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| 1 | Transgenic rice expressing a novel phytase-lactoferricin fusion gene to improve phosphorus availability and antibacterial activity显示文摘The developing trends of livestock production are efficiency,safety and sustainability,which face two major challenges:low availability of phytate phosphorus and abuse of antibiotics.As a solution phytases and antimicrobial peptides are applied as feed additives.However,phytases and antimicrobial peptides are susceptible to proteases,costly by fermentation and potential toxic to production hosts.We transformed an optimized phytase-lactoferricin fusion gene PhyLf driven by an endosperm-specific promoter Gt13aP and Bar(bialaphos resistance) gene as a selection maker into rice.The Bar and PhyLf genes were integrated into the rice genome,stably inherited and expressed.Their phosphinothricin acetyl transferase(PAT)protein content oftransgenic plants with glufosinate resistance varied between 50.45-93.39 ugg^(-1).Fusion protein expressed especially in the seeds oftransgenic rice had a summit phytase activity at 32.30Ug^(-1) which increased by 61.71-fold compared to the control/check group(CK) and 7.54-fold compared to un-optimized transgenic plant.The highest inorganic phosphorus(Pi) content of the transgenic seeds reached 13.15 mg g^(-1) increased by 12.77-fold compared to that of CK.Preliminary antibacterial experiments showed that the enterokinase hydrolysate product of fusion protein could inhibit the growth of Escherichia coli DH5a.These results indicated that the protein PhyLf has the potential to increase availability of feed phytate phosphorus,improve consumer's immunity and reduce the use of antibiotics. | WANG Zuo-ping DENG Li-hua WENG Lü-shui DENG Xiang-yang FU Xi-qin XIN Ye-yun XIAO Guo-ying | 2017 | Journal of Integrative Agriculture2017，16，4： | 4 |
| 2 | Effect of herb-partitioned moxibustion on dopamine levels and dopamine receptor 1 expression in the colon and central nervous system in rats with Crohn’s disease显示文摘OBJECTIVE:To observe the effect of herb-partitioned moxibustion at the Tianshu (ST 25) and Qihai (CV 6) acupoints in rats with Crohn's disease,and explore the underlying mechanism from dopamine (DA) and dopamine receptor 1 (D1 R) in the colon,spinal dorsal horn and hypothalamus.METHODS:The rats were randomly divided into the normal,model (CD),herb-partitioned moxibustion (Mox) and mesalazine (Mesa) groups.Damage in the colons was scored and observed by hematoxylin and eosin staining.DA and D1R protein expression in the colonic mucosa were detected by immunohistochemistry.The concentrations of DA and D1R in the spinal dorsal horn and hypothalamus were measured by enzyme-linked immunosorbent assay,and D1R mRNA expression was evaluated by quantitative real-time polymerase chain reaction.RESULTS:In the colon,compared with the normal group,DA,D1 R protein expressions and D1 R mRNA expression were significantly higher in the model group,while decreased in the Mox group and the Mesa group.In the spinal dorsal horn and hypothalamus,compared with the normal group,the concentrations of DA and D1 R,and the D1R mRNA expressions were significantly higher in the model group,and decreased in the Mox group and the Mesa group.CONCLUSION:Herb-partitioned moxibustion at the Tianshu (ST 25) and Qihai (CV 6) acupoints relieved ulceration in CD rats,the underlying mechanism maybe relative with the regulation of DA and D1R in the colon,spinal dorsal horn and hypothalamus by moxibustion. | Lu Yuan Zheng Handan Lü Tingting Ma Zhe Wu Huangan Xu Manwen Weng Zhijun Feng Hui Zhang Fang Ding Guanghong Wu Luyi Liu Huirong | 2019 | Journal of Traditional Chinese Medicine2019，39，3： | 3 |
