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| 1 | High-quality genome assembly of 'Cuiguan' pear (Pyrus pyrifolia) as a reference genome for identifying regulatory genes and epigenetic modifications responsible for bud dormancy显示文摘Dormancy-associated MADS-box(DAM)genes serve as crucial regulators of the endodormancy cycle in rosaceous plants.Although pear DAM genes have been identified previously,the lack of a high-quality reference genome and techniques to study gene function have prevented accurate genome-wide analysis and functional verification of such genes.Additionally,the contribution of other genes to the regulation of endodormancy release remains poorly understood.In this study,a high-quality genome assembly for'Cuiguan'pear(Pyrus pyrifolia),which is a leading cultivar with a low chilling requirement cultivated in China,was constructed using PacBio and Hi-C technologies.Using this genome sequence,we revealed that pear DAM genes were tandemly clustered on Chr8 and Chr15 and were differentially expressed in the buds between'Cuiguan'and the high-chilling-requirement cultivar'Suli'during the dormancy cycle.Using a virus-induced gene silencing system,we determined the repressive effects of DAM genes on bud break.Several novel genes potentially involved in the regulation of endodormancy release were identified by RNA sequencing and H3K4me3 chromatin immunoprecipitation sequencing analyses of‘Suli'buds during artificial chilling using the new reference genome.Our findings enrich the knowledge of the regulatory mechanism underlying endodormancy release and chilling requirements and provide a foundation for the practical regulation of dormancy release in fruit trees as an adaptation to climate change. | Yuhao Gao Qinsong Yang Xinhui Yan Xinyue Wu Feng Yang Jianzhao Li Jia Wei Junbei Ni Mudassar Ahmad Songling Bai Yuanwen Teng | 2021 | Horticulture Research2021，8，1： | 5 |
| 2 | From octahedral structure motif to sub-nanosecond phase transitions in phase change materials for data storage显示文摘Phase change random access memory(PCRAM) has been successfully applied in the computer storage architecture, as storage class memory, to bridge the performance gap between DRAM and Flashbased solid-state drive due to its good scalability, 3 D-integration ability, fast operation speed and compatible with CMOS technology. Focusing on phase change materials and PCRAM for decades, we have successfully developed 128 Mb embedded PCRAM chips, which can meet the requirements of most embedded systems.3 D Xpoint(3 D PCRAM), invented by Intel and Micron, has been regarded as a new breakthrough in the last 25 years since the application of NAND in 1989, which represents state-of-the-art memory technology.This technology has some remarkable features, such as the confined device structure with 20 nm size, the metal crossbar electrodes to reduce the resistance variations in PCRAM arrays, and the ovonic threshold switching selector that can provide a high drive current and a low leakage current. A good understanding of phase change mechanism is of great help to design new phase change materials with fast operation speed,low power consumption and long-lifetime. In this paper, we firstly review the development of PCRAM and different understandings on phase change mechanisms in recent years, and then propose a new view on the mechanism, which is based on the octahedral structure motifs and vacancies. Octahedral structure motifs are generally found in both amorphous and crystalline phase change materials. They are considered to be the basic units during phase transition, which are severely defective in the amorphous phase. These configurations turn into more ordered ones after minor local rearrangements, the growth of which results in the crystallization of rocksalt(RS) phase with a large amount of vacancies in the cation sites. Further driven by thermodynamic driving force, these vacancies move and layer along certain directions; consequently, the metastable RS structure transforms into the stable hexagonal(HEX) structure. Based on our results, we find that reversible phase transition between amorphous phase and RS phase, without further changing into HEX phase, would greatly decrease the required power consumption. Robust octahedra and plenty of vacancies in both amorphous and RS phase, respectively avoiding large atomic rearrangement and providing necessary space, are crucial to achieve the nanosecond or even sub-nanosecond operation of PCRAM. | Zhitang SONG Sannian SONG Min ZHU Liangcai WU Kun REN Wenxiong SONG Songling FENG | 2018 | Science China(Information Sciences)2018，61，8： | 3 |
