|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Diarrhoea-predominant irritable bowel syndrome distinguishable by 16S rRNA gene phylotype quantification显示文摘AIM:To study whether selected bacterial 16S ribosomal RNA(rRNA)gene phylotypes are capable of disting- uishing irritable bowel syndrome(IBS). METHODS:The faecal microbiota of twenty volunteers with IBS,subdivided into eight diarrhoea-predominant (IBS-D),eight constipation-predominant(IBS-C)and four mixed symptom-subtype(IBS-M)IBS patients,and fifteen control subjects,were analysed at three time-points with a set of fourteen quantitative real-timepolymerase chain reaction assays.All assays targeted 16S rRNA gene phylotypes putatively associated with IBS,based on 16S rRNA gene library sequence analysis. The target phylotypes were affiliated with Actinobac-teria,Bacteroidetes and Firmicutes.Eight of the target phylotypes had less than 95%similarity to cultured bacterial species according to their 16S rRNA gene sequence.The data analyses were made with repeated-measures ANCOVA-type modelling of the data and principle component analysis(PCA)with linear mixed-effects models applied to the principal component scores. RESULTS:Bacterial phylotypes Clostridium cocleatum 88%,Clostridium thermosuccinogenes 85%,Coprobacillus catenaformis 91%,Ruminococcus bromii-like, Ruminococcus torques 91%,and R.torques 93%were detected from all samples analysed.A multivariate analysis of the relative quantities of all 14 bacterial 16S rRNA gene phylotypes suggested that the intestinal microbiota of the IBS-D patients differed from other sample groups.The PCA on the first principal component(PC1),explaining 30.36%of the observed variation in the IBS-D patient group,was significantly altered from all other sample groups(IBS-D vs control, P=0.01;IBS-D vs IBS-M,P=0.00;IBS-D vs IBS-C, P=0.05).Significant differences were also observed in the levels of distinct phylotypes using relative values in proportion to the total amount of bacteria.A phy- lotype with 85%similarity to C.thermosuccinogenes was quantified in significantly different quantities among the IBS-D and control subjects(-4.08±0.90 vs -3.33±1.16,P=0.04)and IBS-D and IBS-M subjects (-4.08±0.90 vs-3.08±1.38,P=0.05).Furthermore,a phylotype with 94%similarity to R.torques was more prevalent in IBS-D patients'intestinal micro- biota than in that of control subjects(-2.43±1.49 vs -4.02±1.63,P=0.01).A phylotype with 93%simi- larity to R.torques was associated with control sam- ples when compared with IBS-M(-2.41±0.53 vs -2.92±0.56,P=0.00).Additionally,a R.bromii-like phylotype was associated with IBS-C patients in com- parison to control subjects(-1.61±1.83 vs-3.69± 2.42,P=0.01).All of the above mentioned phylotype specific alterations were independent of the effect of time. CONCLUSION:Significant phylotype level alterationsin the intestinal microbiotas of IBS patients were observed,further emphasizing the possible contribution of the gastrointestinal microbiota in IBS. | Anna Lyra Teemu Rinttil Janne Nikkil Lotta Krogius-Kurikka Kajsa Kajander Erja Malinen Jaana Mtt Laura Mkel Airi Palva | 2009 | World Journal of Gastroenterology2009，15，47： | 26 |
| 2 | Association of symptoms with gastrointestinal microbiota in irritable bowel syndrome显示文摘AIM:To investigate the correlations between selfreported symptoms of irritable bowel syndrome(IBS) and the gastrointestinal(GI) microbiota composition.METHODS:Fecal samples were collected from a total of 44 subjects diagnosed with IBS.Their symptoms were monitored with a validated inflammatory bowel disease questionnaire adjusted for IBS patients.Thirteen quantitative real-time polymerase chain reaction assays were applied to evaluate the GI microbiota composition.Eubacteria and GI bacterial genera(Bifidobacterium,Lactobacillus and Veillonella),groups(Clostridium coccoides/Eubacterium rectale,Desulfovibrio desulfuricans) and distinct bacterial phylotypes [closest 16S rDNA sequence resemblance to species Bifidobacterium