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| 1 | Treatment of metastatic colorectal carcinomas by systemic inhibition of vascular endothelial growth factor signaling in mice显示文摘AIM: Tumor angiogenesis has been shown to be promoted by vascular endothelial growth factor (VEGF) via stimulating endothelial cell proliferation, migration, and survival. Blockade of VEGF signaling by different means has been demonstrated to result in reduced tumor growth and suppression of tumor angiogenesis in distinct tumor entities. Here, we tested a recombinant adenovirus, AdsFIt1-3, that encodes an antagonistically acting fragment of the VEGF receptor 1 (Flt-1), for systemic antitumor effects in pre-established subcutaneous CRC tumors in mice. METHODS: Murine colorectal carcinoma cells (CT26) were inoculated subcutaneously into Balb/c mice for in vivo studies. Tumor size and survival were determined. 293 cell line was used for propagation of the adenoviral vectors. Human lung cancer line A549 and human umbilical vein endothelial cells were transfected for in vitro experiments. RESULTS: Infection of tumor cells with AdsFlt1-3 resulted in protein secretion into cell supernatant, demonstrating correct vector function. As expected, the secreted sFlt1-3 protein had no direct effect on CT26 tumor cell proliferation in vitro, but endothelial cell function was inhibited by about 46% as compared to the AdLacZ control in a tube formation assay. When AdsFlt1-3 (5×109 PFU/animal) was applied to tumor bearing mice, we found a tumor inhibition by 72% at d 12 after treatment initiation. In spite of these antitumoral effects, the survival time was not improved. According to reduced intratumoral microvessel density in AdsFIt1-3-treated mice, the antitumor mechanism can be attributed to angiostatic vector effects. We did not detect increased systemic VEGF levels after AdsFlt1-3 treatment and liver toxicity was low as judged by serum alanine aminotransferase determination. CONCLUSION: In this study we confirmed the value of a systemic administration of AdsFIt1-3 to block VEGF signaling as antitumor therapy in an experimental metastatic colorectal carcinoma model in mice. | Volker Schmitz Miroslaw Kornek Tobias Hilbert Christian Dzienisowicz Esbher Raskopf Christian Rabe Tilman Sauerbruch Cheng Qian Wolfgang H Caselmann | 2005 | World Journal of Gastroenterology2005，11，28： | 4 |
| 2 | Heparin-induced thrombocytopenia in solid organ transplant recipients: The current scientific knowledge显示文摘Exposure to heparin is associated with a high incidence of immunization against platelet factor 4(PF4)/heparin complexes. A subgroup of immunized patients is at risk of developing heparin-induced thrombocytopenia(HIT), an immune mediated prothrombotic adverse drug effect. Transplant recipients are frequently exposed to heparin either due to the underlying end-stage disease, which leads to listing and transplantation or during the transplant procedure and the perioperative period. To review the current scientific knowledge on antiheparin/PF4 antibodies and HIT in transplant recipients a systematic Pub Med literature search on articles in English language was performed. The definition of HIT is inconsistent amongst the publications. Overall, six studies and 15 case reports have been published on HIT before or after heart, liver, kidney, and lung transplantation, respectively. The frequency of seroconversion for anti-PF4/heparin antibodies ranged between 1.9% and 57.9%. However, different methods to detect anti-PF4/heparin antibodies were applied. In none of the studies HIT-associated thromboembolic events or fatalities were observed. More importantly, in patients with a history of HIT, reexposure to heparin during transplantation was not associated with thrombotic complications. Taken together, the overall incidence of HIT after solid organ transplantation seems to be very low. However, according to the current knowledge, cardiac transplant recipients may have the highest risk to develop HIT. Different alternative suggestions for heparin-free anticoagulation have been reported for recipients with suspected HIT albeit no official recommendations on management have been published for this special collective so far. | Volker Assfalg Norbert Hüser | 2016 | World Journal of Transplantation2016，6，1： | 2 |
