|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Genetic Structure and Diversity of Parental Cultivars Involved in China Mainland Sugarcane Breeding Programs as Inferred from DNA Microsatellites显示文摘To understand genetic structure and diversity of parental cultivars involved in China Mainland sugarcane breeding programs,92 elite parents and 4 wild relatives were genotyped with 18 microsatellite DNA markers.The genetic similarity(GS) values among the cultivars ranged from 0.346 to 0.960 with an average of 0.533.Among the introduced cultivars,India accessions had the closest genetic distance to China Mainland accessions(0.447),while Australia accessions have the furthest distance(0.503).A comparison of allelic diversity among geographical origins showed that there were 22 China Mainland specific alleles,of which 28% were derived from native S.spontaneaum germplasm in China.Model-based genetic structure,clustering,and principal components analyses consistently revealed there were five groups within the 96 accessions.Groups 1,2,4,and 5 consisted of all cultivars and group 3 only contained wild germplasm.Group 2 was characterized as the Introduction group with 46 cultivars predominantly introduced from Australia,Taiwan of China,India,and USA.Groups 1,4,and 5 consisted of cultivars mostly originated from China Mainland,defined as the Complex group,Yacheng lines group,and F134/CP72-1210 group,respectively,upon their pedigree.By understanding the genetic relationships among the parental cultivars,breeders can gain a rational basis for expanding the gene pool and select the best parental accessions for crossing. | QI Yong-wen PAN Yong-bao LAO Fang-ye ZHANG Chui-ming FAN Li-na HE Hui-yi LIU Ruin WANG Qin-nan LIU Shao-mou LIU Fu-ye LI Qi-wei DENG Hai-hua | 2012 | Journal of Integrative Agriculture2012，11，11： | 6 |
| 2 | Characterization of polyclonal antibodies against nonstructural protein 9 from the porcine reproductive and respiratory syndrome virus显示文摘Porcine reproductive and respiratory syndrome(PRRS) is considered to be one of the most important infectious diseases impacting the swine industry and is characterized by reproductive failure in late term gestation in sows and respiratory disease in pigs of all ages.The nonstructural protein 9 gene,Nsp9,encoding the RNA-dependent RNA polymerase,is generally regarded as fairly conserved when compared to other viral proteins.Antibodies against Nsp9 will be of great importance for the diagnosis and treatment of the causal agent,PRRS virus.A study was undertaken to generate polyclonal antibodies against the immunodominant Nsp9.For this purpose,the Nsp9 was expressed in Escherichia coli and subsequently used as an antigen to immunize New Zealand rabbits.Antiserum was identified via an indirect ELISA,and then verified based on the ability to react with both naturally and artificially expressed Nsp9.Results of virus neutralization test showed that this antiserum could not neutralize the PRRSV.Nevertheless,this antiserum as a diagnostic core reagent should prove invaluable for further investigations into the mechanism of PRRS pathogenesis. | Mengmeng ZHAO Juanjuan QIAN Jiexiong XIE Tiantian CUI Songling FENG Guoqiang WANG Ruining WANG Guihong ZHANG | 2016 | Frontiers of Agricultural Science and Engineering2016，3，2： | 2 |
| 3 | Study ongrid adaptability testing methodology for wind tuibines显示文摘 | Li Shaolin Qin Shiyao Wang Ruining | 2013 | Journal of Modern Power System and Clean Energy2013，1，1： | 1 |
| 4 | Constituents of the flowers of Punica granatum显示文摘 | Rufeng Wang Wei Wang Liang Wang Ruining Liu Yi Ding Lijun Du | 2006 | Fitoterapia2006，，7： | 1 |
| 5 | Rare Metal Materials and Engineering显示文摘 | Wang Ruining Xi Zhengping Zhao Yongqing | 2008 | 37(8):13562008，37，8： | 1 |
| 6 | Efficient purification of cell culture-derived classical swine fever virus by ultrafiltration and size-exclusion chromatography显示文摘Large-scale production of cell culture-based classical swine fever virus(CSFV)vaccine is hampered by the adverse reactions caused by contaminants from host cell and culture medium.Hence,we have developed an efficient method for purifying CSFV from cell-culture medium.Pure viral particles were obtained with two steps of tangential-flow filtration(TFF)and size-exclusion chromatography(SEC),and were compared with particles from ultracentrifugation by transmission electron microscopy(TEM),infectivity and recovery test,and real time fluorescent quantitative PCR(FQ-PCR).TFF concentrated the virus particles effectively with a retention rate of 98.5%,and 86.2%of viral particles were obtained from the ultrafiltration retentate through a Sepharose 4 F F column on a biological liquid chromatography system.CSFV purified by TFF-SEC or ultracentrifugation were both biologically active from 1.0×10–4.25 TCID50·mL^(–1) to 3.0×10^(–6.25) TCID50·mL^(–1),but the combination of TFF and SEC produced more pure virus particles than by ultracentrifugation alone.In addition,pure CSFV particles with the expected diameter of 40–60 nm were roughly spherical without any visible contamination.Mice immunized with CSFV purified by TFF-SEC produced higher antibody levels compared with immunization with ultracentrifugation-purified CSFV(P<0.05).The purification procedures in this study are reliable technically and feasible for purification of large volumes of viruses. | Ruining WANG Yubao ZHI Junqing GUO Qingmei LI Li WANG Jifei YANG Qianyue JIN Yinbiao WANG Yanyan YANG Guangxu XING Songlin QIAO Mengmeng ZHAO Ruiguang DENG Gaiping ZHANG | 2015 | Frontiers of Agricultural Science and Engineering2015，2，3： | 1 |
