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| 1 | PBS3 and EPS1 Complete Salicylic Acid Biosynthesis from Isochorismate in Arabidopsis显示文摘Salicylic acid(SA)is an important phytohormone mediating both local and systemic defense responses in plants.Despite over half a century of research,how plants biosynthesize SA remains unresolved.In Arabidop-sis,a major part of SA is derived from isochorismate,a key intermediate produced by the isochorismate syn-thase,which is reminiscent of SA biosynthesis in bacteria.Whereas bacteria employ an isochorismate pyru-vate lyase(IPL)that catalyzes the turnover of isochorismate to pyruvate and SA,plants do not contain an IPL ortholog and generate SA from isochorismate through an unknown mechanism.Combining genetic and biochemical approaches,we delineated the SA biosynthetic pathway downstream of isochorismate in Ara-bidopsis.We found that PBS3,a GH3 acyl adenylase-family enzyme important for SA accumulation,catalyzes ATP-and Mg2+-dependent conjugation of L-glutamate primarily to the 8-carboxyl of isochorismate and yields the key SA biosynthetic intermediate,isochorismoyl-glutamate A.Moreover,we discovered that EPS1,a BAHD acyltransferase-family protein with a previously implicated role in SA accumulation upon pathogen attack,harbors a noncanonical active site and an unprecedented isochorismoyl-glutamate A pyruvoyl-glutamate lyase activity that produces SA from the isochorismoyl-glutamate A substrate.Together,PBS3 and EPS1 form a two-step metabolic pathway to produce SA from isochorismate in Arabidopsis,which is distinct from how SA is biosynthesized in bacteria.This study closes a major knowledge gap in plant SA meta-bolism and would help develop new strategies for engineering disease resistance in crop plants. | Michael P.Torrens-Spence Anastassia Bobokalonova Valentina Carballo Christopher MGlinkerman Toma(s)Pluskal Amber Shen Jing-Ke Weng | 2019 | Molecular Plant2019，12，12： | 10 |
| 2 | Titanium dioxide induced inflammation in the small intestine显示文摘AIM:To investigate the effects of titanium dioxide (TiO2) nanoparticles (NPTiO 2 ) and microparticles (MPTiO 2 ) on the inflammatory response in the small intestine of mice. METHODS: Bl 57/6 male mice received distilled water suspensions containing TiO 2 (100 mg/kg body weight) as NPTiO 2 (66 nm), or MPTiO 2 (260 nm) by gavage for 10 d, once a day; the control group received only distilled water. At the end of the treatment the duodenum, jejunum and ileum were extracted for assessment of cytokines, inflammatory cells and titanium content. The cytokines interleukin (IL)-1b, IL-4, IL-6, IL-8, IL-10, IL-12, IL-13, IL-17, IL-23, tumor necrosis factor-α (TNF-α), intracellular interferon-γ (IFN-γ) and transforming growth factor-β (TGF-β) were evaluated by enzyme-linked immunosorbent assay in segments of jejunum and ileum (mucosa and underlying muscular tissue). CD4 + and CD8 + T cells, natural killer cells, and dendritic cells were evaluated in duodenum, jejunum and ileum samples fixed in 10% formalin by immuno-histochemistry. The titanium content was determined by inductively coupled plasma atomic emission spectrometry. RESULTS: We found increased levels of T CD4 + cells (cells/mm 2 ) in duodenum:NP 1240 ± 139.4, MP 1070 ± 154.7 vs 458 ± 50.39 (P < 0.01); jejunum:NP 908.4 ± 130.3, MP 813.8 ± 103.8 vs 526.6 ± 61.43 (P < 0.05); and ileum:NP 818.60 ± 123.0, MP 640.1 ± 32.75 vs 466.9 ± 22.4 (P < 0.05). In comparison to the control group, the groups receiving TiO 2 showed a statistically significant increase in the levels of the inflammatory cytokines IL-12, IL-4, IL-23, TNF-α, IFN-γ and TGF-β. The cytokine production was more pronounced in the ileum (mean ± SE):IL-12: NP 33.98 ± 11.76, MP 74.11 ± 25.65 vs 19.06 ± 3.92 (P < 0.05); IL-4: NP 17.36 ± 9.96, MP 22.94 ± 7.47 vs 2.19 ± 0.65 (P < 0.05); IL-23: NP 157.20 ± 75.80, MP 134.50 ± 38.31 vs 22.34 ± 5.81 (P < 0.05); TNFα: NP 3.71 ± 1.33, MP 5.44 ± 1.67 vs 0.99± 019 (P < 0.05); IFNγ: NP 15.85 ± 9.99, MP 34.08 ± 11.44 vs 2.81 ± 0.69 (P < 0.05); and TGF-β: NP 780.70 ± 318.50, MP 1409.00 ± 502.20 vs 205.50 ± 63.93 (P < 0.05). CONCLUSION:Our findings indicate that TiO2 particles induce a Th1-mediated inflammatory response in the small bowel in mice. | Carolina Maciel Nogueira Walter Mendes de Azevedo Maria Lucia Zaidan Dagli Sérgio Hiroshi Toma AndréZonetti de Arruda Leite Maria Laura Lordello Iêda Nishitokukado Carmen Lúcia Ortiz-Agostinho Maria IrmaSeixas Duarte Marcelo Alves Ferreira Aytan Miranda Sipahi | 2012 | World Journal of Gastroenterology2012，18，34： | 8 |
