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| 1 | A rapid advice guideline for the diagnosis and treatment of 2019 novel coronavirus(2019-nCoV) infected pneumonia(standard version)显示文摘In December 2019, a new type viral pneumonia cases occurred in Wuhan, Hubei Province;and then named '2019 novel coronavirus(2019-nCoV)' by the World Health Organization(WHO) on 12 January 2020. For it is a never been experienced respiratory disease before and with infection ability widely and quickly, it attracted the world’s attention but without treatment and control manual. For the request from frontline clinicians and public health professionals of 2019-nCoV infected pneumonia management, an evidence-based guideline urgently needs to be developed. Therefore, we drafted this guideline according to the rapid advice guidelines methodology and general rules of WHO guideline development;we also added the first-hand management data of Zhongnan Hospital of Wuhan University. This guideline includes the guideline methodology, epidemiological characteristics, disease screening and population prevention, diagnosis, treatment and control(including traditional Chinese Medicine), nosocomial infection prevention and control, and disease nursing of the 2019-nCoV. Moreover, we also provide a whole process of a successful treatment case of the severe 2019-nCoV infected pneumonia and experience and lessons of hospital rescue for 2019-nCoV infections. This rapid advice guideline is suitable for the first frontline doctors and nurses, managers of hospitals and healthcare sections, community residents, public health persons, relevant researchers, and all person who are interested in the 2019-nCoV. | Ying-Hui Jin Lin Cai Zhen-Shun Cheng Hong Cheng Tong Deng Yi-Pin Fan Cheng Fang Di Huang Lu-Qi Huang Qiao Huang Yong Han Bo Hu Fen Hu Bing-Hui Li Yi-Rong Li Ke Liang Li-Kai Lin Li-Sha Luo Jing Ma Lin-Lu Ma Zhi-Yong Peng Yun-Bao Pan Zhen-Yu Pan Xue-Qun Ren Hui-Min Sun Ying Wang Yun-Yun Wang Hong Weng Chao-Jie Wei Dong-Fang Wu Jian Xia Yong Xiong Hai-Bo Xu Xiao-Mei Yao Yu-Feng Yuan Tai-Sheng Ye Xiao-Chun Zhang Ying-Wen Zhang Yin-Gao Zhang Hua-Min Zhang Yan Zhao Ming-Juan Zhao Hao Zi Xian-Tao Zeng Yong-Yan Wang Xing-Huan Wang 无 | 2020 | Military Medical Research2020，7，1： | 161 |
| 2 | Mettl3-/Mettl14-mediated mRNA N6-methyladenosine modulates murine spermatogenesis显示文摘精子发生在双 spermatogonial 干细胞(SSC ) 哪个生产 haploid 精子期间是一个区别过程。这个高度专业化的过程精确在 transcriptional, posttranscriptional,和翻译层次被控制。这里,我们报导那 N 6-methyladenosine (m 6 一) ,调整基因表示的一个 epitranscriptomic 标记,在精子发生期间起必要作用。我们现在的全面 m 6 从五个发展阶段的老鼠 spermatogenic 房间的 mRNA methylomes:无差别的 spermatogonia,类型 A 1 spermatogonia, preleptotene spermatocytes, pachytene/diplotene spermatocytes,和圆 spermatids。m 6 有 Vasa-Cre 的 RNA methyltransferase Mettl3 或 Mettl14 引起 m 6 SSC 的 A 和弄空。m 6 为 SSC 增长 / 区别被要求的抄本的一个弄空 dysregulates 翻译。在有 Stra8-GFPCre 的先进细菌房间的 Mettl3 和 Mettl14 的联合删除破坏精子形式,而有在先进细菌房间的 Mettl3 或 Mettl14 的单个删除的老鼠显示出正常精子发生。从双异种老鼠展览的 spermatids 损害了为精子形式是必要的 haploid 特定的基因的翻译。这研究加亮 mRNA m 6 在 germline 开发的修正,潜在地保证在精子发生的不同阶段协调了翻译。 | Zhen Lin Phillip J Hsu Xudong Xing Jianhuo Fang Zhike Lu Qin Zou Ke-Jia Zhang Xiao Zhang Yuchuan Zhou Teng Zhang Youcheng Zhang Wanlu Song Guifang Jia Xuerui Yang Chuan He Ming-Han Tong | 2017 | Cell Research2017，27，10： | 61 |
| 3 | Rice DENSE AND ERECT PANICLE 2 is essential for determining panicle outgrowth and elongation显示文摘包括谷物尺寸和圆锥花序形态学,圆锥花序的建筑学直接决定谷物产量。圆锥花序直立,为在中国的北部分完成理想的植物建筑学被选择,引起了米饭 breeders 的增加的注意。这里,稠密、直立的圆锥花序 2 (dep2 ) 异种,显示出稠密、直立的圆锥花序显型,被识别。没有任何已知的功能的领域, DEP2 编码植物特定的蛋白质。表示介绍 DEP2 表明它高度在年轻纸巾被表示,与在年轻圆锥花序的大多数丰富。词法并且表示分析显示在 DEP2 的那个变化主要影响脊柱和主要、第二等的分支的快速的延伸,但是不损害圆锥花序 primordia 的开始或形成。进一步的分析建议在 dep2 的圆锥花序长度的减少被一个缺点在圆锥花序的指数的延伸期间在房间增长引起。尽管有在 dep2 异种的一种更紧缩的植物类型,在谷物生产的重要改变都没在野类型和 dep2 异种之间被发现。因此, DEP2 的学习不仅加强我们圆锥花序建筑学的分子的基因基础的理解而且为米饭繁殖有重要含意。 | Feng Li Wenbo Liu Jiuyou Tang Jinfeng Chen Hongning Tong Bin Hu Chunlai Li Jun Fang Mingsheng Chen Chengcai Chu | 2010 | Cell Research2010，20，7： | 51 |