| 3 | Characterization of polyclonal antibodies against nonstructural protein 9 from the porcine reproductive and respiratory syndrome virus显示文摘Porcine reproductive and respiratory syndrome(PRRS) is considered to be one of the most important infectious diseases impacting the swine industry and is characterized by reproductive failure in late term gestation in sows and respiratory disease in pigs of all ages.The nonstructural protein 9 gene,Nsp9,encoding the RNA-dependent RNA polymerase,is generally regarded as fairly conserved when compared to other viral proteins.Antibodies against Nsp9 will be of great importance for the diagnosis and treatment of the causal agent,PRRS virus.A study was undertaken to generate polyclonal antibodies against the immunodominant Nsp9.For this purpose,the Nsp9 was expressed in Escherichia coli and subsequently used as an antigen to immunize New Zealand rabbits.Antiserum was identified via an indirect ELISA,and then verified based on the ability to react with both naturally and artificially expressed Nsp9.Results of virus neutralization test showed that this antiserum could not neutralize the PRRSV.Nevertheless,this antiserum as a diagnostic core reagent should prove invaluable for further investigations into the mechanism of PRRS pathogenesis. | Mengmeng ZHAO Juanjuan QIAN Jiexiong XIE Tiantian CUI Songling FENG Guoqiang WANG Ruining WANG Guihong ZHANG | 2016 | Frontiers of Agricultural Science and Engineering2016，3，2： | 2 |
| 4 | Allometric models for aboveground biomass of six common subtropical shrubs and small trees显示文摘The aboveground biomass(AGB)of shrubs and small trees is the main component for the productivity and carbon storage of understory vegetation in subtropical secondary forests.However,few allometric models exist to accurately evaluate understory biomass.To estimate the AGB of five common shrub(diameter at base<5 cm,<5 m high)and one small tree species(<8 m high,trees’s seedling),206 individuals were harvested and species-specific and multi-species allometric models developed based on four predictors,height(H),stem diameter(D),crown area(Ca),and wood density(ρ).As expected,the six species possessed greater biomass in their stems compared with branches,with the lowest biomass in the leaves.Species-specific allometric models that employed stem diameter and the combined variables of D~2H andρDH as predictors accurately estimated the components and total AGB,with R^(2) values from 0.602 and 0.971.A multi-species shrub allometric model revealed that wood density×diameter×height(ρDH)was the best predictor,with R^(2) values ranging from between 0.81 and 0.89 for the components and total AGB,respectively.These results indicated that height(H)and diameter(D)were effective predictors for the models to estimate the AGB of the six species,and the introduction of wood density(ρ)improved their accuracy.The optimal models selected in this study could be applied to estimate the biomass of shrubs and small trees in subtropical regions. | Cheng Huang Chun Feng Yuhua Ma Hua Liu Zhaocheng Wang Shaobo Yang Wenjing Wang Songling Fu Han Y.H.Chen | 2022 | Journal of Forestry Research2022，33，4： | 1 |
| 5 | Water use patterns and agronomic performance for some cropping systems with and without fallow crops in a semi-arid environment of northwest China显示文摘 | FENG RUI L SONGLING Z GEBALLE G T | 2000 | Agricu- lture Ecosystems and Environment2000，79，2： | 1 |
| 6 | Designing and Optimization of Fuzzy Sliding Mode Controller for Nonlinear Systems显示文摘For enhancing the control effectiveness,we firstly design a fuzzy logic based sliding mode controller(FSMC)for nonlinear crane systems.On basis of overhead crane dynamic characteristic,the sliding mode function with regard to trolley position and payload angle.Additionally,in order to eliminate the chattering problem of sliding mode control,the fuzzy logic theory is adopted to soften the control performance.Moreover,aiming at the FSMC parameter setting problem,a DE algorithm based optimization scheme is proposed for enhancing the control performance.Finally,by implementing the computer simulation,the DE based FSMC can effectively tackle the overhead crane sway problem and avoid unexpected accident greatly. | Zhe Sun Yunrui Bi Songle Chen Bing Hu Feng Xiang Yawen Ling Zhixin Sun | 2019 | Computers, Materials & Continua2019，，7： | 0 |
| 7 | Accurate Online MTPA Control of IPMSM Considering Derivative Terms显示文摘The conventional maximum torque per ampere(MTPA)operation usually neglects the derivative terms of interior permanent magnet synchronous motor(IPMSM)parameters,which significantly influences MTPA control accuracy.In this study,an MTPA control scheme that considers the derivative terms is developed,and a parameter identification strategy that considers the inverter to be non-ideal is developed for the calculation of the IPMSM parameters and derivative terms.In addition,the estimation accuracy of the motor parameters is further improved through the calibration of the nonlinear factors of the inverter.Finally,the effectiveness and accuracy of the proposed method is verified by simulation.This paper proposes practical methods for both inverter parameter estimation and accurate online MTPA control. | Riyang Yang Tianfu Sun Wei Feng Shaojia He Songling Zhu Xiao Chen | 2021 | Chinese Journal of Electrical Engineering2021，7，3： | 0 |
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