| 3 | Fixed point iteration for local strictly pseudocontractive mapping显示文摘 | Weng X L | 1991 | Pro Amer Math Soc1991，113，： | 2 |
| 4 | Comparative studies on the hypolipidemic and growth suppressive effects of oolong, black, pu-erh, and green tea leaves in rats显示文摘 | Kuo K L Weng M S Chiang C T | 2005 | Journal of Agricultural and Food Chemistry2005，53，2： | 1 |
| 5 | Immobilizationof RGD peptide on HA coating through a chemical bondingapproach显示文摘 | YANG C L CHENG K WENG W J | 2009 | Journal of Materials Science:Materials in Medi-cine2009，20，11： | 1 |
| 6 | Effects of additives on micro-structures and microwave dielectric properties of (Zr,Sn)TiO4 ceramics显示文摘 | Huang C L Weng M H Chen H L | 2001 | Mater Chem Phys2001，71，： | 1 |
| 7 | Effects of addtives on microstructure and microwave dielectric properties of (Zr,Sn) TiO4 ceramics显示文摘 | HUANG C L WENG M H CHEN H L | 2001 | Mater Chem Phys2001，71，： | 1 |
| 8 | High-strength reinforced concrete deep and short beams:shear design equations in North America and UK practice显示文摘 | Tan K H Dong F K Weng L W | 1998 | ACI Structural Journal1998，95，3： | 1 |
| 9 | Anti-inflammatory flavonoids from A rtocarpus heterophyllus and Artocarpus communis显示文摘 | Wei B L Weng J R Chiu P H | 2005 | J Agr Food Chem2005，53，10： | 1 |
| 10 | Guangxi Liuzhou AIDS epidemic characteristics and trend analysis显示文摘 | Feng WD Weng YQ Wei L | 2006 | Public Health and Preventive Medicine2006，，05： | 1 |
| 11 | Morphology and mechanical properties of intermetallic compounds in SnAgCu solderjoints显示文摘 | Chandra Rao B S S Weng J Shen L | 2010 | MicroelectronEngineering2010，87，11： | 1 |
| 12 | PTEN induces apoptosis and cell cycle arrest through phosphoinosistol - 3 - kinase/Akt - dependent and independent pathways 显示文摘 | Weng L Brown JL Eng C | 2001 | Hum Mol Gent2001，10，3： | 1 |
| 13 | Migration of Cu species in Cu/SAPO-34 during hydrothermal aging显示文摘 | WANG L GAUDET J R WEI L WENG D | 2013 | J Catal2013，306，： | 1 |
| 14 | Identification and expression cloning of a leptin receptor, OB - R 显示文摘 | TARTAGLIA L A DEMBSKI M WENG X | 1995 | Cell1995，83，7： | 1 |
| 15 | Antiviral effect of epigallocatechin gallate on enterovirus 71显示文摘 | Ho H Y Cheng M L Weng S F | | 0，，14： | 1 |
| 16 | Estimatingnumber of transgene copies in transgenic rapeseed by realtime PCR assay with HMG I/Y as an endogenous reference gene显示文摘 | Weng H B Pan A H Yang L T | 2004 | Plant Molecular Biology Reporter2004，22，： | 1 |
| 17 | Solvothermal synthesis andvisible/infrared optical properties of Al/Fe 3 0 4 core-shellmagnetic composite pigments 显示文摘 | Yuan L Weng X Xie J | 2013 | Journal of Alloys &Compounds2013，580，32： | 1 |
| 18 | Transcutaneous oxygen pressure measurement in diabetic foot ulcers: mean va- lues and cut-point for wound healing显示文摘 | Yang C Weng H Chen L | 2013 | J Wound Osto- my Continence Nurs2013，40，6： | 1 |
| 19 | An evaluation approach to engineering design in QFD processes using fuzzy goal programming models显示文摘 | CHEN L H WENG M C | 2006 | European Journal of Operational Research2006，172，1： | 1 |
| 20 | Microstructure and mechanical properties of bulk Mg-Zn-Ca amorphous alloys and amorphous matrix composites显示文摘 | LI Q F WENG H R SUO Z Y REN Y L YUAN X G QIU K Q | 2008 | Mater Sci Eng A2008，487，： | 1 |
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