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| 1 | Antineoplastic effects of deoxyelephantopin,a sesquiterpene lactone from Elephantopusscaber, on lung adenocarcinoma (A549) cells显示文摘OBJECTIVE: Deoxyelephantopin, a sesquiterpene lactone from Elephantopus scaber, showed inhibition of the growth of various tumor cells in vitro. In the present study, we investigated the cytotoxicity and apoptosis-inducing capacity of deoxyelephantopin on lung adenocarcinoma (A549) cells. METHODS: The cytotoxic effect of deoxyelephantopin on A549 cells and normal lymphocytes was evaluated using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and 50% inhibitory concentration (IC50) value was determined. The self-renewal and proliferating potential of A549 cells after treatment with deoxyelephantopin were examined by colony formation assay. Cellular morphology of deoxyelephantopin-treated cells was observed using phasecontrast microscopy. The induction of apoptosis was evaluated using acridine orange and ethidium bromide staining, Hoechst 33342 staining, terminal deoxynucleotidyl transferase-mediated dUTP biotin nick end-labeling (TUNEL) assay, DNA fragmentation analysis and Annexin V-fluorescein isothiocyanate staining by flow cytometry. Activation of caspases was detected using fluorogenic substrate specific to caspases 2, 3, 8 and 9 and flow cytometric analysis. The total cellular DNA content and expression of cleaved poly (ADP-ribose) polymerase was also analyzed. RESULTS: Deoxyelephantopin exhibited cytotoxicity to A549 cells (IC50=12.287 μg/mL), however, there was no toxicity towards normal human lymphocytes. Deoxyelephantopin suppressed the colony-forming ability of A549 cells in a dose-dependent manner. Acridine orange, ethidium bromide and Hoechst 33342 staining showed cell shrinkage, chromosomal condensation and nuclear fragmentation, indicating induction of apoptosis. Deoxyelephantopin increased apoptosis of A549 cells, as evidenced by more TUNEL-positive cells. DNA fragmentation and Annexin V staining revealed late-stage apoptotic cell population. Deoxyelephantopin inhibited A549 cell growth by cell cycle arrest at G2/M phase and induced apoptosis through both extrinsic and intrinsic pathways. CONCLUSION: These results suggest that deoxyelephantopin has great potential as a new chemotherapeutic agent to be developed further for the treatment of lung cancer. | Farha A. Kabeer Geetha B. Sreedevi Mangalam S. Nair Dhanya S. Rajalekshmi LathaP. Gopalakrishnan Sujathan Kunjuraman Remani Prathapan | 2013 | Journal of Integrative Medicine2013，11，4： | 4 |
| 2 | Defect inspection technologies for additive manufacturing显示文摘Additive manufacturing(AM) technology is considered one of the most promising manufacturing technologies in the aerospace and defense industries. However, AM components are known to have various internal defects, such as powder agglomeration, balling, porosity,internal cracks and thermal/internal stress, which can significantly affect the quality, mechanical properties and safety of final parts. Therefore, defect inspection methods are important for reducing manufactured defects and improving the surface quality and mechanical properties of AM components. This paper describes defect inspection technologies and their applications in AM processes. The architecture of defects in AM processes is reviewed. Traditional defect detection technology and the surface defect detection methods based on deep learning are summarized, and future aspects are suggested. | Yao Chen Xing Peng Lingbao Kong Guangxi Dong Afaf Remani Richard Leach | 2021 | International Journal of Extreme Manufacturing2021，3，2： | 2 |
