foramina of 123 maxillary second molars by means of micro-computed tomography. The teeth were scanned and reproduced with 3D software imaging. The root canal configuration and number of main foramina were evaluated by means of a four-digit system. The morphological complexity of human maxillary second molars is depicted by the number of accessory and connecting canals. The most frequently observed root canal configurations in the mesiobuccal root were 2-2-2/2(19.5%), 2-2-1/1(14.6%) and 2-1-1/1(13.0%). A 1-1-1/1 configuration was observed in 93.5% and in 96.7% in the distobuccal and palatal roots, respectively. The MB1 root canal had one accessory canal(18.7%), and 8.9% of the MB2 root canal had one or two accessory canals. The distobuccal(11.3%) and palatal(14.6%) root canals had at least one accessory canal, and connecting canals were observed in16.3% of mesiobuccal roots. The MB1, MB2, distobuccal and palatal root canals had one main foramen in 99.2%, 43.1%,98.4% and 99.2% of samples, respectively. In the mesiobuccal root, one accessory foramen was detected in 14.6%, two were detected in 7.3%, and three were detected in 5.7%. The distobuccal root showed one or two accessory foramina in 9.1% of samples. The root canal configuration of maxillary second molars is quite heterogeneous; the mesiobuccal root has predominantly two root canal entrances(58.4%, 1 in 41.1%) with one main foramen(54.4%). Two main foramina were observed in 43.0%. Morphological variations, connecting and accessory canals were observed in all apical thirds. | Thomas Gerhard Wolf Frank Paqué Anja-Christin Woop Brita Willershausen Benjamín Briseno-Marroquín | 2017 | International Journal of Oral Science2017，9，1： | 3 |
| 5 | A Novel Line Immunoassay Based on Recombinant Virulence Factors Enables Highly Specific and Sensitive Serologic Diagnosis of Helicobacter pylori Infection显示文摘 | Luca Formichella Laura Romberg Christian Bolz Michael Vieth Michael Geppert Gereon G?ttner Christina N?lting Dirk Walter Wolfgang Schepp Arne Schneider Kurt Ulm Petra Wolf Dirk H. Busch Erwin Soutschek Markus Gerhard | 2013 | Clinical and Vaccine Immunology2013，，11： | 2 |
| 6 | A Short Amino-Terminal Part of Arabidopsis Phytochrome A Induces Constitutive Photomorphogenic Response显示文摘Phytochrome A (phyA ) 是在 Arabidopsis thaliana 察觉到的 far-red 光的主导的光敏电阻器。phyA 在发信号的变白的幼苗,和导致光的 phyA 的细胞质在高水平积累被一个复杂规章的网络调停。这包括光 -- 并且进在 vivo 的原子核的本国的 phyA 的 FHY1/FHL 蛋白质依赖者 translocation。phyA (PHYA406 ) 的短 N 终端碎片对 phenocopy 足够,这也被显示出在 vitro 的这个高度调整的细胞的过程。为了测试这 N 终端的生物活动,在 planta phyA 碎裂,我们生产了表示 PHYA406YFP 的转基因的 phyA-201 植物(黄荧光灯蛋白质) DD, PHYA406YFPDDNLS (原子本地化信号) ,和 PHYA406YFPDDNES (原子出口信号) 熔化蛋白质。这里,我们报导 PHYA406YFPDD 被进口进原子核,而 PHYA406YFPDDNLS 和 PHYA406YFPDDNES 显示期望的组成的本地化模式,这进程部分是轻依赖者的。我们的结果证明这些截断的 phyA 蛋白质是轻马厩的,当局部性时,他们在他们的原子核,和两个都不触发组成的象 photomorphogenic 一样回答导致合适的 phyA 发信号。我们证明在 vitro 并且在 vivo PHYA406, Pfr 和 Pr 绑 COP1, photomorphogenesis 的一个一般抑压者,并且在原子身体与它共同本地化。因此,我们断定在 planta,截断的 PHYA406 蛋白质以一种光无关的方式在原子核使 COP1 失去活性。 | Andra's Viczia'n E'va Ada'm Iris Wolf Ja'nos Bindics Stefan Kircher Marc Heijde Roman Ulm Eberhard Scha'fer Ferenc Nagy | 2012 | Molecular Plant2012，5，3： | 2 |
| 7 | Structural acoustics finite element analysis of the aoutomobile passenger compartment: A review of current practice显示文摘 | Nefske N J Wolf J A Jr Howell L J | 1982 | Journal of Sound Vibration1982，80，2： | 1 |
| 8 | Muhiresidue determination of sulfonylurea herbicides by capillary electrophoresis for hydrolysis studies in water and sediments 显示文摘 | Berger B M Lee Wolfe N | 1996 | Fresenius'' Journal of Analytical Chemistry1996，356，8： | 1 |
| 9 | Survey ofwater-extractable phosphorus in livestock manures显示文摘 | KLElNMAN P J A WOLF A M SHARPLEY A N | 2005 | Soil ScienceSociety of America Journal2005，69，3： | 1 |
| 10 | Improving metabolic con- trol reverses the histomorphometric and biomechanical ab- normalities of an experimentally induced bone defect in spontaneously diabetic rats 显示文摘 | Follak N K1/ting I Wolf E | 2004 | Calcif Tissue Int2004，74，6： | 1 |
| 11 | The elusive memristor: properties of basic electrical circuits 显示文摘 | Joglekar Y N Wolf S J | 2009 | European Journal of Physics2009，30，4： | 1 |
| 12 | The elusive memristor: properties of basic electrical circuits显示文摘 | JOGLEKAR Y N WOLF S J | 2008 | European Journal of Physics2008，30，4： | 1 |
| 13 | Acoustic propagation through an internal wave field in a shallow waveguide显示文摘 | Tielbuerger D Finette S Wolf S N | 1997 | J Acoust Soc Am1997，101，： | 1 |
| 14 | Diffuse cortical necrosis in a neonate with incontinentia piginenti and an encephalitis - like presentation 显示文摘 | Wolf NI Kramer N Harting I | 2005 | AJNR2005，26，： | 1 |
| 15 | Plasmid CpG depletion improves degree and duration of tumor gene ex- pression after intravenous administration of polyplexes 显示文摘 | de Wolf HK Johansson N Thong AT | 2008 | PharmRes2008，25，7： | 1 |
| 16 | Identification of ZNF313 / RNF114 as a novel psoriasis susceptibility gene显示文摘 | Capon F Bijlmakers MJ Wolf N | 2008 | Hum Mol Genet2008，17，： | 1 |
| 17 | Ammonia-oxidizing bacteria in a chloraminated distribution system: seasonal occurrence, distribution and disinfection resistance显示文摘 | WOLFE R L LIEU N I IZAGUIRRE G | 1990 | Appl Environ Microbiol1990，56，2： | 1 |
| 18 | On the degradation of methyl bromide in seawater显示文摘 | JEFFERS P M WOLFE N L | 1996 | Geophysical Research Letter1996，23，： | 1 |
| 19 | CO2 enhanced coalbed methane production in the Netherlands显示文摘 | Hamelinck C N Faaij A P C Turkenburg W C F van Bergen Pagnier H J M Barzandji O H M Wolf K -H A A Ruijg G J | 2002 | Energy2002，27，7： | 1 |
| 20 | Kinematics of the scaphoid shift test显示文摘 | Wolfe SW Haven N Gupta A | 1997 | J Hand Surg(Am)1997，22，： | 1 |
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