| 3 | Second-line Therapy With Levofloxacin After Failure of Treatment to Eradicate Helicobacter pylori Infection: Time Trends in a Spanish Multicenter Study of 1000 Patients显示文摘 | Javier P. Gisbert ángeles Pérez-Aisa Fernando Bermejo Manuel Castro-Fernández Pedro Almela Jesús Barrio ángel Cosme Inés Modolell Felipe Bory Miguel Fernández-Bermejo Luis Rodrigo Jesús Ortu?o Pilar Sánchez-Pobre Sam Khorrami Alejandro Franco Albert Tomas | 2013 | Journal of Clinical Gastroenterology2013，，2： | 5 |
| 4 | Multiple metastases to the small bowel from large cell bronchial carcinomas显示文摘AIM: Metastases from lung cancer to gastrointestinal tract are not rare atpostmortem studies but the development of clinically significant symptoms from the gastrointestinal metastases is very unusual.METHODS: Formalin-fixed, paraffin-embedded tissues were cut into 5 μm thick sections and routinely stained with hematoxylin and eosin. Some slides were also stained with Alcian-PAS. Antibodies used were primary antibodies to pancytokeratin, cytokeratin 7, cytokeratin 20, epithelial membrane antigen, vimentin, smooth muscle actin and CD-117.RESULTS: We observed three patients who presented with multiple metastases from large cell bronchial carcinoma to small intestine. Two of them had abdominal symptoms (sudden onset of abdominal pain, constipation and vomiting) and in one case the tumor was incidentally found during autopsy. Microscopically, all tumors showed a same histological pattern and consisted almost exclusively of strands and sheets of poorly cohesive, polymorphic giant cells with scanty, delicate stromas. Few smaller polygonal anaplastic cells dispersed between polymorphic giant cells,were also observed. Immunohistochemistry showed positive staining of the tumor cells with cytokeratin and vimentin. Microscopically and immunohistochemically all metastases had a similar pattern to primary anaplastic carcinoma of the small intestine.CONCLUSION: In patients with small intestine tumors showing anaplastic features, especially with multiple tumors, metastases from large cell bronchial carcinoma should be first excluded, because it seems that they are more common than expected. | Davor Tomas Mario Ledinsky Mladen Belicza Bozo Kru(s|ˇ)lin | 2005 | World Journal of Gastroenterology2005，11，9： | 4 |
| 5 | A techno-economic and life cycle assessment for the production of green methanol from CO_(2): catalyst and process bottlenecks显示文摘The success of catalytic schemes for the large-scale valorization of CO_(2) does not only depend on the development of active,selective and stable catalytic materials but also on the overall process design.Here we present a multidisciplinary study(from catalyst to plant and techno-economic/lifecycle analysis)for the production of green methanol from renewable H2 and CO_(2).We combine an in-depth kinetic analysis of one of the most promising recently reported methanol-synthesis catalysts(InCo)with a thorough process simulation and techno-economic assessment.We then perform a life cycle assessment of the simulated process to gauge the real environmental impact of green methanol production from CO_(2).Our results indicate that up to 1.75 ton of CO_(2) can be abated per ton of produced methanol only if renewable energy is used to run the process,while the sensitivity analysis suggest that either rock-bottom H2 prices(1.5$kg1)or severe CO_(2) taxation(300$per ton)are needed for a profitable methanol plant.Besides,we herein highlight and analyze some critical bottlenecks of the process.Especial attention has been paid to the contribution of H2 to the overall plant costs,CH4 trace formation,and purity and costs of raw gases.In addition to providing important information for policy makers and industrialists,directions for catalyst(and therefore process)improvements are outlined. | Tomas Cordero-Lanzac Adrian Ramirez Alberto Navajas Lieven Gevers Sirio Brunialti Luis MGandía Andrés T.Aguayo S.Mani Sarathy Jorge Gascon | 2022 | Journal of Energy Chemistry2022，31，5： | 3 |