| 3 | Differential espression of jackfmit lectin specific glycoccon jttgates in metastatic adenocarcinoma and reactive mesothelial a diadnosis aid in effusion cytology显示文摘 | Sujathan K Kannan S Remani P | 1996 | J Cancer Res Clinocol1996，122，7： | 1 |
| 4 | Immunoglobulins in the sali-va of diabetic patients with periodontitis显示文摘 | Anil S Remani P Beena VT | 1995 | Ann Dent1995，54，12： | 1 |
| 5 | Growth of monodisperse nanocrystals of cerium oxide during synthesis and annealing显示文摘 | Swapankumar Ghosh Damodaran Divya Kottayilpadi C. Remani Thadathil S. Sreeremya | 2010 | Journal of Nanoparticle Research2010，，5： | 1 |
| 6 | Loss of chromo- some Y in acute lymphoblastic leukemia: age related or neoplastic phenomenon? 显示文摘 | Gupta A Parihar M Remani AS | 2014 | Indian J Pathol Mierobiol2014，57，3： | 1 |
| 7 | Transperitoneal veraus retroperitoneal laparoscopic partial nephrectomy: patient selection and perioperative outcomes显示文摘 | NG CS GILL IS REMANI AP | 2005 | J Urol2005，174，3： | 1 |
| 8 | Antitumour Effects of Isocurcumenol Isolated from Curcuma zedoaria Rhizomes on Human and Murine Cancer Cells显示文摘 | S. Lakshmi G. Padmaja P. Remani Armando Rossello | 2011 | International Journal of Medicinal Chemistry2011，，： | 1 |
| 9 | Immunoglobulins in the saliva of diabetic patients with periodotitis显示文摘 | Anil S Remani P Beena VT | 1995 | Diabetes1995，54，12： | 1 |
| 10 | Growth,repetitive breeding,and aquaculture potential of thespiny lobster,Panulirus ornatus显示文摘 | Senthil Murugan T Remany M C Mary Leema T | 2005 | New Zealand Journal ofMarine and Freshwater Research2005，39，： | 1 |
| 11 | Immunoglobulins in the saliva of diabetic patients with periodotitis 显示文摘 | Anil S Remani P Beena VT | 1995 | Diabetes1995，54，12： | 1 |
| 12 | Evaluation of Elephantopus scaber on the inhibition of chemical carcinogenesis and tumor development in mice显示文摘 | Geetha BS Latha PG Remani P | | 0，，03： | 1 |
| 13 | Transperitoneal veraus retroperito- neal laparoscopic partial nephrectomy: patient selection and periopera- tive outcomes 显示文摘 | Ng CS Gill IS Remani AP | 2005 | J Uml2005，174，3： | 1 |
| 14 | Differential expression of jackfruit lectin specific glycoconjugatos in metastatic adenocarcinoma and reactive mesothelial cell a diagnosis aid in effusion cytology显示文摘 | Sujathan K Kannan S Remani P | 1996 | J Cancer Bes Clin Oncol1996，122，7： | 1 |
| 15 | Antitumor constituents from Annona squamosa fruit pericarp 显示文摘 | Joy B Remani P | 2008 | Med Chem Res2008，17，27： | 1 |
| 16 | Captive breeding of the spiny lobster,Pcraulirus homarus显示文摘 | Vijayakumaran M Senthil Murugan T Remany M C | 2005 | New Zealand journal of marine and freshwater research2005，39，： | 1 |
| 17 | Captive breeding of the spiny lobster,Panulirus homarus显示文摘 | Senthil Murugan T Remany M C | 2005 | New Zealand journal of marine and freshwater research2005，39，： | 1 |
| 18 | Differential expression of jackfruit-leein-specific glycoconjugates in metastatic adenocarcinoma and reactive mesothelial cells-a diagnosis aid in effusion cytology 显示文摘 | Sujathan K Kannan S Remani P | 1996 | J Cancer Res Clin Oncol1996，122，7： | 1 |
| 19 | Certification Process of Imported Pacific White Shrimp Litopenaeus vannamei (Boone, 1931) at Aquatic Quarantine Facility, India显示文摘 | M. C. Remany DalyCyriac D. Kannanl, A. K. Panda Jaideep Kumar Y. C. Thampi Samraj | 2011 | Journal of Agricultural Science and Technology(B)2011，1，6： | 0 |
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