|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Intelligent transportation systems for smart cities:a progress review显示文摘To better meet the challenge of providing effective,low-cost,energy efficient transport services,the concept of intelligent transport systems(ITS) has been proposed and lauded as an innovative and promising solution for next generation transport networks.In this paper,the progress of ITS research around the world is briefly reviewed and current challenges are outlined,thereby offering further insight into ITS development for all researchers in this area. | XIONG Zhang SHENG Hao RONG WenGe COOPER Dave E | 2012 | Science China(Information Sciences)2012，55，12： | 16 |
| 2 | A Simplified PCR-SSP Method for HLA-A2 Subtype in a Population of Wuhan,China显示文摘HLA-A2 is the most frequent HLA-A allele in all ethnic populations, and an important restriction element for peptide presentation to T cells in infectious disease and cancer. However, the HLA-A2 supertype consisting of up to 75 subtypes, mutation studies and analyses using cytotoxic T lymphocytes suggest the functional relevance of subtype-specific differences in HLA-A2 molecules for peptide binding and T-cell recognition. Therefore, it is necessary for T-cell response study to discriminate the HLA-A2 subtypes and to understand the profile of HLA-A2 allelic distribution in a given population. In this study, we developed a simple, robust approach based on the nested polymerase chain reaction using sequence-specific primers (PCR-SSP) to discriminate 17 HLA-A2 subtypes which cover the most HLA-A2 alleles (> 99% allele frequency) reported in Chinese, using 15 combinations of 19 allelic specific primers. In the first round of PCR, 3 combinations of 5 primers were used to determine whether the tested sample was HLA-A2 positive, meanwhile the subtypes of HLA-A*0209 and HLA-A*0215N were determined for the variant position of these two subtypes is in exon 4 instead of exon 2, 3. Samples of HLA-A2 positive were subtyped in the second round of PCR, using PCR products of the first round as templates. This strategy was applied to test the samples of 78 random HLA-A2 positive individuals for their HLA-A2 subtypes. Those samples were screened for HLA-A2 positive by the first round PCR-SSP from 154 healthy blood donors in Wuhan, China. The subtyping results were verified by using flow cytometric analysis (FCM) with HLA-A2 specific monoclonal antibody BB7.2 and DNA sequencing. The typing results of the samples show 50.7% random individuals in the population carry HLA-A2, HLA-A*0201 ranks the first (allele frequency = 15.5%), followed by A*0207 (5.8%), A*0206 (4.7%), A*0203 (2.6%), A*0210 (0.7%), and these 5 alleles account for 99.0% HLA-A2 subtypes of allele frequency. Our study indicates that the developed typing method is simple and reliable for HLA-A2 subtyping in Chinese, and the profile of allelic distribution of HLA-A2 subtypes is revealed in the population of Wuhan, China. | Bing Liang Lijun Zhu Zhihui Liang Xiufang Weng Xiaoling Lu Cai'e Zhang Hui Li Xiongwen Wu | 2006 | Cellular & Molecular Immunology2006，3，6： | 9 |
| 3 | Evidence That the Diabetes Gene Encodes the Leptin Receptor: Identification of a Mutation in the Leptin Receptor Gene in db / db Mice显示文摘 | Hong Chen Olga Charlat Louis A Tartaglia Elizabeth A Woolf Xun Weng Stephen J Ellis Nathan D Lakey Janice Culpepper Karen J More Roger E Breitbart Geoffrey M Duyk Robert I Tepper Jay P Morgenstern | 1996 | Cell1996，，3： | 2 |
| 4 | The prevalence and prognostic value of BRAF mutation in thyroid cancer显示文摘 | Kebebew E Weng J Bauer J | 2007 | Ann Surg2007，246，3： | 1 |
| 5 | Complexation with dissolved organic matter and solubility control of heavy metals in a sandy soil 显示文摘 | WENG L P TEMMINGHOFF E J M LOFTS S | 2002 | Environ Sci Technol2002，36，： | 1 |
| 6 | A parts selection model with one-way substitution显示文摘 | Chand S Ward J E Weng Z K | 1994 | European Journal of Operational Research1994，73，11： | 1 |
| 7 | Evaluation of interface shear strength and residual stress of sol-gel derived fluoridated hydroxyapatite coatings on Ti6A14V substrates显示文摘 | ZHANG S WANG Y S ZENG X T CHENG K QIAN M SUN D E WENG W J CHIA W Y | | 0，，： | 1 |
| 8 | Synthesis of Long- Chain Branched Propylene Polymers via Macromonomer In- corporation 显示文摘 | Weng W Markel E J Dekmezian A H | 2001 | MacromolRapid Commun2001，22，18： | 1 |
| 9 | Effects of Humic acid and competing cations on metal absorption by Lolium perenne 显示文摘 | Kalis E J J Temminghoff E J M Weng L | 2005 | Environmental Toxicology and Chemis- try2005，25，3： | 1 |
| 10 | The prevalence and prognostic value of BRAF mutation in thyroid cancer显示文摘 | Kebebew E Weng J Bauer J | 2007 | Ann Surg2007，246，3： | 1 |
| 11 | Higher-Order Expansions of Distributions of Maxima in a Hiisler-Reiss Model 显示文摘 | HASHORVA E PENG Zuo-xiang WENG Zhi-chao | 2014 | Methodology and Computing in Applied Probability2014，18，1： | 1 |
| 12 | New ECG enhancement algorithm for stress ECG tests显示文摘 | Blanco M Weng B Bamer K E | 2006 | Computers in Cardiology2006，33，： | 1 |
| 13 | Complexation with disso lved organic matter and solubility control of heavy metals in a sandy soil显示文摘 | WENG L TEMMINGHOFF E J LOFTS S | 2002 | Environmental Science & Technology2002，36，22： | 1 |
| 14 | Modeling distribution changes of vegetation in China under future climate change显示文摘 | Weng E S Zhou G S | 2006 | Environment Modeling and Assessment2006，11，： | 1 |
| 15 | ECG sig- nal denoising and baseline wander correction based on the empirical mode decomposition 显示文摘 | MANUEL B V WENG B W BARNER K E | 2008 | Computers in Biology and Medicine2008，38，1： | 1 |
| 16 | ECG signal denoising and baseline wander correction based on the empirical mode decomposition显示文摘 | Blanco-Velasco M Weng B Barner K E | 2008 | Computers in Biology and Medicine2008，38，1： | 1 |
| 17 | Accuracy and clinicalimpact of mediastinal lymph node staging with FDG-PETimaging in potentially resectable lung cancer显示文摘 | Weng E Tran L Rege S | 2000 | Am J ClinOncol2000，23，1： | 1 |
| 18 | Calcium ionophore-treated myeloid cells acquire many dendritic cell characteristics independent of prior differentiation state,transformation status,or sensitivity to biologic agents显示文摘 | Koski G K Schwartz G N Weng D E | 1999 | Blood1999，94，4： | 1 |
| 19 | Accuracy and clinical impact of mediastinal lymph node staging with FDG-PET imaging in potentially resctable lung cancer显示文摘 | Weng E Tran L Rege S | 2000 | Am J Clin Oncol2000，23，1： | 1 |
| 20 | The blood-brain barrier: structure, function and therapeutic approa- ches to cross it显示文摘 | TAES M RAMOS-FERNANDEZ E WENG JX | 2014 | Mol Membr Biol2014，31，5： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员