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| 1 | The intracellular mechanism of alpha-fetoprotein promoting the proliferation of NIH 3T3 cells显示文摘AIM The existence and properties of alpha-fetoprotein (AFP) receptor on the surface of NIH 3T3 cells and the effects of AFP on cellular signal transduction pathway were investigated. METHODS The effect of AFP on the proliferation of NIH 3T3 cells was measured by incorporation of 3H-TdR. Receptor-binding assay of 125I-AFP was performed to detect the properties of AFP receptor in NIH 3T3 cells. The influences of AFP on the [cAMP]i and the activities of protein kinase A (PKA) were determined. Western blot was used to detect the change of K-ras P21 protein expression. RESULTS The proliferation of NIH 3T3 cells treated with 0-80 mg/L of AFP was significantly enhanced. The Scatchard analysis indicated that there were two classes of binding sites with KD of 2.722×10-9M (Bmax=12810 sites per cell) and 8.931× 10-SM (Bmax=l19700 sites per cell) respectively. In the presence of AFP (20 mg/L), the content of cAMP and activities of PKA were significantly elevated . The level of K-ras P21 protein was upregulated by AFP at the concentration of 20 mg/L. The monoclonal antibody against AFP could reverse the effects of AFP on the cAMP content, PKA activity and the expression of K-ras p21 gene. CONCLUSION The effect of AFP on the cell proliferation was achieved by binding its receptor to trigger the signal transduction pathway of cAMP-PKA and alter the expression of K- ras p21 gene. | MENG SEN LI, PING FENG LI, FBI YI YANG, SHI PENG HE, Guo GUANG DU, GANG LI1 Department of Biochemistry and Molecular Biology, 2 Department of Biophysics, Health Science Center, Peking University, Beijing 100083, China | 2002 | Cell Research2002，12，2： | 27 |
| 2 | Clinical Characteristics of Patients with Diabetic Nephropathy on Maintenance Hemodialysis: A Multicenter Cross-sectional Survey in Anhui Province, Eastern China显示文摘 | Hu Chen De-Guang Wang Liang Yuan Gui-Ling Liu Heng-Jie He Juan Wang Sen Zhang Li Hao | 2016 | Chinese Medical Journal2016，，11： | 24 |
| 3 | Olivine-norite rock detected by the lunar rover Yutu-2 likely crystallized from the SPA-impact melt pool显示文摘Chang’E-4 landed in the South Pole-Aitken(SPA)basin,providing a unique chance to probe the composition of the lunar interior.Its landing site is located on ejecta strips in Von Karm´an crater that´possibly originate from the neighboring Finsen crater.A surface rock and the lunar regolith at 10 sites along the rover Yutu-2 track were measured by the onboard Visible and Near-Infrared Imaging Spectrometer in the first three lunar days of mission operations.In situ spectra of the regolith have peak band positions at 1 and 2μm,similar to the spectral data of Finsen ejecta from the Moon Mineralogy Mapper,which confirms that the regolith’s composition of the landing area is mostly similar to that of Finsen ejecta.The rock spectrum shows similar band peak positions,but stronger absorptions,suggesting relatively fresh exposure.The rock may consist of 38.1±5.4%low-Ca pyroxene,13.9±5.1%olivine and 48.0±3.1%plagioclase,referred to as olivine-norite.The plagioclase-abundant and olivine-poor modal composition of the rock is inconsistent with the origin of the mantle,but representative of the lunar lower crust.Alternatively,the rock crystallized from the impact-derived melt pool formed by the SPA-impact event via mixing the lunar crust and mantle materials.This scenario is consistent with fast-cooling thermal conditions of a shallow melt pool,indicated by the fine to medium-sized texture(<3 mm)of the rock and the SPA-impact melting model[Icarus 2012;220:730–43]. | Honglei Lin Zhiping He Wei Yang Yangting Lin Rui Xu Chi Zhang Meng-Hua Zhu Rui Chang Jinhai Zhang Chunlai Li Hongyu Lin Yang Liu Sheng Gou Yong Wei Sen Hu Changbin Xue Jianfeng Yang Jie Zhong Xiaohui Fu Weixing Wan Yongliao Zou | 2020 | National Science Review2020，7，5： | 9 |
| 4 | Expression analysis of ETS1 gene in peripheral blood mononuclear cells with systemic lupus erythematosus by real-time reverse transcription PCR显示文摘 | Li Yun Sun Liang-dan Lu Wen-sheng Hu Wen-long Gao Jin-ping Cheng Yi-lin Yu Ze-ying Yao Sha He Cai-feng Liu Jian-lan Cui Yong Yang Sen | 2010 | Chinese Medical Journal2010，，16： | 8 |