| 4 | Relationship of nocturnal concentrations of melatonin, gamma-aminobutyric acid and total antioxidants in peripheral blood with insomnia after stroke: study protocol for a prospective non-randomized controlled trial显示文摘Melatonin and gamma-aminobutyric acid(GABA) have been shown to regulate sleep. The nocturnal concentrations of melatonin, GABA and total antioxidants may relate to insomnia in stroke patients. In this prospective single-center non-randomized controlled clinical trial performed in the China Rehabilitation Research Center, we analyzed the relationship of nocturnal concentrations of melatonin, GABA and total antioxidants with insomnia after stroke. Patients during rehabilitation of stroke were recruited and assigned to the insomnia group or non-insomnia group. Simultaneously, persons without stroke or insomnia served as normal controls. Each group contained 25 cases. The primary outcome was nocturnal concentrations of melatonin, GABA and total antioxidants in peripheral blood. The secondary outcomes were Pittsburgh Sleep Quality Index, Insomnia Severity Index, Epworth Sleepiness Scale, Fatigue Severity Scale, Morningness-Eveningness Questionnaire(Chinese version), and National Institute of Health Stroke Scale. The relationship of nocturnal concentrations of melatonin, GABA and total antioxidants with insomnia after stroke was analyzed and showed that they were lower in the insomnia group than in the non-insomnia group. The severity of stroke was higher in the insomnia group than in the non-insomnia group. Correlation analysis demonstrated that the nocturnal concentrations of melatonin and GABA were associated with insomnia after stroke. This trial was registered at Clinical Trials.gov, identifier: NCT03202121. | Wei Zhang Fang Li Tong Zhang | 2017 | Neural Regeneration Research2017，12，8： | 39 |
| 5 | Huangqi Guizhi Wuwu Decoction for treating diabetic peripheral neuropathy:a meta-analysis of 16 randomized controlled trials显示文摘OBJECTIVE:This meta-analysis was performed to systematically assess the efficacy and safety of the Chinese herbal medicine Huangqi Guizhi Wuwu Decoction(HGWWD) for treating diabetic peripheral neuropathy.DATA SOURCES:Six electronic databases,including the Cochrane Library,MEDLINE database,Chinese Biomedical Database,Chinese National Knowledge Infrastructure Database,Chinese Science and Technique Journals Database,and the Wanfang Database,were search ed on the internet for randomized controlled trials published up until 1 December 2015.The search terms included 'Chinese herbal medicine','diabetic peripheral neuropathy' and 'randomized controlled trials' in Chinese and in English.DATA SELECTION:We included randomized controlled trials using HGWWD/modified HGWWD for the treatment group,without restriction for the control group.We assessed literature quality in accordance with the Cochrane Review Handbook.A random or a fixed effects model was used to analyze outcomes using Rev Man 5.2 software.OUTCOME MEASURES:The primary outcomes were changes in symptoms and nerve conduction velocities.The secondary outcomeswere fasting blood glucose and hemorheological indexes.RESULTS:Sixteen randomized controlled trials,with a total of 1,173 patients,were included.Meta-analysis revealed that the efficacy of HGWWD for diabetic peripheral neuropathy was significantly superior compared with the control treatment(i.e.,control group)(risk ratio = 0.36,95% confidence interval(CI):0.29–0.46,Z =8.33,P < 0.00001) Compared with the control group,there was an increase in median motor nerve conduction velocity(mean difference(MD) = 