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| 1 | 大熊猫电刺激采精及精液冷冻保存研究显示文摘对卧龙自然保护区大熊猫研究中心的 9只 5 5~ 16 5岁的雄性大熊猫进行了 17次电刺激采精 .其中 3只是能进行自然交配并繁殖了后代的种公兽 .比较研究了精液离心、不同稀释液和冷冻方法对大熊猫精液超低温冷冻保存后的活力、运动状态、精子和顶体形态的影响 .细管冷冻是 1种较好的超低温冷冻精液的方法 . | 黄炎 李德生 张和民 杜军 张贵权 王鹏彦 Jogayle Howard Rrbacca Spindler Barbara Durrant Mary Ann Olson | 2000 | 四川师范学院学报(自然科学版)2000，21，3： | 16 |
| 2 | 大熊猫精液超低温冷冻的比较显示文摘对卧龙自然保护区大熊猫研究中心的 9只雄性大熊猫电刺激采精 ,比较研究了稀释液中甘油含量、精液离心、不同稀释液和冷冻方法对大熊猫精液超低温冷冻保存后的活力、运动状态和顶体的影响。稀释液中甘油的含量为 4 %~ 5%较好 ,冻精解冻后的活力和顶体的正常率能保持鲜精的一半。离心和未离心的精液经超低温冷冻 ,解冻后的活力和运动状态都较接近。TEST和SFS两种稀释液的效果没有明显的差异。细管的冷冻过程较颗粒方便、快捷 ,时间容易控制 ,是一种较好的超低温冷冻精液的方法。 | 黄炎 李德生 杜军 王鹏彦 张和民 JoGayle Howard Rebecca Spindler | 2001 | 动物学杂志2001，36，2： | 4 |
| 3 | Paternal physical exercise modulates global DNA methylation status in the hippocampus of male rat offspring显示文摘It is widely known that maternal physical exercise is able to induce beneficial improvements in offspring cognition; however, the effects of paternal exercise have not been explored in detail. The present study was designed to evaluate the impact of paternal physical exercise on memory and learning, neuroplasticity and DNA methylation levels in the hippocampus of male offspring. Adult male Wistar rats were divided into two groups: sedentary or exercised fathers. The paternal preconception exercise protocol consisted of treadmill running, 20 minutes daily, 5 consecutive days per week for 22 days, while the mothers were not trained. After mating, paternal sperm was collected for global DNA methylation analysis. At postnatal day 53, the offspring were euthanized, and the hippocampus was dissected to measure cell survival by 5-bromo-2′-deoxiuridine and to determine the expression of synaptophysin, reelin, brain-derived neurotrophic factor and global DNA methylation levels. To measure spatial memory and learning changes in offspring, the Morris water maze paradigm was used. There was an improvement in spatial learning, as well as a significant decrease in hippocampal global DNA methylation levels in the offspring from exercised fathers compared with those from sedentary ones; however, no changes were observed in neuroplasticity biomarkers brain-derived neurotrophic factor, reelin and 5-bromo-2′-deoxiuridine. Finally, the global DNA methylation of paternal sperm was not significantly changed by physical exercise. These results suggest a link between paternal preconception physical activity and cognitive benefit, which may be associated with hippocampal epigenetic programming in male offspring. However, the biological mechanisms of this modulation remain unclear. | Christiano Spindler Ethiane Segabinazi Andre Luis Ferreira de Meireles Francele Valente Piazza Filipe Mega Gabriela dos Santos Salvalaggio Matilde Achaval Viviane Rostirola Elsner Simone Marcuzzo | 2019 | Neural Regeneration Research2019，14，3： | 3 |