| 5 | Facilitating Lithium-Ion Diffusion in Layered Cathode Materials by Introducing Li^(+)/Ni^(2+) Antisite Defects for High-Rate Li-Ion Batteries显示文摘Li^(+)/Ni^(2+) antisite defects mainly resulting from their similar ionic radii in the layered nickel-rich cathode materials belong to one of cation disordering scenarios.They are commonly considered harmful to the electrochemical properties,so a minimum degree of cation disordering is usually desired.However,this study indicates that LiNi_(0.8)Co_(0.15)Al_(0.05)O_(2)as the key material for Tesla batteries possesses the highest rate capability when there is a minor degree(2.3%)of Li^(+)/Ni^(2+) antisite defects existing in its layered structure.By combining a theoretical calculation,the improvement mechanism is attributed to two effects to decrease the activation barrier for lithium migration:(1)the anchoring of a low fraction of high-valence Ni^(2+) ions in the Li slab pushes uphill the nearest Li^(+)ions and(2)the same fraction of low-valence Li^(+) ions in the Ni slab weakens the repulsive interaction to the Li^(+) ions at the saddle point. | Zhongfeng Tang Sen Wang Jiaying Liao Shuo Wang Xiaodong He Bicai Pan Haiyan He Chunhua Chen | 2019 | Research2019，，1： | 8 |
| 6 | DOA Estimation Based on Sparse Representation of the Fractional Lower Order Statistics in Impulsive Noise显示文摘This paper is mainly to deal with the problem of direction of arrival(DOA) estimations of multiple narrow-band sources impinging on a uniform linear array under impulsive noise environments. By modeling the impulsive noise as α-stable distribution, new methods which combine the sparse signal representation technique and fractional lower order statistics theory are proposed. In the new algorithms, the fractional lower order statistics vectors of the array output signal are sparsely represented on an overcomplete basis and the DOAs can be effectively estimated by searching the sparsest coefficients. To enhance the robustness performance of the proposed algorithms,the improved algorithms are advanced by eliminating the fractional lower order statistics of the noise from the fractional lower order statistics vector of the array output through a linear transformation. Simulation results have shown the effectiveness of the proposed methods for a wide range of highly impulsive environments. | Sen Li Rongxi He Bin Lin Fei Sun | 2018 | IEEE/CAA Journal of Automatica Sinica2018，5，4： | 7 |
| 7 | FRUCTOKINASE-LIKE PROTEIN 1 interacts with TRXz to regulate chloroplast development in rice显示文摘Chloroplast genes are transcribed by the plastidencoded RNA polymerase(PEP) or nucleus-encoded RNA polymerase. FRUCTOKINASE-LIKE PROTEINS(FLNs) are phosphofructokinase-B(Pfk B)-type carbohydrate kinases that act as part of the PEP complex; however, the molecular mechanisms underlying FLN activity in rice remain elusive.Previously, we identified and characterized a heat-stress sensitive albino(hsa_1) mutant in rice. Map-based cloning revealed that HSA_1 encodes a putative OsFLN_2. Here, we further demonstrated that knockdown or knockout of the OsFLN_1, a close homolog of HSA_1/OsFLN_2, considerably inhibits chloroplast biogenesis and the fln_1 knockout mutants, created by clustered regularly interspaced short palindromic repeats(CRISPR) and CRISPR-associate protein_9, exhibit severe albino phenotype and seedling lethality. Moreover, OsFLN_1 localizes to the chloroplast.Yeast two-hybrid, pull-down and bimolecular fluorescencecomplementation experiments revealed that OsFLN_1 and HSA_1/OsFLN_2 interact with THIOREDOXINZ(OsTRXz) to regulate chloroplast development. In agreement with this,knockout of OsTRXz resulted in a similar albino and seedling lethality phenotype to that of the fln_1 mutants. Quantitative reverse transcription polymerase chain reaction and immunoblot analysis revealed that the transcription and translation of PEP-dependent genes were strongly inhibited in fln_1 and trxz mutants, indicating that loss of OsFLN_1, HSA_1/OsFLN_2, or OsTRXz function perturbs the stability of the transcriptionally active chromosome complex and PEP activity. These results show that OsFLN_1 and HSA_1/OsFLN_2 contribute to chloroplast biogenesis and plant growth. | Lei He Sen Zhang Zhennan Qiu Juan Zhao Wendan Nie Haiyan Lin Zhengge Zhu Dali Zeng Qian Qian Li Zhu | 2018 | Journal of Integrative Plant Biology2018，60，2： | 6 |