3.46,95%CI:1.88–5.04,Z = 4.30,P < 0.01) and median sensory nerve conduction velocity(MD = 3.30,95%CI:2.04–4.56,Z = 5.14,P < 0.01).There was also an increase in peroneal motor nerve conduction velocity(MD = 3.22,95%CI:2.45–3.98,Z = 8.21,P < 0.01) and peroneal sensory nerve conduction velocity(MD = 3.05,95%CI:2.01–4.09,Z = 5.75,P < 0.01) in the treatment groups.No significant difference in fasting blood glucose was found between the treatment groups and the control groups(MD =-0.12,95%CI:-0.42–0.19,Z = 0.76,P = 0.45).Plasma viscosity was significantly decreased after treatment(MD =-0.11,95%CI:-0.21 to-0.02,Z = 2.30,P = 0.02).No significant difference in fibrinogen was detectable(MD =-0.53,95%CI:-1.28–0.22,Z = 1.38,P = 0.17).Four trials reported that treatment groups experienced no adverse reactions.Adverse events were not mentioned in the other 12 trials.No trial reported the incidence of complications,quality of life outcomes,or health economics.CONCLUSION:HGWWD treatment improves diabetic neurologic symptoms and ameliorates nerve conduction velocities.Our study suggests that HGWWD may have significant therapeutic efficacy for the treatment of diabetic peripheral neuropathy.However,the methodological quality of the randomized controlled trials was generally low.Larger and better-designed randomized controlled trials are required to more reliably assess the clinical effectiveness of HGWWD. | Bing Pang Tian-yu Zhao Lin-hua Zhao Fang Wan Ru Ye Qiang Zhou Feng Tian Xiao-lin Tong | 2016 | Neural Regeneration Research2016，11，8： | 32 |
| 6 | Role of epithelial-mesenchymal transition in gastric cancer initiation and progression显示文摘Gastric cancer is one of the most common malignant tumors worldwide.Due to its intricate initiation and progression mechanisms,early detection and effective treatment of gastric cancer are difficult to achieve.The epithelial-mesenchymal transition(EMT)is characterized as a fundamental process that is critical for embryonic development,wound healing and fibrotic disease.Recent evidence has established that aberrant EMT activation in the human stomach is closely associated with gastric carcinogenesis and tumor progression.EMT activation endows gastric epithelial cells with increased characteristics of mesenchymal cells and reduces their epithelial features.Moreover,mesenchymal cells tend to dedifferentiate and acquire stem cell or tumorigenic phenotypes such as invasion,metastasis and apoptosis resistance as well as drug resistance during EMT progression.There are a number of molecules that indicate the stage of EMT(e.g.,E-cadherin,an epithelial cell biomarker);therefore,certain transcriptional proteins,especially E-cadherin transcriptional repressors,may participate in the regulation of EMT.In addition,EMT regulation may be associated with certain epigenetic mechanisms.The aforementioned molecules can be used as early diagnostic markers for gastric cancer,and EMT regulation can provide potential targets for gastric cancer therapy.Here,we review the role of these aspects of EMT in gastric cancer initiation and development. | Zhao Peng Chen-Xiao Wang Er-Hu Fang Guo-Bin Wang Qiang Tong | 2014 | World Journal of Gastroenterology2014，20，18： | 28 |
