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| 1 | Genetic Structure and Diversity of Parental Cultivars Involved in China Mainland Sugarcane Breeding Programs as Inferred from DNA Microsatellites显示文摘To understand genetic structure and diversity of parental cultivars involved in China Mainland sugarcane breeding programs,92 elite parents and 4 wild relatives were genotyped with 18 microsatellite DNA markers.The genetic similarity(GS) values among the cultivars ranged from 0.346 to 0.960 with an average of 0.533.Among the introduced cultivars,India accessions had the closest genetic distance to China Mainland accessions(0.447),while Australia accessions have the furthest distance(0.503).A comparison of allelic diversity among geographical origins showed that there were 22 China Mainland specific alleles,of which 28% were derived from native S.spontaneaum germplasm in China.Model-based genetic structure,clustering,and principal components analyses consistently revealed there were five groups within the 96 accessions.Groups 1,2,4,and 5 consisted of all cultivars and group 3 only contained wild germplasm.Group 2 was characterized as the Introduction group with 46 cultivars predominantly introduced from Australia,Taiwan of China,India,and USA.Groups 1,4,and 5 consisted of cultivars mostly originated from China Mainland,defined as the Complex group,Yacheng lines group,and F134/CP72-1210 group,respectively,upon their pedigree.By understanding the genetic relationships among the parental cultivars,breeders can gain a rational basis for expanding the gene pool and select the best parental accessions for crossing. | QI Yong-wen PAN Yong-bao LAO Fang-ye ZHANG Chui-ming FAN Li-na HE Hui-yi LIU Ruin WANG Qin-nan LIU Shao-mou LIU Fu-ye LI Qi-wei DENG Hai-hua | 2012 | Journal of Integrative Agriculture2012，11，11： | 6 |
| 2 | Characterization of polyclonal antibodies against nonstructural protein 9 from the porcine reproductive and respiratory syndrome virus显示文摘Porcine reproductive and respiratory syndrome(PRRS) is considered to be one of the most important infectious diseases impacting the swine industry and is characterized by reproductive failure in late term gestation in sows and respiratory disease in pigs of all ages.The nonstructural protein 9 gene,Nsp9,encoding the RNA-dependent RNA polymerase,is generally regarded as fairly conserved when compared to other viral proteins.Antibodies against Nsp9 will be of great importance for the diagnosis and treatment of the causal agent,PRRS virus.A study was undertaken to generate polyclonal antibodies against the immunodominant Nsp9.For this purpose,the Nsp9 was expressed in Escherichia coli and subsequently used as an antigen to immunize New Zealand rabbits.Antiserum was identified via an indirect ELISA,and then verified based on the ability to react with both naturally and artificially expressed Nsp9.Results of virus neutralization test showed that this antiserum could not neutralize the PRRSV.Nevertheless,this antiserum as a diagnostic core reagent should prove invaluable for further investigations into the mechanism of PRRS pathogenesis. | Mengmeng ZHAO Juanjuan QIAN Jiexiong XIE Tiantian CUI Songling FENG Guoqiang WANG Ruining WANG Guihong ZHANG | 2016 | Frontiers of Agricultural Science and Engineering2016，3，2： | 2 |
| 3 | Extremely low penetrance of deafness associated with the mitochondrial 12S rRNA mutation in 16 Chinese families: Implication for early detection and prevention of deafness显示文摘 | Pu Dai Xin Liu Dongyi Han Yaping Qian Deliang Huang Huijun Yuan Weiming Li Fei Yu Ruining Zhang Hongyan Lin Yong He Youjun Yu Quanzhu Sun Huaiyi Qin Ronghua Li Xin Zhang Dongyang Kang Juyang Cao Wie-Yen Young Min-Xin Guan | 2005 | Biochemical and Biophysical Research Communications2005，，1： | 2 |
| 4 | Doppler ultrasanie color flow inaging in the study of breast cancer:Preliminathy findings显示文摘 | Adler D D Carsou P L Ruin J M | 1999 | Ultrasound Med Bio11999，16，3： | 1 |
| 5 | Study ongrid adaptability testing methodology for wind tuibines显示文摘 | Li Shaolin Qin Shiyao Wang Ruining | 2013 | Journal of Modern Power System and Clean Energy2013，1，1： | 1 |