| 8 | Improved production of docosahexaenoic acid in batch fermentation by newly-isolated strains of Schizochytrium sp.and Thraustochytriidae sp.through bioprocess optimization显示文摘Thraustochytrids,rich in docosahexaenoic acid(DHA,C22:6u3),represent a potential source of dietary fatty acids.Yet,the effect of culture conditions on growth and fatty acid composition vary widely among different thraustochytrid strains.Two different thraustochytrid strains,Schizochytrium sp.PKU#Mn4 and Thraustochytriidae sp.PKU#Mn16 were studied for their growth and DHA production characteristics under various culture conditions.Although they exhibited similar fatty acid profiles,PKU#Mn4 seemed a good candidate for industrial DHA fermentation while PKU#Mn16 displayed growth tolerance to a wide range of process conditions.Relative DHA content of 48.5%and 49.2%(relative to total fatty acids),respectively,were achieved on glycerol under their optimal flask culture conditions.Maximum DHA yield(Yp/x)of 21.0%and 18.9%and productivity of 27.6 mg/L-h and 31.9 mg/L-h were obtained,respectively,in 5-L bioreactor fermentation operated with optimal conditions and dual oxygen control strategy.A 3.4-and 2.8-fold improvement of DHA production(g/L),respectively,was achieved in this study.Overall,our study provides the potential of two thraustochytrid strains and their culture conditions for efficient production of DHA-rich oil. | Qiuzhen Wang Huike Ye Biswarup Sen Yunxuan Xie Yaodong He Sunghoon Park Guangyi Wang | 2018 | Synthetic and Systems Biotechnology2018，3，2： | 4 |
| 9 | Effect of stellate block on vasomotor factor, vascular endothelial nitricoxide synthase and pulmonary arterial pressure in rabbits with hypoxic pulmonary artery hypertension显示文摘BACKGROUND: At present, inhalation of nitrogen monoxidum (NO) or other angiotenic is widely used to cure hypoxic pulmonary artery hypertension. In addition, recent researches demonstrate that postganglionic fiber of stellate ganglion can regulate contents of blood vessel endothelium-calcitonin gene-related peptide (BVE-CGRP) and nitricoxide synthase (NOS) in lung tissue. Therefore, stellate ganglion which is blocked with the local anesthetic may cause therapeutic effects on hypoxic pulmonary artery hypertension. OBJECTIVE: To observe the effects of stellate block on calcitonin gene-related peptide (CGRP) of vasodilation factors, prostacyclin, endothelin-1 of vasoconstriction factors, thromboxan, blood vessel endothelium-nitricoxide synthase (BVE-NOS) and mean arterial pressure of lung tissue in rabbits with hypoxic pulmonary artery hypertension. DESIGN: Randomly controlled animal study. SETTING: Neurological Institute of Taihe Hospital Affiliated to Yunyang Medical College. MATERIALS: A total of 24 adult Japanese rabbits of both genders and weighing 2.3–2.6 kg were provided by Animal Experimental Center of Hubei Academy of Medical Science. SP kit was provided by Beijing Zhongshan Biotechnology Co., Ltd.; moreover, kits of endothelin-1, CGRP, prostacyclin and thromboxan were provided by Radioimmunity Institute, Scientific and Technological Developing Center, General Hospital of Chinese PLA, and color image analytical system (Leica-Q500IW) was made in Germany. METHODS: The experiment was carried out in the Neurological Institute of Taihe Hospital affiliated to Yunyang Medical College from February to December 2002. ① Rabbits were performed with aseptic manipulation to exposure left stellate ganglion and then it was put in epidural catheter for 1 week. In addition, one end of epidural catheter was fixed near by stellate ganglion and the other end was fixed through dorsal neck. All rabbits were randomly divided into 4 groups, including normal control group, stellate block group, hypoxia group and hypoxia + stellate block group, with 6 in each group. Rabbits in the normal control group were perfused with saline through epidural catheter with 0.5 mL once for three times per day and 3 successive days in total; in