catenulatum,Clostridium cocleatum,Collinsella aerofaciens(C.aerofaciens),Coprococcus eutactus(C.eutactus),Ruminococcus torques and Streptococcus bovis ] with a suspected association with IBS were quantified.Correlations between quantities or presence/absence data of selected bacterial groups or phylotypes and various IBSrelated symptoms were investigated.RESULTS:Associations were observed between subjects' self-reported symptoms and the presence or quantities of certain GI bacteria.A Ruminococcus torques(R.torques)-like(94% similarity in 16S rRNA gene sequence) phylotype was associated with severity of bowel symptoms.Furthermore,among IBS subjects with R.torques 94% detected,the amounts of C.cocleatum 88%,C.aerofaciens-like and C.eutactus 97% phylotypes were significantly reduced.Interesting observations were also made concerning the effect of a subject's weight on GI microbiota with regard to C.aerofaciens like phylotype,Bifidobacterium spp.and Lactobacillus spp.CONCLUSION:Bacteria seemingly affecting the symptom scores are unlikely to be the underlying cause or cure of IBS,but they may serve as biomarkers of the condition. | Erja Malinen Lotta Krogius-Kurikka Janne Nikkil Airi Palva Anna Lyra Anne Jskelinen Teemu Rinttil Terttu Vilpponen-Salmela Atte Johannes von Wright | 2010 | World Journal of Gastroenterology2010，16，36： | 13 |
| 3 | Norway spruce(Picea abies) laccases:Characterization of a laccase in a lignin-forming tissue culture显示文摘Secondarily thickened cell walls of water-conducting vessels and tracheids and support-giving sclerenchyma cells contain lignin that makes the cell wal s water impermeable and strong. To what extent laccases and peroxidases contribute to lignin biosynthesis in muro is under active evaluation. We performed an in silico study of Norway spruce(Picea abies(L.)Karst.) laccases utilizing available genomic data. As many as 292 laccase encoding sequences(genes, gene fragments, and pseudogenes) were detected in the spruce genome. Out of the 112 genes annotated as laccases, 79 are expressed at some level. We isolated five full-length laccase c DNAs from developing xylem and an extracellular lignin-forming cell culture of spruce. In addition, we purified and biochemically characterized one culture medium laccase from the lignin-forming cell culture.This laccase has an acidic p H optimum(p H 3.8–4.2) for coniferyl alcohol oxidation. It has a high af finity to coniferyl alcohol with an apparent K m value of 3.5 m M; however, the laccase has a lowe catalytic ef ficiency(V max/K m) for coniferyl alcohol oxidation compared with some purified culture medium peroxidases. The properties are discussed in the context of the information already known about laccases/coniferyl alcohol oxidases o coniferous plants. | Sanna Koutaniemi Heli A.Malmberg Liisa K.Simola Teemu H.Teeri Anna K?rk?nen | 2015 | Journal of Integrative Plant Biology2015，57，4： | 4 |
| 4 | 花器发育调节基因gaga1转化大豆的初步研究显示文摘通过花粉管通道法用非洲菊基因 gaga1转化N2 899(质核互作雄性不育保持系 )、通州豆、95 - 7等大豆受体。处理的花朵总数为 4 30 0朵 ,共获 30 0 0多粒种子。经卡那霉素田间鉴定 ,获得了 14株抗卡那霉素的幼苗。通过抗性标记基因nptⅡ和目标基因 gaga1的PCR检测 ,发现有两株N2 899具有阳性信号 ,初步证明非洲菊基因 gaga1已整合到这两株大豆的基因组中。形态分析表明 :与未转化N2 899相比 ,转基因大豆株型矮小 ,始花期提前 ,盛花期花数量较少 ,结荚不多。显微分析发现 ,与对照相比转基因大豆花器官结构没有明显改变。 | 喻德跃 魏国兰 孙英 Teemu H Teeri | 2003 | 大豆科学2003，22，2： | 3 |
| 5 | A data-oriented (and beyond) network architecture显示文摘 | Teemu Koponen Mohit Chawla Byung-Gon Chun Andrey Ermolinskiy Kye Hyun Kim Scott Shenker Ion Stoica | 2007 | ACM SIGCOMM Computer Communication Review2007，，4： | 2 |
| 6 | 全膝关节置换翻修术的Meta分析显示文摘目的通过对已报道的全膝关节置换翻修术文献进行总结分析,讨探膝关节置换翻修术前后的膝关节功能、翻修的主要原因、主要并发症及不同假体的术后疗效。方法按照以下标准收集和分析有关全膝关节置换翻修术的文献:①1990年至2002年间发表,②报告患者数大于10例,③采用通用的膝关节评分标准。一名骨科专科医生独立收集数据,一名医学统计学专家独立采用Meta统计方法分析数据。结果共有33篇符合条件的文献被收集。患者共1356例,其中男429例,女611例(部分文献性别分类数据缺失),平均年龄67岁(45~90岁),加权平均随访时间57个月(6~108个月),加权平均术前膝关节功能总评分为49分(15~84分),术后为84分(58~109分),全膝关节置换翻修术前后的总评分、功能评分、活动范围等有显著性提高,差异有统计学意义(总评分:t=12.507,P<0.01,功能评分:t=4.704,P<0.01,活动范围:t=5.346,P<0.01)。全膝关节置换翻修术的原因主要是假体松动(55%),其它包括聚乙烯磨损(11%)、假体不稳(10%)、感染(7%)。翻修术后的主要并发症仍然为假体松动(18%),其它包括假体不稳(16%)、感染(16%)、髌骨问题(15%)及不明原因的膝关节疼痛(13%)。髌骨问题包括髌骨脱位、半脱位、髌韧带撕裂、髌股关节疼痛等。结论可以认为膝关节置换后翻修术是一种安全有效的手术。假体松动是膝关节置换翻修的主要原因和并发症。 | 盛璞义 Matti Lehto 廖威明 何爱珊 傅明 Matti Kataja 张紫机 Teemu Moilanen Jorma Pajamki Yrj T Konttinen | 2007 | 中华关节外科杂志(电子版)2007，1，1： | 2 |
