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| 1 | A next-generation marker genotyping platform(AmpSeq)in heterozygous crops:a case study for marker-assisted selection in grapevine显示文摘Marker-assisted selection(MAS)is often employed in crop breeding programs to accelerate and enhance cultivar development,via selection during the juvenile phase and parental selection prior to crossing.Next-generation sequencing and its derivative technologies have been used for genome-wide molecular marker discovery.To bridge the gap between marker development and MAS implementation,this study developed a novel practical strategy with a semi-automated pipeline that incorporates trait-associated single nucleotide polymorphism marker discovery,low-cost genotyping through amplicon sequencing(AmpSeq)and decision making.The results document the development of a MAS package derived from genotyping-by-sequencing using three traits(flower sex,disease resistance and acylated anthocyanins)in grapevine breeding.The vast majority of sequence reads(⩾99%)were from the targeted regions.Across 380 individuals and up to 31 amplicons sequenced in each lane of MiSeq data,most amplicons(83 to 87%)had<10%missing data,and read depth had a median of 220–244×.Several strengths of the AmpSeq platform that make this approach of broad interest in diverse crop species include accuracy,flexibility,speed,high-throughput,low-cost and easily automated analysis. | Shanshan Yang Jonathan Fresnedo-Ramíre Minghui Wang Linda Cote Peter Schweitzer Paola Barba Elizabeth M Takacs Matthew Clark James Luby David C Manns Gavin Sacks Anna Katharine Mansfield Jason Londo Anne Fennell David Gadoury Bruce Reisch Lance Cadle-Davidson Qi Sun | 2016 | Horticulture Research2016，3，1： | 2 |
| 2 | Minor furocoumarins of Murraya koenigii 显示文摘 | Adebajo A C Reisch J | 2000 | Fitoterapia2000，71，3： | 1 |
| 3 | Genetic testing for pheochromocytoma-associated syndromes显示文摘 | Pawlu C Bausch B Reisch N | 2005 | Ann Endocrinol (Paris)2005，66，3： | 1 |
| 4 | Fruit breeding volume Ⅱ 显示文摘 | Pratt C | 1996 | John wiley & Sons Inc1996，334，： | 1 |
| 5 | Controls on the stable isotope composition of seasonal growth bands in aragonitic freshwater bivalves (Unionidae) 显示文摘 | Dettman D L Reische A L Lohmann K C | 1999 | Geochim Cosmochim Aeta1999，63，78： | 1 |
| 6 | Controls on the stable isotope composition of seasonal growth bands in aragonitic fresh-water bivalves (unionidae)显示文摘 | REISCHE A K LOHMANN K C | 1999 | Geochimica et Cosmochimica Acta1999，63，： | 1 |
| 7 | Morphology and phenology of Scabiosa columbaria from mown and grazed habitats 显示文摘 | REISCH C POSCHLOD P | 2006 | Flora2006，206，： | 1 |
| 8 | Spatial genetic structure and clonal diversity in an alpine population of Salix herbacea ( Salicaceae ) 显示文摘 | REISCH C SCHURM S POSCHLOD P | 2007 | Ann Bot-London2007，99，： | 1 |
| 9 | Polyelectrolyte Multilayers Capped with Polyelectrolytes Bearing Phosphorylcholine and Triethylene Glycol Groups: Parameters Influencing Antifouling Properties显示文摘 | Reisch A Voegel J C Gonthier E Decher G Senger B Schaaf P Me?sini P J | | 0，，06： | 1 |
| 10 | Highly Active Double Metal Cyanide Catalysts 显示文摘 | Smith C P Reisch J W | 1992 | J Elast and Plast1992，24，4： | 1 |
| 11 | Controls on the stable isotope composition of seasonal growth bands in aragonitic fresh-water bivalves (unionidae)显示文摘 | Dettman D L Reische A K Lohmann K C | 1999 | Geochimica et Cosmochimica Acta1999，63，： | 1 |
| 12 | Thermoplastic Polyurethane Elastomers Made form High Molecular Weight Polyether Polyols显示文摘 | Simth C P Reisch J W O Connor J M | 1992 | Elastomers Plastics1992，24，10： | 1 |
| 13 | Molecular variation within and between ten populations of Primula farinosa ( Primulaceae ) along an altitudinal gradiem in the northern Alps显示文摘 | Reisch C Anke A Rohl M | 2005 | Basic and Applied Ecology2005，6，： | 1 |
| 14 | Screening of myopic LASIK patients with increased epithelial wound healing显示文摘 | Lohmann CP Winkler yon Mohrenfels C Reisch U | 2001 | Ophthahnologe2001，98，5： | 1 |
| 15 | Failure of sur- gery to improve outcome in hypertensive putaminal hemorrhage: a prospective randomized trial 显示文摘 | BATJER H H REISCH J S ALLEN B C | 1990 | Arch Neurol1990，47，10： | 1 |
| 16 | Attempted pollen-mediated plant transformation employing genomic donor DNA显示文摘 | Sanford J C Reisch B I Reisch B I | 1985 | Theoretical and Applied Genetics1985，69，： | 1 |
| 17 | Attempted Pollen-mediated plant transformation employing genomic donor DNA显示文摘 | Sanford J C Skubik K A Reisch B I | 1985 | Theoretical and Applied Genetics1985，69，： | 1 |
| 18 | Controls on the stable isotope composition of seasonal growth bands in aragonitic fresh-water bivalves (unionidae)显示文摘 | Dettman D L reische A K Lohmann K C | 1999 | Geochimica et Cosmochimica Acta1999，63，： | 1 |
| 19 | Controls on the stable isotope composition of seasonal growth bands in aragonitie fresh-water bivalves (unionidae) 显示文摘 | Dettman D L Reische A K Lohmann K C | 1999 | Geochimica et Cosmochimica Acta1999，63，： | 1 |
| 20 | Spatial genetic structure and clonal diversity in an alpine population of Salix herbacea ( Salicaceae) 显示文摘 | Reisch C Schurm S Poschlod P | 2007 | Ann Bot ( Loud )2007，99，4： | 1 |
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