| 3 | Enucleation and Limited Pancreatic Resection Provide Long-Term Cure for Insulinoma in Multiple Endocrine Neoplasia Type 1显示文摘 | Bartsch Detlef K Albers Max Knoop Richard Kann Peter H Fendrich Volker Waldmann Jens | 2014 | Neuroendocrinology2014，，4： | 2 |
| 4 | Applications and potential of ultrasonics in food processing显示文摘 | Dietrich K Marco Z Volker H | 2004 | Trends in Food Science & Technology2004，15，5： | 1 |
| 5 | Salinity-dependent switching of osmolyte strategies in a moderately Halophilic Bacterium: glutamate induces proline biosynthesis in Halobacillus halophilus显示文摘 | Stephan H S Volker M | 2007 | Journal of Bacteriology2007，189，19： | 1 |
| 6 | Thomashow components of the Arabidopsis C repeat/dehydration responsive element bindingfactor cold-response pathway are conserved in Brassica napus and other plant species显示文摘 | KIRSTEN R J SUSANNE K KEENAN L A XIN Z VOLKER H JAMES Z Z THOMAS D MICHAEL F | 2001 | Plant Plzysiology2001，127，3： | 1 |
| 7 | Applications and potential ofultrasonics in food processing显示文摘 | DietrichK Marco Z Volker H | 2004 | Trends in Food Science and Tech-nology2004，15，5： | 1 |
| 8 | La- ryngeal inflammatory myofibroblastic tumors: differ- ent clinical appearance and histomorphologic presenta- tion of one entity显示文摘 | VOLKER H U SCHEICH M ZETTL A | 2010 | Head Neck2010，32，： | 1 |
| 9 | Simultaneous manifestation of fulminant infectious mononucleosis with haemophagocytic syndrome and B-cell lymphoma in X-linked lymphoproliferative disease显示文摘 | Boris Hügle Itziar Astigarraga Jan-Inge Henter Anna Porwit-MacDonald Alfons Meindl Volker Schuster | 2007 | European Journal of Pediatrics2007，，6： | 1 |
| 10 | Carbon-nanofibre-reinforced poly(ether ether ketone) composites显示文摘 | Jan Sandler Philipp Werner Milo S.P Shaffer Vitaly Demchuk Volker Altst?dt Alan H Windle | 2002 | Composites Part A2002，，8： | 1 |
| 11 | Formation of thermal decomposition cavities in physical vapor transport of silicon carbide 显示文摘 | EDWARD K S THOMAS K VOLKER D H | 2000 | Journal of Electronic Materials2000，29，3： | 1 |
| 12 | The hepatitis B virus large surface prorein (LI-IBs) is a Tranacriptional activator 显示文摘 | Eberhard H Gesine S Volker B | 1996 | Virology1996，225，： | 1 |
| 13 | Regulation of erythropoiesis by hypoxia-induc- ible factors显示文摘 | Volker H | 2013 | Blood2013，27，1： | 1 |
| 14 | Granulocyte macrophage colony--stimulating factor deficiency affects vascular elastin production and integrity of elastic lamellae显示文摘 | Weissen--Plenz G Eschert H Volker W | 2008 | J Vasc Res2008，45，： | 1 |
| 15 | Industrial product - servicesystems (IPS2 ) 显示文摘 | Meier H Volker 0 Funke B | 2011 | The International Journal of AdvancedManufacturing Technology2011，52，11： | 1 |
| 16 | High-Performance Liquid-Chromatographic determination of free nicotinic acid and its metabolite,nicotinuric acid, in plasma and urine显示文摘 | Nikolaus H Volker S Michaela H | 1978 | Clinical Chemistry1978，24，： | 1 |
| 17 | Association of CYP1 B1 codon 432 mutant allele in head and neck squamous cell cancer is reflected by somatic mutations of P53 in tumor tissue显示文摘 | Yon K Josef A Volker H | 2001 | Cancer Res2001，61，： | 1 |
| 18 | Effects of pulsed electric fields on cell membrances in real food systems显示文摘 | Alexander A U Volker H Dietrich K | 2000 | Innovative Food Science and Emerging Technologies2000，1，2： | 1 |
| 19 | Event study concerning international bond price effects of credit rating actions 显示文摘 | MANFRED Steiner VOLKER G H | 2001 | International Journal of Finance & Economics2001，，6： | 1 |
| 20 | Tumor cellvaccinatin for gynecoloical tumor显示文摘 | Volker M Sigrif H Michael S T | 1993 | Hybridoma1993，12，5： | 1 |
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