| 7 | PTEN-L is a novel protein phosphatase for ubiquitin dephosphorylation to inhibit PINK1-Parkin-mediated mitophagy显示文摘Mitophagy 是为损坏线粒体的特定的消除的选择 autophagy 的一种重要类型。(PINK1 )导致 PTEN 的通常认为的 kinase 蛋白质 1 催化 ubiquitin (Ub ) 的 phosphorylation 在 PINK1-Parkin-mediated mitophagy 的发作起一个关键作用。(PTEN-L ) 磷酸酶和 tensin 相当或相同的事物(PTEN ) 长是 PTEN 的最新识别的 isoform,与到它的 N 终点的 173 氨基酸的增加。这里,我们报导 PTEN-L 是经由它对 phosphorylated ubiquitin 的蛋白质磷酸酶活动的 mitophagy 的一个新奇否定管理者。我们发现 PTEN-L 在外部 mitochondrial 膜(OMM ) 本地化,而 PTEN-L 的删除支持, PTEN-L 的 overexpression 禁止, mitophagy 由各种各样的损坏线粒体的代理人导致了。机械学地, PTEN-L 能够有效地阻止 Parkin mitochondrial translocation,减少 Parkin phosphorylation,维持它的关上的不活跃的符合构造,并且禁止它的 E3 ligase 活动。更重要地, PTEN-L 在 vivo 减少 phosphorylated ubiquitin (pSer65-Ub ) 的水平,并且在 vitro,磷酸酶试金经由它的蛋白质磷酸酶活动证实那 PTEN-L dephosphorylates pSer65-Ub,独立于它的类脂化合物磷酸酶功能。一起拿,我们的调查结果为 ubiquitin 作为蛋白质磷酸酶表明 PTEN-L 的新奇功能,它抵抗在 mitophagy 正式就职和 mitophagy 的最终的抑制导致前馈控制机制的阻塞的调停 PINK1 的 ubiquitin phosphorylation。因此,理解 PTEN-L 的这新奇功能在控制 mitophagy 的分子的难题提供一关键错过片,在包括象 Parkinsons 那样的 neurodegenerative 混乱的许多重要人的疾病的一个批评过程疾病。 | Liming Wang Yik-Lam Cho Yancheng Tang Jigang Wang Jung-Eun Park Yajun Wu Chunxin Wang Yan Tong Ritu Chawla Jianbin Zhang Yin Shi Shuo Deng Guang Lu Yihua Wu Hayden Weng-Siong Tan Pornteera Pawijit Grace Gui-Yin Lim Hui-Ying Chan Jingzi Zhang Lei Fang Hanry Yu Yih-Cherng Liou Mallilankaraman Karthik Boon-Huat Bay Kah-Leong Lim Siu-Kwan Sze Celestial T. Yap Han-Ming Shen | 2018 | Cell Research2018，28，8： | 25 |
| 8 | Locking internal fixator with minimally invasive plate osteosynthesis for the proximal and distal tibial frac- tures显示文摘ObjectiveTo 调查锁住的功效内部 fixator ( LIF )包括锁住的压缩板(人员登陆艇)和不太侵略的稳定的系统( LISS ),在 98 的一个总数上在近似、远侧的胫骨的 fractures.MethodsWe 做了回顾的研究由最低限度地侵略的板 osteosynthe-sis ( MIPO )与 LIF 收到了手术的有到2007年1月的从2003年1月的近似或远侧的胫骨的破裂的病人技术。数据由 43 近似胫骨的破裂(类型 AO41C3 ) 和 55 远侧的胫骨的破裂(类型 AO43C3 ) 组成了 .ResultsNo 复杂并发症在操作以后在所有病人被观察。吝啬的愈合时间是 8.4 个月(范围 5-14 月) 。仅仅推迟的联合的二个案例发生了在手术后 10 个月。没有感染甚至在开的破裂的情况中在权威的外科以后被报导。所有病人到达了全套运动在手术后 6 ~ 9 个月并且在 MIPO 重获膝和脚关节 joints.ConclusionUsing LIF 的正常功能技术是向不对钉的 intramedullary 合适的近似、远侧的胫骨的破裂的一条可靠途径。 | TONG Da-ke JI Fang CAI Xiao-bing | 2011 | Chinese Journal of Traumatology2011，14，4： | 20 |
| 9 | Comparison of four prognostic models and a new Logistic regression model to predict short-term prognosis of acute-on-chronic hepatitis B liver failure显示文摘 | HE Wei-ping HU Jin-hua ZHAO Jun TONG Jing-jing DING Jin-biao LIN Fang WANG Hui-fen | 2012 | Chinese Medical Journal2012，，13： | 19 |
| 10 | Applications of electrospun nanofibers显示文摘Polymeric nanofiber non-woven materials produced by electrospinning have extremely high sur-face-to-mass (or volume) ratio and a porous structure with excellent pore-interconnectivity. These characteristics plus the functionalities and surface chemistry of the polymer itself impart the nanofi-bers with desirable properties for a range of advanced applications. This review summarizes the recent progress in electrospun nanofibers, with an emphasis on their applications. | FANG Jian NIU HaiTao LIN Tong WANG XunGai | 2008 | Chinese Science Bulletin2008，53，15： | 19 |
| 11 | Single whispering-gallery mode lasing in polymer bottle microresonators via spatial pump engineering显示文摘Single-mode lasing in whispering-gallery mode(WGM)microresonators is challenging to achieve.In bottle microresonators,the highly non-degenerated WGMs are spatially well-separated along the long-axis direction and provide mode-selection capability.In this work,by engineering the pump intensity to modify the spatial gain profiles of bottle microresonators,we demonstrate a simple and general approach to realizing single-mode WGM lasing in polymer bottle microresonators.The pump intensity is engineered into an interference distribution on the bottle microresonator surface.By tuning the spacing between axial positions of the interference pump patterns,the mode intensity profiles of single-bottle WGMs can be spatially overlapped with the interference stripes,intrinsically enabling single-mode lasing and selection.Attractive advantages of the system,including high sidemode suppression factors 420 dB,large spectral tunability 48 nm,low-lasing threshold and reversible control,are presented.Our demonstrated approach may have a variety of promising applications,ranging from tunable single-mode lasing and sensing to nonlinear optics. | Fuxing Gu Fuming Xie Xing Lin Shuangyi Linghu Wei Fang Heping Zeng Limin Tong Songlin Zhuang | 2017 | Light(Science & Applications)2017，6，1： | 17 |
