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| 1 | Luciferase reporter phage phAE85 for rapid detection of rifampicin resistance in clinical isolates of Mycobacterium tuberculosis显示文摘Objective:To evaluate luciferase reporter phage(LRP)phAE85 in rapid detection of rifampicin resistance in a region where TB is endemic.Methods:One hundred and ninety primary isolates on Lowenstein-Jensen medium were tested.Middlebrook 7H9 complete medium with and without rifampicin at 2μg/mL was inoculated with standard inoculum from suspensions of the clinical isolate.After incubation for 72 h,LRP was added.Following 4 h of further incubation,light output from both control and test was measured as relative light units.Strains exhibiting a reduction of less than 50%relative light units in the drug containing vial compared to control were classified as resistant.Results were compared with the conventional minimum inhibitory concentration method(MIC)of drug susceptibility testing.Results:The two methods showed high level of agreement of 97%(CI 0.94,0.99)and P value was 0.000 1.The sensitivity and specificity of LRP assay for detection of rifampicin resistance were 91%h(CI 0.75,0.98)and 99ct(CI0.95,1.00)respectively.Time to detection of resistance by LRP assay was 3 d in comparison with 28 d by the minimum inhibitory concentration method.Conclusions:LRP assay with phAE85 is 99%specific,91%sensitive and is highly reproducible.Thus the assay offers a simple procedure for drug sensitivity testing,within die scope of semi-automation. | Gomathi Sivaramakrishnan Balaji Subramanyam Ponnuraja C Vanaja Kumar | 2013 | Asian Pacific Journal of Tropical Medicine2013，6，9： | 4 |
| 2 | Potential applications of lactic acid bacteria and bacteriocins in antimycobacterial therapy显示文摘Tuberculosis(TB) is a communicable disease caused by Mycobacterium tuberculosis(M. tuberculosis). WHO estimated that 10.4 million new(incident) TB cases worldwide in year 2016. The increased prevalence of drug resistant strains and side effects associated with the current anti-tubercular drugs make the treatment options more complicated. Hence, there are necessities to identify new drug candidates to fight against various sub-populations of M. tuberculosis with less or no toxicity/side effects and shorter treatment duration. Bacteriocins produced by lactic acid bacteria(LAB) attract attention of researchers because of its 'Generally recognized as safe' status. LAB and its bacteriocins possess an effective antimicrobial activity against various bacteria and fungi. Interestingly bacteriocins such as nisin and lacticin 3147 have shown antimycobacterial activity in vitro. As probiotics, LAB plays a vital role in promoting various health benefits including ability to modulate immune response against various infectious diseases. LAB and its metabolic products activate immune system and thereby limiting the M. tuberculosis pathogenesis. The protein and peptide engineering techniques paved the ways to obtain hybrid bacteriocin derivatives from the known peptide sequence of existing bacteriocin. In this review, we focus on the antimycobacterial property and immunomodulatory role of LAB and its metabolic products. Techniques for large scale synthesis of potential bacteriocin with multifunctional activity and enhanced stability are also discussed. | Anbarasu Sivaraj Revathy Sundar Radhakrishnan Manikkam Krupakar Parthasarathy Uma Rani Vanaja Kumar | 2018 | Asian Pacific Journal of Tropical Medicine2018，11，8： | 2 |
| 3 | Synthesis,antimyeobaeterial activity evaluation, and QSAR studies of chalcone derivatives 显示文摘 | P M Sivakumar S Prabu Seenivasan Vanaja Kumar Mukesh Doble | 2007 | Bioorganic & Medicinal Chemistry Letters2007，17，6： | 1 |
| 4 | Synthesis,antimycobacterial activity evaluation,and QSAR studies of chalcone derivatives显示文摘 | SIVAKUMAR P M PRABU SEENIVASAN S VANAJA KUMAR | 2007 | Bioorganic & Medicinal Chemistry Letters2007，17，： | 1 |
| 5 | Mukesh doble experimental and theoretical approaches to enhance anti tubercular activity of chalcones 显示文摘 | Sivakumar P M Vanaja Kumar Prabu Seenivasan S | 2010 | Wseas Transactions on Biology and Biomedicine2010，7，2： | 1 |
| 6 | Functioning of olivocochlear bundle and speech perception in noise显示文摘 | Kumar U A Vanaja C S | | 0，，02： | 1 |
| 7 | Phage lysin to supplement phagebiotics to decontaminate processed sputum specimens显示文摘 | Balaji Subramanyam Vanaja Kumar Venkatesan Perumal Selvakumar Nagamiah | 2010 | European Journal of Clinical Microbiology & Infectious Diseases2010，，11： | 1 |
| 8 | An alternative sputum processing method using chitin for the isolation of Mycobacterium tuberculosis显示文摘 | Balaji Subramanyam Azger Dusthackeer Fathima Rehman Gomathi Sekar Gomathi N. Sivaramakrishnan Vanaja Kumar | 2010 | World Journal of Microbiology and Biotechnology2010，，3： | 1 |
| 9 | Phage cocktail to control the exponential growth of normal flora in processed sputum specimens grown overnight in liquid medium for rapid TB diagnosis显示文摘 | Vanaja Kumar S. Balaji N.S. Gomathi P. Venkatesan Gomathi Sekar K. Jayasankar P.R. Narayanan | 2006 | Journal of Microbiological Methods2006，，3： | 1 |
| 10 | Preliminary screening for antibacterial and antimycobacterial activity of actinomycetes from less explored ecosystems显示文摘 | Manikkam Radhakrishnan S. Suganya Ramasamy Balagurunathan Vanaja Kumar | 2010 | World Journal of Microbiology and Biotechnology2010，，3： | 1 |
| 11 | Effect of bacteriophage lysin on lysogens显示文摘Objective:To study the effect of phage lysin on the growth of lysogens.Methods:Sputum specimens processed by modified Petroff's method were respectively treated with phagebiotics in combination with lysin and lysin alone.The specimens were incubated at 37 ℃ for 4 days.At the end of day 1,2,3 and day 4,the specimens were streaked on blood agar plates and incubated at37 ℃ for 18-24 hours.The growth of normal flora observed after day 1 was considered as lysogens.Results:Sputum specimens treated with phagebiotics-lysin showed the growth of lysogens.When specimens treated with lysin alone,lysogen formation was avoided and normal flora was controlled.Conclusions:Lysin may have no effect on the growth of lysogens. | Balaji Subramanyam Vanaja Kumar | 2011 | Asian Pacific Journal of Tropical Biomedicine2011，1，4： | 0 |
| 12 | X-linked deafness: A review of clinical and radiological findings and current management strategies显示文摘X-linked deafness is a rare genetic disorder causing a severe mixed hearing loss. This is due to an abnormal connection between the internal auditory meatus(IAM) and the basal turn of the cochlear leading to a '3rd window effect' and cochlear conductive hearing loss. Patients are traditionally treated with conventionalhearing aids however these are often unsatisfactory. Cochlear implantation is a high-risk procedure in such cases due to the risk of inadvertent electrode placement in the IAM. We present three paediatric cases where the hearing loss was managed with a combination of a bone anchored hearing aid in combination with a conventional behind the ear hearing aid. We also present a review of the current literature regarding the management of X-linked deafness. | Sonia Kumar Thomas Mawby Vanaja Sivapathasingam Jane Humphries James Ramsden | 2016 | World Journal of Otorhinolaryngology2016，6，1： | 0 |
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