| 4 | Determination of basal ileal endogenous losses and standardized ileal digestibility of amino acids in barley fed to growing pigs显示文摘Background: Basal ileal endogenous amino acid(AA) losses(IAA_(end)) and standardized ileal digestibility(SID) values of cereal grains, such as barley, are apparently underestimated when determined according to the nitrogen(N)-free method. Regression analysis between the dietary apparent ileal digestible content(c AID) and total crude protein(CP) and AA can be considered as alternative approach to obtain more accurate values for IAA_(end)and SID of AA in cereal grains.Methods: Eight hulled barley genotypes were used, with barley being the only source of CP and AA in the assay diets. The diets contained 95 % as-fed of these eight barley genotypes each, ranging in CP content between 109.1 and 123.8 g/kg dry matter(DM). Nine ileally T-cannulated barrows, average body weight(BW) 30 ± 2 kg, were allotted to a row-column design comprising eight periods with 6 d each and nine pigs. On d 5 and the night of d 6 of every period, ileal digesta were collected for a total of 12 h. The IAA_(end) and the SID were determined by linear regression analysis between c AID and total dietary CP and AA.Results: There exist linear relationships between cA ID and total CP and AA(P < 0.001). The IAA_(end) of CP, Lys, Met, Thr and Trp amounted to 35.34, 1.08, 0.25, 1.02 and 0.38 g/kg DM intake(DMI), respectively, which are greater compared to average IAA_(end) determined previously under N-free feeding conditions. The SID of CP, Lys, Met, Thr and Trp was 90,79, 85, 79 and 86 %, respectively, and was greater when compared to tabulated values. Moreover, these SID values were greater than those reported in literature, based on correction of apparent ileal digestibility(AID) of CP and AA for their IAA_(end)values. Summarized, the results of the present regression analysis indicate greater IAA_(end)in barley-based diets compared to those obtained by N-free feeding.Conclusions: For low-protein feed ingredients like barley the regression method may be preferred over correction of AID values for their IAA_(end)determined under N-free feeding conditions, as intercepts and slopes of the linear regression equations between cA ID and total dietary CP and AA provide direct estimates of IAA_(end)and SID of CP and AA in the presence of the assay feed ingredient. | Hanna Katharina Spindler Rainer Mosenthin Pia Rosenfelder Henry J?rgensen Knud Erik Bach Knudsen Meike Eklund | 2017 | Journal of Animal Science and Biotechnology2017，8，1： | 2 |
| 5 | Anxiety enhances the detrimental effect of depressive symptoms on health status following percutaneous coronary intervention显示文摘 | Susanne S. Pedersen Johan Denollet Helle Spindler Andrew T.L. Ong Patrick W. Serruys Ruud A.M. Erdman Ron T. van Domburg | 2006 | Journal of Psychosomatic Research2006，，6： | 2 |