| 6 | The value of serum CA125 and association CA125/ CA199 in endometrial carcinoma显示文摘 | Cherch OL Dosslole S Ruin G A | 1999 | Eur J Gynaecol Oncol1999，20，4： | 1 |
| 7 | The value of serum CA125 and association CA125 CA199 in endometrial 显示文摘 | Cherchi OL Dossole S Ruin GA | 1999 | Eur J Gynaecol Oncol1999，20，4： | 1 |
| 8 | A novel blue light generation by frequency doubling of a diode-pumped Nd:YAG thin disk laser显示文摘 | Lin Xuechun Li Ruining | 2004 | Optics Communications2004，237，： | 1 |
| 9 | Effects of La3+ on growth, transformation, and gene expression of Escherichia coli 显示文摘 | Wenhua L Ruining Z Zhixiong X | 2003 | Biol Trace Elem Res2003，94，2： | 1 |
| 10 | Constituents of the flowers of Punica granatum显示文摘 | Rufeng Wang Wei Wang Liang Wang Ruining Liu Yi Ding Lijun Du | 2006 | Fitoterapia2006，，7： | 1 |
| 11 | Rare Metal Materials and Engineering显示文摘 | Wang Ruining Xi Zhengping Zhao Yongqing | 2008 | 37(8):13562008，37，8： | 1 |
| 12 | Analysis of DNA methylation in Arabi- dopsis thaliana based on methylation-sensitive AFLP markers显示文摘 | CERVARA M T RUIN -GARCIA L MARTINEZ- ZAPATER J M | 2002 | Mol Genet Genomics2002，268，4： | 1 |
| 13 | Doppleruhrasoundeolor flow imaging in the study of breast cancer; Preliminary findings显示文摘 | Adler DD Carson PL Ruin JM | 1990 | Ultrasound Med Biol1990，16，： | 1 |
| 14 | Doppler ultrasound color flow imaging in the study of breast cancer:preliminary findings显示文摘 | Adler DD Carson PL Ruin JM | 2009 | Ultrasound Med Biol2009，1616，3： | 1 |
| 15 | Uniformly-sized, molecularly imprinted polymers for nicotine by precipitation polymerization 显示文摘 | Haruyo S Kaori H Ruin M | 2006 | J Chromatogr A2006，1134，12： | 1 |
| 16 | The value of serum CA125 and association CA125/CA199 in endometrial carcinoma显示文摘 | Cherch OL Dosslole S Ruin GA | 1999 | Eur J Gynaecol Oncol1999，20，4： | 1 |
| 17 | Intestinal varices:treatment with the transjugular intrahepatic portosystemic shunt 显示文摘 | Haskal ZJ Scott M Ruin RA | 1994 | Radiology1994，191，1： | 1 |
| 18 | The value of serum CA125 and association CA125 CA199 in endometrial 显示文摘 | Cherchi OL Dossole S Ruin GA | 1999 | Eur J Gynaccol Oncol1999，20，4： | 1 |
| 19 | Electrostatic Actuating Bendable Flat Electrode for Micro Electrochemical Machining显示文摘In micro-electrochemical machining(μECM), material dissolution takes place at very close vicinity of tool electrode due to localization of electric field. Controlling the gap between tool electrode and workpiece is the key to μECM. Therefore, a new method is proposed to solve a variety of problems in small gap control. In the present context, experiments were carried out with an indigenously developed setup to fabricate cylindrical arrays. During the machining process, the flat electrode bends due to electrostatic force in pulse on-time, which self-adaptively narrows the gap between the electrode and the workpiece. The workpiece material will be removed once the gap meets the processing condition. Therefore, this method has advantages of reducing dependence on high precision machine tools and of avoiding complex servo control. The flat electrode quickly restores to its original condition when it is in pulse off-time, making the gap much larger than that in traditional electrochemical machining(ECM). The large gap benefits debris removing, which improves the machining accuracy. The influence of different experimental parameters on accuracy and efficiency during the machining process has been investigated. It is observed that with the increase in applied voltage or concentration of electrolyte, the material removal rate and the process gap both increase. The detailed analysis of the experimental results is described in this paper. | Ruining Huang Xiaokun Zhu | 2018 | Nanotechnology and Precision Engineering2018，1，2： | 1 |
| 20 | Development of an en- hanced efficient secured multi-hop routing technique for wireless sensor networks 显示文摘 | RUIN I K SAKTHIVEL N K Subasree S | 2013 | International Journal of Innovative Research in Computer and Communication Engineering2013，1，3： | 1 |
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