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| 1 | Total saponins of Panax ginseng effects on proliferation and differentiation of human embryonic neural stem cells and in a Parkinson's disease mouse model显示文摘BACKGROUND:Total saponins of Panax ginseng(TSPG) exhibits neuroprotection against Parkinson's disease in the substantia nigra. OBJECTIVE:To investigate the effects of TSPG on human embryonic neural stem cells(NSCs) proliferation and differentiation into dopaminergic neurons using in vitro studies,and to observe NSC differentiation in a mouse model of Parkinson's disease,as well as behavioral changes before and after transplantation. DESIGN,TIME AND SETTING:In vitro neural cell biology trial and in vivo randomized,controlled animal trial were performed at the Institute of Basic Medical Sciences,Chongqing Medical University between September 2004 and December 2007. MATERIALS:TSPG(purity>95%) was isolated,extracted,and identified by Chongqing Academy of Chinese Materia Medica.Recombinant human basic fibroblast growth factor(bFGF) and recombinant human epidermal growth factor(EGF) were purchased from PeproTech,USA.A total of 25 C57/BL6J mice,aged 18-20 weeks were included.Twenty were used to establish a Parkinson's disease model with i.p.injection of MPTP(1-methyl-4-phenyl-1,2,3, 6-tetrahydropyridine) and TSPG alone or combined with interleukin-1(IL-1)-treated NSCs prior to transplantation into the corpus striatum.The remaining five mice were pretreated for 3 days with TSPG prior to MPTP injection,serving as the TSPG prevention group. METHODS:Primary NSCs were isolated,cultured and purified from embryonic cerebral cortex. Immunocytochemistry was employed to detect specific antigen expression in the NSCs.In vitro experiment:(1) to induce proliferation,NSCs were treated with TSPG,EGF+bFGF,or TSPG+EGF+bFGF,respectively;(2) to induce dopaminergic neuronal differentiation,NSCs were treated with TSPG,IL-1,or TSPG+IL-1,respectively. MAIN OUTCOME MEASURES:In vitro experiment:the effects of TSPG on NSCs proliferation were evaluated with flow cytometry and MTT assay.Tyrosine hydroxylase expression was determined by immunocytochemistry assay to observe effects of TSPG on dopaminergic neuronal differentiation.In vivo experiment:differentiation of grafted NSCs in the mouse brain was determined by immunohistochemical staining.Behavioral changes were evaluated by spontaneous activity frequency,memory function,and score of paralysis agitans. RESULTS:(1) NSCs were cultured and passaged for more than three passages. Immunocytochemistry revealed positive nestin staining,as well as neurofilament protein and glial fibrillary acidic protein.(2) TSPG significantly increased NSC proliferation,in particular when combined with EGF and bFGF,which was twice as effective as FGF or bFGF alone.TSPG also induced dopaminergic differentiation in NSCs,in particular when TSPG was added together with IL-1,resulting in an effect five times greater than that of IL-1 alone.(3) At day 30 following transplantation,most NSCs in the TSPG prevention group differentiated into dopaminergic neurons,and the scores of paralysis agitans,spontaneous activity,and memory function were significantly increased compared with TSPG alone or TSPG+IL-1 groups(P<0.05). CONCLUSION:TSPG stimulated NSC proliferation,in particular when combined with FGF and bFGF.TSPG significantly induced dopaminergic neuronal differentiation of NSCs,and the effect was greater when combined with IL-1.In addition,TSPG greatly improved behavior in the Parkinson's disease mouse model following NSC transplantation.Following NSC transplantation, TSPG pretreatment exhibited superior efficacy over either TSPG alone or TSPG in combination with IL-1,in terms of behavioral improvements in the Parkinson's disease mouse model. | Yingbo Li Shali Wang | 2009 | Neural Regeneration Research2009，4，3： | 3 |
| 2 | Maternal serum adiponectin multimersin gestational diabetes显示文摘 | Shali MT Roberto R Edi V | 2009 | Perinat Med2009，37，6： | 1 |
| 3 | 显示文摘 | HE Shali ZHAO Yifang ZHU Zhiwei | 2006 | Talanta2006，69，： | 1 |
| 4 | Insulin sensitivity in late gestation and early postpartum period:the role of circulating maternal adipokines 显示文摘 | SHALI MAZAKI-TOVI HANNAH KANETY CLARA P- ARIENTE | 2011 | Gynecological Endocrinology2011，27，9： | 1 |
| 5 | A prospective cohort study of the value of maternal plasma concentrations of angiogenic and anti-angiogenic factors in early pregnancy and midtrimester in the identification of patients destined to develop preeclampsia显示文摘 | Juan Pedro Kusanovic Roberto Romero Tinnakorn Chaiworapongsa Offer Erez Pooja Mittal Edi Vaisbuch Shali Mazaki-Tovi Francesca Gotsch Samuel S. Edwin Ricardo Gomez Lami Yeo Agustin Conde-Agudelo Sonia S. Hassan | 2009 | Journal of Maternal-Fetal and Neonatal Medicine2009，，11： | 1 |