addition, rabbits in the stellate block group were perfused with 2.5 g/L bupivacaine through epidural catheter with 0.5 mL once for three times per day and 3 successive days in total. Rabbits in the hypoxia group were used to establish hypoxic pulmonary artery hypertension models. That was to say, the experimental rabbits were put in hypoxic box (containing sodalime and calcium chloride to absorb CO2 and water) and given various flows of oxygen and nitrogen through the two lateral wells simultaneously. And then, oxygen was monitored with oxygen-concentration monitoring device to control the concentration in (10±2)% for 8 hours per day and 2 successive weeks in total. Rabbits in the hypoxia + stellate block group were used to establish hypoxia models as the same as those in the hypoxia group. Two weeks later, 2.5 g/L bupivacaine was pushed into epidural catheter with 0.5 mL once for three times per day and 3 successive days in total. Breast was directly opened to measure mean pulmonary artery pressure. ② 6 mL blood was collected through pulmonary arterial duct to measure levels of plasma CGRP, prostacyclin, endothelin-1 and thromboxane with radio-immunity technique; meanwhile, immunohistochemical staining was used to observe the changes of BVE-NOS content of the experimental rabbits in all groups. MAIN OUTCOME MEASURES: Changes of CGRP, prostacyclin, endothelin-1 and thromboxane and BVE-NOS. RESULTS: A total of 24 experimental rabbits were involved in the final analysis. ① As compared withthose in the normal control group, hypoxic pulmonary artery hypertension of the experimental rabbits was higher in the hypoxia group and hypoxia + stellate block group after hypoxia [(3.84±0.30), (3.16±0.45), (2.60±0.27) kPa, P < 0.05, 0.01]; CGRP was lower [(68.20±8.78), (108.24±14.35), (130.25±22.70) ng/L, P < 0.05, 0.01]; prostacyclin was lower [(94.45±10.68), (98.77±12.31), (155.27±20.67) ng/L, P < 0.01]; endothelin-1 was higher [(184.74±29.66), (115.27±13.62), (98.20±11.52), ng/L, P < 0.05, 0.01]; thromboxan was higher [(226.27±30.46), (207.67±27.32), (124.25±16.89) ng/L, P < 0.01]. As compared with that in hypoxia group, hypoxic pulmonary artery hypertension was decreased in hypoxia + stellate block group (P < 0.05), CGRP was increased (P < 0.01), and endothelin-1 was decreased remarkably (P < 0.05). ② Level of BVE-NOS of the experimental rabbits was higher in stellate block group, hypoxia group and hypoxia + stellate block group than that in the normal control group [(0.25±0.06), (0.27±0.07), (0.46± 0.12), (0.14±0.03), P < 0.05], and NOS level was higher in the hypoxia + stellate block group than that in hypoxia group (P < 0.05). CONCLUSION: Mean arterial pressure is decreased in rabbits with hypoxic pulmonary artery hypertension after stellate block and level of endothelin-1 is also decreased; however, levels of CGRP and NOS are increased respectively. | Shunhou He Qing Li Sen Chen Qingxiu Wang | 2007 | Neural Regeneration Research2007，2，3： | 3 |
| 10 | The scoring system for patients with severe sepsis after orthotopic liver transplantation显示文摘BACKGROUND: Because of the complicated pathological features after liver transplantation, severe sepsis is difficult to treat and often leads to death. This study was undertaken to analyze the role of orthotopic liver transplantation (OLT) in patients with severe sepsis and to evaluate the effect of the scoring system. METHODS: Fifty-six patients conformed to the inclusion criteria. They were divided into two groups: non-OLT group (group A) and OLT group (group B). Besides the general data of the patients, the surveillance of blood lactate, the number of failed organs, acute physiology and chronic health evaluationⅡ(APACHEⅡ) and mutiple organ dysfunction score (MODS) were evaluated at the 1st, 3rd and 7th day after OLT. RESULTS: The mortality during hospitalization was 30% in the non-OLT group and 57.6% in the other group. The level of blood lactate at the 1st day of OLT increased more significantly in the OLT group than in the non-OLT group (P<0.01). It was decreased but higher than that in the non-OLT group in the seven days after OLT. The number of failed organs in the OLT group was greater than that in the non-OLT group (P<0.01). The continuous score of APACHEⅡwas not significantly different in the two groups. But the continuous MODS in the OLT group was higher than that in the non-OLT group (P<0.01), which was consistent with the number of failed organs. CONCLUSIONS: The persistently higher level of blood lactate during 7 days may be a dependent risk factor. Immunosuppression may be another risk factor for OLT patients. The mortality of OLT in patients with severe sepsis in 28 days is almost double that in non-OLT patients. The MODS score is better than the APACHEⅡscore in the assessment of organ failure in OLT patients with severe sepsis. The standard scoring system could be improved or a new scoring system that includes the blood lactate score should be established for liver transplantation. | Shun-Wei Huang, Xiang-Dong Guan, Xiao-Shun He, Juan Chen and Bin Ouyang Department of Surgical Intensive Care Unit ,and Department of Transplantation Surgery,First Affiliated Hospital, Sun Yat- Sen University, Guangzhou 510800, China | 2006 | Hepatobiliary & Pancreatic Diseases International2006，5，3： | 3 |
| 11 | Influence of N doping and the functional groups of graphene on a RGO/TiO2 composite photocatalyst显示文摘Reduced graphene oxide(RGO)assisted TiO2 composite photocatalysts have attracted increased attention because of their high photocatalytic performance under both UV-and visible-light irradiation.However,the corresponding properties under the long wavelength irradiation is far from expected.In this study,a series of RGO samples with well-designed N impurity and optimized surface functional groups are prepared and employed during hybridization with TiO2(named N-RGO/TiO2).The coupling between the doped N atoms and surface functional groups is analyzed based on the resulting photocatalytic performances,and the specific mechanism is revealed by the EPR,IR and lifetime of the photoinduced electrons.After comparing the corresponding performances of the RGO/TiO2 and RGO/N-TiO2 specimens,the results show that the surface functional groups of the RGO serve as a precondition to achieveπ-d coupling between the graphene basal plane and TiO2 nanoparticles,while N atoms in the RGO enhance the resulting photoactivity under a long wavelength range(>510 nm).The findings provide a potential approach to promote the photocatalytic properties of graphene-based composite photocatalysts with the low-energy incident photons. | TANG Bo HE YanFeng ZHANG ZhenYu WANG ZhengWei JI Li MA TingTing LI Sen DAI YuanZhe ZHANG GuoLiang | 2020 | Science China(Technological Sciences)2020，63，6： | 3 |
| 12 | A field-effect approach to directly profiling the localized states in monolayer MoS2显示文摘A fundamental understanding of the charge transport mechanism in two-dimensional semiconductors(e.g., MoS2) is crucial for fully exploring their potential in electronic and optoelectronic devices. By using monolayer graphene as the barrier-free contact to MoS2, we show that the field-modulated conductivity can be used to probe the electronic structure of the localized states. A series of regularly distributed plateaus were observed in the gate-dependent transfer curves. Calculations based on the variable-range hopping theory indicate that such plateaus can be attributed to the discrete localized states near mobility edge. This method provides an effective approach to directly profiling the localized states in conduction channel with an ultrahigh resolution up to 1 meV. | Hao Wu Yuan Liu Zeyu Deng Hung-Chieh Cheng Dehui Li Jian Guo Qiyuan He Sen Yang Mengning Ding Yun-Chiao Huang Chen Wang Yu Huang Xiangfeng Duan | 2019 | Science Bulletin2019，64，15： | 2 |
