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| 1 | Thrombembolieprophylaxe auf deutschen Intensivstationen显示文摘 | Dr. P. Hilbert DEAA P. Teumer PD Dr. R. Stuttmann | 2008 | Der Anaesthesist2008，，3： | 1 |
| 2 | Recovery index, attentiveness and state of memory after xenon or isoflurane anaesthesia: a randomized controlled trial显示文摘 | Stuttmann R Jakubetz J Schuhz K | 2010 | BMC Anesthesio12010，7，： | 1 |
| 3 | The breast feeding mother and xenon and xenon anaesthesia:four case reports 显示文摘 | Stuttmann R Schafer C Hilbert P | 2010 | BMC Anesthesiol2010，10，： | 1 |
| 4 | Interaction between SGT1 and cytosolic/nuclear HSC70 chaperones regulates Arabidopsis immune responses 显示文摘 | Noel L D Cagna G Stuttmann J | 2007 | Plant Ce112007，19，： | 1 |
| 5 | Plasma atrial natriuretic peptide and brain natriuretic peptide are increased in septic shock: impact of interleukin-6 and sepsis-associated left ventricular dysfunction显示文摘 | Rochus Witthaut Christian Busch Peter Fraunberger Autar Walli Dietrich Seidel Günter Pilz Ralph Stuttmann Norbert Speichermann Ljifane Verner Karl Werdan | 2003 | Intensive Care Medicine2003，，10： | 1 |
| 6 | Trauma care in Germany:major differences in case fatality rates between centers显示文摘 | Hilbert P Lefering R Stuttmann R | 2010 | Dtsch Arztebl Int2010，107，26： | 1 |
| 7 | Recovery index, attentivemss and state of memory after xenon ~r isoflurane anaesthesia : a randomized controlled Irial 显示文摘 | Stuttmann R Jakubetz J Schullz K | 2010 | BMC Anesthesinl2010，10，： | 1 |
| 8 | Interaction between SGT1 and cytosolic/nuclear HSC70 chaperones regulates Arabidopsis immune responses 显示文摘 | NOEL L D CAGNA G STUTTMANN J | 2007 | The Plant Cell2007，19，12： | 1 |
| 9 | Trauma eare in Germany 显示文摘 | Hilbert P Lefering R Stuttmann R | 2010 | Dtsch Arztebl Int2010，107，26： | 1 |
| 10 | Assessing the depth of hypnosis of xenon anaesthesia with the EEG 显示文摘 | Stuttmann R Schultz A Kneif T | 2010 | Biomed Tech ( Berl )2010，55，2： | 1 |
| 11 | High-efficiencygenomeediting in plants mediated by a Cas9 gene containing multiple introns显示文摘The recent discovery of the mode of action of the CRISPR/Cas9 systemhas provided biologists with a useful tool for generating site-specific mutations in genes of interest.In plants,site-targeted mutations are usually obtained by the stable transformation of a Cas9 expression construct into the plant genome.The efficiency of introducing mutations in genes of interest can vary considerably depending on the specific features of the constructs,including the source and nature of the promoters and terminators used for the expression of the Cas9 gene and the guide RNA,and the sequence of the Cas9 nuclease itself.To optimize the efficiency of the Cas9 nuclease in generating mutations in target genes in Arabidopsis thaliana,we investigated several features of its nucleotide and/or amino acid sequence,including the codon usage,the number of nuclear localization signals(NLSs),and the presence or absence of introns.We found that the Cas9 gene codon usage had some effect on its activity and that two NLSs worked better than one.However,the highest efficiency of the constructs was achieved by the addition of 13 introns into the Cas9 coding sequence,which dramatically improved the editing efficiency of the constructs.None of the primary transformants obtained with a Cas9 gene lacking introns displayed a knockout mutant phenotype,whereas between 70%and 100%of the primary transformants generated with the intronized Cas9 gene displayed mutant phenotypes.The intronized Cas9 gene was also found to be effective in other plants such as Nicotiana benthamiana and Catharanthus roseus. | Ramona Grützner Patrick Martin Claudia Horn Samuel Mortensen Erin J.Cram Carolyn W.T.Lee-Parsons Johannes Stuttmann Sylvestre Marillonnet | 2021 | Plant Communications2021，2，2： | 0 |
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