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| 1 | Hepatitis B and C virus-induced hepatitis: Apoptosis, autophagy, and unfolded protein response显示文摘AIM: To investigate the co-incidence of apoptosis, autophagy, and unfolded protein response(UPR) in hepatitis B(HBV) and C(HCV) infected hepatocytes.METHODS: We performed immunofluorescence confocal microscopy on 10 liver biopsies from HBV and HCV patients and tissue microarrays of HBV positive liver samples. We used specific antibodies for LC3β, cleaved caspase-3, BIP(GRP78), and XBP1 to detect autophagy, apoptosis and UPR, respectively. AntiHCV NS3 and anti-HBs antibodies were also used to confirm infection. We performed triple blind counting of events to determine the co-incidence of autophagy(LC3β punctuate), apoptosis(cleaved caspase-3), and unfolded protein response(GRP78) with HBV and HCV infection in hepatocytes. All statistical analyses were performed using SPSS software for Windows(Version 16 SPSS Inc, Chicago, IL, United States). P-values < 0.05 were considered statistically significant. Statistical analyses were performed with Mann-Whitney test to compare incidence rates for autophagy, apoptosis, and UPR in HBV- and HCV-infected cells and adjacent noninfected cells.RESULTS: Our results showed that infection of hepatocytes with either HBV and HCV induces significant increase(P < 0.001) in apoptosis(cleavage of caspase-3), autophagy(LC3β punctate), and UPR(increase in GRP78 expression) in the HCV- and HBVinfected cells, as compared to non-infected cells of the same biopsy sections. Our tissue microarray immunohistochemical expression analysis of LC3β in HBV^(Neg) and HBV^(Pos) revealed that majority of HBVinfected hepatocytes display strong positive stainingfor LC3β. Interestingly, although XBP splicing in HBVinfected cells was significantly higher(P < 0.05), our analyses show a slight increase of XBP splicing was in HCV-infected cells(P > 0.05). Furthermore, our evaluation of patients with HBV and HCV infection based on stage and grade of the liver diseases revealed no correlation between these pathological findings and induction of apoptosis, autophagy, and UPR.CONCLUSION: The results of this study indicate that HCV and HBV infection activates apoptosis, autophagy and UPR, but slightly differently by each virus. Further studies are warranted to elucidate the interconnections between these pathways in relation to pathology of HCV and HBV in the liver tissue. | Behzad Yeganeh Adel Rezaei Moghadam Javad Alizadeh Emilia Wiechec Seyed Moayed Alavian Mohammad Hashemi Bita Geramizadeh Afshin Samali Kamran Bagheri Lankarani Martin Post Payam Peymani Kevin M Coombs Saeid Ghavami | 2015 | World Journal of Gastroenterology2015，21，47： | 13 |
| 2 | Hepatocellular carcinoma in Egypt: A single center study over a decade显示文摘AIM: To identify the trend, possible risk factors and any pattern change of hepatocellular carcinoma (HCC) in Egyptover a decade.METHODS: All HCC patients attending Cairo Liver Center between January 1993 and December 2002, were enrolledin the study. Diagnosis of HCC was based on histopath ological examination and/or detection of hepatic focal lesions by two imaging techniques plus α-fetoprotein level above 200 ng/mL. The duration of the study was divided into two periods of 5 years each; period Ⅰ (1993-1997) and period Ⅱ (1998-2002). Trend, demographic features of patients (age, gender, and residence), risk factors (HBsAg,HCV-Ab, schistosomiasis and others) and pattern of the focal lesions were compared between the two periods.Logistic regression model was fitted to calculate the adjusted odds ratios for the potential risk factors. The population attributable risk percentage was calculated to estimate the proportion of HCC attributed to hepatitis B and C viral infections.RESULTS: Over a decade, 1 328 HCC patients out of 22 450 chronic liver disease (CLD) patients were diagnosed with an overall proportion of 5.9%. The annual proportion of HCC showed a significant rising trend from 4.0% in 1993 to 7.2% in 2002 (P = 0.000). A significant increase in male proportion from 82.5% to 87.6% (P = 0.009); M/F from 5:1 to 7:1 and a slight increase of the predominant age group (40-59 years) from 62.6% to 66.8% (P = 0.387)in periods Ⅰ and Ⅱ respectively, reflecting a shift to younger age group. In the bivariate analysis, HCC was significantly higher in rural residents, patients