| 12 | Motor imagery training induces changes in brain neural networks in stroke patients显示文摘Motor imagery is the mental representation of an action without overt movement or muscle activation. However, the effects of motor imagery on stroke-induced hand dysfunction and brain neural networks are still unknown. We conducted a randomized controlled trial in the China Rehabilitation Research Center. Twenty stroke patients, including 13 males and 7 females, 32–51 years old, were recruited and randomly assigned to the traditional rehabilitation treatment group(PP group, n = 10) or the motor imagery training combined with traditional rehabilitation treatment group(MP group, n = 10). All patients received rehabilitation training once a day, 45 minutes per session, five times per week, for 4 consecutive weeks. In the MP group, motor imagery training was performed for 45 minutes after traditional rehabilitation training, daily. Action Research Arm Test and the Fugl-Meyer Assessment of the upper extremity were used to evaluate hand functions before and after treatment. Transcranial magnetic stimulation was used to analyze motor evoked potentials in the affected extremity. Diffusion tensor imaging was used to assess changes in brain neural networks. Compared with the PP group, the MP group showed better recovery of hand function, higher amplitude of the motor evoked potential in the abductor pollicis brevis, greater fractional anisotropy of the right dorsal pathway, and an increase in the fractional anisotropy of the bilateral dorsal pathway. Our findings indicate that 4 weeks of motor imagery training combined with traditional rehabilitation treatment improves hand function in stroke patients by enhancing the dorsal pathway. This trial has been registered with the Chinese Clinical Trial Registry(registration number: Chi CTR-OCH-12002238). | Fang Li Tong Zhang Bing-Jie Li Wei Zhang Jun Zhao Lu-Ping Song | 2018 | Neural Regeneration Research2018，13，10： | 15 |
| 13 | Biosorption of Cu(Ⅱ) on extracellular polymers from Bacillus sp.F19显示文摘Biosorption can be an effective process for the removal of heavy metals from aqueous solutions.The adsorption of Cu(Ⅱ) from aqueous solution on the extracellular polymers (EPS) from Bacillus sp.(named MBFF19) with respect to pH,incubation time,concentration of initial Cu(Ⅱ),and biosorbent dose was studied.Biosorption of Cu(Ⅱ) is highly pH dependent.The maximum uptake of Cu(Ⅱ) (89.62 mg/g) was obtained at pH 4.8.Biosorption equilibrium was established in approximately 10 min.The correlation coeffcient of more than 0.90 turned out that the adsorption process of Cu(Ⅱ) on MBFF19 was in accordance with both Langmuir and Freundlich isotherms.The pseudo-first and second order models were applied to examine the kinetics of the adsorption,whereas the latter was found to be in harmony with the kinetic data better.Because of the outstanding uptake capacity of Cu(Ⅱ),MBFF19 produced by Bacillus sp.was proved to be an excellent biosorbent for removing Cu(Ⅱ) from aqueous solutions. | ZHENG Yan,FANG Xuliang,YE Zhilong,LI Yahong,CAI weimin School of Environmental Science and Engineering,Shanghai Jiao Tong University,Shanghai 200240,China. | 2008 | Journal of Environmental Sciences2008，20，11： | 15 |