| 6 | A Variational Finite Element Model for Large-Eddy Simulations of Turbulent Flows显示文摘The authors introduce a new Large Eddy Simulation model in a channel,based on the projection on finite element spaces as filtering operation in its variational form,for a given triangulation{Th}h>0.The eddy viscosity is expressed in terms of the friction velocity in the boundary layer due to the wall,and is of a standard sub grid-model form outside the boundary layer.The mixing length scale is locally equal to the grid size.The computational domain is the channel without the linear sub-layer of the boundary layer.The no-slip boundary condition(or BC for short)is replaced by a Navier(BC)at the computational wall.Considering the steady state case,the authors show that the variational finite element model they have introduced,has a solution(vh,ph)h>0that converges to a solution of the steady state Navier-Stokes equation with Navier BC. | Toma's Chac'on REBOLLO Roger LEWANDOWSKI | 2013 | Chinese Annals of Mathematics,Series B2013，34，5： | 2 |
| 7 | Epigenetic modification of liver mitochondrial DNA is associated with histological severity of nonalcoholic fatty liver disease显示文摘 | Carlos Jose Pirola Tomas Fernández Gianotti Adriana Laura Burgue?o Manuel Rey-Funes Cesar Fabian Loidl Pablo Mallardi Julio San Martino Gustavo Osvaldo Casta?o S Sookoian | 2013 | Gut2013，，9： | 2 |
| 8 | 湖泊中与微生物转化有关的氮素消涨显示文摘本研究观测了2个富营养化湖泊中微生物氮素的变化趋势,并与流入和流出湖泊的氮素消长情况进行了比较。浮游植物的初级生产力主要取决于再生氮素(尤其取决于铵态氮)的多少。浮游菌通过吸收溶解的游离氨基酸来满足其一年中大部分时间内对氮素的需求,并将相当大的一部分用于有机氮的再生。使硝酸盐变成分子态氮的微生物脱氮作用以及在沉积物中的永久性持留,是水生生态系统中氮素损耗的最主要途径。湖泊沉积物中的脱氮的估测方法是利用硝酸盐中的^(15)N作示踪物,然后测定所产生氮气的^(15)N/^(14)N比率而估算,并与以消涨计算得到的估测值进行比较,这两种方法得出了同水平估测值。由铵盐硝化作用得到的硝酸盐,马上就被作为脱氮作用的原料。脱氮作用每年的损耗量是外源氮素输入量的5%—25%,此值位于先前报道的变化范围的下限。湖泊沉积物中的氮素永久持留量是外源氮素输入量的14%—33%。 | Ingemar Ahlgren Tomas Waara Katarina Vrede Fred Srensson 马焕成 | 1994 | 人类环境杂志1994，23，6： | 2 |
| 9 | Determina- tion of SH- and SS-groups in some food pro- teins using Ellman's reagent 显示文摘 | Beveridge T Toma S J Nakai S | 1974 | Journal of Food Science1974，39，： | 1 |
| 10 | Insulin-like growth Factor- Ⅰ (IGF- Ⅰ ) and especially IGF- Ⅰ variants are anabolic in dexamethasone-treated rat显示文摘 | Tomas F M Knowles S E Owens P C | 1992 | Biochem J1992，282，： | 1 |
| 11 | Intrau-terine in- semination treatment in subfertility: an analysis of factors affecting outcome 显示文摘 | Nuojua-Huttunen S Tomas C Bloigu R | 1999 | Hum Reprod1999，14，3： | 1 |
| 12 | Facade solar collectors 显示文摘 | Tomas M Borivoj S | 2006 | Solar Ener- gy2006，80，： | 1 |
| 13 | Impact of metformin therapy on ovarian stimulation and outcome in 'coasted' patients with polycystic ovary syndrome undergoing in-vitro fertilization 显示文摘 | Stadtmauer L A Toma S K Riehl R M | 2002 | Reprod Biomed Online2002，5，2： | 1 |
| 14 | External ven- tricular drain insertion accuracy: is there a need for change in practice? 显示文摘 | Toma A K Camp S Watkins L D | 2009 | Neurosurgery2009，65，6： | 1 |
| 15 | Combined statin and niacin thera-py remodels the high - density lipoprotein proteome显示文摘 | Pattie S Green Tomas V etal | 2008 | Circula-tion2008，118，： | 1 |
| 16 | Transitory and permanent effects of electron beam irradiation on insulating materials 显示文摘 | Oproiu C Martin D Toma M S | 2000 | Nuclear Instruments and Methods in Physics Research B2000，669，2： | 1 |
| 17 | Amyotrophic cervical myelopathy in adolescence显示文摘 | Toma S Shiozawa Z | 1995 | Neurol Neurosurg Psychiatry1995，58，1： | 1 |
| 18 | Hospital outbreak caused by a carbapenem-resistant strain of Acinetobacter baumannii: patient prognosis and risk-factors for colonisation and infeetion 显示文摘 | Del Mar Tomas M Cartelle M Pertega S | 2005 | Clin Mierobiol Infect2005，11，7： | 1 |
| 19 | Sub150℃processed meso-superstructured perovskite solar cells with enhanced efficiency显示文摘 | KONRAD W MICHAEL S TOMAS L | 2014 | Energy&Environmental Science2014，7，3： | 1 |
| 20 | Morbidity after cesarean section显示文摘 | Visnovsky J Tomas S Danko J | 2003 | Ceska Gynekol2003，68，4： | 1 |
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