| 7 | Epithelial cells mimic immune cells: a novel path toward tumor immunotherapy显示文摘Recently,we have shown that FOXP3,a critical transcription factor in regulatory T cells(Tregs),is expressed in pancreatic epithelial cells,and restrain the activity of CD8+T cells by upregulating PD-L1,which in turn regulates immune escape in pancreatic ductal adenocarcinoma(PDAC)^(1).On the basis of a series of studies of our laboratory,we hypothesize that a subset of pancreatic epithelial cells mimics the phenotype and function of Tregs(named quasi-Tregs or qTregs);this concept has been supported by a peer-reviewed commentary^(2).Moreover,evidence suggests that tumor epithelial cells can mimic other types of immune cells and participate in the formation of a tumor immunosuppressive microenvironment(TIM). | Ruining Gong Yan Huang Xiaoxuan Wang Xiaobing Chen Zibin Tian He Ren | 2021 | Cancer Biology & Medicine2021，18，4： | 0 |
| 8 | CW Self-Frequency-Doubling Laser Operating on Nd:MgO:LiNbO_(3) High-Gain Polarization显示文摘The CW second-harmonic wave of high-gain polarization atλ=l.085μm was demonstrated with Nd:MgO:LiNbO_(3) self-frequency-doubling laser cavity inserted with a quarter wave-plate of the fundamental wavelength.The pump power threshold for the second-harmonic generation as low as 9.9mW was achieved.The maximum second-harmonic output was 11.4 m W,the conversion efficiency for the total second-harmonic output was 11.3%per Watt. | LI Ruining WANG Junmin LIANG Xiaoyan XIE Changde PENG Kunchi XU Guanfeng | 1993 | Chinese Physics Letters1993，10，4： | 0 |
| 9 | Interactive Effect of Grain Orientation and Grain Size on Magnetic Properties of Fe-78 wt% Ni Ribbons显示文摘Fe-78 wt% Ni ribbons were prepared by the melt spinning technique and the interactive contribution of the grain size and grain orientation on the magnetic properties was examined. Heat treatment at 673 K for 1 h followed by furnace cooling was performed to show the annealing impact. At three wheel speeds of 10, 20, and 30 m/s, the saturation magnetization nearly does not change. High wheel speed and heat treatment are inclined to promote the growth of <001> grains. Although the <001> orientation is not the easy axis of magnetization, the improvement of the texture in this direction makes the coercivity decrease, which counteracts the inverse effect of the grain size at high wheel speed. It indicates that for preparing soft magnetic ribbons, the interactive contribution of grain orientation variation and the grain size should be considered. | Nan Wang Ruining Yang Wenjing Yao Jinfeng Xu Xixing Wen | 2012 | Journal of Materials Science & Technology2012，28，9： | 0 |
| 10 | Clinical features of 162 fatal cases of COVID-19:a multi-center retrospective study显示文摘Background:The coronavirus disease 2019(COVID-19)has affected approximately 2 million individuals worldwide;however,data regarding fatal cases have been limited.Objective:To report the clinical features of 162 fatal cases of COVID-19 from 5 hospitals in Wuhan between December 30,2019 and March 12,2020.Methods:The demographic data,signs and symptoms,clinical course,comorbidities,laboratory findings,computed tomographic(CT)scans,treatments,and complications of the patients with fatal cases were retrieved from electronic medical records.Results:The median patient age was 69.5(interquartile range:63.0–77.25)years,and 80%of the patients were over 61 years.A total of 112(69.1%)patients were men.Hypertension(45.1%)was the most common comorbidity,while 59(36.4%)patients had no comorbidity.At admission,131(81.9%)patients had severe or critical COVID-19,whereas 39(18.1%)patients with hypertension or chronic lung disease had moderate COVID-19.In total,126(77.8%)patients received antiviral treatment,while 132(81.5%)patients received glucocorticoid treatment.A total of 116(71.6%)patients were admitted to the intensive care unit(ICU),and 137(85.1%)patients received mechanical ventilation.Most patients received mechanical ventilation before ICU admission.Approximately 93.2%of the patients developed respiratory failure or acute respiratory distress syndrome.There were no significant differences in the inhospital survival time among the hospitals(P=0.14).Conclusion:Young patients with moderate COVID-19 without comorbidity at admission could also develop fatal outcomes.The in-hospital survival time of the fatal cases was similar among the hospitals of different levels in Wuhan. | Xianlong Zhou Guoyong Ding Qing Fang Jun Guo Luyu Yang Ping Wang Shou-Zhi Fu Ang Li Jian Xia Jiangtao Yu Jianyou Xia Min Ma Zhuanzhuan Hu Lei Huang Ruining Liu Cheng Jiang Shaoping Li Mingxia Yu Xizhu Xu Yan Zhao Quan Hu Weijia Xing Zhigang Zhao | 2022 | Emergency and Critical Care Medicine2022，2，3： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员