| 14 | Visible light-induced degradation of organic pollutants using Fe(II) supported on silica gel as an effective catalyst显示文摘Silica gel-supported Fe(II) (SiOFe) was prepared and used for heterogeneous degradation of sulforho-damine B (SRB) and 2,4-dichlorophenol (DCP) under visible irradiation (λ > 420 nm) as an effective catalyst. UVvisible spectra, and infrared Spectrophotometry (IR), fluorescence, total organic carbon (TOC) and electron spin resonance (ESR) measurements were employed to analyze the photoreaction products. The results showed that SRB could be efficiently degraded by SiOFe/H2O2 system under visible irradiation with 100% decolorization and 72.3% TOC removal after 180 min illumination. The results of ESR and fluorescence measurements indicated that the oxidative process was predominated mainly by the hydroxyl radical (·OH) generated in the system. | ZHAO Chao HUANG YingPing FANG YanFen JIANG LiRong LIU LiMing King Tong Lau | 2008 | Chinese Science Bulletin2008，53，10： | 13 |
| 15 | Ultrasensitive skin‐like wearable optical sensors based on glass micro/nanofibers显示文摘Electronic skin,a class of wearable electronic sensors that mimic the functionalities of human skin,has made remarkable success in applications including health monitoring,human-machine interaction and electronic-biological interfaces.While electronic skin continues to achieve higher sensitivity and faster response,its ultimate performance is fundamentally limited by the nature of low-frequency AC currents.Herein,highly sensitive skin-like wearable optical sensors are demonstrated by embedding glass micro/nanofibers(MNFs)in thin layers of polydimethylsiloxane(PDMS).Enabled by the transition from guided modes into radiation modes of the waveguiding MNFs upon external stimuli,the skin-like optical sensors show ultrahigh sensitivity(1870 k·Pa^-1),low detection limit(7 mPa)and fast response(10μs)for pressure sensing,significantly exceeding the performance metrics of state-of-the-art electronic skins.Electromagnetic interference(EMI)-free detection of high-frequency vibrations,wrist pulse and human voice are realized.Moreover,a five-sensor optical data glove and a 2×2-MNF tactile sensor are demonstrated.These initial results pave the way toward a new category of optical devices ranging from ultrasensitive wearable sensors to optical skins. | Lei Zhang Jing Pan Zhang Zhang Hao Wu Ni Yao Dawei Cai Yingxin Xu Jin Zhang Guofei Sun Liqiang Wang Weidong Geng Wenguang Jin Wei Fang Dawei Di Limin Tong | 2020 | Opto-Electronic Advances2020，3，3： | 12 |
| 16 | Seven-day triple therapy is a better choice for Helicobacter pylori eradication in regions with low antibiotic resistance显示文摘AIM: To investigate whether 7-d triple therapies are still valid in populations with low levels of resistance.METHODS: A total of 1106 Helicobacter pylori(H. pylori)-positive patients were divided into three groups,each of which received one type of 7-d triple therapy. Therapeutic outcomes of the patients were assessed by the 13C-urea breath test at 8 wk after treatment. The susceptibility of H. pylori to antibiotics was determined by an agar-dilution method. Data analysis was performed by χ2 tests.RESULTS: The eradication rates in groups A,B and C were 90.71%(332/366),90.46%(313/346) and 90.87%(189/208),respectively(P = 0.986). The resistance rates were 8.91% for clarithromycin,14.78% for levofloxacin and 0% for amoxicillin. The eradication rate was significantly different between clarithromycin-and levofloxacin-resistant patients(P < 0.05) in group A. Patients whose treatment failed in group A also had a higher clarithromycin resistance rate than did successive patients(P = 0.034). However,levofloxacin resistance had no obvious influence on the eradication rate. Furthermore,three main antibiotics(clarithromycin,levofloxacin and amoxicillin) had lower DID(defined daily dose per 1000 inhabitants per day) in this city.CONCLUSION: Clarithromycin resistance is the main reason for the failure of 7-d triple therapy. In populations with low levels of resistance,a 7-d triple therapy is a viable choice. The choice of therapy should not be influenced by conditions in high antibiotic resistance regions. | Yue-Feng Tong Jun Lv Li-Yuan Ying Fang Xu Bo Qin Ming-Tong Chen Fei Meng Miao-Ying Tu Ning-Min Yang You-Ming Li Jian-Zhong Zhang | 2015 | World Journal of Gastroenterology2015，21，46： | 12 |