| 6 | Effects of Propofol on the Outcomes of Rats with gepsis显示文摘 | HONG GUANG BAO SHALI | 2011 | Journal of gurglcal Re- search2011，168，1： | 1 |
| 7 | Spermatogenesis following syngeneictesticular transplantation in Balb/c mice 显示文摘 | Ma Pengpeng Ge Yehua Wang Shali | 2004 | Reproduction2004，128，： | 1 |
| 8 | Effect of TSPG on the proliferation and differentiation of human embryonic neural stem cells and the trans- plantation of nSCs into mice with parkinson's disease显示文摘 | Li Yingbo Wang Shali | 2009 | Neural Regen Res2009，4，3： | 1 |
| 9 | A subset of patients destined to develop spontaneous preterm labor has an abnormal angiogenic/anti-angiogenic profile in maternal plasma: Evidence in support of pathophysiologic heterogeneity of preterm labor derived from a longitudinal study显示文摘 | Tinnakorn Chaiworapongsa Roberto Romero Adi Tarca Juan Pedro Kusanovic Pooja Mittal Sun K. Kim Francesca Gotsch Offer Erez Edi Vaisbuch Shali Mazaki-Tovi Percy Pacora Giovanna Ogge Zhong Dong Chong J. Kim Lami Yeo Sonia S. Hassan | 2009 | Journal of Maternal-Fetal and Neonatal Medicine2009，，12： | 1 |
| 10 | DeterminationofPhysiologicalIndexesWoolmeatSheepinInnerMongolia显示文摘 | SHALi(沙里) CAIHongyong(蔡弘永) Jirigela(吉日戈托) | 1982 | 内蒙古农牧学院学报1982，，1： | 1 |
| 11 | Visfatin/Pre-B cell colony-enhancing factor in amniotic fluid in normal pregnancy, spontaneous labor at term, preterm labor and prelabor rupture of membranes: an association with subclinical intrauterine infection in preterm parturition显示文摘 | Shali Mazaki-Tovi Roberto Romero Juan Pedro Kusanovic Offer Erez Francesca Gotsch Pooja Mittal Nandor Gabor Than Chia-Lang Nhan-Chang Neil Hamill Edi Vaisbuch Tinnakorn Chaiworapongsa Samuel S. Edwin Jyh Kae Nien Ricardo Gomez Jimmy Espinoza Claire Kendal | 2008 | Journal of Perinatal Medicine2008，，6： | 1 |
| 12 | Visfatin/Pre-B Cell Colony-Enhancing Factor in Amniotic Fluid in Normal Pregnancy,Spontaneous Labor at Term,Preterm Labor and Prelabor Rupture of Membranes:an Association with Subclinical Intrauterine Infection in Preterm Parturition显示文摘 | Shali MT Roberto R Juan PK | 2008 | J Perinat Med2008，36，6： | 1 |
| 13 | C-fiber reinforced hydroxyapatite bioceramics 显示文摘 | Zhang Yujun Tan Shali Yin Yansheng | 2003 | Ceramics International2003，29，： | 1 |
| 14 | A prospective cohort study of the value of maternal plasma concentrations of angiogenic and anti-angiogenic factors in early pregnancy and midtrimester in the identification of patients destined to develop preeclampsia显示文摘 | Juan Pedro Kusanovic Roberto Romero Tinnakorn Chaiworapongsa Offer Erez Pooja Mittal Edi Vaisbuch Shali Mazaki-Tovi Francesca Gotsch Samuel S. Edwin Ricardo Gomez Lami Yeo Agustin Conde-Agudelo Sonia S. Hassan | 2009 | Journal of Maternal-Fetal and Neonatal Medicine2009，，11： | 1 |
| 15 | Diabetes-induced Myocardial Structural Changes: Role of Endothelin-1 and its Receptors显示文摘 | Shali Chen Terry Evans Kallol Mukherjee Morris Karmazyn Subrata Chakrabarti | 2000 | Journal of Molecular and Cellular Cardiology2000，，9： | 1 |
| 16 | Modeling and verification of reactive systems using Rebeca显示文摘 | Sirjani M Movaghar A Shali A | 2004 | Fundamenta Informatica2004，63，： | 1 |
| 17 | The role of T lymphoma invasionand metastasis inducing protein 1 in early pregnancy in mice显示文摘 | Hailan M Shali W Wang Q Y | 2008 | Mol Hum Reprod2008，14，10： | 1 |
| 18 | Genetic variation in PPARG encoding Peroxisome proliferator activated receptor-γ associated with carotid atherosclerosis显示文摘 | Khalid Z Al- Shali Andrew A | 2004 | Stroke2004，35，： | 1 |
| 19 | Cell-delivery therapeutics for liver regeneration显示文摘 | Wenxia Zhang Lisa Tucker-Kellogg B.C. Narmada Lakshmi Venkatraman Shi Chang Yin Lu Nancy Tan Jacob K. White Ruirui Jia Sourav Saha Bhowmick Shali Shen C. Forbes Dewey Hanry Yu | 2010 | Advanced Drug Delivery Reviews2010，，7： | 1 |
| 20 | Exome sequencing identifies rare mutations of LDLR and QTRT1 conferring risk for early-onset coronary artery disease in Chinese显示文摘Despite the advances made over the past decades,the known loci for coronary artery disease(CAD)still only explain<20%of the genetic variation in risk[1,2].The known loci with the strongest effects usually confer a 20%-37%increased CAD risk and the most loci modulate risk by≤10%[1].While most of the current data are from European populations,the use of trans-ethnic analyses could be helpful to identify additional loci.Genetic inheritance may impose a high burden on early-onset coronary artery disease(EOCAD). | Kang Yao Yuxiang Dai Juan Shen Yi Wang Huanjie Yang Runda Wu Qijun Liao Hongyi Wu Xiaodong Fang Shalaimaiti Shali Lili Xu Meng Hao Chenhao Lin Zhonghan Sun Yilian Liu Mengxin Li Zhen Wang Qiang Gao Shuning Zhang Chenguang Li Wei Gao Lei Ge Yunzeng Zou Aijun Sun Juying Qian Li Jin Shangyu Hong Yan Zheng Junbo Ge | 2022 | National Science Review2022，9，8： | 1 |
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