with history of schistosomiasis and/or blood transfusion. Yet, after adjustment, these variables did not have a significant risk for development of HCC. There was a significant decline of HBsAg from 38.6% to 20.5% (P = 0.000), and a slight increase of HCV-Ab from 85.6% to 87.9% in periods Ⅰ and Ⅱ respectively. HBV conferred a higher risk to develop HCC more than HCV in period Ⅰ (OR 1.9 vs 1.6) and period Ⅱ (OR 2.7 vs 2.0), but the relative contribution of HBV for development of HCC declined in period Ⅱ compared to period Ⅰ (PAR% 4.2%, 21.32%). At presentation,diagnostic α-fetoprotein level (≥200 ng/mL) was demonstrated in 15.6% vs28.9% and small HCC (≤3 cm)represented 14.9% vs 22.7% (P = 0.0002) in periods Ⅰ and Ⅱ respectively.CONCLUSION: Over a decade, there was nearly a twofold increase of the proportion of HCC among CLD patients in Egypt with a significant decline of HBV and slight increase of HCV as risk factors. α-Fetoprotein played a limited role in diagnosis of HCC, compared to imaging techniques.Increased detection of small lesions at presentation reflects increased awareness of the condition. | Adbel-Rahman El-Zayadi Hanaa M Badran Eman MF Barakat Mohy El-Deen Attia Sherine Shawky Mostafa K Mohamed Osaima Selim Ahmed Saeid | 2005 | World Journal of Gastroenterology2005，11，33： | 5 |
| 3 | The use of bentonite for removal of aromatic organics from contaminated soil 显示文摘 | Saeid G Bowers M T Bodocsi A | 1997 | Journal of Colloid and Interface Science1997，196，： | 1 |
| 4 | Reflectance Vis/NIR spectroscopy for nondestructive taste characterization of Valencia oranges显示文摘 | Bahareh J S Saeid M Ezzedin Mohajeranib | 2012 | Computers and Electronics in Agriculture2012，85，： | 1 |
| 5 | Two-mode over- constrained three-DOFs rotational-translational linear-motor-based parallel-kinematics mechanism for machine tool applications 显示文摘 | Sameh R HervJacques M Saeid N Hieul T | 2007 | Robotiea2007，25，4： | 1 |
| 6 | Multiple inflammatory prognostic factors in acute coronary syndromes: a prospective inception cohort study 显示文摘 | Hatmi Z N Saeid A K Broumand M A | 2010 | Acta Med lran2010，48，1： | 1 |
| 7 | Radiation processingtechniques in remediation of pollutants,and the role of the IAEAin supporting capacity building in developing countries 显示文摘 | Mohammad S Saeid H Sampa M H | 2012 | Radiation Physics and Chemistry2012，81，： | 1 |
| 8 | Bipolar Fuzzy K-algebras显示文摘 | Akram M Saeid A B Shum K P | | 0，，： | 1 |
| 9 | Urinary excretion of n-acetyl-beta-D-glucosaminidase and retinol binding protein as alternative indicators of nephropathy in patients with type 1 diabetes mellitus 显示文摘 | Salem M A el-Habashy S A Saeid O M | 2002 | Pediatr Diabetes2002，3，1： | 1 |
| 10 | Shear analysis and design of ductile steel plate walls显示文摘 | SAEID S G CARIOS E VENTURA M A | 2005 | Journal of Structural Engineering2005，131，6： | 1 |
| 11 | 查看详情显示文摘 | Mehdi S K Vahid H A Farid B S Saeid R R Hossein R M | | 0，，02： | 1 |
| 12 | Some types of filters inBL-algebras显示文摘 | Haveshki M Saeid A B Eslami E | 2006 | Soft Computing2006，10，2： | 1 |
| 13 | Some types of filters in BL-algebras显示文摘 | Haveshki M Saeid A B Eslami E | 2006 | Soft Comput2006，10，： | 1 |
| 14 | Some types of filters in BL-algebras显示文摘 | HAVESHKI M BORUMAND SAEID A ESLAMI E | 2006 | Soft Computing2006，10，8： | 1 |
| 15 | Radiation treat- ment for sterilization of packaging materials显示文摘 | Saeid M H O Sampa M H Chmielewski G A | 2007 | Radiation Phys ics and Chemistry2007，76，8： | 1 |
| 16 | Seasonal changes of fatty acid ompositions in overwintering larvae of rice stem borer, Chilo suppressalis (Lepidoptera: Pyralidae) 显示文摘 | Maryam A Saeid M Mohsen B | 2007 | Journal of Asia - Pacific Entomology2007，10，1： | 1 |
| 17 | A unified approach to form error evaluation显示文摘 | TIMOTHY W SAEID M BEHROOZ F | 2002 | Precision Engineering2002，26，： | 1 |
| 18 | Embryological basis of some aspects of cerebral vascular fistulas and malformations显示文摘 | Sean M Saeid M Douglas LJ | 1996 | J Neurosurg1996，85，： | 1 |
| 19 | Automatic segmentation of digitized data for reverse engineering applications显示文摘 | Abdalla A Saeid M Behrooz F | 2000 | IIE Transactions2000，32，1： | 1 |
| 20 | The level and composition of tax revenue in developing countries: Evidence from unbalanced panel data显示文摘 | Saeid M | 2008 | International Review of Economics and Fi- nance2008，17，： | 1 |
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