| 17 | Hepatitis C virus in human B lymphocytes transformed by Epstein-Barr virus in vitro by in situ reverse transcriptase-polymerase chain reaction显示文摘AIM To study persistence and replication ofheltitis C virus (HCV) in patients' peripheralblood mononuclear cells (PBMC) cultured invitro.METHODS Epstein-Barr virus (EBV) was usedto transform the hepatitis C virus from a HCVpositive patient to permanent lymphoblastoidcell lines (LCL). Positive and negative HCV RNAstrands of the cultured cells and growth mediawere detected by reverse transcriptase-polymerase chain reaction ( RT-PCR ) eachmonth. Core and NS5 proteins of HCV werefurther tested using immunohistochemical SPmethod and in situ RT-PCR.RESULTS HCV RNA positive strands wereconsistently detected the cultured cells for oneyear. The negative-strand RNA in LCL cells andthe positive-strand RNA in supernatants wereobserved intermittently. Immunohistochemicalresults medicated expression of HCV NS3 and Cproteins in LCL cytoplasm mostly. The positivesignal of PCR product was dark blue and mainlylocalized to the LCL cytoplasm. The RT-PCRsignal was eliminated by overnight RNasedigestion but not DNase digestion.CONCLUSION HCV may exist and remainfunctional in a cultured cell line for a longperiod. | Ji Lin Cheng Bao Ling Liu Yi Zhang Wen Bin Tong Zheng Yan Bai Fang Feng Institute of Hepatology,Peoples Hospital,Medical Center of Beijing University,Beijing 10(X)44,China | 2001 | World Journal of Gastroenterology2001，7，3： | 11 |
| 18 | Multiple Risk Factor Clustering and Risk of Hypertension in the Mongolian Ethnic Population of China显示文摘Objective To evaluate whether the clustering of risk factors, both environmental and genetic, increases the risk of essential hypertension (EH) and the accumulation of risk factors influences the blood pressure level in normotensives. Methods On the basis of a prevalence survey, 501 subjects of Mongolian ethnicity (243 hypertensives and 258 normotensives) who were not related to each other were selected to conduct a case-control study. All subjects were interviewed with questionnaires and their blood specimens were collected. Renin gene insertion/deletion (I/D) polymorphism, a new genetic marker, was genotyped with PCR and polyacrylamide gel electrophoresis. Results Overweight, alcohol consumption, and renin gene I/D polymorphism were significant risk factors of EH (P<0.05). The odds ratios (OR) for the number of risk factors were 2.39 (95%CI: 0.98-6.74) for one risk factor, 5.03 (95%CI: 2.06-14.18) for two, and 6.09 (95%CI: 1.85-22.38) for three respectively after adjusting for age and sex. In normotensives, age- and sex-adjusted mean blood pressures increased with more accumulation of risk factors. However, there were no significant differences among the different blood pressure levels according to the number of risk factors (P>0.05). Conclusion Overweight, alcohol consumption, and renin gene I/D polymorphism are risk factors of EH in the Mongolian ethnic population of China. The accumulation of the risk factors causes a sharp increase of the risk of EH. | CHANG-QING YING SONG-BIN FU QUN XU WEI-JUN TONG MING-WU FANG ZHENG-LAI WU CHANG-CHUN OIU YONG-HONG ZHANG | 2007 | Biomedical and Environmental Sciences2007，20，5： | 11 |