| 13 | The MING proposal at SHINE:megahertz cavity enhanced X‑ray generation显示文摘The cavity-based X-ray free-electron laser(XFEL)has promise in producing fully coherent pulses with a bandwidth of a few meV and very stable intensity,whereas the currently existing self-amplified spontaneous emission(SASE)XFEL is capable of generating ultra-short pulses with chaotic spectra.In general,a cavity-based XFEL can provide a spectral brightness three orders of magnitude higher than that of the SASE mode,thereby opening a new door for cutting-edge scientific research.With the development of superconducting MHz repetition-rate XFEL facilities such as FLASH,European-XFEL,LCLS-II,and SHINE,practical cavity-based XFEL operations are becoming increasingly achievable.In this study,megahertz cavity enhanced X-ray generation(MING)is proposed based on China’s first hard XFEL facility-SHINE,which we refer to as MING@SHINE. | Nan‑Shun Huang Zi‑Peng Liu Bang‑Jie Deng Zi‑Han Zhu Shao‑Hua Li Tao Liu Zheng Qi Jia‑Wei Yan Wei Zhang Sheng‑Wang Xiang Yang‑Yang Lei Ya Zhu Yong‑Zhou He Qi‑Bing Yuan Fei Gao Rong‑Bing Deng Sen Sun Zhi‑Di Lei Zhi‑Qiang Jiang Meng‑Qi Duan Yuan Zhuan Xue‑Fang Huang Peng‑Cheng Dong Zhong‑Liang Li Shang‑Yu Si Lian Xue Si Chen Yong‑Fang Liu Ya‑Jun Tong Hai‑Xiao Deng Zhen‑Tang Zhao | 2023 | Nuclear Science and Techniques2023，34，1： | 2 |
| 14 | Density-functional-theory predictions of mechanical behaviour and thermal properties as well as experimental hardness of the Ga-bilayer Mo_(2)Ga_(2)C显示文摘Mo_(2)Ga_(2)C is a new MAX phase with a stacking Ga-bilayer as well as possible unusual properties.To understand this unique MAX phase structure and promote possible future applications,the structure,chemical bonding,and mechanical and thermodynamic properties of Mo_(2)Ga_(2)C were investigated by first-principles.Using the“bond stiffness”model,the strongest covalent bonding(1162 GPa)was formed between Mo and C atoms in Mo_(2)Ga_(2)C,while the weakest Ga–Ga(389 GPa)bonding was formed between two Ga-atomic layers,different from other typical MAX phases.The ratio of the bond stiffness of the weakest bond to the strongest bond(0.33)was lower than 1/2,indicating the high damage tolerance and fracture toughness of Mo_(2)Ga_(2)C,which was confirmed by indentation without any cracks.The high-temperature heat capacity and thermal expansion of Mo_(2)Ga_(2)C were calculated in the framework of quasi-harmonic approximation from 0 to 1300 K.Because of the metal-like electronic structure,the electronic excitation contribution became more significant with increasing temperature above 300 K. | Xinxin QI Weilong YIN Sen JIN Aiguo ZHOU Xiaodong HE Guangping SONG Yongting ZHENG Yuelei BAI | 2022 | Journal of Advanced Ceramics2022，11，2： | 2 |
| 15 | High-throughput 16S rDNA sequencing of the pulmonary microbiome of rats with allergic asthma显示文摘A decrease in microbial infection in adolescents is implicated with an increase in the incidence of asthma and allergic diseases in adulthood,indicating that the microbiome plays a critical role in asthma.However,the microbial composition of the lower respiratory tract remains unclear,hindering the further exploration of the pathogenesis of asthma.This study aims to explore the microbial distribution and composition in the lungs of normal rats and rats with allergic asthma via 16S rDNA sequencing.The DNA of the pulmonary microbiome was extracted from the left lungs collected from normal control group(NC),saline control group(SC),and allergic asthma group(AA)under aseptic conditions.After the 16s rDNA V4eV5 region was amplified,the products were sequenced using Illumina high-throughput technology and subjected to operational taxonomic unit(OTU)cluster and taxonomy analysis.The OTU values of AA increased significantly compared with those of NC and SC.Microbiome structure analysis showed that the dominant phylum of the pulmonary microbiome changed from Proteobacteria in NC to Firmicutes in AA.Linear discriminant analysis indicated that the key microbiomes involved in the three groups varied. | Yang Xiong Sen Hu Hongyao Zhou Hui Zeng Xuan He Dongni Huang Xiaoyu Li | 2020 | Genes & Diseases2020，7，2： | 2 |
| 16 | Genome-wide study reveals an important role of spontaneous autoimmunity, cardiomyocyte differentiation defect and antiangiogenic activities in gender-specific gene expression in Keshan disease显示文摘 | He Shulan Tan Wuhong Wang Sen Wu Cuiyan Wang Pan Wang Bin Su Xiaohui Zhao Junjie Guo Xiong Xiang Youzhang | 2014 | Chinese Medical Journal2014，，1： | 2 |