| 6 | 大熊猫精液的研究显示文摘在 1999年 3月至 2 0 0 0年 6月 ,对卧龙自然保护区大熊猫研究中心的 9只 5 .5~ 16 .5岁的雄性大熊猫进行了 17次电刺激采精 ,9只大熊猫的平均体重为 10 8.1± 15 kg,睾丸体积为 2 89.4± 10 8.6 cm3,刺激电压 2~ 8V,射精电压 4~ 8V,平均每次采精量为 3.5± 1.91ml,精子密度为 995 .2± 782 .6× 10 6 个 / ml,每次采精总量为 390 7± 2 981.7× 10 6 个 ,精液平均活力为 6 9.1± 2 8.9% ,活率为 79.6± 2 3.1% ,运动状态为 2 .7± 1.1,畸形率为 39.4± 32 .2 % ,平均顶体正常率为 85 .8± 31.2 % ,p H值为 7.9± 0 .4。其中 3只是能进行自然交配并繁殖了后代的种公兽 ,其体重为 12 2 .7± 7.1kg,睾丸体积为 381.7± 6 4.8cm3,精液活力为 77.3± 5 .8% ,运动状态为 3.0± 0 .5 ,精子畸形率为 18.0± 9.6 % ,顶体正常率为 92 .7± 1.5 % ,p H值为 8.0±0 .1。大熊猫异常精子主要有 :顶体异常 ,卷尾 ,中段弯曲含胞浆小滴 ,中段弯曲无胞浆小滴 ,尾弯曲含胞浆小滴 ,尾弯曲无胞浆小滴 ,近端含胞浆小滴 ,远端含胞浆小滴 8种。在 2月~ 6月都能通过电刺激采集到精液 ,在 4月份每次产精量最高 ,这正好与在这期间发情和交配的大熊猫数量最多相吻合。 | 黄炎 李德生 张和民 杜军 张贵权 王鹏彦 JoGayle Howard Rebecca Spindler | 2001 | 中国兽医杂志2001，37，6： | 2 |
| 7 | Postperi cardiotomy syndrome and cardiac tamponade as a 1 ate complication after pacemaker implantation显示文摘 | Spindler M Burrows G Kowallik P | 2001 | Pacing Clin Elect rophysiol2001，24，9： | 1 |
| 8 | Enhancement of latent fingermarks on non-porous surfaces using anti-l-amino acid antibodies conjugated to gold nanoparticles 显示文摘 | Spindler X Hofstetter O Mcdonagh AM | 2011 | Chem Commun2011，47，19： | 1 |
| 9 | The prognostic value of KRAS mutated plasma DNA in advanced non-small cell lung cancer显示文摘 | Nygaard AD Garm Spindler KL Pallisgaard N | 2013 | Lung Can- cer2013，79，3： | 1 |
| 10 | Protective endogenous cyclic adenosine 59-monophosphate signaling triggered by pemphigus autoantibodies显示文摘 | Spindler V Vielmuth F Schmidt E | 2010 | J Immunol2010，185，11： | 1 |
| 11 | Role of GTPases in control of microvascular permeability 显示文摘 | Spindler V Schlegel N Waschke J | 2010 | Cardiovasc Res2010，87，2： | 1 |
| 12 | Visualization of latent fingermarks using an aptamer-based reagent 显示文摘 | Wood M Maynard P Spindler X | 2012 | Angewandte Chemie lnt Ed2012，51，12： | 1 |
| 13 | Anxiety enhances the cletrimenta effect of depressive symptomson teaeh status following percutaneous coronary intervention显示文摘 | Pedersen S Denollet J Spindler H | 2006 | J Psychosom Res2006，61，6： | 1 |
| 14 | Simultaneous saccharification and fermentation of several lignocellulosic feedstocks to fuel ethanol显示文摘 | Wyman C E Spindler D D Grohmann K | 1992 | Biomass and Bioenergy1992，3，5： | 1 |
| 15 | Characterization of chitinase from the brine shrimp Artemia显示文摘 | Funke B Spindler K D | 1989 | Comp Biochem Physiol1989，94，4： | 1 |
| 16 | Pharmacologic agents in fracture healing显示文摘 | Koester MC Spindler KP | 2006 | Ciin Sports IVied2006，25，1： | 1 |
| 17 | Efficacy and safety of ocrelizumab in active proliferative lupus nephritis:Results from a randomized,double-blind,phase Ⅲ study显示文摘 | Mysler EF Spindler AJ Guzman R | | 0，，： | 1 |
| 18 | Prognostic score systems and community-acquired bacteraemic pneumococeal pneumonia 显示文摘 | Spindler C Ortqvist A | 2006 | Eur Respir J2006，28，4： | 1 |
| 19 | The biomechanical response to doses of TGF-beta 2 in the healing rabbit medial collateral ligament 显示文摘 | Spindler KP Murray MM Detwiler KB | 2003 | J Orthop Res2003，21，2： | 1 |
| 20 | Amino-acid transport by heterodimers of4F2hc/CD98 and members of a permease family显示文摘 | MASTROBERARDINO L SPINDLER B PFEIFFER R | 1998 | Nature1998，395，17： | 1 |
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