| 19 | Antibiotic Resistance Genes in the Human-Impacted Environment:A One Health Perspective显示文摘Antibiotic resistance and its environmental component are gaining more attention as part of combating the growing healthcare crisis. The One Health framework, promulgated by many global health agencies, recognizes that antimicrobial resistance is a truly inter-domain problem in which human health, animal agriculture, and the environment are the core and interrelated components.This prospectus presents the status and issues relevant to the environmental component of antibiotic resistance, namely, the needs for advancing surveillance methodology: the environmental reservoirs and sources of resistance, namely, urban wastewater treatment plants, aquaculture production systems, soil receiving manure and biosolid, and the atmosphere which includes longer range dispersal.Recently, much work has been done describing antibiotic resistance genes in various environments;now quantitative, mechanistic,and hypothesis-driven studies are needed to identify practices that reduce real risks and maintain the effectiveness of our current antibiotics as long as possible. Advanced deployable detection methods for antibiotic resistance in diverse environmental samples are needed in order to provide the surveillance information to identify risks and define barriers that can reduce risks. Also needed are practices that reduce antibiotic use and thereby reduce selection for resistance, as well as practices that limit the dispersal of or destroy antibiotic-resistant bacteria or their resistance genes that are feasible for these varied environmental domains. | James M. TIEDJE WANG Fang Celia M. MANAIA Marko VIRTA SHENG Hongjie MA Liping ZHANG Tong Edward TOPP | 2019 | Pedosphere2019，29，3： | 11 |
| 20 | The positive function of selenium supplementation on reducing nitrate accumulation in hydroponic lettuce(Lactuca sativa L.)显示文摘High nitrate(NO_3^- ) in vegetables, especially in leaf vegetables poses threaten to human health. Selenium(Se) is an important element for maintaining human health, and exogenous Se application during vegetable and crop production is an effective way to prevent Se deficiency in human bodies. Exogenous Se shows positive function on plant growth and nutrition uptake under abiotic and/or biotic stresses. However, the influence of exogenous Se on NO_3^- accumulation in hydroponic vegetables is still not clear. In the present study, hydroponic lettuce plants were subjected to six different concentrations(0, 0.1, 0.5, 5, 10 and 50 μmol L–1) of Se as Na2 Se O3. The effects of Se on NO_3^- content, plant growth, and photosynthetic capacity of lettuce(Lactuca sativa L.) were investigated. The results showed that exogenous Se positively decreased NO_3^- content and this effect was concentration-dependent. The lowest NO_3^- content was obtained under 0.5 μmol L–1 Se treatment. The application of Se enhanced photosynthetic capacity by increasing the photosynthesis rate(Pn), stomatal conductance(Cs) and the transpiration efficiency(Tr) of lettuce. The transportation and assimilation of NO_3^- and activities of nitrogen metabolism enzymes in lettuce were also analysed. The NO_3^- efflux in the lettuce roots was markedly increased, but the efflux of NO_3^- from the root to the shoot was decreased after treated with exogenous Se. Moreover, Se application stimulated NO_3^- assimilation by enhancing nitrate reductase(NR), nitrite reductase(Ni R), glutamine synthetase(GS) and glutamate synthase enzyme(GOGAT) activities. These results provide direct evidence that exogenous Se shows positive function on decreasing NO_3^- accumulation via regulating the transport and enhancing activities of nitrogen metabolism enzyme in lettuce. We suggested that 0.5 μmol L–1 Se can be used to reduce NO_3^- content and increase hydroponic lettuce yield. | LEI Bo BIAN Zhong-hua YANG Qi-chang WANG Jun CHENG Rui-feng LI Kun LIU Wen-ke ZHANG Yi FANG Hui TONG Yun-xin | 2018 | Journal of Integrative Agriculture2018，17，4： | 9 |
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