| 7 | Unsteady Pressure Field in a Vaneless Diffuser of a Centrifugal Compressor: An Experimental and Computational Analysis显示文摘Unsteady static pressure was measured and time-accurate numerical simulations were conducted in order to analyze unsteady behaviour of the centrifugal compressor diffuser. The unsteady static pressure was measured at the diffuser inlet and outlet at three different circumferential positions. Operation points near the surge, the design operation point and operation point near the choke were used in measurements. The time-accurate simulations were conducted at the design operation point and operation point near the choke. The whole compressor was modelled and the low Reynolds number k-εturbulence model was utilized in numerical simulations. The gathered numerical and experimental unsteady pressures were compared with each other. | Teemu TURUNEN-SAARESTI Jaakko LARJOLA | 2004 | Journal of Thermal Science2004，13，4： | 2 |
| 8 | Arterial pulse wave velocity in relation to carotid intima-media thickness, brachialflow-mediated dilation and carotid artery distensibility: the Cardiovascular Risk in Young Finns Study and the Health 2000 Survey显示文摘 | Teemu Koivistoinen Marko Virtanen Nina Hutri-Khtnen | 2012 | Atherosclerosis2012，220，： | 1 |
| 9 | Data-based method for creating electricity use load profiles using large amount of customer-specific hourly measured electricity use data显示文摘 | Teemu R?s?nen Dimitrios Voukantsis Harri Niska Kostas Karatzas Mikko Kolehmainen | 2010 | Applied Energy2010，，11： | 1 |
| 10 | China,Russia and the Shanghai Cooperation Organisation:blessing or curse for new regionalism in Central Asia?显示文摘 | Teemu Naarajrvi | 2012 | Asia Europe Journal2012，10，23： | 1 |
| 11 | Moderate Impact of ERPs on Management Accounting a Lag or Permanent Outcome? 显示文摘 | MARKUS GRANLUND TEEMU MALMI | 2002 | Management Accounting Research2002，，13： | 1 |
| 12 | Reversible surface binding of cadmium and lead by lactic acid and bifidobacteria显示文摘 | Teemu H Seppo S Jussi M | 2008 | International journal of food microbiology2008，125，2： | 1 |
| 13 | Innovative Capacity, Educational Attainment and Economic Development in the European Union: Causal Relations and Geographical Variations显示文摘 | Teemu Makkonen Tommi Inkinen | 2013 | European Planning Studies2013，，12： | 1 |
| 14 | Arsenic Removal by Native and Chemically Modified Lacti- cacid Bateria 显示文摘 | TEEMU HALTTUNEN MATTIAS FINELL SEPPO SALMINEN | 2007 | International Journal of Food Microbiology2007，120，2007： | 1 |
| 15 | Moderate Impact of ERPS on Management Accounting:A Lag or Permanent Outcome 显示文摘 | Markus Garnlund Teemu Malmi | 2002 | Management Accounting Research2002，13，3： | 1 |
| 16 | Adenosine-induced cardiac arrest during intraoperative cerebral aneurysm rupture显示文摘 | Teemu Luostarinen Riikka S.K. Takala Tomi T. Niemi Ari J. Katila Mika Niemel? Juha Hernesniemi Tarja Randell | 2010 | World Neurosurgery2010，，2： | 1 |
| 17 | Polarised bidirectional reflectance factor measurements from soil, stones, and snow显示文摘 | Jouni Peltoniemi Teemu Hakala Juha Suomalainen Eetu Puttonen | 2009 | Journal of Quantitative Spectroscopy and Radiative Transfer2009，，17： | 1 |
| 18 | Value Based Management Practices Some Evidence from the Field 显示文摘 | Teemu Malmi Seppo Ikaheimo | 2003 | Management Accounting Research2003，14，3： | 1 |
| 19 | NOX : towards an operating system for networks 显示文摘 | Natasha Gude Teemu Koponen Justin Pettit | 2008 | ACM SIGCOMM Computer Commu- nication Review2008，38，3： | 1 |
| 20 | A Statistical Modeling Approach to Lo- cation Estimation 显示文摘 | Teemu Tonteri | 2002 | IEEE Transeationson Mobile Compu- ting2002，1，1： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员