| 17 | An Investigation on the Molecular Characteristics and Intracellular Growth Ability among Environmental and Clinical Isolates of Legionella pneumophila in Sichuan Province, China显示文摘Objective To investigate the molecular characteristics and intracellular growth ability of Legionella pneumophila(L. pneumophila) strains from 1989 to 2016 in Sichuan Province, China. Methods Seventy-nine isolates of L. pneumophila were collected from environmental and clinical sources, including cooling towers, hot springs, bath water, fountains, and patients, and identified with 16 S rRNA gene analysis and serum agglutination assay. The isolates were then typed by Sequence-Based Typing(SBT), and Genotyping of forty-two LP1 strains were analyzed by means of multiple-locus VNTR analysis with 8 loci(MLVA-8). All strains were further analyzed for two virulence genes: Legionella vir homologue(lvh) and repeats in structural toxin(rtx A). The intracellular growth ability of 33 selected isolates was determined by examining their interaction with J774 cells. Results All isolates were identified to L. pneumophila including 11 serogroups, among which the main serogroup were LP1, accounting for 54.43%. Thirty-three different sequence types(STs) from five main clonal groups and five singletons were identified, along with 8 different MLVA patterns. Both the lvh and rtx A loci were found in all 79 strains. Thirty isolates showed high intracellular growth ability in J774 cells. Conclusion L. pneumophila is a potential threat to public health, and effective control and prevention strategies are urgently needed. | ZENG Lin Zi LIAO Hong Yu LUO Long Ze HE Shu Sen QIN Tian ZHOU Hai Jian LI Hong Xia CHEN Da Li CHEN Jian Ping | 2019 | Biomedical and Environmental Sciences2019，32，7： | 1 |
| 18 | Clinical characteristics of pulmonary hypertension in bronchiectasis显示文摘作为 bronchiectasis 的复杂并发症,肺的高血压(PH ) 与增加的死亡被联系。然而,血液动力学的特征和肺的动脉的高血压的功效(哼) 在有 bronchiectasis 和 PH 的病人的治疗仍然保持未知。有双边的 bronchiectasis 和并发的 PH 的病人在学习被包括。在基线并且在后续期间的耐心的特征,以及幸存,被分析。这观察研究与 51.5 年的吝啬的年龄在 36 个病人被进行(范围, 1774 年) 。走距离的 6 min 是 300.8 搠睯 吗?? | Lan Wang Sen Jiang Jingyun Shi Sugang Gong Qinhua Zhao Rong Jiang Ping Yuan Bigyan Pudasaini Jing He Zhicheng Jing Jinming Liu | 2016 | Frontiers of Medicine2016，10，3： | 1 |
| 19 | Contribution of MDR1 gene polymorphisms on IBD predisposition and response to glucocorticoids in IBD in a C hinese population显示文摘 | Qing Fan Yang Bai Li Chen Qing Sen Zhang Zhen Hua Zhu Bin Hu Yao He Xiang Gao Yi Ming Wang Pin Jin Hu Min Hu Chen Zhi Rong Zeng | 2015 | Journal of Digestive Diseases2015，，1： | 1 |
| 20 | Intestinal microbiota and juvenile idiopathic arthritis:current understanding and future prospective显示文摘Background Juvenile idiopathic arthritis(JIA)characterized by arthritis of unknown origin is the most common childhood chronic rheumatic disease,caused by both host genetic factors and environmental triggers.Recent evidence has mounted to focus on the intestinal microbiota,a potentially recognized set of environmental triggers affecting JIA development.Here we offer an overview of recently published animal and human studies that support the impact of intestinal microbiota in JIA.Data sources We searched PubMed for animal and human studies publications with the search terms'intestinal microbiota or gut microbiota'and'juvenile idiopathic arthritis or juvenile chronic arthritis or juvenile rheumatoid arthritis or childhood rheumatoid arthritis or pediatric rheumatoid arthritis'.Results Several comparative studies have demonstrated that intestinal microbial alterations might be triggers in disease pathogenesis.Alternatively,a slice of studies has suggested environmental triggers in early life might disrupt intestinal microbial colonization,including cesarean section,formula feeding,and antibiotic exposure.Aberrant intestinal microbiota may influence the development of JIA by mediating host immune programming and by altering mucosal permeability.Conclusions Specific microbial factors may contribute to the pathogenesis of JIA.Intensive studies,however,are warranted to investigate the causality between intestinal dysbiosis and JIA and the mechanisms behind these epidemiologic relationships.Studies are also needed to design the best interventional administrations to restore balanced intestinal microbial communities. | Le Xin Feng He Sen Li Zhi-Xuan Zhou Xiao-Lin Ma | 2021 | World Journal of Pediatrics2021，17，1： | 1 |
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